Rh5AG209400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004081
Physical Location & Seq
Reverse (-)
24715019 .. 24720143
5125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG209400.1

Sequence Viewer

Length: 1185 bp
ATGTCGGTGGAGAGGTCCTTCGAGGCTTGGGAGGAGGTGCAGCGTCACGGCCAGGACCTAGCGGATCGTCTGGCGCAGGGTTTCACCGGTCTCATTCAGACCCACATCAATACGCCGTCGTTTCCGTGGCCGAGCTCTCACAATTCGAAGCTGTTCGACCTCGAGTTCCCGAGCCAGAGCTTCGGGAAGAGGGACTTGGTCGGGTTGGTGGCGGAGAACCCCGGAATCAATGGGGTTTCGGCGATTCTGGACATAGGGAACAGGATTGGGCAGGCCGGGGCCGATTTCGGGGCCTGCTTGGATGGGATGGTGCACCAGTTCTTTCGGAGCTTGCCGGTGCCGTTCCGGCACGAGGAGAATTTGGGGCCGTTGATTCGGGTCGAGTCGGATAAGGTTAATCTGAGAGCTGATGTGGGTGTGAGTAGTAAGGAGGATTTGGGGCCGGTAGTGAGGGATTCTAGGGGTGTGGAGAGTGATAGTGGCTCTGATGGGTTGGTGGATGAAGACCTTGCTGGCTTGAATCTAAGGTCTGCTGGGCTTCTGGGTAGACCACAGGGCATCCTAAATACTACAACAACTTACGATAGTAGAACACGTGAGCTAGAAAGCTCTTTGGTTGCAAGGGGAGATTTGTGGAGAGTAGAGGCTTCTCAAGGCAGTTCAACATCTAGAAATGGCAATTCATCACTCTTTCTTGTTCAGCTTGGACCAGTCCTCTTTGTTCGTGATGCGACTCTTCTTTTGCCAGTGCATTTGTCAAAGCAACACTTGCTTTGGTATGGCTATGACAGGAAGAATGGGCTGCATTCTCTCTGTCCGGCAGTGTGGTCAAAACACAGAAGGTGGCTCTTAATGTCAATGCTCTGTCTGAACCCCGTAGCCTGTTCATTTGTTGATTTACAGTTTCCAAATGGGCAATTCACATATGTATCTGGTGAAGGGCTTAGTACAAGTGCTTTCTTACCTCTCTGTGGAGGTCTTCTACAAGCTCAAGGTCAATATCCTGGAGAAATGAGATTCAGTTACTCTCGCAAGAATAAATGGGGAACACGTATCACACCAATGGTACAATGGCCAGACAAGTCTTTTACACTGGGTCTTTCTCAAGCCTTGGCTTGGCAGACATCTGGTCTCATGGTGCGGCCTACACTTCAGTGCAGGTTAGGTTTATGCTGTCTCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

43.44

Weight (kDa)

6.76

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1149
AccB1I GGYRCC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 547
AciI CCGC 3 cut(s) 62, 212, 1141
AclWI GGATC 1 cut(s) 72
AcoI YGGCCR 3 cut(s) 49, 128, 1073
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 1 cut(s) 1136
AcvI CACGTG 1 cut(s) 596
AfaI GTAC 2 cut(s) 949, 1068
AfiI CCNNNNNNNGG 4 cut(s) 288, 352, 971, 1116
AflIII ACRYGT 2 cut(s) 593, 1049
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 2 cut(s) 520, 663
AhdI GACNNNNNGTC 1 cut(s) 1128
AjnI CCWGG 2 cut(s) 51, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 179, 211
AleI CACNNNNGTG 1 cut(s) 1153
AluBI AGCT 9 cut(s) 135, 151, 180, 330, 407, 601, 609, 703, 989
AluI AGCT 9 cut(s) 135, 151, 180, 330, 407, 601, 609, 703, 989
Alw21I GWGCWC 2 cut(s) 137, 315
Alw26I GTCTC 3 cut(s) 95, 1136, 1181
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 1 cut(s) 72
Ama87I CYCGRG 2 cut(s) 161, 169
AoxI GGCC 9 cut(s) 49, 128, 273, 279, 291, 365, 440, 1073, 1142
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 40, 802
ApoI RAATTY 1 cut(s) 358
ArsI GACNNNNNNTTYG 2 cut(s) 724, 756
