MD11G1267300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004081
Physical Location & Seq
Forward (+)
38241191 .. 38246837
5647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1267300.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGTCGGTCGAGAGCCCCTTCGAAGCATGGGAGGAGGTACAGCGCCATGGGCAGGACTTGGCGGACCGCCTCACGCAGGGCTTCACGGGTTTAATTCAGTCCCACATCACTCCGCCGTCGTTTCATTGGCCAAACCCTAACACATCGAAGCTCTTCGACCTCGAATTCCCGAACCAGAGTTTTGGCAAGAGGGATTTTGTTGGCTTGGTGGCTGAGAACCCGGGCATTAACGGGGTTTCTGCGATTCTTGATATCGGTAACAGGATTGGGCAAGCCGGGGCGGATTTTGGGGCGTGCTTGAATGGGATGCTTCAGCAGTTCTTTAGGGCCTTACCCGTGCCGTTTAGGCATGATGAGAATGTTGATTTGGCGGCAATTCGGATTGATTCGGGTAAGGCTAAGCACAGAGCTGATATGATTTTGAGTGGCAAGGAGGATTTGGGGCCGTTGAAGGAACGGTTGAAGGATTTCAGGGTTGTGGAGAGTGATGGTGGCTCTGATGGTGTGGTGGATGAGGAACTTGGTGGCTTGAATCTAAGGTCTGCTGGTCTTCTGGGTAGACCACTGGGGACTGTAAATACCACAACAAGTTATGATACTAGAACCCGTAAGCTGGAAAGTTCTTTCCTTGCAAAGGGAGACTTATGGAGAGTAGAGGCGTCTAGTGGCAGTTCCACATCTAGAAATGACAATTCATCACTTTTCCTTGTCCAGCTTGGGCCGTTACTCTTTGTTCGTGATGCAACTCTTCTTCTTCCAGTGCATTTGTCAAAGCAACACCTGCTTTGGTATGGCTATGATAGGAAGAATAGGGTGCATTCTCTTTGTCCGGCTGTGTGGTCAAAACACAGAAGGTGGCTCTTAATGTCAATGGTCTGCCTGAAACCATTAACTTGTTCGTTTGTTGATTTACAGTTTCCAAACGGGCAGTTTACATATATATCTGGTGAAGGACTTAGTACAAGTGCTTTCTTACCTCTTTGTGGAGGCCTTCTACAAGCTCAAGGTCAATATCCTGGAGAAATGATATTCAGTTTCTCTCGCAAGAATAAGTGGGGAACACGCATCACACCCATGATACAATGGCCAGATAAGTCTTTTACACTGGGTTTTTCGCAAGCCTTGGCTTGGCAGCGATCTGGTCTTATGGTGAAGCCCACAATTCAGTTCAGTTTATGTCCCACTATTGGTGGAAGCAATCCTGGTTTGCAGGCAGAGCTCATTCACTCAGTGAAGGAGCAGCTGAATCTTATATGTGGTGGTGCATTTACAGCACATCCTTCTGCTTTTGCTTCAATATCGGTTGGCAGATCCAAATGGAATGGAAATGTTGGGGACTCGGGGATCGTTTTAAGAGTGGATACTCCTCTTTCCAATGTTGGTCGACCATACTTCTCGATTCAGTTAAATAATGTTCTTGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

476

Amino Acids

52.26

Weight (kDa)

8.41

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 789
AccI GTMKAC 2 cut(s) 559, 1384
AciI CCGC 5 cut(s) 62, 67, 113, 281, 371
AclWI GGATC 2 cut(s) 1305, 1352
AcoI YGGCCR 2 cut(s) 128, 1085
AcsI RAATTY 2 cut(s) 164, 1421
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 659
AdeI CACNNNGTG 1 cut(s) 1231
AfaI GTAC 2 cut(s) 39, 961
AfiI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 983, 1128, 1187
AgsI TTSAA 6 cut(s) 301, 451, 463, 532, 1296, 1421
AjnI CCWGG 2 cut(s) 1015, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 7 cut(s) 151, 410, 613, 715, 1001, 1219, 1243
AluI AGCT 7 cut(s) 151, 410, 613, 715, 1001, 1219, 1243
Alw21I GWGCWC 1 cut(s) 1221
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 2 cut(s) 1305, 1352
Ama87I CYCGRG 2 cut(s) 220, 1339
AoxI GGCC 6 cut(s) 128, 327, 443, 719, 988, 1085
