MD11G1153800.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016527
Physical Location & Seq
Reverse (-)
14741837 .. 14745276
3440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1153800.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAGAAAGAAAATGGGATCATTAGTTTCTTTCCTTCTTGTGATTATAGCATTTTCTCTGCAGGCAAATGGGCAGCCCTTGGTTCCTGCAATGTTCATATTTGGGGACTCAGTTGTGGATGCTGGTAACAACAACCACCTCTACACCGTGATCAAAGCCAACTTCCCTCCTTATGGAAGAGACTTTGTCAATCACAAGCCAACTGGGAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGATTTACTTCTTACCCTCCAGCTTATCTTAGCAAAGAAGCCAATGGAACAAACCTCTTGATTGGTGCCAATTTTGGCTCAGCTGGTTCTGGCTATTATGAGTATACAGCAAAATTATATGATGTAATTTCACTAAATCAGCAGCTGGAGTACTACAAGGAATACCAAAACAAGGTGGTGGGAATTGCAGCACGAAATAAAGCCAATTTTACTTCAATAATATCTGATGCAGTCTACCTTGTTGGTGCTGGGAGCAGTGACTTTCTTCAGAACTACTACATTGATCCTCTTCTTTACAAGTTCTATACGCCCAACCAGTTCTCTGACATTCTCATGCAAGCTTATGCAACATTCATACAGAATCTATATGCACTGGGAGCAAGGAGAATCGGAGTGACCACATTGCCGCCACTTGGATGCCTGCCGGCGGCCATCACCATATTTGGGTCGCACAGCAATGAGTGCGTGGATAAAGTCAACAATGATGCAATCTCATTCAATAACAAGCTCAATGCCACATCTCAGAGTTTGCAAAATGACCTCTCTGGTCTTAAGTTGGTCGTCCTTGATATCTACCAACCGCTCTATGACCTTGTCACAAAGCCTGCCGAGAATGGGTTTGCGGAGGCAAGGCGGGCTTGTTGTGGAACAGGGCTGGTAGAAACAGCAATACTGTGCAATGCAGAGTCAGTAGGGACCTGCGCCAATGCGTCTGAGTATGTCTTTTGGGATGGCTTTCATCCTTCTGAGGCTGCAAACCAAATTTTGGCCAATGATTTGCTCAGCAGTGGCATCTCCCTCATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

38.49

Weight (kDa)

5.41

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 344 2.2e-38 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 1 cut(s) 1021
AccBSI CCGCTC 1 cut(s) 838
AccI GTMKAC 2 cut(s) 359, 489
AciI CCGC 5 cut(s) 662, 683, 836, 878, 889
AclWI GGATC 2 cut(s) 25, 533
AcoI YGGCCR 2 cut(s) 684, 1023
AcsI RAATTY 1 cut(s) 1017
AcuI CTGAAG 2 cut(s) 216, 506
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 7 cut(s) 173, 427, 668, 682, 699, 870, 1021
AflII CTTAAG 1 cut(s) 806
AgsI TTSAA 2 cut(s) 471, 754
AluBI AGCT 6 cut(s) 226, 278, 338, 400, 596, 763
AluI AGCT 6 cut(s) 226, 278, 338, 400, 596, 763
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 2 cut(s) 25, 533
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 2 cut(s) 684, 1023
ApeKI GCWGC 4 cut(s) 73, 397, 443, 1007
ApoI RAATTY 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 959
AspS9I GGNCC 1 cut(s) 951
AsuHPI GGTGA 1 cut(s) 682
AvaII GGWCC 1 cut(s) 951
BalI TGGCCA 1 cut(s) 1025
BanI GGYRCC 1 cut(s) 320
BbvI GCAGC 4 cut(s) 85, 409, 455, 994
BccI CCATC 2 cut(s) 695, 980
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 806
BfuAI ACCTGC 1 cut(s) 962
