Rw5G034390

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016527
Physical Location & Seq
Forward (+)
59308914 .. 59310779
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034390.1

Sequence Viewer

Length: 1020 bp
ATGGGTTCTTTGTTACCTTGCTTTCTTCTTCTTCTAATTTCAGTGCTCTATGATGTGGGTGATGGGCAGCCTTTGGTTCCTGCAATGTTCATATTTGGAGACTCAGTTGTGGATGTGGGTAATAACAACAACCTCTTCTCCTTAATCAAATCCAACTTCCCTCCTTATGGAAGAGATTTTGTCAATCACAATCCCACCGGCAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGCTTTGCTTCTTACCAACCAGCTTATCTCAGCGGAGAAGCCACAGGAAAAAGCCTCTTGATTGGTGCCAACTTTGCTTCAGCTGGCTCTGGCTATTATGACGCTACAGCAAAGTTATTTAATGCAATTTCACTGAGCCAGCAGGTGGAGTACTACAAGGAATATCAAAATAAGGTGGTGGGAATTGGAGGAAAAGTGAATGCTACATTAATCATATCGGGTGCAATATACCTTGTTAGTGCTGGGAGCAGTGATTTTGTTCAGAACTACTACATCAATCCTCTTCTTAACAAGGTCTATACGACAGACCAGTTCTCTAATATTCTCATGCAGTCCTATGAAAACTTCATACAGAATCTATATGCCTTGGGAGCACGCAGAATCGGAGTGACCACCCTGCCACCACTTGGATGTCTGCCGGCGGCCATCACAATATTTGGATTAGGCGGCAACAAGTGCGTGGCTAAGCTTAATGGCGATGCAGTCTCATTTAACAATAAGCTAAATGCCACATCTCAGTACTTGCAAACTAAGCTGCCCGGACTCAAGTTGGTCGTCTTTGACATCTACCAGCCTCTCTACAACCTTGCGAGAAGGGCTTGTTGTGGATCAGGGTTGCTGGAAACATCAATACTATGCAATGCCAAGTCCACCGGCACATGCGCCAATGCGTCAGAGTATGTGTTCTGGGATGGTTTTCACCCTTCGGAGGCCGCAAACAAAATTTTAGCTGATGATTTGCTGAGTAGTGGCATCTCTCTCATCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

339

Amino Acids

36.45

Weight (kDa)