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 1 cut(s) 152
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 7 cut(s) 15, 55, 279, 291, 365, 440, 707
AsuC2I CCSGG 2 cut(s) 222, 277
AsuHPI GGTGA 2 cut(s) 76, 947
AsuII TTCGAA 1 cut(s) 146
AvaI CYCGRG 2 cut(s) 161, 169
AvaII GGWCC 3 cut(s) 15, 55, 707
BaeGI GKGCMC 1 cut(s) 315
BaeI ACNNNNGTAYC 2 cut(s) 912, 945
BalI TGGCCA 1 cut(s) 1075
BanI GGYRCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 137
BauI CACGAG 1 cut(s) 350
BbrPI CACGTG 1 cut(s) 596
BbsI GAAGAC 2 cut(s) 510, 971
Bbv12I GWGCWC 2 cut(s) 137, 315
BbvI GCAGC 2 cut(s) 52, 789
BccI CCATC 3 cut(s) 296, 301, 482
BceAI ACGGC 4 cut(s) 64, 100, 325, 352
BciT130I CCWGG 2 cut(s) 53, 1005
BcnI CCSGG 2 cut(s) 222, 277
BcoDI GTCTC 3 cut(s) 95, 1136, 1181
BfaI CTAG 4 cut(s) 59, 459, 602, 669
BfuAI ACCTGC 1 cut(s) 1149
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 41, 803, 1142
BlsI GCNGC 3 cut(s) 42, 804, 1143
Bme1390I CCNGG 4 cut(s) 53, 222, 277, 1005
Bme18I GGWCC 3 cut(s) 15, 55, 707
BmeRI GACNNNNNGTC 1 cut(s) 1128
BmeT110I CYCGRG 2 cut(s) 161, 169
BmgT120I GGNCC 7 cut(s) 15, 55, 279, 291, 365, 440, 707
BmiI GGNNCC 5 cut(s) 280, 292, 339, 366, 441
BmrFI CCNGG 4 cut(s) 53, 222, 277, 1005
BmrI ACTGGG 1 cut(s) 1103
BmsI GCATC 2 cut(s) 567, 718
BmuI ACTGGG 1 cut(s) 1103
BpiI GAAGAC 2 cut(s) 510, 971
BpmI CTGGAG 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 3 cut(s) 636, 975, 1089
BpuMI CCSGG 2 cut(s) 222, 277
BsaAI YACGTR 2 cut(s) 596, 1052
BsaI GGTCTC 2 cut(s) 95, 1136
BsaJI CCNNGG 4 cut(s) 125, 220, 276, 1110
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 352, 971, 1116
Bse118I RCCGGY 3 cut(s) 86, 334, 442
Bse1I ACTGG 4 cut(s) 316, 710, 746, 1098
BseBI CCWGG 2 cut(s) 53, 1005
BseDI CCNNGG 4 cut(s) 125, 220, 276, 1110
BseGI GGATG 4 cut(s) 307, 312, 505, 558
BseLI CCNNNNNNNGG 4 cut(s) 288, 352, 971, 1116
BseMII CTCAG 1 cut(s) 392
BseNI ACTGG 4 cut(s) 316, 710, 746, 1098
BseRI GAGGAG 2 cut(s) 47, 368
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 2 cut(s) 52, 789
BseYI CCCAGC 1 cut(s) 533
BsgI GTGCAG 2 cut(s) 59, 1177
BshFI GGCC 9 cut(s) 51, 130, 275, 281, 293, 367, 442, 1075, 1144
BshNI GGYRCC 1 cut(s) 337
BshTI ACCGGT 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 137, 315
BsiHKCI CYCGRG 2 cut(s) 161, 169
BsiSI CCGG 7 cut(s) 87, 222, 276, 335, 346, 443, 818
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 4 cut(s) 288, 352, 971, 1116
BsmAI GTCTC 3 cut(s) 95, 1136, 1181
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 9 cut(s) 51, 130, 275, 281, 293, 367, 442, 1075, 1144
Bso31I GGTCTC 2 cut(s) 95, 1136
BsoBI CYCGRG 2 cut(s) 161, 169
