RchiOBHm_Chr5g0055911

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016527
Physical Location & Seq
Forward (+)
59277431 .. 59280196
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33285

Sequence Viewer

Length: 1260 bp
ATGCAAATCTTGTTTAATGTATATAATTTGGATGTAAGTCAATGTAACACAAACTTACTCTTCTGGCTGTATCTTTTCCTGTTAGAATCATCGATCTATCATCGATCGTTTCTCAGCACATCAGCTATTGTTTCATTGACTTCTTTTCGGAGGTCTTCAGCCAACTTTCATGTTGTCTACTTCCAGTTTAAGCTTGATCGACAATTGTTTGCTACTTTGCATTTATCTGATGTAGTGCTCTATGATGTGGGTGATGGGCAGCCTTTGGTTCCTGCAATGTTCATATTTGGAGACTCAGTTGTGGATGTGGGTAATAACAACAACCTCTTCTCCTTAATCAAATCCAACTTCCCCCCTTATGGAAGAGACTTTGTCAATCACAATCCCACCGGCAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGCTTTGCTTCTCACCAACCAGCTTATCTCAGCGGAGAAGCCACAGGAAAAAGCCTCTTGATTGGTGCCAACTTTGCTTCAGCTGGCTCTGGCTATTATGACGCTACAGCAAAGTTATTTAATGCAATTTCACTGAGCCAGCAGGTGGAGTACTACAAGGAATATCAAAATAAGGTGGTGGGAATTGGAGGAAAAGTGAATGCTACATTAATTATATCGGGTGCAATATACCTGGTTAGTGCTGGGAGCAGTGATTTTGTTCAGAACTACTACATCAATCCTCTTCTTAACAAGGTCTATACGACAGACCAGTTCTCTAACATTCTCATGCAGTCCTATGAAAACTTCATACAGGCAAGTCCTTATCGTAATCTATATGCCTTGGGAGCACGCAGAATCGGAGTGACCACCCTGCCACCACTTGGATGTCTGCCGGCGGCCATCACAATATTTGGATTAGGCGGCAACAAGTGCGTGGCTAAGCTTAATGGCGATGCAGTCTCATTTAACAATAAGCTAAATGCCACATCTCAATACTTGCAAACTAAGCTGCCCGGACTCAAGTTGGTCGTCTTTGACATCTACCAGCCTCTCTACAGCCTTGTCATGAAGCCTTCAGAGAATGGGTTCTCAGAGGCGAGGAGGGCTTGTTGTGGATCAGGGTTGCTGGAAACATCAATACTATGCAATGCCAAGTCCACCGGCACATGCGCCAATGCGTCAGAGTATGTGTTCTGGGATGGGTTTCACCCTTCGGAGGCCGCAAACAAAATTTTAGCAGATGATTTGCTGAGTAGTGGCATCTCTCTCATCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

419

Amino Acids

45.89

Weight (kDa)