ApeKI GCWGC 2 cut(s) 1132, 1240
ApoI RAATTY 2 cut(s) 164, 1421
Asp700I GAANNNNTTC 2 cut(s) 152, 467
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 4 cut(s) 64, 327, 443, 719
AsuC2I CCSGG 3 cut(s) 221, 222, 277
AsuHPI GGTGA 2 cut(s) 959, 1162
AsuII TTCGAA 1 cut(s) 21
AvaI CYCGRG 2 cut(s) 220, 1339
AvaII GGWCC 1 cut(s) 64
BalI TGGCCA 2 cut(s) 130, 1087
BanII GRGCYC 2 cut(s) 17, 1221
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 1221
BbvI GCAGC 2 cut(s) 1144, 1252
BccI CCATC 2 cut(s) 482, 494
BceAI ACGGC 4 cut(s) 100, 325, 430, 706
BciT130I CCWGG 2 cut(s) 1017, 1203
BciVI GTATCC 1 cut(s) 1354
BcnI CCSGG 3 cut(s) 221, 222, 277
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 3 cut(s) 600, 663, 681
BfoI RGCGCY 1 cut(s) 46
BfuAI ACCTGC 1 cut(s) 789
BfuI GTATCC 1 cut(s) 1354
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 372, 1133, 1241
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 3 cut(s) 373, 1134, 1242
Bme1390I CCNGG 5 cut(s) 221, 222, 277, 1017, 1203
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 220, 1339
BmgT120I GGNCC 4 cut(s) 64, 327, 443, 719
BmiI GGNNCC 1 cut(s) 444
BmrFI CCNGG 5 cut(s) 221, 222, 277, 1017, 1203
BmrI ACTGGG 2 cut(s) 575, 1115
BmsI GCATC 3 cut(s) 297, 730, 1074
BmuI ACTGGG 2 cut(s) 575, 1115
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 1038
Bpu1102I GCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 987
BpuMI CCSGG 3 cut(s) 221, 222, 277
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 4 cut(s) 46, 220, 276, 1122
Bsc4I CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 983, 1128, 1187
Bse1I ACTGG 3 cut(s) 570, 758, 1110
BseBI CCWGG 2 cut(s) 1017, 1203
BseDI CCNNGG 4 cut(s) 46, 220, 276, 1122
BseGI GGATG 3 cut(s) 312, 517, 1276
BseLI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 983, 1128, 1187
BseMII CTCAG 2 cut(s) 204, 1242
BseNI ACTGG 3 cut(s) 570, 758, 1110
BseRI GAGGAG 2 cut(s) 47, 1356
BseXI GCAGC 2 cut(s) 1144, 1252
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 6 cut(s) 130, 329, 445, 721, 990, 1087
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 1221
BsiHKCI CYCGRG 2 cut(s) 220, 1339
BsiSI CCGG 3 cut(s) 221, 276, 830
BslFI GGGAC 4 cut(s) 85, 583, 1164, 1349
BslI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 983, 1128, 1187
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 4 cut(s) 85, 583, 1164, 1349
BsmI GAATGC 1 cut(s) 817
BsnI GGCC 6 cut(s) 130, 329, 445, 721, 990, 1087
BsoBI CYCGRG 2 cut(s) 220, 1339
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 2 cut(s) 17, 1221
Bsp143I GATC 3 cut(s) 1136, 1310, 1344
