pycom11g23510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0004081
Physical Location & Seq
Forward (+)
26483179 .. 26488117
4939 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g23510.1

Sequence Viewer

Length: 1398 bp
ATGTCGGTCGAGAGCCCCTTCGAAGCATGGGAGGAGGTACAGCGCCATGGGCAGGACTTGGCGGACCGCCTCACGCAGGGCTTCACGGGTTTAATTCAGTCCCACATCACTCCGCCGTCGTTTCATTGGCCAAACCCTAACACATCGAAGCTCTTCGACCTCGAATTCCCGAACCAGAGTTTTGGCAAGAGGGATTTTGCTGGGTTGGTGGCTGAGAACCCGGGCATTAACGGGGTTTCTGCGATTCTTGATATCGGTAACAGGATTGGGCAAGCGGGGGCGGATTTTGGGGCGTGCTTGAATGGGATGGTTCAGCAGTTCTTTAGGGCCTTACCCGTGCCGTTTAGGCATGATGAGAATGTTGATTTGGCGGCAATTCGGATTGATTCGCGTAAGGCTAAGCACAGAGCTGATATGATTTTGAGTGGCAAGGAGGATTTGGGGCCATTGAATGAACGGTTGAAGGATTTCAGGGTTGTGGAGAGTGATGGTGGCTCTGATGGTGTGGTGGATGAGGAGCTTGGTGGCTTGAATCTAAGGTCTGCCGGTCTTCTGGGTAGACCACTGGGCACTGTAAATAGCACAACAAGTTATGATACTAGAACCCGTAAGCTGGAAAGTTCTTTCCATGCAAGGGGAGACTTATGGAGAGTAGAGGCTTCTAGTGGCAGTTCCACATCTAGAAATGACAATTCATCACTTTTCCTTGTCCAGCTTGGGCCATTACTCTTTGTTCGTGATGCAACTCTTCTTCTTCCTGTGCATTTGTCAAAGCAACACCTGCTTTGGTATGGCTATGATAGAAAGTGTCGGCACTATTATTGGGTCTACGGCATTGGTTCTCATGATCACTCTCTCTGTGCCCAGTCATTTGTTGATTTACAGTTTCCAAACGGGCAGTTTACATATATATCTGGTGAAGGACTTAGTACAAGTGCTTTCTTACCTCTTTGTGGAGGCCTTCTACAAGCTCAAGGTCAATATCCTGGAGAAATGATATTCAGTTTCTCTCGCAAGAATAAGTGGGGAACACGCATCACACCCATGATACAATGGCCAGACAAGTCTTTTACACTGGGTCTTTCGCAAGCCTTGGCTTGGCAGCGATCGGGTCTTATGGTGAAGCCTACAATTCAGTTCAGTTTATGTCCCACTATTGGTGGAAGCAATCCTGGTTTGCGGGCAGAGCTCATTCACTCAGTGAAGGAGCAGCTGAATCTTATATGTGGTGGTTCATTTACCGCACATCCTTCTGCTTTTGCTTCAATATCGGTTGGCAGATCCAAGTGGAATGGAAATGTTGGGGACTCGGGGATCGTTTTAAGAGTGGATGCTCCTCTTTCCAATGTTGGTCGACCATACTTGTCGATTCAGTTAAATAATGTTCTTGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

466

Amino Acids

51.11

Weight (kDa)

6.9

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 789
AccI GTMKAC 3 cut(s) 559, 828, 1354
AccII CGCG 1 cut(s) 391
AciI CCGC 8 cut(s) 62, 67, 113, 275, 281, 371, 1180, 1242
AclWI GGATC 2 cut(s) 1275, 1322
AcoI YGGCCR 2 cut(s) 128, 1055
AcsI RAATTY 2 cut(s) 164, 1391
AdeI CACNNNGTG 1 cut(s) 1201
AfaI GTAC 2 cut(s) 39, 931
AfiI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 953, 1098, 1157
AgsI TTSAA 6 cut(s) 301, 451, 463, 532, 1266, 1391
AjnI CCWGG 2 cut(s) 985, 1171
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 8 cut(s) 151, 410, 520, 613, 715, 971, 1189, 1213
AluI AGCT 8 cut(s) 151, 410, 520, 613, 715, 971, 1189, 1213
Alw21I GWGCWC 1 cut(s) 1191
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 2 cut(s) 1275, 1322
Ama87I CYCGRG 2 cut(s) 220, 1309
AoxI GGCC 6 cut(s) 128, 327, 443, 719, 958, 1055
ApeKI GCWGC 2 cut(s) 1102, 1210
ApoI RAATTY 2 cut(s) 164, 1391
Asp700I GAANNNNTTC 2 cut(s) 152, 467
