Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0009282

Physical Location & Seq

Genomic Coordinates Forward (+)

42049743 .. 42055031

Gene Structure 5289 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1305800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGGAAGCAGCAGCAGCTCCGTCCGATTCCCCCACAATAAAGCCGATAAACAAGGGCGTGGTGCACAGGATCTGCGCCGGCCAAGTGATTCTCGACCTCTCCGCCGCCGTGAAAGAATTGGTGGAAAACAGCCTCGACGCCGGCGCCACCAGCATCGAAATCGGCCTCAAAGACTACGGCAAAGAGTGGTTCCAGGTCGTCGACAATGGCTGCGGCATTTCGCCGAACAACTTCCGGGTCCTTGCCCTCAAGCATCACACCTCGAAGCTCGCCGGGTTCCCGGACCTCCAGTCCCTCACCACCTTCGGCTTCCGCGGCGAGGCCTTGAGCTCTCTCTGTGCTTTGGGGGATTTGACTGTGGAAACCAGGACCAAGAACGAGACCGTCGCCACGCACTTGACATTCGACCATTCGGGTTTGTTGGTCGCCGAGAAGAAGACCGCCCGGCAAGCGGGCACTACCGTCACGGTGAAGAACTTGTTTTCGAATTTACCAGTGCGGTGCAAGGAATTCAGCCGCAACATTCGGAAGGAGTATGGAAAGCTTGTTTCTTTATTGAATGCCTATGCTCTTATTGCTAAAGGCGTTCGGTTAGTCTGCACGAATGCTACTGGTAAAAATGCGAAATCTGTGGTACTTAAAACACAAGGGAGTGGTTCCCTTAAAGATAACATTGTCACACTGTTTGGATTGACCACGTTCAATTGCTTAGAACCAGTGAGTATATCTGTGTCTGAAAGTTGCAAGGTTGATGGTTTTCTGTCCAAGTCTGGACAGGGTAGTGGGCGAAATATGGGAGATAGACAGTACTTTTTTGTGAATGGTAGACCGGTGGATATGCCTAAAGTTACCAAGCTTGTGAATGAGTTGTATAGAAGAGCAAATTCCCAGCAGCACCCCATTGCAATAATGAATTTTACGGTTCCAACAAGAGCGTGTGATGTCAATGTAACTCCTGATAAAAGGAAAGTGTTCTTTTCTGATGAAAGTGCCATACTGGTTGCCCTTAGGGAGGGTTTACAGGAGATATATTCTTCAAGTAATGCTCATTACACTGTAAATAAAATTGAGGACCCTGCTAAGGAAGCAGATAGGTCAGAGTTGTGTTCTCCTCATCAAAGGTCTCATAAGTTTCTGAAACAATCGCCTACAGAGGATGTTGTTTCTGAAGAAGTAAGCATTGCCACTACAGGCGGTTTGCAGCAAACATATTCTCCAAGTGATGCTCCTTGTTCTATTAATAAAGTTGAGGAGGTGCCTACTAAGGAAGCAGGTAGCTCTGAGTCGTGTTTTCGTCGTCAACTGTCTCGTATGTTCCCGAAGCAATCATCTCCGGATGGCAGTGTTCCCGAAGAAATTCATGTTAAGGATCACCTTGCAGAAGGCAATGCTCGTCTGAAAGCTCTTGAAACTGAGTCTGAGTACACTGATGATGTAGAAGAGTTGAGCCTAGAAAATGCGATGAGCAAGGACTTTACTCTTAGAGTACACAGCAGTAAGAAGGTTGGTGGTAGTAGTAGCAAATTAACAACACATAACAACAGTATGACAACTGATCAAACTCGTTCTCTTTCAAGAGTGGTTGAGAATGCTGCCAATGGAGATTCATGTAGTCGTTCAATCTCTGTTCAATCATCACTCAATCAATTTGTGACCGTAAGTAAAAGAAAACATGAAAATATCAGTACAGTGCTATCTGAAATGCCTGTCCTAAGGAACCAAGCTCTTCATTCTCAATCCAAGGAAAGCACTTCTGATTTGCATGCTACTGTTTCAAACTCTCCGGTTCGACATGACCAGGCCAATGCTTCTTCTGAGATTGATAACTCTGCTGAAGTTGATGAGAATGAGCCATCCAAGTATCTTAGAGCAGACAAGATTCTCAATAAAATTAGAGGTCCAATTTCTGTTGGGGGCAACACTAAAGATCTCAATCCTAGTGAGGATGTACAGCCTCAACATATAGCAGATCCTCTTCCTATTATGGCATCACCTGCTTCACCTAGCGGAAGTCTAAATTGTTTGTCTGAAGATCTTCCTGTTGCAGCTCCATCACCTTCTTGTATACTATTAGATACTCCAAAGACTTCTTCTAGTGTTATGATGTGTTCCACCTTGCAATTTAGCTTTCAAGACCTAAAAACAAGGAGGCTGTGGATGTTGTCTAGATCGAAATCAAGCTTACCTGGAGGAGTAAAAGCACAAAGGTGCTTTGCTGCTGCGACGCTGGAGCTTTCTCAATCAGAAAACGAGGAGCGGAAAGCGAGGGCTTTAGCTGCAGCTACTACTGAGTTGGAAAGACTTTTTAGAAAGCAAGATTTTGGCAGAATGAAGGTAATTGGGCAATTCAATCTTGGCTTCATCATTGGGAAGTTAGATCAAGATCTGTTTATTGTGGATCAACATGCAGCTGATGAGAAGTACAATTTTGAGCGTCTATCACAATCTACTATCTTAAACCAACAGCCTCTTCTTCGGCCATTGAGGTTAGAGTTATCTCCTCAGGAAGAAGTTGTTGCTTCAATGCACATAGACATAATCAGGAAAAATGGATTCTCTTTGGAAGAGGATCCACAGGCTCCGCCTGGGCAACATTTTAACTTGAAAGCTGTCCCCTTCAGTAAAAACATAACTTTCGGAGTTGAAGATGTCAAGGACCTGATTTCCACTCTTGCTGATAGTCACGGGGAATGCTCAGTCATGGGGAGTTATAAGACGGACACCGTTGATTCTGTTTGCCCATCAAGAGTTCGTGCAATGCTAGCATCACGCGCATGCAGATCATCTGTTATGATTGGGGACGCCCTTGGAAGAAATGAGATGCAGAAGATTCTTGAGCATTTGGCGGGTCTGAAGTCTCCTTGGAATTGCCCGCATGGCAGGCCGACTATGCGCCATTTGGTCGACTTGAAAACCATACGCAGAAGGTCCGAAGAAGGCGATGATGAAGACTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005856 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009791 GO:0009987 GO:0010154 GO:0015630 GO:0016020 GO:0016445 GO:0016446 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0030983 GO:0031974 GO:0031981 GO:0032135 GO:0032138 GO:0032300 GO:0032389 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0061458 GO:0070013 GO:0071704 GO:0071944 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1990391 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03430 ko03460 map03430 map03460

