Rroxscaffold_1G00024440

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016527
Physical Location & Seq
Reverse (-)
30340177 .. 30342090
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024440.1

Sequence Viewer

Length: 1062 bp
ATGGGTTCTTTGTGTTCTTGGTTGCCTTGCTTTTTTCTTCTTCTAATTTCAGTGCTCTGTGATGTGGGTGATGGGCAGCCTTTGGTTCCTGCAATTTTCATATTTGGAGACTCAGTTGTGGATGTGGGTAATAACAACAACCTCTTCTCCTTAATCAAATCCAACTTCCCTCCTTATGGAAGAGACTTTGTCAATCACAATCCCACCGGCAGGTTCTGCAATGGAAAGCTTGCTTCAGACTTCACTGCTGAAAACCTTGGCTTCGCTTCTCACCAACCAGCTTATCTCAGCGGAGAAGCCACAGGGAAAAGCCTCTTGATTGGTGCCAACTTTGCTTCAGCTGGCTCTGGCTATTATGACGCTACAGCAAAGTTATTTAATGCAATTTCACTGAGCCAGCAGGTAGAGTACTACAAGGAATATCAAAATAAGGTGGTGGGAATTGGAGGAAAAGTGAATGCTACATTAATCATATCGGGTGCAATATACCTTGTTAGTGCTGGGAGCAGTGATTTCGTTCAGAACTACTACATCAATCCTCTTCTTAACAAGGTCTATACGACAGACCAGTTCTCTAACATTCTCATGCAGTCCTATGAACACTTCATACAGAATCTATATGCCTTGGGAGCACGCAGAATCGGAGTGACCACCCTGCCACCACTTGGATGTCTGCCGGCGGCCATCACAATATTTGGATTAGGCGGCAACAAGTGCGTGGCTAAGCTTAATGGCGATGCAGTCTCATTTAACGACAAGCTAAATGCCACATCTCAGTACTTGCAAACTAAGCTGCCCGGACTCAAGTTGGTCGTCTTTGACATCTACCAGCCTCTCTACAACCTTGTCACGAAGCCTTCAGAGAATGGGTTCTCAGAGGCGAGAAGGGCTTGTTGTGGATCAGGGTTGCTGGAAACATCAATACTATGCAATGCCAAGTCCATCGGCACATGCGCCAATGCGTCGGAGTACGTGTTCTGGGATGGGTTTCATCCTTCGGAGGCCGCAAACAAAATTTTAGCTGATGATTTGCTGAGTAGTGGCATCTCTCTCATCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

353

Amino Acids

37.97

Weight (kDa)