BisI GCNGC 6 cut(s) 74, 398, 444, 662, 684, 1008
BlpI GCTNAGC 2 cut(s) 334, 1037
BlsI GCNGC 6 cut(s) 75, 399, 445, 663, 685, 1009
BmcAI AGTACT 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 951
BmgT120I GGNCC 1 cut(s) 951
BmiI GGNNCC 3 cut(s) 84, 322, 952
BmrI ACTGGG 2 cut(s) 213, 638
BmsI GCATC 5 cut(s) 109, 472, 662, 730, 1056
BmuI ACTGGG 2 cut(s) 213, 638
BpmI CTGGAG 2 cut(s) 258, 422
Bpu1102I GCTNAGC 2 cut(s) 334, 1037
BsaJI CCNNGG 2 cut(s) 78, 253
Bsc4I CCNNNNNNNGG 7 cut(s) 173, 427, 668, 682, 699, 870, 1021
Bse118I RCCGGY 1 cut(s) 679
Bse1I ACTGG 3 cut(s) 208, 571, 633
Bse3DI GCAATG 5 cut(s) 96, 223, 656, 718, 940
BseDI CCNNGG 2 cut(s) 78, 253
BseGI GGATG 4 cut(s) 124, 677, 991, 994
BseLI CCNNNNNNNGG 7 cut(s) 173, 427, 668, 682, 699, 870, 1021
BseMI GCAATG 5 cut(s) 96, 223, 656, 718, 940
BseMII CTCAG 6 cut(s) 123, 348, 791, 960, 993, 1051
BseNI ACTGG 3 cut(s) 208, 571, 633
BseXI GCAGC 4 cut(s) 85, 409, 455, 994
BseYI CCCAGC 1 cut(s) 503
BshFI GGCC 2 cut(s) 686, 1025
BshNI GGYRCC 1 cut(s) 320
BsiSI CCGG 1 cut(s) 680
BslFI GGGAC 2 cut(s) 119, 964
BslI CCNNNNNNNGG 7 cut(s) 173, 427, 668, 682, 699, 870, 1021
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 2 cut(s) 119, 964
BsnI GGCC 2 cut(s) 686, 1025
Bsp143I GATC 3 cut(s) 17, 150, 538
Bsp1720I GCTNAGC 2 cut(s) 334, 1037
BspACI CCGC 5 cut(s) 662, 683, 836, 878, 889
BspANI GGCC 2 cut(s) 686, 1025
BspCNI CTCAG 6 cut(s) 122, 347, 790, 961, 994, 1050
BspLI GGNNCC 3 cut(s) 84, 322, 952
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 962
BspPI GGATC 2 cut(s) 25, 533
BspT107I GGYRCC 1 cut(s) 320
BspTI CTTAAG 1 cut(s) 806
BsrBI CCGCTC 1 cut(s) 838
BsrDI GCAATG 5 cut(s) 96, 223, 656, 718, 940
BsrFI RCCGGY 1 cut(s) 679
BsrI ACTGG 3 cut(s) 208, 571, 633
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 2 cut(s) 78, 253
BssMI GATC 3 cut(s) 17, 150, 538
BssNAI GTATAC 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 78, 253
Bst1107I GTATAC 1 cut(s) 360
Bst4CI ACNGT 2 cut(s) 148, 930
Bst6I CTCTTC 2 cut(s) 172, 549
BstAFI CTTAAG 1 cut(s) 806
BstAPI GCANNNNNTGC 1 cut(s) 717
BstC8I GCNNGC 7 cut(s) 63, 228, 594, 677, 681, 861, 891
BstDEI CTNAG 7 cut(s) 109, 284, 334, 777, 969, 1002, 1037
BstF5I GGATG 4 cut(s) 124, 677, 991, 994
BstHHI GCGC 1 cut(s) 959
BstKTI GATC 3 cut(s) 20, 153, 541
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 3 cut(s) 17, 150, 538
BstMWI GCNNNNNNNGC 3 cut(s) 632, 717, 890
BstSFI CTRYAG 1 cut(s) 59
BstV1I GCAGC 4 cut(s) 85, 409, 455, 994
BstZ17I GTATAC 1 cut(s) 360
BsuRI GGCC 2 cut(s) 686, 1025
BtsCI GGATG 4 cut(s) 124, 677, 991, 994