Pfam Domains

Protein Families

DNA_mis_repair HATPase_c_3 MutL_C

Protein Analysis

986

Amino Acids

107.88

Weight (kDa)

6.81

Isoelectric Point (pI)

57.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c	PF02518	31 - 95	8.7e-06	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c_3	PF13589	34 - 136	3.7e-15	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
DNA_mis_repair	PF01119	227 - 344	8.9e-25	DNA mismatch repair protein, C-terminal domain
MutL_C	PF08676	780 - 936	1.2e-25	MutL C terminal dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0009282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02460 AT4G02460 AT4G02460 AT4G02460
fragaria_vesca	FvH4_2g25510 FvH4_2g25510 FvH4_2g25510 FvH4_2g25510 FvH4_2g25510
malus_domestica	MD11G1305800.v1.1
prunus_persica	Prupe.8G259200_v2.0.a1 Prupe.8G259200_v2.0.a1
pyrus_communis	pycom11g26760
rosa_chinensis	RchiOBHm_Chr6g0293721
rosa_laevigata	RLG00000011929
rosa_roxburghii	Rroxscaffold_7G00173950
rosa_rugosa	Rorug06G0237600
rosa_samantha	Rh6AG350400 Rh6BG357900 Rh6CG364700 Rh6DG351600
rosa_wichuraiana	Rw6G030510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2712
AarI	CACCTGC	1 cut(s)	2002
Acc36I	ACCTGC	2 cut(s)	1262, 2002
AccB1I	GGYRCC	2 cut(s)	143, 1255
AccBSI	CCGCTC	1 cut(s)	2257
AccI	GTMKAC	4 cut(s)	201, 826, 2065, 2904
AccII	CGCG	2 cut(s)	315, 2772
AccIII	TCCGGA	1 cut(s)	1333
AclWI	GGATC	6 cut(s)	77, 1377, 1964, 2406, 2564, 2577
AcoI	YGGCCR	2 cut(s)	79, 2477
AcsI	RAATTY	5 cut(s)	487, 509, 883, 913, 1356
AcuI	CTGAAG	5 cut(s)	1188, 1854, 2049, 2602, 2873
AcyI	GRCGYC	3 cut(s)	138, 144, 2802
AfaI	GTAC	7 cut(s)	636, 809, 1424, 1488, 1687, 1950, 2423
AfiI	CCNNNNNNNGG	4 cut(s)	319, 451, 1012, 1081
AgeI	ACCGGT	1 cut(s)	829
AhdI	GACNNNNNGTC	1 cut(s)	289
AjnI	CCWGG	5 cut(s)	192, 365, 1797, 2185, 2584
AjuI	GAANNNNNNNTTGG	4 cut(s)	2074, 2106, 2454, 2486
AleI	CACNNNNGTG	1 cut(s)	2206
Alw21I	GWGCWC	2 cut(s)	66, 332
Alw26I	GTCTC	4 cut(s)	374, 1128, 1311, 2862
Alw44I	GTGCAC	1 cut(s)	62
AlwI	GGATC	6 cut(s)	77, 1377, 1964, 2406, 2564, 2577
AlwNI	CAGNNNCTG	1 cut(s)	72
Aor13HI	TCCGGA	1 cut(s)	1333
AoxI	GGCC	6 cut(s)	79, 163, 321, 1800, 2477, 2882
ApaLI	GTGCAC	1 cut(s)	62
ApoI	RAATTY	5 cut(s)	487, 509, 883, 913, 1356
AseI	ATTAAT	1 cut(s)	1239
AsiGI	ACCGGT	1 cut(s)	829
Asp700I	GAANNNNTTC	4 cut(s)	230, 971, 1356, 2034
AspLEI	GCGC	4 cut(s)	77, 146, 2774, 2895
AspS9I	GGNCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
AsuC2I	CCSGG	4 cut(s)	236, 274, 281, 445
AsuHPI	GGTGA	6 cut(s)	289, 481, 1364, 1983, 1992, 2046
AsuII	TTCGAA	1 cut(s)	485
AsuNHI	GCTAGC	1 cut(s)	2761
AvaII	GGWCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