Bsp1720I GCTNAGC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 5 cut(s) 62, 67, 113, 281, 371
BspANI GGCC 6 cut(s) 130, 329, 445, 721, 990, 1087
BspCNI CTCAG 2 cut(s) 205, 1241
BspLI GGNNCC 1 cut(s) 444
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 2 cut(s) 1305, 1352
BspQI GCTCTTC 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 21
BsrI ACTGG 3 cut(s) 570, 758, 1110
BssECI CCNNGG 4 cut(s) 46, 220, 276, 1122
BssMI GATC 3 cut(s) 1136, 1310, 1344
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 2 cut(s) 46, 1122
Bst2UI CCWGG 2 cut(s) 1017, 1203
Bst4CI ACNGT 3 cut(s) 459, 574, 915
Bst6I CTCTTC 2 cut(s) 158, 753
BstACI GRCGYC 1 cut(s) 659
BstAPI GCANNNNNTGC 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 4 cut(s) 273, 295, 1119, 1212
BstDEI CTNAG 5 cut(s) 213, 399, 536, 956, 1228
BstDSI CCRYGG 1 cut(s) 46
BstENI CCTNNNNNAGG 2 cut(s) 74, 632
BstF5I GGATG 3 cut(s) 312, 517, 1276
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 3 cut(s) 1139, 1313, 1347
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 3 cut(s) 1136, 1310, 1344
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 5 cut(s) 49, 346, 781, 1216, 1271
BstNI CCWGG 2 cut(s) 1017, 1203
BstSCI CCNGG 5 cut(s) 219, 220, 275, 1015, 1201
BstV1I GCAGC 2 cut(s) 1144, 1252
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 1310
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 1354
BsuRI GGCC 6 cut(s) 130, 329, 445, 721, 990, 1087
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 3 cut(s) 312, 517, 1276
BtsIMutI CAGTG 4 cut(s) 563, 765, 1103, 1236
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 4 cut(s) 273, 295, 1119, 1212
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 64, 327, 443, 719
Cfr9I CCCGGG 1 cut(s) 220
CpoI CGGWCCG 1 cut(s) 64
CseI GACGC 1 cut(s) 648
Csp6I GTAC 2 cut(s) 38, 960
CspI CGGWCCG 1 cut(s) 64
CviAII CATG 4 cut(s) 27, 47, 350, 1075
CviQI GTAC 2 cut(s) 38, 960
DdeI CTNAG 5 cut(s) 213, 399, 536, 956, 1228
DpnI GATC 3 cut(s) 1138, 1312, 1346
DpnII GATC 3 cut(s) 1136, 1310, 1344
DraIII CACNNNGTG 1 cut(s) 1231
EaeI YGGCCR 2 cut(s) 128, 1085
Eam1104I CTCTTC 2 cut(s) 158, 753
EarI CTCTTC 2 cut(s) 158, 753
EciI GGCGGA 3 cut(s) 77, 102, 296
Ecl136II GAGCTC 1 cut(s) 1219
Eco130I CCWWGG 2 cut(s) 46, 1122
Eco147I AGGCCT 1 cut(s) 990
Eco24I GRGCYC 2 cut(s) 17, 1221
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 1219
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 2 cut(s) 220, 1339
EcoICRI GAGCTC 1 cut(s) 1219
EcoNI CCTNNNNNAGG 2 cut(s) 74, 632
EcoO109I RGGNCCY 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 1015, 1201