Bsp119I TTCGAA 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 137, 315
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 3 cut(s) 62, 212, 1141
BspANI GGCC 9 cut(s) 51, 130, 275, 281, 293, 367, 442, 1075, 1144
BspCNI CTCAG 1 cut(s) 393
BspLI GGNNCC 5 cut(s) 280, 292, 339, 366, 441
BspMI ACCTGC 1 cut(s) 1149
BspPI GGATC 1 cut(s) 72
BspT104I TTCGAA 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 337
BspTNI GGTCTC 2 cut(s) 95, 1136
BsrFI RCCGGY 3 cut(s) 86, 334, 442
BsrI ACTGG 4 cut(s) 316, 710, 746, 1098
BssAI RCCGGY 3 cut(s) 86, 334, 442
BssECI CCNNGG 4 cut(s) 125, 220, 276, 1110
BssMI GATC 1 cut(s) 64
BssSI CACGAG 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 1110
Bst2BI CACGAG 1 cut(s) 350
Bst2UI CCWGG 2 cut(s) 53, 1005
Bst4CI ACNGT 1 cut(s) 903
Bst6I CTCTTC 2 cut(s) 182, 741
BstAPI GCANNNNNTGC 1 cut(s) 769
BstBAI YACGTR 2 cut(s) 596, 1052
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 4 cut(s) 273, 295, 332, 514
BstDEI CTNAG 3 cut(s) 401, 524, 944
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 4 cut(s) 307, 312, 505, 558
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 3 cut(s) 95, 1136, 1181
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 346, 769
BstNI CCWGG 2 cut(s) 53, 1005
BstSCI CCNGG 4 cut(s) 51, 220, 275, 1003
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 52, 789
BstV2I GAAGAC 2 cut(s) 510, 971
BsuRI GGCC 9 cut(s) 51, 130, 275, 281, 293, 367, 442, 1075, 1144
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 4 cut(s) 307, 312, 505, 558
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 4 cut(s) 753, 828, 1091, 1160
BveI ACCTGC 1 cut(s) 1149
Cac8I GCNNGC 4 cut(s) 273, 295, 332, 514
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 3 cut(s) 86, 334, 442
Cfr13I GGNCC 7 cut(s) 15, 55, 279, 291, 365, 440, 707
CseI GACGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 948, 1067
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 1 cut(s) 1135
CviQI GTAC 2 cut(s) 948, 1067
DdeI CTNAG 3 cut(s) 401, 524, 944
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
DriI GACNNNNNGTC 1 cut(s) 1128
EaeI YGGCCR 3 cut(s) 49, 128, 1073
Eam1104I CTCTTC 2 cut(s) 182, 741
Eam1105I GACNNNNNGTC 1 cut(s) 1128
EarI CTCTTC 2 cut(s) 182, 741
EciI GGCGGA 1 cut(s) 227
Ecl136II GAGCTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 1110
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 2 cut(s) 95, 1136
Eco47I GGWCC 3 cut(s) 15, 55, 707
Eco53kI GAGCTC 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 1136
Eco72I CACGTG 1 cut(s) 596
Eco88I CYCGRG 2 cut(s) 161, 169
EcoICRI GAGCTC 1 cut(s) 135