BtsI GCAGTG 3 cut(s) 240, 517, 1048
BtsIMutI CAGTG 4 cut(s) 240, 517, 626, 1048
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 7 cut(s) 63, 228, 594, 677, 681, 861, 891
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 1 cut(s) 959
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 1 cut(s) 951
CseI GACGC 1 cut(s) 954
Csp6I GTAC 1 cut(s) 406
CviAII CATG 1 cut(s) 589
CviQI GTAC 1 cut(s) 406
DdeI CTNAG 7 cut(s) 109, 284, 334, 777, 969, 1002, 1037
DpnI GATC 3 cut(s) 19, 152, 540
DpnII GATC 3 cut(s) 17, 150, 538
EaeI YGGCCR 2 cut(s) 684, 1023
Eam1104I CTCTTC 2 cut(s) 172, 549
EarI CTCTTC 2 cut(s) 172, 549
Eco130I CCWWGG 2 cut(s) 78, 253
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 951
Eco57I CTGAAG 2 cut(s) 216, 506
EcoO109I RGGNCCY 1 cut(s) 951
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 78, 253
ErhI CCWWGG 2 cut(s) 78, 253
FaeI CATG 1 cut(s) 592
FalI AAGNNNNNCTT 2 cut(s) 877, 909
FaqI GGGAC 2 cut(s) 119, 964
FatI CATG 1 cut(s) 588
FauI CCCGC 1 cut(s) 882
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 2 cut(s) 359, 489
Fnu4HI GCNGC 6 cut(s) 74, 398, 444, 662, 684, 1008
FokI GGATG 4 cut(s) 131, 684, 981, 998
Fsp4HI GCNGC 6 cut(s) 74, 398, 444, 662, 684, 1008
GlaI GCGC 1 cut(s) 958
GluI GCNGC 6 cut(s) 74, 398, 444, 662, 684, 1008
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 2 cut(s) 258, 422
HaeIII GGCC 2 cut(s) 686, 1025
HapII CCGG 1 cut(s) 680
HgaI GACGC 1 cut(s) 954
HhaI GCGC 1 cut(s) 959
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 1 cut(s) 957
HinP1I GCGC 1 cut(s) 957
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HindIII AAGCTT 2 cut(s) 224, 594
HinfI GANTC 4 cut(s) 107, 616, 642, 941
HpaII CCGG 1 cut(s) 680
HphI GGTGA 1 cut(s) 682
Hpy166II GTNNAC 3 cut(s) 360, 490, 733
Hpy188I TCNGA 8 cut(s) 235, 481, 525, 580, 647, 780, 970, 1003
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 3 cut(s) 360, 490, 733
HpyAV CCTTC 2 cut(s) 44, 1008
HpyCH4III ACNGT 2 cut(s) 148, 930
HpyF10VI GCNNNNNNNGC 3 cut(s) 632, 717, 890
HpyF3I CTNAG 7 cut(s) 109, 284, 334, 777, 969, 1002, 1037
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 1 cut(s) 957
KroI GCCGGC 1 cut(s) 679
KroNI GCCGGC 1 cut(s) 681
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 3 cut(s) 17, 150, 538
LmnI GCTCC 2 cut(s) 507, 632
Lsp1109I GCAGC 4 cut(s) 85, 409, 455, 994
LweI GCATC 5 cut(s) 109, 472, 662, 730, 1056
MaeIII GTNAC 4 cut(s) 125, 512, 649, 850
MalI GATC 3 cut(s) 19, 152, 540
MbiI CCGCTC 1 cut(s) 838
MboI GATC 3 cut(s) 17, 150, 538
MboII GAAGA 3 cut(s) 189, 512, 536