AxyI	CCTNAGG	3 cut(s)	1007, 1712, 2502
BaeGI	GKGCMC	2 cut(s)	66, 458
BamHI	GGATCC	1 cut(s)	2569
BanI	GGYRCC	2 cut(s)	143, 1255
BanII	GRGCYC	1 cut(s)	332
BbsI	GAAGAC	1 cut(s)	443
Bbv12I	GWGCWC	2 cut(s)	66, 332
BccI	CCATC	5 cut(s)	746, 1331, 1861, 2059, 2749
BceAI	ACGGC	2 cut(s)	92, 193
BcgI	CGANNNNNNTGC	2 cut(s)	142, 176
BciT130I	CCWGG	5 cut(s)	194, 367, 1799, 2187, 2586
BclI	TGATCA	1 cut(s)	1555
BcnI	CCSGG	4 cut(s)	236, 274, 281, 445
BcoDI	GTCTC	4 cut(s)	374, 1128, 1311, 2862
BfaI	CTAG	6 cut(s)	1451, 1938, 2004, 2094, 2166, 2762
BfmI	CTRYAG	3 cut(s)	1149, 1188, 2277
BfoI	RGCGCY	1 cut(s)	147
BfuAI	ACCTGC	2 cut(s)	1262, 2002
BglI	GCCNNNNNGGC	1 cut(s)	2877
BglII	AGATCT	3 cut(s)	1927, 2032, 2383
BmcAI	AGTACT	1 cut(s)	809
Bme1390I	CCNGG	9 cut(s)	194, 236, 274, 281, 367, 445, 1799, 2187, 2586
Bme18I	GGWCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
BmeRI	GACNNNNNGTC	1 cut(s)	289
BmgT120I	GGNCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
BmrFI	CCNGG	9 cut(s)	194, 236, 274, 281, 367, 445, 1799, 2187, 2586
BmsI	GCATC	6 cut(s)	162, 262, 1213, 1997, 2774, 2811
BmtI	GCTAGC	1 cut(s)	2765
BpiI	GAAGAC	1 cut(s)	443
BpmI	CTGGAG	3 cut(s)	272, 2208, 2249
Bpu10I	CCTNAGC	1 cut(s)	1080
Bpu14I	TTCGAA	1 cut(s)	485
BpuEI	CTTGAG	3 cut(s)	233, 346, 2855
BpuMI	CCSGG	4 cut(s)	236, 274, 281, 445
BsaBI	GATNNNNATC	3 cut(s)	1932, 2173, 2382
BsaHI	GRCGYC	3 cut(s)	138, 144, 2802
BsaI	GGTCTC	2 cut(s)	374, 1128
BsaJI	CCNNGG	5 cut(s)	313, 1740, 2585, 2806, 2861
BsaWI	WCCGGW	3 cut(s)	829, 1333, 1783
BsaXI	ACNNNNNCTCC	2 cut(s)	1198, 1228
Bsc4I	CCNNNNNNNGG	4 cut(s)	319, 451, 1012, 1081
Bse118I	RCCGGY	3 cut(s)	77, 140, 829
Bse1I	ACTGG	5 cut(s)	289, 494, 616, 716, 1002
Bse21I	CCTNAGG	3 cut(s)	1007, 1712, 2502
Bse3DI	GCAATG	4 cut(s)	900, 1179, 1393, 2763
Bse8I	GATNNNNATC	3 cut(s)	1932, 2173, 2382
BseAI	TCCGGA	1 cut(s)	1333
BseBI	CCWGG	5 cut(s)	194, 367, 1799, 2187, 2586
BseDI	CCNNGG	5 cut(s)	313, 1740, 2585, 2806, 2861
BseGI	GGATG	5 cut(s)	1162, 1342, 1853, 1951, 2163
BseJI	GATNNNNATC	3 cut(s)	1932, 2173, 2382
BseLI	CCNNNNNNNGG	4 cut(s)	319, 451, 1012, 1081
BseMI	GCAATG	4 cut(s)	900, 1179, 1393, 2763
BseMII	CTCAG	7 cut(s)	1272, 1404, 1410, 1806, 2280, 2516, 2709
BseNI	ACTGG	5 cut(s)	289, 494, 616, 716, 1002
BseRI	GAGGAG	5 cut(s)	1101, 1265, 2205, 2267, 2490
BseSI	GKGCMC	2 cut(s)	66, 458
BseYI	CCCAGC	1 cut(s)	888
BsgI	GTGCAG	1 cut(s)	583
Bsh1236I	CGCG	2 cut(s)	315, 2772
BshFI	GGCC	6 cut(s)	81, 165, 323, 1802, 2479, 2884
BshNI	GGYRCC	2 cut(s)	143, 1255
BshTI	ACCGGT	1 cut(s)	829
BsiHKAI	GWGCWC	2 cut(s)	66, 332
BsiSI	CCGG	9 cut(s)	78, 141, 235, 273, 281, 445, 830, 1334, 1784
