Rh5DG390500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016527
Physical Location & Seq
Forward (+)
60902363 .. 60904231
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG390500.1

Sequence Viewer

Length: 1059 bp
ATGGGTTCTTGTTCTTTGTTACCTTGCTTTCTTCTTCTTCTAATTTCAGTGCTCTATGATGTGGGTGATGGGCAGCCTTTGGTTCCTGCAATGTTCATATTTGGAGACTCAGTTGTGGATGTGGGTAATAACAACAACCTCTTCTCCTTAATCAAATCCAACTTCCCTCCTTATGGAAGAGATTTTGTCAATCACAATCCCACCGGCAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGCTTTGCTTCTTACCAACCAGCTTATCTCAGCGGAGAAGCCACAGGAAAAAGCCTCTTGATTGGTGCCAACTTTGCTTCAGCTGGCTCTGGCTATTATGACGCTACAGCAAAGTTATTTAATGCAATTTCACTGAGCCAGCAGGTGGAGTACTACAAGGAATATCAAAATAAGGTGGTGGGAATTGGAGGAAAAGTGAATGCTACATTAATCATATCGGGTGCAATATACCTTGTTAGTGCTGGGAGCAGTGATTTTGTTCAGAACTACTACATCAATCCTCTTCTTAACAAGGTCTATACGACAGACCAGTTCTCTAATATTCTCATGCAGTCCTATGAAAACTTCATACAGAATCTATATGCCTTGGGAGCACGCAGAATCGGAGTGACCACCCTGCCACCACTTGGATGTCTGCCGGCGGCCATCACAATATTTGGATTAGGCGGCAACAAGTGCGTGGCTAAGCTTAATGGCGATGCAGTCTCATTTAACAATAAGCTAAATGCCACATCTCAGTACTTGCAAACTAAGCTGCCCGGACTCAAGTTGGTCGTCTTTGACATCTACCAGCCTCTCTACAACCTTGTCACGAAGCCTTCAGAGAATGGGTTCTCAGAGGCGAGAAGGGCTTGTTGTGGATCAGGGTTGCTGGAAACATCAATACTATGCAATGCCAAGTCCACCGGCACATGCGCCAATGCGTCAGAGTATGTGTTCTGGGATGGTTTTCACCCTTCGGAGGCCGCAAACAAAATTTTAGCTGATGATTTGCTGAGTAGTGGCATCTCTCTCATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

352

Amino Acids

37.81

Weight (kDa)

5.73

Isoelectric Point (pI)