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 4 cut(s) 64, 327, 443, 719
AsuC2I CCSGG 2 cut(s) 221, 222
AsuHPI GGTGA 2 cut(s) 929, 1132
AsuII TTCGAA 1 cut(s) 21
AvaI CYCGRG 2 cut(s) 220, 1309
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 2 cut(s) 572, 865
BalI TGGCCA 2 cut(s) 130, 1057
BanII GRGCYC 2 cut(s) 17, 1191
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 1191
BbvI GCAGC 2 cut(s) 1114, 1222
BccI CCATC 3 cut(s) 301, 482, 494
BceAI ACGGC 3 cut(s) 100, 325, 847
BciT130I CCWGG 2 cut(s) 987, 1173
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 2 cut(s) 221, 222
BcoDI GTCTC 1 cut(s) 633
BfaI CTAG 3 cut(s) 600, 663, 681
BfoI RGCGCY 1 cut(s) 46
BfuAI ACCTGC 1 cut(s) 789
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 372, 1103, 1211
BlpI GCTNAGC 1 cut(s) 399
BlsI GCNGC 3 cut(s) 373, 1104, 1212
Bme1390I CCNGG 4 cut(s) 221, 222, 987, 1173
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 220, 1309
BmgT120I GGNCC 4 cut(s) 64, 327, 443, 719
BmiI GGNNCC 1 cut(s) 444
BmrFI CCNGG 4 cut(s) 221, 222, 987, 1173
BmrI ACTGGG 3 cut(s) 575, 859, 1085
BmsI GCATC 3 cut(s) 730, 1044, 1321
BmuI ACTGGG 3 cut(s) 575, 859, 1085
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 1008
Bpu1102I GCTNAGC 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 957
BpuMI CCSGG 2 cut(s) 221, 222
BsaJI CCNNGG 3 cut(s) 46, 220, 1092
Bsc4I CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 953, 1098, 1157
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 3 cut(s) 570, 865, 1080
BseBI CCWGG 2 cut(s) 987, 1173
BseDI CCNNGG 3 cut(s) 46, 220, 1092
BseGI GGATG 4 cut(s) 312, 517, 1246, 1336
BseLI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 953, 1098, 1157
BseMII CTCAG 2 cut(s) 204, 1212
BseNI ACTGG 3 cut(s) 570, 865, 1080
BseRI GAGGAG 3 cut(s) 47, 530, 1326
BseSI GKGCMC 2 cut(s) 572, 865
BseXI GCAGC 2 cut(s) 1114, 1222
BseYI CCCAGC 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 391
Bsh1285I CGRYCG 2 cut(s) 9, 1109
BshFI GGCC 6 cut(s) 130, 329, 445, 721, 960, 1057
BsiEI CGRYCG 2 cut(s) 9, 1109
BsiHKAI GWGCWC 1 cut(s) 1191
BsiHKCI CYCGRG 2 cut(s) 220, 1309
BsiSI CCGG 2 cut(s) 221, 546
BslFI GGGAC 3 cut(s) 85, 1134, 1319
BslI CCNNNNNNNGG 7 cut(s) 52, 76, 613, 634, 953, 1098, 1157
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 3 cut(s) 85, 1134, 1319
BsnI GGCC 6 cut(s) 130, 329, 445, 721, 960, 1057
BsoBI CYCGRG 2 cut(s) 220, 1309
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 4 cut(s) 17, 572, 865, 1191
Bsp143I GATC 4 cut(s) 847, 1106, 1280, 1314
Bsp1720I GCTNAGC 1 cut(s) 399
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 8 cut(s) 62, 67, 113, 275, 281, 371, 1180, 1242