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 46, 1122
EcoT38I GRGCYC 2 cut(s) 17, 1221
ErhI CCWWGG 2 cut(s) 46, 1122
FaeI CATG 4 cut(s) 30, 50, 353, 1078
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 4 cut(s) 85, 583, 1164, 1349
FatI CATG 4 cut(s) 26, 46, 349, 1074
FblI GTMKAC 2 cut(s) 559, 1384
Fnu4HI GCNGC 3 cut(s) 372, 1133, 1241
FokI GGATG 3 cut(s) 319, 524, 1263
FriOI GRGCYC 2 cut(s) 17, 1221
Fsp4HI GCNGC 3 cut(s) 372, 1133, 1241
FspBI CTAG 3 cut(s) 600, 663, 681
GlaI GCGC 1 cut(s) 44
GluI GCNGC 3 cut(s) 372, 1133, 1241
GsuI CTGGAG 1 cut(s) 1038
HaeII RGCGCY 1 cut(s) 46
HaeIII GGCC 6 cut(s) 130, 329, 445, 721, 990, 1087
HapII CCGG 3 cut(s) 221, 276, 830
HgaI GACGC 1 cut(s) 648
HhaI GCGC 1 cut(s) 45
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 4 cut(s) 30, 50, 353, 1078
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HincII GTYRAC 1 cut(s) 1385
HindII GTYRAC 1 cut(s) 1385
HinfI GANTC 6 cut(s) 244, 386, 532, 1246, 1337, 1399
HpaII CCGG 3 cut(s) 221, 276, 830
HphI GGTGA 2 cut(s) 959, 1162
Hpy166II GTNNAC 3 cut(s) 560, 933, 1385
Hpy188I TCNGA 2 cut(s) 381, 499
Hpy188III TCNNGA 7 cut(s) 10, 169, 248, 681, 737, 1396, 1418
Hpy8I GTNNAC 3 cut(s) 560, 933, 1385
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 8 cut(s) 28, 445, 457, 846, 944, 1001, 1228, 1290
HpyCH4III ACNGT 3 cut(s) 459, 574, 915
HpyCH4V TGCA 6 cut(s) 632, 743, 763, 817, 1210, 1265
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 346, 781, 1216, 1271
HpyF3I CTNAG 5 cut(s) 213, 399, 536, 956, 1228
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 4 cut(s) 30, 50, 353, 1078
HspAI GCGC 1 cut(s) 43
Kzo9I GATC 3 cut(s) 1136, 1310, 1344
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 1 cut(s) 1237
Lsp1109I GCAGC 2 cut(s) 1144, 1252
LweI GCATC 3 cut(s) 297, 730, 1074
MaeI CTAG 3 cut(s) 600, 663, 681
MaeIII GTNAC 2 cut(s) 257, 723
MalI GATC 3 cut(s) 1138, 1312, 1346
MboI GATC 3 cut(s) 1136, 1310, 1344
MboII GAAGA 6 cut(s) 145, 542, 740, 743, 746, 817
MflI RGATCY 1 cut(s) 1310
MhlI GDGCHC 2 cut(s) 17, 1221
MlsI TGGCCA 2 cut(s) 130, 1087
MluCI AATT 6 cut(s) 93, 164, 375, 691, 1161, 1421
MluNI TGGCCA 2 cut(s) 130, 1087
MlyI GAGTC 1 cut(s) 1331
Mox20I TGGCCA 2 cut(s) 130, 1087
MroXI GAANNNNTTC 2 cut(s) 152, 467
MscI TGGCCA 2 cut(s) 130, 1087
MseI TTAA 6 cut(s) 92, 228, 863, 890, 1352, 1406
MslI CAYNNNNRTG 1 cut(s) 1073
Msp20I TGGCCA 2 cut(s) 130, 1087
MspA1I CMGCKG 1 cut(s) 1243
MspI CCGG 3 cut(s) 221, 276, 830
MspR9I CCNGG 5 cut(s) 221, 222, 277, 1017, 1203
Mva1269I GAATGC 1 cut(s) 817
MvaI CCWGG 2 cut(s) 1017, 1203