5.81

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 343 7.4e-37 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22810
fragaria_vesca FvH4_3g30030
malus_domestica MD11G1153800.v1.1
prunus_persica Prupe.6G116500_v2.0.a1 Prupe.6G116500_v2.0.a1
pyrus_communis pycom11g12550
rosa_chinensis RchiOBHm_Chr5g0055911
rosa_laevigata RLG00000035021
rosa_multiflora Rmu_sc0016879.1_g000001
rosa_roxburghii Rroxscaffold_1G00024440
rosa_samantha Rh5BG377000 Rh5CG400300 Rh5DG390500
rosa_wichuraiana Rw5G034390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 201, 391
AccB1I GGYRCC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 665
AciI CCGC 4 cut(s) 291, 680, 705, 1005
AclWI GGATC 1 cut(s) 907
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 1 cut(s) 1014
AcuI CTGAAG 3 cut(s) 219, 321, 843
AfaI GTAC 3 cut(s) 410, 779, 971
AfiI CCNNNNNNNGG 3 cut(s) 176, 210, 665
AflIII ACRYGT 1 cut(s) 972
AloI GAACNNNNNNTCC 2 cut(s) 959, 991
AluBI AGCT 7 cut(s) 229, 281, 341, 727, 760, 793, 1022
AluI AGCT 7 cut(s) 229, 281, 341, 727, 760, 793, 1022
Alw21I GWGCWC 2 cut(s) 57, 634
Alw26I GTCTC 3 cut(s) 102, 177, 748
AlwI GGATC 1 cut(s) 907
AlwNI CAGNNNCTG 1 cut(s) 216
AoxI GGCC 2 cut(s) 681, 1002
ApeKI GCWGC 2 cut(s) 76, 793
ApoI RAATTY 1 cut(s) 1014
AseI ATTAAT 1 cut(s) 467
Asp700I GAANNNNTTC 1 cut(s) 869
AspLEI GCGC 1 cut(s) 956
AsuC2I CCSGG 1 cut(s) 798
AsuHPI GGTGA 2 cut(s) 80, 263
BanI GGYRCC 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 57, 634
BbvI GCAGC 2 cut(s) 88, 780
BccI CCATC 4 cut(s) 65, 692, 950, 977
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BcnI CCSGG 1 cut(s) 798
BcoDI GTCTC 3 cut(s) 102, 177, 748
BfaI CTAG 1 cut(s) 1060
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 2 cut(s) 201, 391
BisI GCNGC 5 cut(s) 77, 681, 706, 794, 1005
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 5 cut(s) 78, 682, 707, 795, 1006
BmcAI AGTACT 2 cut(s) 410, 779
Bme1390I CCNGG 1 cut(s) 798
BmiI GGNNCC 2 cut(s) 87, 325
BmrFI CCNGG 1 cut(s) 798
BmsI GCATC 2 cut(s) 727, 1053
Bpu1102I GCTNAGC 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 973
BsaJI CCNNGG 2 cut(s) 256, 624
BsaXI ACNNNNNCTCC 8 cut(s) 99, 129, 131, 161, 438, 468, 959, 989
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 210, 665
Bse118I RCCGGY 2 cut(s) 206, 676
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 2 cut(s) 226, 937
BseDI CCNNGG 2 cut(s) 256, 624
BseGI GGATG 4 cut(s) 127, 674, 988, 991
BseLI CCNNNNNNNGG 3 cut(s) 176, 210, 665
BseMI GCAATG 2 cut(s) 226, 937
BseMII CTCAG 6 cut(s) 126, 301, 383, 788, 888, 1025
BseNI ACTGG 1 cut(s) 568
BseXI GCAGC 2 cut(s) 88, 780
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 2 cut(s) 683, 1004
BshNI GGYRCC 1 cut(s) 323
BsiHKAI GWGCWC 2 cut(s) 57, 634
BsiSI CCGG 3 cut(s) 207, 677, 798
BslI CCNNNNNNNGG 3 cut(s) 176, 210, 665
BsmAI GTCTC 3 cut(s) 102, 177, 748
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 2 cut(s) 683, 1004
Bsp1286I GDGCHC 2 cut(s) 57, 634
Bsp143I GATC 1 cut(s) 899