BspANI GGCC 6 cut(s) 130, 329, 445, 721, 960, 1057
BspCNI CTCAG 2 cut(s) 205, 1211
BspFNI CGCG 1 cut(s) 391
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 1 cut(s) 444
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 2 cut(s) 1275, 1322
BspQI GCTCTTC 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 3 cut(s) 570, 865, 1080
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 3 cut(s) 46, 220, 1092
BssMI GATC 4 cut(s) 847, 1106, 1280, 1314
BssT1I CCWWGG 2 cut(s) 46, 1092
Bst2UI CCWGG 2 cut(s) 987, 1173
Bst4CI ACNGT 3 cut(s) 459, 574, 885
Bst6I CTCTTC 2 cut(s) 158, 753
BstAPI GCANNNNNTGC 1 cut(s) 781
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 4 cut(s) 273, 295, 1089, 1182
BstDEI CTNAG 5 cut(s) 213, 399, 536, 926, 1198
BstDSI CCRYGG 1 cut(s) 46
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 4 cut(s) 312, 517, 1246, 1336
BstFNI CGCG 1 cut(s) 391
BstH2I RGCGCY 1 cut(s) 46
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 4 cut(s) 850, 1109, 1283, 1317
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 4 cut(s) 847, 1106, 1280, 1314
BstMCI CGRYCG 2 cut(s) 9, 1109
BstMWI GCNNNNNNNGC 4 cut(s) 49, 346, 781, 1186
BstNI CCWGG 2 cut(s) 987, 1173
BstSCI CCNGG 4 cut(s) 219, 220, 985, 1171
BstSLI GKGCMC 2 cut(s) 572, 865
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 2 cut(s) 1114, 1222
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 1280
BstXI CCANNNNNNTGG 1 cut(s) 182
BstYI RGATCY 1 cut(s) 1280
BsuRI GGCC 6 cut(s) 130, 329, 445, 721, 960, 1057
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 4 cut(s) 312, 517, 1246, 1336
BtsIMutI CAGTG 4 cut(s) 563, 570, 1073, 1206
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 4 cut(s) 273, 295, 1089, 1182
CciI TCATGA 1 cut(s) 844
CfoI GCGC 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 4 cut(s) 64, 327, 443, 719
Cfr9I CCCGGG 1 cut(s) 220
CpoI CGGWCCG 1 cut(s) 64
Csp6I GTAC 2 cut(s) 38, 930
CspI CGGWCCG 1 cut(s) 64
CviAII CATG 6 cut(s) 27, 47, 350, 629, 845, 1045
CviQI GTAC 2 cut(s) 38, 930
DdeI CTNAG 5 cut(s) 213, 399, 536, 926, 1198
DpnI GATC 4 cut(s) 849, 1108, 1282, 1316
DpnII GATC 4 cut(s) 847, 1106, 1280, 1314
DraIII CACNNNGTG 1 cut(s) 1201
EaeI YGGCCR 2 cut(s) 128, 1055
Eam1104I CTCTTC 2 cut(s) 158, 753
EarI CTCTTC 2 cut(s) 158, 753
EciI GGCGGA 3 cut(s) 77, 102, 296
Ecl136II GAGCTC 1 cut(s) 1189
Eco130I CCWWGG 2 cut(s) 46, 1092
Eco147I AGGCCT 1 cut(s) 960
Eco24I GRGCYC 2 cut(s) 17, 1191
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 1189
Eco88I CYCGRG 2 cut(s) 220, 1309
EcoICRI GAGCTC 1 cut(s) 1189
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 985, 1171