BslFI	GGGAC	3 cut(s)	277, 2597, 2813
BslI	CCNNNNNNNGG	4 cut(s)	319, 451, 1012, 1081
BsmAI	GTCTC	4 cut(s)	374, 1128, 1311, 2862
BsmFI	GGGAC	3 cut(s)	277, 2597, 2813
BsmI	GAATGC	4 cut(s)	565, 610, 1594, 2696
BsnI	GGCC	6 cut(s)	81, 165, 323, 1802, 2479, 2884
Bso31I	GGTCTC	2 cut(s)	374, 1128
Bsp119I	TTCGAA	1 cut(s)	485
Bsp1286I	GDGCHC	3 cut(s)	66, 332, 458
Bsp13I	TCCGGA	1 cut(s)	1333
Bsp1407I	TGTACA	1 cut(s)	1948
BspANI	GGCC	6 cut(s)	81, 165, 323, 1802, 2479, 2884
BspCNI	CTCAG	7 cut(s)	1273, 1405, 1411, 1807, 2281, 2515, 2708
BspEI	TCCGGA	1 cut(s)	1333
BspFNI	CGCG	2 cut(s)	315, 2772
BspMAI	CTGCAG	1 cut(s)	2281
BspMI	ACCTGC	2 cut(s)	1262, 2002
BspOI	GCTAGC	1 cut(s)	2765
BspPI	GGATC	6 cut(s)	77, 1377, 1964, 2406, 2564, 2577
BspQI	GCTCTTC	2 cut(s)	871, 1731
BspT104I	TTCGAA	1 cut(s)	485
BspT107I	GGYRCC	2 cut(s)	143, 1255
BspTNI	GGTCTC	2 cut(s)	374, 1128
BsrBI	CCGCTC	1 cut(s)	2257
BsrDI	GCAATG	4 cut(s)	900, 1179, 1393, 2763
BsrFI	RCCGGY	3 cut(s)	77, 140, 829
BsrGI	TGTACA	1 cut(s)	1948
BsrI	ACTGG	5 cut(s)	289, 494, 616, 716, 1002
BssAI	RCCGGY	3 cut(s)	77, 140, 829
BssECI	CCNNGG	5 cut(s)	313, 1740, 2585, 2806, 2861
BssNAI	GTATAC	1 cut(s)	2066
BssNI	GRCGYC	3 cut(s)	138, 144, 2802
BssT1I	CCWWGG	3 cut(s)	1740, 2806, 2861
Bst1107I	GTATAC	1 cut(s)	2066
Bst2UI	CCWGG	5 cut(s)	194, 367, 1799, 2187, 2586
Bst6I	CTCTTC	6 cut(s)	871, 1434, 1731, 1980, 2475, 2559
BstACI	GRCGYC	3 cut(s)	138, 144, 2802
BstAPI	GCANNNNNTGC	1 cut(s)	1994
BstAUI	TGTACA	1 cut(s)	1948
BstBI	TTCGAA	1 cut(s)	485
BstDSI	CCRYGG	1 cut(s)	313
BstENI	CCTNNNNNAGG	1 cut(s)	1010
BstF5I	GGATG	5 cut(s)	1162, 1342, 1853, 1951, 2163
BstFNI	CGCG	2 cut(s)	315, 2772
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	4 cut(s)	77, 146, 2774, 2895
BstMAI	GTCTC	4 cut(s)	374, 1128, 1311, 2862
BstNI	CCWGG	5 cut(s)	194, 367, 1799, 2187, 2586
BstNSI	RCATGY	3 cut(s)	1766, 2408, 2778
BstSCI	CCNGG	9 cut(s)	192, 234, 272, 279, 365, 443, 1797, 2185, 2584
BstSFI	CTRYAG	3 cut(s)	1149, 1188, 2277
BstSLI	GKGCMC	2 cut(s)	66, 458
BstUI	CGCG	2 cut(s)	315, 2772
BstV2I	GAAGAC	1 cut(s)	443
BstX2I	RGATCY	6 cut(s)	69, 1927, 1969, 2032, 2383, 2569
BstYI	RGATCY	6 cut(s)	69, 1927, 1969, 2032, 2383, 2569
BstZ17I	GTATAC	1 cut(s)	2066
Bsu36I	CCTNAGG	3 cut(s)	1007, 1712, 2502
BsuRI	GGCC	6 cut(s)	81, 165, 323, 1802, 2479, 2884
BtgI	CCRYGG	1 cut(s)	313
BtgZI	GCGATG	1 cut(s)	1475
BtsCI	GGATG	5 cut(s)	1162, 1342, 1853, 1951, 2163
BtsI	GCAGTG	1 cut(s)	1348
BtsIMutI	CAGTG	7 cut(s)	501, 680, 723, 1053, 1348, 1425, 1695
BveI	ACCTGC	2 cut(s)	1262, 2002
CaiI	CAGNNNCTG	1 cut(s)	72
CfoI	GCGC	4 cut(s)	77, 146, 2774, 2895
Cfr10I	RCCGGY	3 cut(s)	77, 140, 829