Bsp1720I GCTNAGC 1 cut(s) 723
BspACI CCGC 4 cut(s) 291, 680, 705, 1005
BspANI GGCC 2 cut(s) 683, 1004
BspCNI CTCAG 6 cut(s) 125, 300, 384, 787, 887, 1026
BspLI GGNNCC 2 cut(s) 87, 325
BspMI ACCTGC 2 cut(s) 201, 391
BspPI GGATC 1 cut(s) 907
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 226, 937
BsrFI RCCGGY 2 cut(s) 206, 676
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 2 cut(s) 206, 676
BssECI CCNNGG 2 cut(s) 256, 624
BssMI GATC 1 cut(s) 899
BssT1I CCWWGG 2 cut(s) 256, 624
Bst6I CTCTTC 3 cut(s) 149, 175, 546
BstAPI GCANNNNNTGC 2 cut(s) 216, 714
BstBAI YACGTR 1 cut(s) 973
BstC8I GCNNGC 5 cut(s) 231, 343, 398, 634, 678
BstDEI CTNAG 8 cut(s) 112, 287, 392, 723, 774, 789, 874, 1034
BstF5I GGATG 4 cut(s) 127, 674, 988, 991
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 1 cut(s) 902
BstMAI GTCTC 3 cut(s) 102, 177, 748
BstMBI GATC 1 cut(s) 899
BstMWI GCNNNNNNNGC 6 cut(s) 216, 332, 629, 714, 790, 887
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 1 cut(s) 796
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 88, 780
BsuRI GGCC 2 cut(s) 683, 1004
BtgZI GCGATG 1 cut(s) 750
BtsCI GGATG 4 cut(s) 127, 674, 988, 991
BtsI GCAGTG 2 cut(s) 243, 514
BtsIMutI CAGTG 4 cut(s) 57, 243, 389, 514
BveI ACCTGC 2 cut(s) 201, 391
Cac8I GCNNGC 5 cut(s) 231, 343, 398, 634, 678
CaiI CAGNNNCTG 1 cut(s) 216
CfoI GCGC 1 cut(s) 956
Cfr10I RCCGGY 2 cut(s) 206, 676
CseI GACGC 2 cut(s) 368, 951
Csp6I GTAC 3 cut(s) 409, 778, 970
CviAII CATG 2 cut(s) 586, 951
CviQI GTAC 3 cut(s) 409, 778, 970
DdeI CTNAG 8 cut(s) 112, 287, 392, 723, 774, 789, 874, 1034
DpnI GATC 1 cut(s) 901
DpnII GATC 1 cut(s) 899
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 3 cut(s) 149, 175, 546
EarI CTCTTC 3 cut(s) 149, 175, 546
Eco130I CCWWGG 2 cut(s) 256, 624
Eco57I CTGAAG 3 cut(s) 219, 321, 843
EcoT14I CCWWGG 2 cut(s) 256, 624
ErhI CCWWGG 2 cut(s) 256, 624
FaeI CATG 2 cut(s) 589, 954
FatI CATG 2 cut(s) 585, 950
Fnu4HI GCNGC 5 cut(s) 77, 681, 706, 794, 1005
FokI GGATG 4 cut(s) 134, 681, 978, 995
Fsp4HI GCNGC 5 cut(s) 77, 681, 706, 794, 1005
FspBI CTAG 1 cut(s) 1060
GlaI GCGC 1 cut(s) 955
GluI GCNGC 5 cut(s) 77, 681, 706, 794, 1005
GsaI CCCAGC 1 cut(s) 504
HaeIII GGCC 2 cut(s) 683, 1004
HapII CCGG 3 cut(s) 207, 677, 798
HgaI GACGC 2 cut(s) 368, 951
HhaI GCGC 1 cut(s) 956
Hin1II CATG 2 cut(s) 589, 954
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HindIII AAGCTT 2 cut(s) 227, 725
HinfI GANTC 4 cut(s) 110, 613, 639, 801
HpaII CCGG 3 cut(s) 207, 677, 798
HphI GGTGA 2 cut(s) 80, 263
Hpy188I TCNGA 7 cut(s) 238, 522, 644, 862, 877, 967, 1000
Hpy188III TCNNGA 2 cut(s) 316, 850
Hpy99I CGWCG 1 cut(s) 967
HpyAV CCTTC 3 cut(s) 867, 879, 1005
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 8 cut(s) 92, 219, 383, 482, 589, 740, 784, 930
HpyF10VI GCNNNNNNNGC 6 cut(s) 216, 332, 629, 714, 790, 887
HpyF3I CTNAG 8 cut(s) 112, 287, 392, 723, 774, 789, 874, 1034
HpySE526I ACGT 1 cut(s) 972
Hsp92II CATG 2 cut(s) 589, 954
HspAI GCGC 1 cut(s) 954