6.98

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 94 - 409 2.3e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
Acc36I ACCTGC 2 cut(s) 384, 574
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 2 cut(s) 586, 863
AccI GTMKAC 1 cut(s) 177
AciI CCGC 4 cut(s) 474, 878, 903, 1203
AclWI GGATC 1 cut(s) 1105
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 1 cut(s) 1212
AcuI CTGAAG 4 cut(s) 141, 402, 504, 1041
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 5 cut(s) 359, 393, 586, 863, 1198
AjnI CCWGG 1 cut(s) 672
AluBI AGCT 8 cut(s) 125, 193, 412, 464, 524, 925, 958, 991
AluI AGCT 8 cut(s) 125, 193, 412, 464, 524, 925, 958, 991
Alw21I GWGCWC 2 cut(s) 240, 832
Alw26I GTCTC 3 cut(s) 285, 360, 946
AlwI GGATC 1 cut(s) 1105
AlwNI CAGNNNCTG 1 cut(s) 399
AoxI GGCC 2 cut(s) 879, 1200
ApeKI GCWGC 2 cut(s) 259, 991
ApoI RAATTY 1 cut(s) 1212
AseI ATTAAT 1 cut(s) 650
Asp700I GAANNNNTTC 1 cut(s) 1067
AspLEI GCGC 1 cut(s) 1154
AsuC2I CCSGG 1 cut(s) 996
AsuHPI GGTGA 3 cut(s) 263, 446, 1181
BanI GGYRCC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 240, 832
BbvI GCAGC 2 cut(s) 271, 978
BccI CCATC 3 cut(s) 248, 890, 1175
BciT130I CCWGG 1 cut(s) 674
BcnI CCSGG 1 cut(s) 996
BcoDI GTCTC 3 cut(s) 285, 360, 946
BfmI CTRYAG 2 cut(s) 546, 1036
BfuAI ACCTGC 2 cut(s) 384, 574
BisI GCNGC 5 cut(s) 260, 879, 904, 992, 1203
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 5 cut(s) 261, 880, 905, 993, 1204
BmcAI AGTACT 1 cut(s) 593
Bme1390I CCNGG 2 cut(s) 674, 996
BmiI GGNNCC 2 cut(s) 270, 508
BmrFI CCNGG 2 cut(s) 674, 996
BmsI GCATC 2 cut(s) 925, 1251
BpiI GAAGAC 1 cut(s) 147
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 986
BpuMI CCSGG 1 cut(s) 996
Bsa29I ATCGAT 2 cut(s) 92, 103
BsaJI CCNNGG 2 cut(s) 439, 822
BsaXI ACNNNNNCTCC 8 cut(s) 282, 312, 314, 344, 621, 651, 1075, 1105
Bsc4I CCNNNNNNNGG 5 cut(s) 359, 393, 586, 863, 1198
Bse118I RCCGGY 3 cut(s) 389, 874, 1142
Bse1I ACTGG 2 cut(s) 184, 751
Bse3DI GCAATG 3 cut(s) 282, 409, 1135
BseBI CCWGG 1 cut(s) 674
BseCI ATCGAT 2 cut(s) 92, 103
BseDI CCNNGG 2 cut(s) 439, 822
BseGI GGATG 4 cut(s) 37, 310, 872, 1186
BseLI CCNNNNNNNGG 5 cut(s) 359, 393, 586, 863, 1198
BseMI GCAATG 3 cut(s) 282, 409, 1135
BseMII CTCAG 6 cut(s) 127, 309, 484, 566, 1086, 1223
BseNI ACTGG 2 cut(s) 184, 751
BseRI GAGGAG 1 cut(s) 1096
BseXI GCAGC 2 cut(s) 271, 978
BseYI CCCAGC 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 881, 1202
BshNI GGYRCC 1 cut(s) 506
BshVI ATCGAT 2 cut(s) 92, 103
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 2 cut(s) 240, 832
BsiSI CCGG 4 cut(s) 390, 875, 996, 1143
BslI CCNNNNNNNGG 5 cut(s) 359, 393, 586, 863, 1198
BsmAI GTCTC 3 cut(s) 285, 360, 946
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 2 cut(s) 881, 1202
Bsp1286I GDGCHC 2 cut(s) 240, 832
Bsp143I GATC 4 cut(s) 93, 104, 196, 1097
Bsp1720I GCTNAGC 1 cut(s) 921
BspACI CCGC 4 cut(s) 474, 878, 903, 1203
BspANI GGCC 2 cut(s) 881, 1202
BspCNI CTCAG 6 cut(s) 126, 308, 483, 567, 1085, 1224
BspDI ATCGAT 2 cut(s) 92, 103
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 2 cut(s) 270, 508
BspMI ACCTGC 2 cut(s) 384, 574
BspPI GGATC 1 cut(s) 1105
BspT107I GGYRCC 1 cut(s) 506