MwoI GCNNNNNNNGC 5 cut(s) 49, 346, 781, 1216, 1271
NciI CCSGG 3 cut(s) 221, 222, 277
NcoI CCATGG 1 cut(s) 46
NdeII GATC 3 cut(s) 1136, 1310, 1344
NlaIII CATG 4 cut(s) 30, 50, 353, 1078
NlaIV GGNNCC 1 cut(s) 444
NspV TTCGAA 1 cut(s) 21
PaqCI CACCTGC 1 cut(s) 789
PceI AGGCCT 1 cut(s) 990
PciSI GCTCTTC 1 cut(s) 158
PctI GAATGC 1 cut(s) 817
PdmI GAANNNNTTC 2 cut(s) 152, 467
PfeI GAWTC 5 cut(s) 244, 386, 532, 1246, 1399
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 3 cut(s) 373, 1134, 1242
PleI GAGTC 1 cut(s) 1331
PpsI GAGTC 1 cut(s) 1331
Psp124BI GAGCTC 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 1015, 1201
PspGI CCWGG 2 cut(s) 1015, 1201
PspN4I GGNNCC 1 cut(s) 444
PspPI GGNCC 4 cut(s) 64, 327, 443, 719
PsuI RGATCY 1 cut(s) 1310
PvuII CAGCTG 1 cut(s) 1243
RsaI GTAC 2 cut(s) 39, 961
RsaNI GTAC 2 cut(s) 38, 960
RseI CAYNNNNRTG 1 cut(s) 1073
Rsr2I CGGWCCG 1 cut(s) 64
RsrII CGGWCCG 1 cut(s) 64
SacI GAGCTC 1 cut(s) 1221
SalI GTCGAC 1 cut(s) 1383
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 6 cut(s) 92, 228, 863, 890, 1352, 1406
SatI GCNGC 3 cut(s) 372, 1133, 1241
Sau3AI GATC 3 cut(s) 1136, 1310, 1344
Sau96I GGNCC 4 cut(s) 64, 327, 443, 719
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 5 cut(s) 221, 222, 277, 1017, 1203
SduI GDGCHC 2 cut(s) 17, 1221
SfaNI GCATC 3 cut(s) 297, 730, 1074
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 1 cut(s) 64
SmaI CCCGGG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 1 cut(s) 1002
SmoI CTYRAG 1 cut(s) 1002
Sse9I AATT 6 cut(s) 93, 164, 375, 691, 1161, 1421
SseBI AGGCCT 1 cut(s) 990
SsiI CCGC 5 cut(s) 62, 67, 113, 281, 371
SspMI CTAG 3 cut(s) 600, 663, 681
SstI GAGCTC 1 cut(s) 1221
StuI AGGCCT 1 cut(s) 990
StyD4I CCNGG 5 cut(s) 219, 220, 275, 1015, 1201
StyI CCWWGG 2 cut(s) 46, 1122
TaaI ACNGT 3 cut(s) 459, 574, 915
TaqI TCGA 7 cut(s) 9, 21, 146, 156, 162, 1384, 1397
TasI AATT 6 cut(s) 93, 164, 375, 691, 1161, 1421
TatI WGTACW 1 cut(s) 959
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 5 cut(s) 244, 386, 532, 1246, 1399
Tru1I TTAA 6 cut(s) 92, 228, 863, 890, 1352, 1406
Tru9I TTAA 6 cut(s) 92, 228, 863, 890, 1352, 1406
TscAI CASTG 4 cut(s) 570, 765, 1110, 1236
TseI GCWGC 2 cut(s) 1132, 1240
TspDTI ATGAA 2 cut(s) 113, 684
TspMI CCCGGG 1 cut(s) 220
TspRI CASTG 4 cut(s) 570, 765, 1110, 1236
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 2 cut(s) 74, 632
XapI RAATTY 2 cut(s) 164, 1421
XbaI TCTAGA 1 cut(s) 680
XmaI CCCGGG 1 cut(s) 220
XmiI GTMKAC 2 cut(s) 559, 1384
XmnI GAANNNNTTC 2 cut(s) 152, 467
XspI CTAG 3 cut(s) 600, 663, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.