Cfr13I	GGNCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
Cfr42I	CCGCGG	1 cut(s)	316
CseI	GACGC	4 cut(s)	146, 2233, 2423, 2810
Csp6I	GTAC	7 cut(s)	635, 808, 1423, 1487, 1686, 1949, 2422
CspAI	ACCGGT	1 cut(s)	829
CviAII	CATG	9 cut(s)	1361, 1608, 1673, 1763, 1793, 2405, 2701, 2775, 2876
CviQI	GTAC	7 cut(s)	635, 808, 1423, 1487, 1686, 1949, 2422
DinI	GGCGCC	1 cut(s)	145
DriI	GACNNNNNGTC	1 cut(s)	289
EaeI	YGGCCR	2 cut(s)	79, 2477
Eam1104I	CTCTTC	6 cut(s)	871, 1434, 1731, 1980, 2475, 2559
Eam1105I	GACNNNNNGTC	1 cut(s)	289
EarI	CTCTTC	6 cut(s)	871, 1434, 1731, 1980, 2475, 2559
EciI	GGCGGA	2 cut(s)	91, 2571
Ecl136II	GAGCTC	1 cut(s)	330
Eco130I	CCWWGG	3 cut(s)	1740, 2806, 2861
Eco147I	AGGCCT	1 cut(s)	323
Eco24I	GRGCYC	1 cut(s)	332
Eco31I	GGTCTC	2 cut(s)	374, 1128
Eco47I	GGWCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
Eco53kI	GAGCTC	1 cut(s)	330
Eco57I	CTGAAG	5 cut(s)	1188, 1854, 2049, 2602, 2873
Eco81I	CCTNAGG	3 cut(s)	1007, 1712, 2502
EcoICRI	GAGCTC	1 cut(s)	330
EcoNI	CCTNNNNNAGG	1 cut(s)	1010
EcoO109I	RGGNCCY	3 cut(s)	238, 1072, 2656
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	5 cut(s)	192, 365, 1797, 2185, 2584
EcoT14I	CCWWGG	3 cut(s)	1740, 2806, 2861
EcoT38I	GRGCYC	1 cut(s)	332
EgeI	GGCGCC	1 cut(s)	145
EheI	GGCGCC	1 cut(s)	145
ErhI	CCWWGG	3 cut(s)	1740, 2806, 2861
FaeI	CATG	9 cut(s)	1364, 1611, 1676, 1766, 1796, 2408, 2704, 2778, 2879
FaqI	GGGAC	3 cut(s)	277, 2597, 2813
FatI	CATG	9 cut(s)	1360, 1607, 1672, 1762, 1792, 2404, 2700, 2774, 2875
FauI	CCCGC	3 cut(s)	445, 2839, 2880
FbaI	TGATCA	1 cut(s)	1555
FblI	GTMKAC	4 cut(s)	201, 826, 2065, 2904
FokI	GGATG	5 cut(s)	1169, 1349, 1840, 1958, 2170
FriOI	GRGCYC	1 cut(s)	332
FspBI	CTAG	6 cut(s)	1451, 1938, 2004, 2094, 2166, 2762
GlaI	GCGC	4 cut(s)	76, 145, 2773, 2894
GsaI	CCCAGC	1 cut(s)	892
GsuI	CTGGAG	3 cut(s)	272, 2208, 2249
HaeII	RGCGCY	1 cut(s)	147
HaeIII	GGCC	6 cut(s)	81, 165, 323, 1802, 2479, 2884
HapII	CCGG	9 cut(s)	78, 141, 235, 273, 281, 445, 830, 1334, 1784
HgaI	GACGC	4 cut(s)	146, 2233, 2423, 2810
HhaI	GCGC	4 cut(s)	77, 146, 2774, 2895
Hin1I	GRCGYC	3 cut(s)	138, 144, 2802
Hin1II	CATG	9 cut(s)	1364, 1611, 1676, 1766, 1796, 2408, 2704, 2778, 2879
Hin6I	GCGC	4 cut(s)	75, 144, 2772, 2893
HinP1I	GCGC	4 cut(s)	75, 144, 2772, 2893
HincII	GTYRAC	3 cut(s)	202, 1301, 2905
HindII	GTYRAC	3 cut(s)	202, 1301, 2905
HindIII	AAGCTT	3 cut(s)	542, 854, 2179
HpaII	CCGG	9 cut(s)	78, 141, 235, 273, 281, 445, 830, 1334, 1784
HphI	GGTGA	6 cut(s)	289, 481, 1364, 1983, 1992, 2046
Hpy166II	GTNNAC	9 cut(s)	64, 202, 827, 1019, 1301, 1425, 1489, 2066, 2905
Hpy8I	GTNNAC	9 cut(s)	64, 202, 827, 1019, 1301, 1425, 1489, 2066, 2905