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 46, 1092
EcoT38I GRGCYC 2 cut(s) 17, 1191
ErhI CCWWGG 2 cut(s) 46, 1092
FaeI CATG 6 cut(s) 30, 50, 353, 632, 848, 1048
FaqI GGGAC 3 cut(s) 85, 1134, 1319
FatI CATG 6 cut(s) 26, 46, 349, 628, 844, 1044
FauI CCCGC 2 cut(s) 268, 1173
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 3 cut(s) 559, 828, 1354
Fnu4HI GCNGC 3 cut(s) 372, 1103, 1211
FokI GGATG 4 cut(s) 319, 524, 1233, 1343
FriOI GRGCYC 2 cut(s) 17, 1191
Fsp4HI GCNGC 3 cut(s) 372, 1103, 1211
FspBI CTAG 3 cut(s) 600, 663, 681
GlaI GCGC 1 cut(s) 44
GluI GCNGC 3 cut(s) 372, 1103, 1211
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 1008
HaeII RGCGCY 1 cut(s) 46
HaeIII GGCC 6 cut(s) 130, 329, 445, 721, 960, 1057
HapII CCGG 2 cut(s) 221, 546
HhaI GCGC 1 cut(s) 45
Hin1II CATG 6 cut(s) 30, 50, 353, 632, 848, 1048
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HincII GTYRAC 1 cut(s) 1355
HindII GTYRAC 1 cut(s) 1355
HinfI GANTC 6 cut(s) 244, 386, 532, 1216, 1307, 1369
HpaII CCGG 2 cut(s) 221, 546
HphI GGTGA 2 cut(s) 929, 1132
Hpy166II GTNNAC 4 cut(s) 560, 829, 903, 1355
Hpy188I TCNGA 2 cut(s) 381, 499
Hpy188III TCNNGA 7 cut(s) 10, 169, 248, 681, 737, 845, 1388
Hpy8I GTNNAC 4 cut(s) 560, 829, 903, 1355
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 6 cut(s) 28, 457, 914, 971, 1198, 1260
HpyCH4III ACNGT 3 cut(s) 459, 574, 885
HpyCH4V TGCA 3 cut(s) 632, 743, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 346, 781, 1186
HpyF3I CTNAG 5 cut(s) 213, 399, 536, 926, 1198
Hsp92II CATG 6 cut(s) 30, 50, 353, 632, 848, 1048
HspAI GCGC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 4 cut(s) 847, 1106, 1280, 1314
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 3 cut(s) 517, 1207, 1339
Lsp1109I GCAGC 2 cut(s) 1114, 1222
LweI GCATC 3 cut(s) 730, 1044, 1321
MaeI CTAG 3 cut(s) 600, 663, 681
MaeIII GTNAC 1 cut(s) 257
MalI GATC 4 cut(s) 849, 1108, 1282, 1316
MboI GATC 4 cut(s) 847, 1106, 1280, 1314
MboII GAAGA 5 cut(s) 145, 542, 740, 743, 746
MflI RGATCY 1 cut(s) 1280
MhlI GDGCHC 4 cut(s) 17, 572, 865, 1191
MlsI TGGCCA 2 cut(s) 130, 1057
MluCI AATT 6 cut(s) 93, 164, 375, 691, 1131, 1391
MluNI TGGCCA 2 cut(s) 130, 1057
MlyI GAGTC 1 cut(s) 1301
Mox20I TGGCCA 2 cut(s) 130, 1057
MroXI GAANNNNTTC 2 cut(s) 152, 467
MscI TGGCCA 2 cut(s) 130, 1057
MseI TTAA 4 cut(s) 92, 228, 1322, 1376
MslI CAYNNNNRTG 1 cut(s) 1043
Msp20I TGGCCA 2 cut(s) 130, 1057
MspA1I CMGCKG 1 cut(s) 1213
MspI CCGG 2 cut(s) 221, 546
MspR9I CCNGG 4 cut(s) 221, 222, 987, 1173
MvaI CCWGG 2 cut(s) 987, 1173
MvnI CGCG 1 cut(s) 391
MwoI GCNNNNNNNGC 4 cut(s) 49, 346, 781, 1186