BsrDI GCAATG 3 cut(s) 282, 409, 1135
BsrFI RCCGGY 3 cut(s) 389, 874, 1142
BsrI ACTGG 2 cut(s) 184, 751
BssAI RCCGGY 3 cut(s) 389, 874, 1142
BssECI CCNNGG 2 cut(s) 439, 822
BssMI GATC 4 cut(s) 93, 104, 196, 1097
BssT1I CCWWGG 2 cut(s) 439, 822
Bst2UI CCWGG 1 cut(s) 674
Bst6I CTCTTC 4 cut(s) 65, 332, 358, 729
BstAPI GCANNNNNTGC 2 cut(s) 399, 912
BstC8I GCNNGC 5 cut(s) 414, 526, 581, 832, 876
BstDEI CTNAG 8 cut(s) 113, 295, 470, 575, 921, 987, 1072, 1232
BstF5I GGATG 4 cut(s) 37, 310, 872, 1186
BstHHI GCGC 1 cut(s) 1154
BstKTI GATC 4 cut(s) 96, 107, 199, 1100
BstMAI GTCTC 3 cut(s) 285, 360, 946
BstMBI GATC 4 cut(s) 93, 104, 196, 1097
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 6 cut(s) 399, 515, 827, 912, 988, 1085
BstNI CCWGG 1 cut(s) 674
BstNSI RCATGY 1 cut(s) 1152
BstSCI CCNGG 2 cut(s) 672, 994
BstSFI CTRYAG 2 cut(s) 546, 1036
BstV1I GCAGC 2 cut(s) 271, 978
BstV2I GAAGAC 1 cut(s) 147
Bsu15I ATCGAT 2 cut(s) 92, 103
BsuRI GGCC 2 cut(s) 881, 1202
BsuTUI ATCGAT 2 cut(s) 92, 103
BtgZI GCGATG 1 cut(s) 948
BtsCI GGATG 4 cut(s) 37, 310, 872, 1186
BtsI GCAGTG 2 cut(s) 426, 697
BtsIMutI CAGTG 3 cut(s) 426, 572, 697
BveI ACCTGC 2 cut(s) 384, 574
Cac8I GCNNGC 5 cut(s) 414, 526, 581, 832, 876
CaiI CAGNNNCTG 1 cut(s) 399
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 1 cut(s) 1154
Cfr10I RCCGGY 3 cut(s) 389, 874, 1142
ClaI ATCGAT 2 cut(s) 92, 103
CseI GACGC 2 cut(s) 551, 1149
CsiI ACCWGGT 1 cut(s) 672
Csp6I GTAC 1 cut(s) 592
CviAII CATG 4 cut(s) 170, 769, 1048, 1149
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 8 cut(s) 113, 295, 470, 575, 921, 987, 1072, 1232
DpnI GATC 4 cut(s) 95, 106, 198, 1099
DpnII GATC 4 cut(s) 93, 104, 196, 1097
EaeI YGGCCR 1 cut(s) 879
Eam1104I CTCTTC 4 cut(s) 65, 332, 358, 729
EarI CTCTTC 4 cut(s) 65, 332, 358, 729
Eco130I CCWWGG 2 cut(s) 439, 822
Eco57I CTGAAG 4 cut(s) 141, 402, 504, 1041
EcoRII CCWGG 1 cut(s) 672
EcoT14I CCWWGG 2 cut(s) 439, 822
ErhI CCWWGG 2 cut(s) 439, 822
FaeI CATG 4 cut(s) 173, 772, 1051, 1152
FatI CATG 4 cut(s) 169, 768, 1047, 1148
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 5 cut(s) 260, 879, 904, 992, 1203
FokI GGATG 4 cut(s) 44, 317, 879, 1193
Fsp4HI GCNGC 5 cut(s) 260, 879, 904, 992, 1203
GlaI GCGC 1 cut(s) 1153
GluI GCNGC 5 cut(s) 260, 879, 904, 992, 1203
GsaI CCCAGC 1 cut(s) 687
HaeIII GGCC 2 cut(s) 881, 1202
HapII CCGG 4 cut(s) 390, 875, 996, 1143
HgaI GACGC 2 cut(s) 551, 1149
HhaI GCGC 1 cut(s) 1154
Hin1II CATG 4 cut(s) 173, 772, 1051, 1152
Hin6I GCGC 1 cut(s) 1152
HinP1I GCGC 1 cut(s) 1152
HindIII AAGCTT 3 cut(s) 191, 410, 923
HinfI GANTC 4 cut(s) 86, 293, 837, 999
HpaII CCGG 4 cut(s) 390, 875, 996, 1143
HphI GGTGA 3 cut(s) 263, 446, 1181
Hpy166II GTNNAC 2 cut(s) 178, 1140
Hpy188I TCNGA 9 cut(s) 150, 229, 421, 705, 842, 1060, 1075, 1165, 1198
Hpy188III TCNNGA 2 cut(s) 499, 1048
Hpy8I GTNNAC 2 cut(s) 178, 1140
HpyAV CCTTC 2 cut(s) 1065, 1203
HpyF10VI GCNNNNNNNGC 6 cut(s) 399, 515, 827, 912, 988, 1085
HpyF3I CTNAG 8 cut(s) 113, 295, 470, 575, 921, 987, 1072, 1232
Hsp92II CATG 4 cut(s) 173, 772, 1051, 1152
HspAI GCGC 1 cut(s) 1152
KroI GCCGGC 1 cut(s) 874
KroNI GCCGGC 1 cut(s) 876
Kzo9I GATC 4 cut(s) 93, 104, 196, 1097