6.12

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 329 4.8e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
Acc36I ACCTGC 2 cut(s) 192, 382
AccB1I GGYRCC 1 cut(s) 314
AccB7I CCANNNNNTGG 2 cut(s) 394, 656
AciI CCGC 4 cut(s) 282, 671, 696, 963
AclWI GGATC 1 cut(s) 865
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 1 cut(s) 972
AcuI CTGAAG 2 cut(s) 210, 312
AfaI GTAC 2 cut(s) 401, 770
AfiI CCNNNNNNNGG 5 cut(s) 167, 201, 394, 656, 958
AluBI AGCT 7 cut(s) 220, 272, 332, 718, 751, 784, 980
AluI AGCT 7 cut(s) 220, 272, 332, 718, 751, 784, 980
Alw21I GWGCWC 2 cut(s) 48, 625
Alw26I GTCTC 2 cut(s) 93, 739
AlwI GGATC 1 cut(s) 865
AlwNI CAGNNNCTG 1 cut(s) 207
AoxI GGCC 2 cut(s) 672, 960
ApeKI GCWGC 2 cut(s) 67, 784
ApoI RAATTY 1 cut(s) 972
AseI ATTAAT 1 cut(s) 458
AspLEI GCGC 1 cut(s) 914
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 2 cut(s) 71, 941
BanI GGYRCC 1 cut(s) 314
Bbv12I GWGCWC 2 cut(s) 48, 625
BbvI GCAGC 2 cut(s) 79, 771
BccI CCATC 3 cut(s) 56, 683, 935
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 93, 739
BfmI CTRYAG 1 cut(s) 354
BfuAI ACCTGC 2 cut(s) 192, 382
BisI GCNGC 5 cut(s) 68, 672, 697, 785, 963
BlpI GCTNAGC 1 cut(s) 714
BlsI GCNGC 5 cut(s) 69, 673, 698, 786, 964
BmcAI AGTACT 2 cut(s) 401, 770
Bme1390I CCNGG 1 cut(s) 789
BmiI GGNNCC 2 cut(s) 78, 316
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 718, 1011
Bpu1102I GCTNAGC 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 1 cut(s) 789
BsaJI CCNNGG 2 cut(s) 247, 615
BsaXI ACNNNNNCTCC 6 cut(s) 90, 120, 122, 152, 429, 459
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 201, 394, 656, 958
Bse118I RCCGGY 3 cut(s) 197, 667, 902
Bse1I ACTGG 1 cut(s) 559
Bse3DI GCAATG 3 cut(s) 90, 217, 895
BseDI CCNNGG 2 cut(s) 247, 615
BseGI GGATG 3 cut(s) 118, 665, 946
BseLI CCNNNNNNNGG 5 cut(s) 167, 201, 394, 656, 958
BseMI GCAATG 3 cut(s) 90, 217, 895
BseMII CTCAG 5 cut(s) 117, 292, 374, 779, 983
BseNI ACTGG 1 cut(s) 559
BseXI GCAGC 2 cut(s) 79, 771
BseYI CCCAGC 1 cut(s) 491
BshFI GGCC 2 cut(s) 674, 962
BshNI GGYRCC 1 cut(s) 314
BsiHKAI GWGCWC 2 cut(s) 48, 625
BsiSI CCGG 4 cut(s) 198, 668, 789, 903
BslI CCNNNNNNNGG 5 cut(s) 167, 201, 394, 656, 958
BsmAI GTCTC 2 cut(s) 93, 739
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 2 cut(s) 674, 962
Bsp1286I GDGCHC 2 cut(s) 48, 625
Bsp143I GATC 1 cut(s) 857
Bsp1720I GCTNAGC 1 cut(s) 714
BspACI CCGC 4 cut(s) 282, 671, 696, 963
BspANI GGCC 2 cut(s) 674, 962
BspCNI CTCAG 5 cut(s) 116, 291, 375, 778, 984
BspLI GGNNCC 2 cut(s) 78, 316
BspMI ACCTGC 2 cut(s) 192, 382
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 1 cut(s) 314
BsrDI GCAATG 3 cut(s) 90, 217, 895
BsrFI RCCGGY 3 cut(s) 197, 667, 902
BsrI ACTGG 1 cut(s) 559
BssAI RCCGGY 3 cut(s) 197, 667, 902
BssECI CCNNGG 2 cut(s) 247, 615
BssMI GATC 1 cut(s) 857
BssT1I CCWWGG 2 cut(s) 247, 615
Bst6I CTCTTC 3 cut(s) 140, 166, 537
BstAPI GCANNNNNTGC 2 cut(s) 207, 705
BstC8I GCNNGC 5 cut(s) 222, 334, 389, 625, 669
BstDEI CTNAG 7 cut(s) 103, 278, 383, 714, 765, 780, 992
BstF5I GGATG 3 cut(s) 118, 665, 946
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 1 cut(s) 860
BstMAI GTCTC 2 cut(s) 93, 739
BstMBI GATC 1 cut(s) 857
BstMWI GCNNNNNNNGC 6 cut(s) 207, 323, 620, 705, 781, 845
BstNSI RCATGY 1 cut(s) 912
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 79, 771
BsuRI GGCC 2 cut(s) 674, 962
BtgZI GCGATG 1 cut(s) 741
BtsCI GGATG 3 cut(s) 118, 665, 946
BtsI GCAGTG 2 cut(s) 234, 505
BtsIMutI CAGTG 4 cut(s) 48, 234, 380, 505
BveI ACCTGC 2 cut(s) 192, 382
Cac8I GCNNGC 5 cut(s) 222, 334, 389, 625, 669
CaiI CAGNNNCTG 1 cut(s) 207
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 3 cut(s) 197, 667, 902
CseI GACGC 2 cut(s) 359, 909
Csp6I GTAC 2 cut(s) 400, 769
CviAII CATG 2 cut(s) 577, 909
CviQI GTAC 2 cut(s) 400, 769
DdeI CTNAG 7 cut(s) 103, 278, 383, 714, 765, 780, 992
DpnI GATC 1 cut(s) 859