Hpy99I	CGWCG	5 cut(s)	140, 203, 389, 1299, 2227
HpyAV	CCTTC	9 cut(s)	313, 523, 1376, 1495, 2067, 2326, 2626, 2919, 2930
HpyCH4IV	ACGT	1 cut(s)	698
HpySE526I	ACGT	1 cut(s)	698
Hsp92I	GRCGYC	3 cut(s)	138, 144, 2802
Hsp92II	CATG	9 cut(s)	1364, 1611, 1676, 1766, 1796, 2408, 2704, 2778, 2879
HspAI	GCGC	4 cut(s)	75, 144, 2772, 2893
KasI	GGCGCC	1 cut(s)	143
Kpn2I	TCCGGA	1 cut(s)	1333
KroI	GCCGGC	2 cut(s)	77, 140
KroNI	GCCGGC	2 cut(s)	79, 142
Ksp22I	TGATCA	1 cut(s)	1555
KspI	CCGCGG	1 cut(s)	316
LguI	GCTCTTC	2 cut(s)	871, 1731
LmnI	GCTCC	6 cut(s)	22, 1231, 2053, 2230, 2254, 2584
LweI	GCATC	6 cut(s)	162, 262, 1213, 1997, 2774, 2811
MaeI	CTAG	6 cut(s)	1451, 1938, 2004, 2094, 2166, 2762
MaeII	ACGT	1 cut(s)	698
MaeIII	GTNAC	6 cut(s)	463, 676, 847, 949, 1651, 2681
MbiI	CCGCTC	1 cut(s)	2257
MfeI	CAATTG	1 cut(s)	703
MflI	RGATCY	6 cut(s)	69, 1927, 1969, 2032, 2383, 2569
MhlI	GDGCHC	3 cut(s)	66, 332, 458
Mly113I	GGCGCC	1 cut(s)	144
MlyI	GAGTC	3 cut(s)	1292, 1424, 2945
MmeI	TCCRAC	2 cut(s)	950, 2274
MreI	CGCCGGCG	1 cut(s)	140
MroI	TCCGGA	1 cut(s)	1333
MroNI	GCCGGC	2 cut(s)	77, 140
MroXI	GAANNNNTTC	4 cut(s)	230, 971, 1356, 2034
MseI	TTAA	7 cut(s)	639, 663, 1239, 1365, 1526, 2456, 2598
MslI	CAYNNNNRTG	2 cut(s)	2206, 2773
MspA1I	CMGCKG	2 cut(s)	315, 2411
MspI	CCGG	9 cut(s)	78, 141, 235, 273, 281, 445, 830, 1334, 1784
MspR9I	CCNGG	9 cut(s)	194, 236, 274, 281, 367, 445, 1799, 2187, 2586
MunI	CAATTG	1 cut(s)	703
Mva1269I	GAATGC	4 cut(s)	565, 610, 1594, 2696
MvaI	CCWGG	5 cut(s)	194, 367, 1799, 2187, 2586
MvnI	CGCG	2 cut(s)	315, 2772
NaeI	GCCGGC	2 cut(s)	79, 142
NarI	GGCGCC	1 cut(s)	144
NciI	CCSGG	4 cut(s)	236, 274, 281, 445
NgoMIV	GCCGGC	2 cut(s)	77, 140
NheI	GCTAGC	1 cut(s)	2761
NlaIII	CATG	9 cut(s)	1364, 1611, 1676, 1766, 1796, 2408, 2704, 2778, 2879
NmeAIII	GCCGAG	1 cut(s)	454
NmuCI	GTSAC	4 cut(s)	463, 676, 1651, 2681
NspI	RCATGY	3 cut(s)	1766, 2408, 2778
NspV	TTCGAA	1 cut(s)	485
OliI	CACNNNNGTG	1 cut(s)	2206
PaeI	GCATGC	2 cut(s)	1766, 2778
PaqCI	CACCTGC	1 cut(s)	2002
PceI	AGGCCT	1 cut(s)	323
PciSI	GCTCTTC	2 cut(s)	871, 1731
PctI	GAATGC	4 cut(s)	565, 610, 1594, 2696
PdiI	GCCGGC	2 cut(s)	79, 142
PdmI	GAANNNNTTC	4 cut(s)	230, 971, 1356, 2034
PfeI	GAWTC	7 cut(s)	26, 88, 1604, 1879, 2553, 2729, 2830
PfoI	TCCNGGA	1 cut(s)	279
PinAI	ACCGGT	1 cut(s)	829
PleI	GAGTC	3 cut(s)	1291, 1423, 2945
PluTI	GGCGCC	1 cut(s)	147
PpsI	GAGTC	3 cut(s)	1291, 1423, 2945
PpuMI	RGGWCCY	3 cut(s)	238, 1072, 2656
PshBI	ATTAAT	1 cut(s)	1239
PsiI	TTATAA	1 cut(s)	2712
Psp124BI	GAGCTC	1 cut(s)	332
Psp5II	RGGWCCY	3 cut(s)	238, 1072, 2656
Psp6I	CCWGG	5 cut(s)	192, 365, 1797, 2185, 2584
PspFI	CCCAGC	1 cut(s)	888