EcoO109I RGGNCCY 3 cut(s) 15, 55, 291
EcoRII CCWGG 2 cut(s) 51, 1003
EcoT14I CCWWGG 1 cut(s) 1110
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 1110
FaeI CATG 1 cut(s) 1138
FaiI YATR 7 cut(s) 254, 780, 786, 925, 927, 1136, 1171
FalI AAGNNNNNCTT 4 cut(s) 179, 211, 752, 784
FaqI GGGAC 1 cut(s) 206
FatI CATG 1 cut(s) 1134
FauNDI CATATG 1 cut(s) 925
FblI GTMKAC 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 41, 803, 1142
FokI GGATG 4 cut(s) 314, 319, 512, 545
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 3 cut(s) 41, 803, 1142
FspBI CTAG 4 cut(s) 59, 459, 602, 669
GlaI GCGC 1 cut(s) 75
GluI GCNGC 3 cut(s) 41, 803, 1142
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 9 cut(s) 51, 130, 275, 281, 293, 367, 442, 1075, 1144
HapII CCGG 7 cut(s) 87, 222, 276, 335, 346, 443, 818
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 76
Hin1II CATG 1 cut(s) 1138
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 8 cut(s) 225, 244, 373, 383, 455, 520, 733, 1017
HpaII CCGG 7 cut(s) 87, 222, 276, 335, 346, 443, 818
HphI GGTGA 2 cut(s) 76, 947
Hpy166II GTNNAC 2 cut(s) 313, 548
Hpy188I TCNGA 6 cut(s) 99, 327, 388, 402, 487, 870
Hpy188III TCNNGA 5 cut(s) 169, 184, 248, 669, 725
Hpy8I GTNNAC 2 cut(s) 313, 548
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 28, 834, 932
HpyCH4III ACNGT 1 cut(s) 903
HpyCH4IV ACGT 2 cut(s) 595, 1051
HpyCH4V TGCA 6 cut(s) 40, 313, 620, 751, 805, 1158
HpyF10VI GCNNNNNNNGC 2 cut(s) 346, 769
HpyF3I CTNAG 3 cut(s) 401, 524, 944
HpySE526I ACGT 2 cut(s) 595, 1051
Hsp92II CATG 1 cut(s) 1138
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 52, 789
LweI GCATC 2 cut(s) 567, 718
MaeI CTAG 4 cut(s) 59, 459, 602, 669
MaeII ACGT 2 cut(s) 595, 1051
MaeIII GTNAC 2 cut(s) 44, 1022
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 5 cut(s) 199, 515, 728, 805, 971
MhlI GDGCHC 2 cut(s) 137, 315
MlsI TGGCCA 1 cut(s) 1075
MluCI AATT 4 cut(s) 142, 358, 679, 917
MluNI TGGCCA 1 cut(s) 1075
MlyI GAGTC 2 cut(s) 392, 727
MmeI TCCRAC 1 cut(s) 366
Mox20I TGGCCA 1 cut(s) 1075
MroXI GAANNNNTTC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 1075
MseI TTAA 2 cut(s) 396, 851
MslI CAYNNNNRTG 2 cut(s) 1061, 1153
Msp20I TGGCCA 1 cut(s) 1075
MspI CCGG 7 cut(s) 87, 222, 276, 335, 346, 443, 818
MspR9I CCNGG 4 cut(s) 53, 222, 277, 1005
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 2 cut(s) 53, 1005
MwoI GCNNNNNNNGC 2 cut(s) 346, 769
NciI CCSGG 2 cut(s) 222, 277
NdeI CATATG 1 cut(s) 925
NdeII GATC 1 cut(s) 64
NlaIII CATG 1 cut(s) 1138
NlaIV GGNNCC 5 cut(s) 280, 292, 339, 366, 441
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 44
NspV TTCGAA 1 cut(s) 146