MlsI TGGCCA 1 cut(s) 1025
MluCI AATT 6 cut(s) 325, 368, 381, 438, 460, 1017
MluNI TGGCCA 1 cut(s) 1025
MlyI GAGTC 2 cut(s) 101, 950
Mox20I TGGCCA 1 cut(s) 1025
MroNI GCCGGC 1 cut(s) 679
MscI TGGCCA 1 cut(s) 1025
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 3 cut(s) 587, 670, 711
Msp20I TGGCCA 1 cut(s) 1025
MspA1I CMGCKG 2 cut(s) 338, 400
MspCI CTTAAG 1 cut(s) 806
MspI CCGG 1 cut(s) 680
MwoI GCNNNNNNNGC 3 cut(s) 632, 717, 890
NaeI GCCGGC 1 cut(s) 681
NdeII GATC 3 cut(s) 17, 150, 538
NgoMIV GCCGGC 1 cut(s) 679
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 3 cut(s) 84, 322, 952
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 3 cut(s) 512, 649, 850
PdiI GCCGGC 1 cut(s) 681
PfeI GAWTC 2 cut(s) 616, 642
PflFI GACNNNGTC 2 cut(s) 185, 848
PflMI CCANNNNNTGG 1 cut(s) 1021
PkrI GCNGC 6 cut(s) 75, 399, 445, 663, 685, 1009
PleI GAGTC 2 cut(s) 101, 949
PpsI GAGTC 2 cut(s) 101, 949
PpuMI RGGWCCY 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 951
PspFI CCCAGC 1 cut(s) 503
PspN4I GGNNCC 3 cut(s) 84, 322, 952
PspPI GGNCC 1 cut(s) 951
PspPPI RGGWCCY 1 cut(s) 951
PstI CTGCAG 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 400
PsyI GACNNNGTC 2 cut(s) 185, 848
PvuII CAGCTG 2 cut(s) 338, 400
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
RseI CAYNNNNRTG 3 cut(s) 587, 670, 711
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 6 cut(s) 74, 398, 444, 662, 684, 1008
Sau3AI GATC 3 cut(s) 17, 150, 538
Sau96I GGNCC 1 cut(s) 951
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 2 cut(s) 101, 950
SfaNI GCATC 5 cut(s) 109, 472, 662, 730, 1056
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 951
SmiMI CAYNNNNRTG 3 cut(s) 587, 670, 711
SmlI CTYRAG 1 cut(s) 806
SmoI CTYRAG 1 cut(s) 806
Sse9I AATT 6 cut(s) 325, 368, 381, 438, 460, 1017
SsiI CCGC 5 cut(s) 662, 683, 836, 878, 889
StyI CCWWGG 2 cut(s) 78, 253
TaaI ACNGT 2 cut(s) 148, 930
TasI AATT 6 cut(s) 325, 368, 381, 438, 460, 1017
TatI WGTACW 1 cut(s) 405
TauI GCSGC 2 cut(s) 664, 686
TfiI GAWTC 2 cut(s) 616, 642
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 4 cut(s) 247, 517, 633, 1048
TseFI GTSAC 3 cut(s) 512, 649, 850
TseI GCWGC 4 cut(s) 73, 397, 443, 1007
Tsp45I GTSAC 3 cut(s) 512, 649, 850
TspDTI ATGAA 3 cut(s) 85, 598, 983
TspRI CASTG 4 cut(s) 247, 517, 633, 1048
Tth111I GACNNNGTC 2 cut(s) 185, 848
Van91I CCANNNNNTGG 1 cut(s) 1021
Vha464I CTTAAG 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 951
XapI RAATTY 1 cut(s) 1017
XmiI GTMKAC 2 cut(s) 359, 489
ZrmI AGTACT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.