DpnII GATC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 3 cut(s) 140, 166, 537
EarI CTCTTC 3 cut(s) 140, 166, 537
Eco130I CCWWGG 2 cut(s) 247, 615
Eco57I CTGAAG 2 cut(s) 210, 312
EcoT14I CCWWGG 2 cut(s) 247, 615
ErhI CCWWGG 2 cut(s) 247, 615
FaeI CATG 2 cut(s) 580, 912
FatI CATG 2 cut(s) 576, 908
Fnu4HI GCNGC 5 cut(s) 68, 672, 697, 785, 963
FokI GGATG 3 cut(s) 125, 672, 953
Fsp4HI GCNGC 5 cut(s) 68, 672, 697, 785, 963
GlaI GCGC 1 cut(s) 913
GluI GCNGC 5 cut(s) 68, 672, 697, 785, 963
GsaI CCCAGC 1 cut(s) 495
HaeIII GGCC 2 cut(s) 674, 962
HapII CCGG 4 cut(s) 198, 668, 789, 903
HgaI GACGC 2 cut(s) 359, 909
HhaI GCGC 1 cut(s) 914
Hin1II CATG 2 cut(s) 580, 912
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HindIII AAGCTT 2 cut(s) 218, 716
HinfI GANTC 4 cut(s) 101, 604, 630, 792
HpaII CCGG 4 cut(s) 198, 668, 789, 903
HphI GGTGA 2 cut(s) 71, 941
Hpy166II GTNNAC 1 cut(s) 900
Hpy188I TCNGA 5 cut(s) 229, 513, 635, 925, 958
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 1 cut(s) 900
HpyAV CCTTC 2 cut(s) 837, 963
HpyCH4V TGCA 8 cut(s) 83, 210, 374, 473, 580, 731, 775, 888
HpyF10VI GCNNNNNNNGC 6 cut(s) 207, 323, 620, 705, 781, 845
HpyF3I CTNAG 7 cut(s) 103, 278, 383, 714, 765, 780, 992
Hsp92II CATG 2 cut(s) 580, 912
HspAI GCGC 1 cut(s) 912
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Kzo9I GATC 1 cut(s) 857
LmnI GCTCC 2 cut(s) 495, 620
Lsp1109I GCAGC 2 cut(s) 79, 771
LweI GCATC 2 cut(s) 718, 1011
MaeIII GTNAC 2 cut(s) 12, 637
MalI GATC 1 cut(s) 859
MboI GATC 1 cut(s) 857
MboII GAAGA 7 cut(s) 17, 20, 23, 127, 183, 524, 1006
MhlI GDGCHC 2 cut(s) 48, 625
MluCI AATT 4 cut(s) 36, 375, 432, 972
MlyI GAGTC 2 cut(s) 95, 786
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 7 cut(s) 143, 171, 314, 431, 540, 834, 952
MroNI GCCGGC 1 cut(s) 667
MseI TTAA 6 cut(s) 143, 369, 458, 537, 720, 741
MslI CAYNNNNRTG 1 cut(s) 658
MspA1I CMGCKG 2 cut(s) 282, 332
MspI CCGG 4 cut(s) 198, 668, 789, 903
MspR9I CCNGG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 454
MwoI GCNNNNNNNGC 6 cut(s) 207, 323, 620, 705, 781, 845
NaeI GCCGGC 1 cut(s) 669
NciI CCSGG 1 cut(s) 789
NdeII GATC 1 cut(s) 857
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 2 cut(s) 580, 912
NlaIV GGNNCC 2 cut(s) 78, 316
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 1 cut(s) 912
PaqCI CACCTGC 1 cut(s) 382
PctI GAATGC 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 604, 630
PflMI CCANNNNNTGG 2 cut(s) 394, 656
PkrI GCNGC 5 cut(s) 69, 673, 698, 786, 964
PleI GAGTC 2 cut(s) 95, 786
PpsI GAGTC 2 cut(s) 95, 786
PshBI ATTAAT 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 2 cut(s) 78, 316
PstNI CAGNNNCTG 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 401, 770
RsaNI GTAC 2 cut(s) 400, 769
RseI CAYNNNNRTG 1 cut(s) 658
SaqAI TTAA 6 cut(s) 143, 369, 458, 537, 720, 741
SatI GCNGC 5 cut(s) 68, 672, 697, 785, 963
Sau3AI GATC 1 cut(s) 857
ScaI AGTACT 2 cut(s) 401, 770
SchI GAGTC 2 cut(s) 95, 786
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 2 cut(s) 48, 625
SfaNI GCATC 2 cut(s) 718, 1011
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse9I AATT 4 cut(s) 36, 375, 432, 972
SsiI CCGC 4 cut(s) 282, 671, 696, 963
SspI AATATT 2 cut(s) 571, 684
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 2 cut(s) 247, 615
TasI AATT 4 cut(s) 36, 375, 432, 972
TatI WGTACW 2 cut(s) 399, 768
TauI GCSGC 3 cut(s) 674, 699, 965
TfiI GAWTC 2 cut(s) 604, 630
Tru1I TTAA 6 cut(s) 143, 369, 458, 537, 720, 741
Tru9I TTAA 6 cut(s) 143, 369, 458, 537, 720, 741
TscAI CASTG 4 cut(s) 48, 241, 387, 505
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 2 cut(s) 67, 784
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 3 cut(s) 79, 586, 603
TspRI CASTG 4 cut(s) 48, 241, 387, 505
Van91I CCANNNNNTGG 2 cut(s) 394, 656
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 1 cut(s) 972
XceI RCATGY 1 cut(s) 912
ZrmI AGTACT 2 cut(s) 401, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.