PspGI	CCWGG	5 cut(s)	192, 365, 1797, 2185, 2584
PspPI	GGNCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
PspPPI	RGGWCCY	3 cut(s)	238, 1072, 2656
PstI	CTGCAG	1 cut(s)	2281
PstNI	CAGNNNCTG	1 cut(s)	72
PsuI	RGATCY	6 cut(s)	69, 1927, 1969, 2032, 2383, 2569
PvuII	CAGCTG	1 cut(s)	2411
RsaI	GTAC	7 cut(s)	636, 809, 1424, 1488, 1687, 1950, 2423
RsaNI	GTAC	7 cut(s)	635, 808, 1423, 1487, 1686, 1949, 2422
RseI	CAYNNNNRTG	2 cut(s)	2206, 2773
SacI	GAGCTC	1 cut(s)	332
SacII	CCGCGG	1 cut(s)	316
SalI	GTCGAC	2 cut(s)	200, 2903
SapI	GCTCTTC	2 cut(s)	871, 1731
SaqAI	TTAA	7 cut(s)	639, 663, 1239, 1365, 1526, 2456, 2598
Sau96I	GGNCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
ScaI	AGTACT	1 cut(s)	809
SchI	GAGTC	3 cut(s)	1292, 1424, 2945
ScrFI	CCNGG	9 cut(s)	194, 236, 274, 281, 367, 445, 1799, 2187, 2586
SduI	GDGCHC	3 cut(s)	66, 332, 458
SfaNI	GCATC	6 cut(s)	162, 262, 1213, 1997, 2774, 2811
SfcI	CTRYAG	3 cut(s)	1149, 1188, 2277
SfoI	GGCGCC	1 cut(s)	145
Sfr303I	CCGCGG	1 cut(s)	316
SfuI	TTCGAA	1 cut(s)	485
SgrAI	CRCCGGYG	1 cut(s)	140
SgrBI	CCGCGG	1 cut(s)	316
SinI	GGWCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
SmiMI	CAYNNNNRTG	2 cut(s)	2206, 2773
SmlI	CTYRAG	3 cut(s)	248, 325, 2834
SmoI	CTYRAG	3 cut(s)	248, 325, 2834
SphI	GCATGC	2 cut(s)	1766, 2778
SseBI	AGGCCT	1 cut(s)	323
SspDI	GGCGCC	1 cut(s)	143
SspMI	CTAG	6 cut(s)	1451, 1938, 2004, 2094, 2166, 2762
SstI	GAGCTC	1 cut(s)	332
StuI	AGGCCT	1 cut(s)	323
StyD4I	CCNGG	9 cut(s)	192, 234, 272, 279, 365, 443, 1797, 2185, 2584
StyI	CCWWGG	3 cut(s)	1740, 2806, 2861
TaiI	ACGT	1 cut(s)	701
TatI	WGTACW	6 cut(s)	807, 1422, 1486, 1685, 1948, 2421
TauI	GCSGC	4 cut(s)	107, 216, 318, 519
TfiI	GAWTC	7 cut(s)	26, 88, 1604, 1879, 2553, 2729, 2830
Tru1I	TTAA	7 cut(s)	639, 663, 1239, 1365, 1526, 2456, 2598
Tru9I	TTAA	7 cut(s)	639, 663, 1239, 1365, 1526, 2456, 2598
TscAI	CASTG	7 cut(s)	501, 687, 723, 1060, 1348, 1432, 1695
TseFI	GTSAC	4 cut(s)	463, 676, 1651, 2681
Tsp45I	GTSAC	4 cut(s)	463, 676, 1651, 2681
TspDTI	ATGAA	8 cut(s)	926, 999, 1349, 1596, 1689, 1718, 2345, 2350
TspGWI	ACGGA	2 cut(s)	9, 2732
TspRI	CASTG	7 cut(s)	501, 687, 723, 1060, 1348, 1432, 1695
VneI	GTGCAC	1 cut(s)	62
VpaK11BI	GGWCC	7 cut(s)	238, 283, 369, 1072, 1898, 2656, 2928
VspI	ATTAAT	1 cut(s)	1239
XagI	CCTNNNNNAGG	1 cut(s)	1010
XapI	RAATTY	5 cut(s)	487, 509, 883, 913, 1356
XbaI	TCTAGA	1 cut(s)	2165
XceI	RCATGY	3 cut(s)	1766, 2408, 2778
XmiI	GTMKAC	4 cut(s)	201, 826, 2065, 2904
XmnI	GAANNNNTTC	4 cut(s)	230, 971, 1356, 2034
XspI	CTAG	6 cut(s)	1451, 1938, 2004, 2094, 2166, 2762
ZrmI	AGTACT	1 cut(s)	809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.