OliI CACNNNNGTG 1 cut(s) 1153
PaeR7I CTCGAG 1 cut(s) 161
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 1 cut(s) 152
PfeI GAWTC 6 cut(s) 225, 244, 373, 455, 520, 1017
PflFI GACNNNGTC 1 cut(s) 197
PfoI TCCNGGA 1 cut(s) 1003
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 3 cut(s) 42, 804, 1143
PleI GAGTC 2 cut(s) 391, 727
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 2 cut(s) 391, 727
Ppu21I YACGTR 2 cut(s) 596, 1052
PpuMI RGGWCCY 2 cut(s) 15, 55
Psp124BI GAGCTC 1 cut(s) 137
Psp5II RGGWCCY 2 cut(s) 15, 55
Psp6I CCWGG 2 cut(s) 51, 1003
PspCI CACGTG 1 cut(s) 596
PspFI CCCAGC 1 cut(s) 533
PspGI CCWGG 2 cut(s) 51, 1003
PspN4I GGNNCC 5 cut(s) 280, 292, 339, 366, 441
PspPI GGNCC 7 cut(s) 15, 55, 279, 291, 365, 440, 707
PspPPI RGGWCCY 2 cut(s) 15, 55
PspXI VCTCGAGB 1 cut(s) 161
PsyI GACNNNGTC 1 cut(s) 197
RsaI GTAC 2 cut(s) 949, 1068
RsaNI GTAC 2 cut(s) 948, 1067
RseI CAYNNNNRTG 2 cut(s) 1061, 1153
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 2 cut(s) 396, 851
SatI GCNGC 3 cut(s) 41, 803, 1142
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 7 cut(s) 15, 55, 279, 291, 365, 440, 707
SchI GAGTC 2 cut(s) 392, 727
ScrFI CCNGG 4 cut(s) 53, 222, 277, 1005
SduI GDGCHC 2 cut(s) 137, 315
SfaNI GCATC 2 cut(s) 567, 718
Sfr274I CTCGAG 1 cut(s) 161
SfuI TTCGAA 1 cut(s) 146
SinI GGWCC 3 cut(s) 15, 55, 707
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 2 cut(s) 1061, 1153
SmlI CTYRAG 4 cut(s) 161, 651, 990, 1104
SmoI CTYRAG 4 cut(s) 161, 651, 990, 1104
Sse9I AATT 4 cut(s) 142, 358, 679, 917
SsiI CCGC 3 cut(s) 62, 212, 1141
SspMI CTAG 4 cut(s) 59, 459, 602, 669
SstI GAGCTC 1 cut(s) 137
StyD4I CCNGG 4 cut(s) 51, 220, 275, 1003
StyI CCWWGG 1 cut(s) 1110
TaaI ACNGT 1 cut(s) 903
TaiI ACGT 2 cut(s) 598, 1054
TaqI TCGA 5 cut(s) 21, 146, 156, 162, 381
TasI AATT 4 cut(s) 142, 358, 679, 917
TatI WGTACW 1 cut(s) 947
TauI GCSGC 1 cut(s) 1144
TfiI GAWTC 6 cut(s) 225, 244, 373, 455, 520, 1017
Tru1I TTAA 2 cut(s) 396, 851
Tru9I TTAA 2 cut(s) 396, 851
TscAI CASTG 4 cut(s) 753, 828, 1098, 1160
TseFI GTSAC 1 cut(s) 44
TseI GCWGC 2 cut(s) 40, 802
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 3 cut(s) 516, 672, 876
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 4 cut(s) 753, 828, 1098, 1160
Tth111I GACNNNGTC 1 cut(s) 197
VneI GTGCAC 1 cut(s) 311
VpaK11BI GGWCC 3 cut(s) 15, 55, 707
XapI RAATTY 1 cut(s) 358
XbaI TCTAGA 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 1068
XhoI CTCGAG 1 cut(s) 161
XmiI GTMKAC 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 152
XspI CTAG 4 cut(s) 59, 459, 602, 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.