28.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 342 7.3e-37 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 198, 388
AccB1I GGYRCC 1 cut(s) 320
AccB7I CCANNNNNTGG 2 cut(s) 400, 662
AciI CCGC 4 cut(s) 288, 677, 702, 1002
AclWI GGATC 1 cut(s) 904
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 1011
AcuI CTGAAG 3 cut(s) 216, 318, 840
AfaI GTAC 2 cut(s) 407, 776
AfiI CCNNNNNNNGG 5 cut(s) 173, 207, 400, 662, 997
AluBI AGCT 7 cut(s) 226, 278, 338, 724, 757, 790, 1019
AluI AGCT 7 cut(s) 226, 278, 338, 724, 757, 790, 1019
Alw21I GWGCWC 2 cut(s) 54, 631
Alw26I GTCTC 2 cut(s) 99, 745
AlwI GGATC 1 cut(s) 904
AlwNI CAGNNNCTG 1 cut(s) 213
AoxI GGCC 2 cut(s) 678, 999
ApeKI GCWGC 2 cut(s) 73, 790
ApoI RAATTY 1 cut(s) 1011
AseI ATTAAT 1 cut(s) 464
Asp700I GAANNNNTTC 1 cut(s) 866
AspLEI GCGC 1 cut(s) 953
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 2 cut(s) 77, 980
BanI GGYRCC 1 cut(s) 320
Bbv12I GWGCWC 2 cut(s) 54, 631
BbvI GCAGC 2 cut(s) 85, 777
BccI CCATC 3 cut(s) 62, 689, 974
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 2 cut(s) 99, 745
BfmI CTRYAG 1 cut(s) 360
BfuAI ACCTGC 2 cut(s) 198, 388
BisI GCNGC 5 cut(s) 74, 678, 703, 791, 1002
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 5 cut(s) 75, 679, 704, 792, 1003
BmcAI AGTACT 2 cut(s) 407, 776
Bme1390I CCNGG 1 cut(s) 795
BmiI GGNNCC 2 cut(s) 84, 322
BmrFI CCNGG 1 cut(s) 795
BmsI GCATC 2 cut(s) 724, 1050
Bpu1102I GCTNAGC 1 cut(s) 720
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 795
BsaJI CCNNGG 2 cut(s) 253, 621
BsaXI ACNNNNNCTCC 6 cut(s) 96, 126, 128, 158, 435, 465
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 207, 400, 662, 997
Bse118I RCCGGY 3 cut(s) 203, 673, 941
Bse1I ACTGG 1 cut(s) 565
Bse3DI GCAATG 3 cut(s) 96, 223, 934
BseDI CCNNGG 2 cut(s) 253, 621
BseGI GGATG 3 cut(s) 124, 671, 985
BseLI CCNNNNNNNGG 5 cut(s) 173, 207, 400, 662, 997
BseMI GCAATG 3 cut(s) 96, 223, 934
BseMII CTCAG 6 cut(s) 123, 298, 380, 785, 885, 1022
BseNI ACTGG 1 cut(s) 565
BseXI GCAGC 2 cut(s) 85, 777
BseYI CCCAGC 1 cut(s) 497
BshFI GGCC 2 cut(s) 680, 1001
BshNI GGYRCC 1 cut(s) 320
BsiHKAI GWGCWC 2 cut(s) 54, 631
BsiSI CCGG 4 cut(s) 204, 674, 795, 942
BslI CCNNNNNNNGG 5 cut(s) 173, 207, 400, 662, 997
BsmAI GTCTC 2 cut(s) 99, 745
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 2 cut(s) 680, 1001
Bsp1286I GDGCHC 2 cut(s) 54, 631
Bsp143I GATC 1 cut(s) 896
Bsp1720I GCTNAGC 1 cut(s) 720
BspACI CCGC 4 cut(s) 288, 677, 702, 1002
BspANI GGCC 2 cut(s) 680, 1001
BspCNI CTCAG 6 cut(s) 122, 297, 381, 784, 884, 1023
BspLI GGNNCC 2 cut(s) 84, 322
BspMI ACCTGC 2 cut(s) 198, 388
BspPI GGATC 1 cut(s) 904
BspT107I GGYRCC 1 cut(s) 320
BsrDI GCAATG 3 cut(s) 96, 223, 934