NciI CCSGG 2 cut(s) 221, 222
NcoI CCATGG 1 cut(s) 46
NdeII GATC 4 cut(s) 847, 1106, 1280, 1314
NlaIII CATG 6 cut(s) 30, 50, 353, 632, 848, 1048
NlaIV GGNNCC 1 cut(s) 444
NspV TTCGAA 1 cut(s) 21
PagI TCATGA 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 789
PceI AGGCCT 1 cut(s) 960
PciSI GCTCTTC 1 cut(s) 158
PdmI GAANNNNTTC 2 cut(s) 152, 467
PfeI GAWTC 5 cut(s) 244, 386, 532, 1216, 1369
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 3 cut(s) 373, 1104, 1212
Ple19I CGATCG 1 cut(s) 1109
PleI GAGTC 1 cut(s) 1301
PpsI GAGTC 1 cut(s) 1301
Psp124BI GAGCTC 1 cut(s) 1191
Psp6I CCWGG 2 cut(s) 985, 1171
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 2 cut(s) 985, 1171
PspN4I GGNNCC 1 cut(s) 444
PspPI GGNCC 4 cut(s) 64, 327, 443, 719
PsuI RGATCY 1 cut(s) 1280
PvuI CGATCG 1 cut(s) 1109
PvuII CAGCTG 1 cut(s) 1213
RsaI GTAC 2 cut(s) 39, 931
RsaNI GTAC 2 cut(s) 38, 930
RseI CAYNNNNRTG 1 cut(s) 1043
Rsr2I CGGWCCG 1 cut(s) 64
RsrII CGGWCCG 1 cut(s) 64
SacI GAGCTC 1 cut(s) 1191
SalI GTCGAC 1 cut(s) 1353
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 4 cut(s) 92, 228, 1322, 1376
SatI GCNGC 3 cut(s) 372, 1103, 1211
Sau3AI GATC 4 cut(s) 847, 1106, 1280, 1314
Sau96I GGNCC 4 cut(s) 64, 327, 443, 719
SchI GAGTC 1 cut(s) 1301
ScrFI CCNGG 4 cut(s) 221, 222, 987, 1173
SduI GDGCHC 4 cut(s) 17, 572, 865, 1191
SfaNI GCATC 3 cut(s) 730, 1044, 1321
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 1 cut(s) 64
SmaI CCCGGG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 1043
SmlI CTYRAG 1 cut(s) 972
SmoI CTYRAG 1 cut(s) 972
Sse9I AATT 6 cut(s) 93, 164, 375, 691, 1131, 1391
SseBI AGGCCT 1 cut(s) 960
SsiI CCGC 8 cut(s) 62, 67, 113, 275, 281, 371, 1180, 1242
SspMI CTAG 3 cut(s) 600, 663, 681
SstI GAGCTC 1 cut(s) 1191
StuI AGGCCT 1 cut(s) 960
StyD4I CCNGG 4 cut(s) 219, 220, 985, 1171
StyI CCWWGG 2 cut(s) 46, 1092
TaaI ACNGT 3 cut(s) 459, 574, 885
TaqI TCGA 7 cut(s) 9, 21, 146, 156, 162, 1354, 1367
TasI AATT 6 cut(s) 93, 164, 375, 691, 1131, 1391
TatI WGTACW 1 cut(s) 929
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 5 cut(s) 244, 386, 532, 1216, 1369
Tru1I TTAA 4 cut(s) 92, 228, 1322, 1376
Tru9I TTAA 4 cut(s) 92, 228, 1322, 1376
TscAI CASTG 4 cut(s) 570, 577, 1080, 1206
TseI GCWGC 2 cut(s) 1102, 1210
TspDTI ATGAA 4 cut(s) 113, 468, 684, 1224
TspMI CCCGGG 1 cut(s) 220
TspRI CASTG 4 cut(s) 570, 577, 1080, 1206
VpaK11BI GGWCC 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 2 cut(s) 164, 1391
XbaI TCTAGA 1 cut(s) 680
XmaI CCCGGG 1 cut(s) 220
XmiI GTMKAC 3 cut(s) 559, 828, 1354
XmnI GAANNNNTTC 2 cut(s) 152, 467
XspI CTAG 3 cut(s) 600, 663, 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.