LmnI GCTCC 2 cut(s) 687, 827
Lsp1109I GCAGC 2 cut(s) 271, 978
LweI GCATC 2 cut(s) 925, 1251
MabI ACCWGGT 1 cut(s) 672
MaeIII GTNAC 2 cut(s) 44, 844
MalI GATC 4 cut(s) 95, 106, 198, 1099
MboI GATC 4 cut(s) 93, 104, 196, 1097
MboII GAAGA 6 cut(s) 52, 147, 319, 375, 716, 1246
MfeI CAATTG 1 cut(s) 203
MhlI GDGCHC 2 cut(s) 240, 832
MluCI AATT 6 cut(s) 25, 203, 567, 624, 651, 1212
MlyI GAGTC 2 cut(s) 287, 993
MmeI TCCRAC 1 cut(s) 369
MroNI GCCGGC 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 1067
MseI TTAA 8 cut(s) 15, 189, 335, 561, 650, 729, 927, 948
MslI CAYNNNNRTG 2 cut(s) 767, 865
MspA1I CMGCKG 2 cut(s) 474, 524
MspI CCGG 4 cut(s) 390, 875, 996, 1143
MspR9I CCNGG 2 cut(s) 674, 996
MunI CAATTG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 1 cut(s) 674
MwoI GCNNNNNNNGC 6 cut(s) 399, 515, 827, 912, 988, 1085
NaeI GCCGGC 1 cut(s) 876
NciI CCSGG 1 cut(s) 996
NdeII GATC 4 cut(s) 93, 104, 196, 1097
NgoMIV GCCGGC 1 cut(s) 874
NlaIII CATG 4 cut(s) 173, 772, 1051, 1152
NlaIV GGNNCC 2 cut(s) 270, 508
NmuCI GTSAC 1 cut(s) 844
NspI RCATGY 1 cut(s) 1152
PagI TCATGA 1 cut(s) 1047
PaqCI CACCTGC 1 cut(s) 574
PctI GAATGC 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 876
PdmI GAANNNNTTC 1 cut(s) 1067
PfeI GAWTC 2 cut(s) 86, 837
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 2 cut(s) 586, 863
PkrI GCNGC 5 cut(s) 261, 880, 905, 993, 1204
Ple19I CGATCG 1 cut(s) 107
PleI GAGTC 2 cut(s) 287, 993
PpsI GAGTC 2 cut(s) 287, 993
PshBI ATTAAT 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 672
PspFI CCCAGC 1 cut(s) 683
PspGI CCWGG 1 cut(s) 672
PspN4I GGNNCC 2 cut(s) 270, 508
PstNI CAGNNNCTG 1 cut(s) 399
PsyI GACNNNGTC 1 cut(s) 371
PvuI CGATCG 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 2 cut(s) 767, 865
SaqAI TTAA 8 cut(s) 15, 189, 335, 561, 650, 729, 927, 948
SatI GCNGC 5 cut(s) 260, 879, 904, 992, 1203
Sau3AI GATC 4 cut(s) 93, 104, 196, 1097
ScaI AGTACT 1 cut(s) 593
SchI GAGTC 2 cut(s) 287, 993
ScrFI CCNGG 2 cut(s) 674, 996
SduI GDGCHC 2 cut(s) 240, 832
SexAI ACCWGGT 1 cut(s) 672
SfaNI GCATC 2 cut(s) 925, 1251
SfcI CTRYAG 2 cut(s) 546, 1036
SmiMI CAYNNNNRTG 2 cut(s) 767, 865
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
Sse9I AATT 6 cut(s) 25, 203, 567, 624, 651, 1212
SsiI CCGC 4 cut(s) 474, 878, 903, 1203
SspI AATATT 1 cut(s) 891
StyD4I CCNGG 2 cut(s) 672, 994
StyI CCWWGG 2 cut(s) 439, 822
TaqI TCGA 3 cut(s) 92, 103, 199
TasI AATT 6 cut(s) 25, 203, 567, 624, 651, 1212
TatI WGTACW 1 cut(s) 591
TauI GCSGC 3 cut(s) 881, 906, 1205
TfiI GAWTC 2 cut(s) 86, 837
Tru1I TTAA 8 cut(s) 15, 189, 335, 561, 650, 729, 927, 948
Tru9I TTAA 8 cut(s) 15, 189, 335, 561, 650, 729, 927, 948
TscAI CASTG 3 cut(s) 433, 579, 697
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 2 cut(s) 259, 991
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 6 cut(s) 123, 158, 271, 778, 795, 1064
TspRI CASTG 3 cut(s) 433, 579, 697
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 2 cut(s) 586, 863
VspI ATTAAT 1 cut(s) 650
XapI RAATTY 1 cut(s) 1212
XceI RCATGY 1 cut(s) 1152
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 1067
ZrmI AGTACT 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.