BsrFI RCCGGY 3 cut(s) 203, 673, 941
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 3 cut(s) 203, 673, 941
BssECI CCNNGG 2 cut(s) 253, 621
BssMI GATC 1 cut(s) 896
BssT1I CCWWGG 2 cut(s) 253, 621
Bst6I CTCTTC 3 cut(s) 146, 172, 543
BstAPI GCANNNNNTGC 2 cut(s) 213, 711
BstC8I GCNNGC 5 cut(s) 228, 340, 395, 631, 675
BstDEI CTNAG 8 cut(s) 109, 284, 389, 720, 771, 786, 871, 1031
BstF5I GGATG 3 cut(s) 124, 671, 985
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 1 cut(s) 899
BstMAI GTCTC 2 cut(s) 99, 745
BstMBI GATC 1 cut(s) 896
BstMWI GCNNNNNNNGC 6 cut(s) 213, 329, 626, 711, 787, 884
BstNSI RCATGY 1 cut(s) 951
BstSCI CCNGG 1 cut(s) 793
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 85, 777
BsuRI GGCC 2 cut(s) 680, 1001
BtgZI GCGATG 1 cut(s) 747
BtsCI GGATG 3 cut(s) 124, 671, 985
BtsI GCAGTG 2 cut(s) 240, 511
BtsIMutI CAGTG 4 cut(s) 54, 240, 386, 511
BveI ACCTGC 2 cut(s) 198, 388
Cac8I GCNNGC 5 cut(s) 228, 340, 395, 631, 675
CaiI CAGNNNCTG 1 cut(s) 213
CfoI GCGC 1 cut(s) 953
Cfr10I RCCGGY 3 cut(s) 203, 673, 941
CseI GACGC 2 cut(s) 365, 948
Csp6I GTAC 2 cut(s) 406, 775
CviAII CATG 2 cut(s) 583, 948
CviQI GTAC 2 cut(s) 406, 775
DdeI CTNAG 8 cut(s) 109, 284, 389, 720, 771, 786, 871, 1031
DpnI GATC 1 cut(s) 898
DpnII GATC 1 cut(s) 896
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 3 cut(s) 146, 172, 543
EarI CTCTTC 3 cut(s) 146, 172, 543
Eco130I CCWWGG 2 cut(s) 253, 621
Eco57I CTGAAG 3 cut(s) 216, 318, 840
EcoT14I CCWWGG 2 cut(s) 253, 621
ErhI CCWWGG 2 cut(s) 253, 621
FaeI CATG 2 cut(s) 586, 951
FatI CATG 2 cut(s) 582, 947
Fnu4HI GCNGC 5 cut(s) 74, 678, 703, 791, 1002
FokI GGATG 3 cut(s) 131, 678, 992
Fsp4HI GCNGC 5 cut(s) 74, 678, 703, 791, 1002
GlaI GCGC 1 cut(s) 952
GluI GCNGC 5 cut(s) 74, 678, 703, 791, 1002
GsaI CCCAGC 1 cut(s) 501
HaeIII GGCC 2 cut(s) 680, 1001
HapII CCGG 4 cut(s) 204, 674, 795, 942
HgaI GACGC 2 cut(s) 365, 948
HhaI GCGC 1 cut(s) 953
Hin1II CATG 2 cut(s) 586, 951
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HindIII AAGCTT 2 cut(s) 224, 722
HinfI GANTC 4 cut(s) 107, 610, 636, 798
HpaII CCGG 4 cut(s) 204, 674, 795, 942
HphI GGTGA 2 cut(s) 77, 980
Hpy166II GTNNAC 1 cut(s) 939
Hpy188I TCNGA 7 cut(s) 235, 519, 641, 859, 874, 964, 997
Hpy188III TCNNGA 2 cut(s) 313, 847
Hpy8I GTNNAC 1 cut(s) 939
HpyAV CCTTC 3 cut(s) 864, 876, 1002
HpyCH4V TGCA 8 cut(s) 89, 216, 380, 479, 586, 737, 781, 927
HpyF10VI GCNNNNNNNGC 6 cut(s) 213, 329, 626, 711, 787, 884
HpyF3I CTNAG 8 cut(s) 109, 284, 389, 720, 771, 786, 871, 1031
Hsp92II CATG 2 cut(s) 586, 951
HspAI GCGC 1 cut(s) 951
KroI GCCGGC 1 cut(s) 673
KroNI GCCGGC 1 cut(s) 675
Kzo9I GATC 1 cut(s) 896
LmnI GCTCC 2 cut(s) 501, 626