KroI GCCGGC 1 cut(s) 676
KroNI GCCGGC 1 cut(s) 678
Kzo9I GATC 1 cut(s) 899
LmnI GCTCC 2 cut(s) 504, 629
Lsp1109I GCAGC 2 cut(s) 88, 780
LweI GCATC 2 cut(s) 727, 1053
MaeI CTAG 1 cut(s) 1060
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 2 cut(s) 646, 847
MalI GATC 1 cut(s) 901
MboI GATC 1 cut(s) 899
MboII GAAGA 6 cut(s) 29, 32, 136, 192, 533, 1048
MhlI GDGCHC 2 cut(s) 57, 634
MluCI AATT 5 cut(s) 45, 93, 384, 441, 1014
MlyI GAGTC 2 cut(s) 104, 795
MmeI TCCRAC 2 cut(s) 186, 945
MnlI CCTC 8 cut(s) 152, 180, 323, 440, 549, 843, 871, 994
MroNI GCCGGC 1 cut(s) 676
MroXI GAANNNNTTC 1 cut(s) 869
MseI TTAA 6 cut(s) 152, 378, 467, 546, 729, 750
MslI CAYNNNNRTG 2 cut(s) 584, 667
MspA1I CMGCKG 2 cut(s) 291, 341
MspI CCGG 3 cut(s) 207, 677, 798
MspR9I CCNGG 1 cut(s) 798
Mva1269I GAATGC 1 cut(s) 463
MwoI GCNNNNNNNGC 6 cut(s) 216, 332, 629, 714, 790, 887
NaeI GCCGGC 1 cut(s) 678
NciI CCSGG 1 cut(s) 798
NdeII GATC 1 cut(s) 899
NgoMIV GCCGGC 1 cut(s) 676
NlaIII CATG 2 cut(s) 589, 954
NlaIV GGNNCC 2 cut(s) 87, 325
NmuCI GTSAC 2 cut(s) 646, 847
NspI RCATGY 1 cut(s) 954
PctI GAATGC 1 cut(s) 463
PdiI GCCGGC 1 cut(s) 678
PdmI GAANNNNTTC 1 cut(s) 869
PfeI GAWTC 2 cut(s) 613, 639
PflFI GACNNNGTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 665
PkrI GCNGC 5 cut(s) 78, 682, 707, 795, 1006
PleI GAGTC 2 cut(s) 104, 795
PpsI GAGTC 2 cut(s) 104, 795
Ppu21I YACGTR 1 cut(s) 973
PshBI ATTAAT 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 500
PspN4I GGNNCC 2 cut(s) 87, 325
PsrI GAACNNNNNNTAC 2 cut(s) 591, 623
PstNI CAGNNNCTG 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 3 cut(s) 410, 779, 971
RsaNI GTAC 3 cut(s) 409, 778, 970
RseI CAYNNNNRTG 2 cut(s) 584, 667
SaqAI TTAA 6 cut(s) 152, 378, 467, 546, 729, 750
SatI GCNGC 5 cut(s) 77, 681, 706, 794, 1005
Sau3AI GATC 1 cut(s) 899
ScaI AGTACT 2 cut(s) 410, 779
SchI GAGTC 2 cut(s) 104, 795
ScrFI CCNGG 1 cut(s) 798
SduI GDGCHC 2 cut(s) 57, 634
SfaNI GCATC 2 cut(s) 727, 1053
SfcI CTRYAG 1 cut(s) 363
SmiMI CAYNNNNRTG 2 cut(s) 584, 667
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 5 cut(s) 45, 93, 384, 441, 1014
SsiI CCGC 4 cut(s) 291, 680, 705, 1005
SspI AATATT 1 cut(s) 693
SspMI CTAG 1 cut(s) 1060
StyD4I CCNGG 1 cut(s) 796
StyI CCWWGG 2 cut(s) 256, 624
TaiI ACGT 1 cut(s) 975
TasI AATT 5 cut(s) 45, 93, 384, 441, 1014
TatI WGTACW 2 cut(s) 408, 777
TauI GCSGC 3 cut(s) 683, 708, 1007
TfiI GAWTC 2 cut(s) 613, 639
Tru1I TTAA 6 cut(s) 152, 378, 467, 546, 729, 750
Tru9I TTAA 6 cut(s) 152, 378, 467, 546, 729, 750
TscAI CASTG 4 cut(s) 57, 250, 396, 514
TseFI GTSAC 2 cut(s) 646, 847
TseI GCWGC 2 cut(s) 76, 793
Tsp45I GTSAC 2 cut(s) 646, 847
TspDTI ATGAA 4 cut(s) 88, 595, 612, 980
TspRI CASTG 4 cut(s) 57, 250, 396, 514
Tth111I GACNNNGTC 1 cut(s) 188
Van91I CCANNNNNTGG 1 cut(s) 665
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 1 cut(s) 1014
XceI RCATGY 1 cut(s) 954
XmnI GAANNNNTTC 1 cut(s) 869
XspI CTAG 1 cut(s) 1060
ZrmI AGTACT 2 cut(s) 410, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.