Lsp1109I GCAGC 2 cut(s) 85, 777
LweI GCATC 2 cut(s) 724, 1050
MaeIII GTNAC 3 cut(s) 18, 643, 844
MalI GATC 1 cut(s) 898
MboI GATC 1 cut(s) 896
MboII GAAGA 6 cut(s) 23, 26, 29, 133, 189, 530
MhlI GDGCHC 2 cut(s) 54, 631
MluCI AATT 4 cut(s) 42, 381, 438, 1011
MlyI GAGTC 2 cut(s) 101, 792
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 8 cut(s) 149, 177, 320, 437, 546, 840, 868, 991
MroNI GCCGGC 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 866
MseI TTAA 7 cut(s) 149, 375, 464, 543, 726, 747, 1057
MslI CAYNNNNRTG 1 cut(s) 664
MspA1I CMGCKG 2 cut(s) 288, 338
MspI CCGG 4 cut(s) 204, 674, 795, 942
MspR9I CCNGG 1 cut(s) 795
Mva1269I GAATGC 1 cut(s) 460
MwoI GCNNNNNNNGC 6 cut(s) 213, 329, 626, 711, 787, 884
NaeI GCCGGC 1 cut(s) 675
NciI CCSGG 1 cut(s) 795
NdeII GATC 1 cut(s) 896
NgoMIV GCCGGC 1 cut(s) 673
NlaIII CATG 2 cut(s) 586, 951
NlaIV GGNNCC 2 cut(s) 84, 322
NmuCI GTSAC 2 cut(s) 643, 844
NspI RCATGY 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 388
PctI GAATGC 1 cut(s) 460
PdiI GCCGGC 1 cut(s) 675
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 2 cut(s) 610, 636
PflMI CCANNNNNTGG 2 cut(s) 400, 662
PkrI GCNGC 5 cut(s) 75, 679, 704, 792, 1003
PleI GAGTC 2 cut(s) 101, 792
PpsI GAGTC 2 cut(s) 101, 792
PshBI ATTAAT 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 497
PspN4I GGNNCC 2 cut(s) 84, 322
PstNI CAGNNNCTG 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 2 cut(s) 407, 776
RsaNI GTAC 2 cut(s) 406, 775
RseI CAYNNNNRTG 1 cut(s) 664
SaqAI TTAA 7 cut(s) 149, 375, 464, 543, 726, 747, 1057
SatI GCNGC 5 cut(s) 74, 678, 703, 791, 1002
Sau3AI GATC 1 cut(s) 896
ScaI AGTACT 2 cut(s) 407, 776
SchI GAGTC 2 cut(s) 101, 792
ScrFI CCNGG 1 cut(s) 795
SduI GDGCHC 2 cut(s) 54, 631
SfaNI GCATC 2 cut(s) 724, 1050
SfcI CTRYAG 1 cut(s) 360
SmiMI CAYNNNNRTG 1 cut(s) 664
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 4 cut(s) 42, 381, 438, 1011
SsiI CCGC 4 cut(s) 288, 677, 702, 1002
SspI AATATT 2 cut(s) 577, 690
StyD4I CCNGG 1 cut(s) 793
StyI CCWWGG 2 cut(s) 253, 621
TasI AATT 4 cut(s) 42, 381, 438, 1011
TatI WGTACW 2 cut(s) 405, 774
TauI GCSGC 3 cut(s) 680, 705, 1004
TfiI GAWTC 2 cut(s) 610, 636
Tru1I TTAA 7 cut(s) 149, 375, 464, 543, 726, 747, 1057
Tru9I TTAA 7 cut(s) 149, 375, 464, 543, 726, 747, 1057
TscAI CASTG 4 cut(s) 54, 247, 393, 511
TseFI GTSAC 2 cut(s) 643, 844
TseI GCWGC 2 cut(s) 73, 790
Tsp45I GTSAC 2 cut(s) 643, 844
TspDTI ATGAA 3 cut(s) 85, 592, 609
TspRI CASTG 4 cut(s) 54, 247, 393, 511
Van91I CCANNNNNTGG 2 cut(s) 400, 662
VspI ATTAAT 1 cut(s) 464
XapI RAATTY 1 cut(s) 1011
XceI RCATGY 1 cut(s) 951
XmnI GAANNNNTTC 1 cut(s) 866
ZrmI AGTACT 2 cut(s) 407, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.