MD12G1003800.v1.1

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013134
Physical Location & Seq
Reverse (-)
550519 .. 554173
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1003800.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGTGGCGCAGCAGCTCCTGGTGGGACCCATCCCCATCAGCGGTGGCAGTGGCGGCCCCGGCGCCGGCGGCTTCTATGGAAATTTCAGTCATTTGTTTTCCCTGCCACCGTGTCATTTTCAACGCAGAAGAAGAGCCAGAACCCGTACTCGCCGGTGGACCCCACAATCGGCATCGCCAAGAGCCATGGTCAACGCCCAGCAGACCCACTACGCGGTGCTGTGCCTCCCACGCCATGCCACCGCCGCCGATATCAAGAGGGCTTATCGCCTTCTCGCTCGTAAGTATCATCCTGATGTTAGCAAAGATTCTCAAGCTGGAGAGGTGTTCAAGAGCATCCGTCAGGCGTATGAGGTTCTATCCAATGAAGCAACGAGGGCTCAATATGACCGGGAACTTAGATTACAAAAAGATACTGGTAGGCAACATAGTGGAAAATGGAACTACAGCACTGAATTTGAAGATGAGGTAAGGATCTATAAGTGGGCTGAAGTACGGCAGAGAATGCAACGGGAGAGATACTGGGAACGTTATAATGTTAATGAAGAGAGTTCGTCATATGGTAAAACTGATGGAGCAACTGAAGAAGGAGAGCCAGAGCAAGAGAGAGGTTCCTTCAGTGAAGTGCTTAGATCAGCCTTCGTATCTCTGTTTCTATTGCAGACATTTGGATCTCGGTTATCTCTCACGTTTAGCAGCCTGATGGCCTTGTTTGACAGGAAATTGGATGCCGGATACAAGATTGGTTATGTAATTGCTTGGGTTTTGGGTGGGAGAGGCGGGATCTTGCTGACTTTGTGTCTGTCGTTTGCAAGTTGGGTTTGTGGGAAAACCAGCAGCAATATGGTTGCTCTGGTGGTGGTAGCCATGTGGATCGGCTCCAATCTTGCAAAATTTGCACCGCTCCCGCAAGGTGCCCTGCTCACGCTTCTGTACATGTCTATTAAGCTACAAGTTGATTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

323

Amino Acids

36.34

Weight (kDa)

9.73

Isoelectric Point (pI)

47.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 69 - 130 3.6e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013134)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18140
fragaria_vesca FvH4_1g28130 FvH4_1g28130
malus_domestica MD12G1003800.v1.1
prunus_persica Prupe.7G004300_v2.0.a1
pyrus_communis pycom12g00320
rosa_chinensis RchiOBHm_Chr3g0497071
rosa_laevigata RLG00000022482
rosa_multiflora Rmu_co8330937.1_g000001 Rmu_sc0011396.1_g000005 Rmu_ssc0000003.1_g000038
rosa_roxburghii Rroxscaffold_6G00388410
rosa_rugosa Rorug03G0289400
rosa_samantha Rh3AG333900 Rh3BG371000 Rh3CG367900 Rh3DG368900
rosa_wichuraiana Rw3G029310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AccB1I GGYRCC 2 cut(s) 62, 914
AccBSI CCGCTC 1 cut(s) 904
AccII CGCG 1 cut(s) 215
AciI CCGC 9 cut(s) 42, 54, 69, 215, 243, 246, 780, 902, 908
AclI AACGTT 1 cut(s) 529
AclWI GGATC 4 cut(s) 482, 679, 791, 881
AcsI RAATTY 3 cut(s) 82, 455, 893
AcuI CTGAAG 3 cut(s) 510, 601, 603
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 148, 495, 935
AfiI CCNNNNNNNGG 4 cut(s) 41, 65, 169, 214
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 4 cut(s) 122, 331, 461, 964
AhdI GACNNNNNGTC 1 cut(s) 798
AjnI CCWGG 1 cut(s) 18
AluBI AGCT 3 cut(s) 16, 317, 949
AluI AGCT 3 cut(s) 16, 317, 949
AlwI GGATC 4 cut(s) 482, 679, 791, 881
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 2 cut(s) 55, 705
ApeKI GCWGC 4 cut(s) 10, 13, 696, 837
ApoI RAATTY 3 cut(s) 82, 455, 893
AspLEI GCGC 2 cut(s) 10, 65
AspS9I GGNCC 3 cut(s) 26, 56, 159
AsuC2I CCSGG 2 cut(s) 60, 392
AvaII GGWCC 2 cut(s) 26, 159
BaeGI GKGCMC 1 cut(s) 919
BanI GGYRCC 2 cut(s) 62, 914
BanII GRGCYC 1 cut(s) 382
BbvI GCAGC 4 cut(s) 22, 25, 708, 849
BccI CCATC 4 cut(s) 38, 44, 566, 697
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 1 cut(s) 20
BciVI GTATCC 1 cut(s) 728
BcnI CCSGG 2 cut(s) 60, 392
BfmI CTRYAG 1 cut(s) 445
BfoI RGCGCY 1 cut(s) 66
BfuI GTATCC 1 cut(s) 728
BisI GCNGC 7 cut(s) 11, 14, 55, 70, 246, 697, 838
BlsI GCNGC 7 cut(s) 12, 15, 56, 71, 247, 698, 839
Bme1390I CCNGG 3 cut(s) 20, 60, 392
Bme18I GGWCC 2 cut(s) 26, 159
BmeRI GACNNNNNGTC 1 cut(s) 798
BmgT120I GGNCC 3 cut(s) 26, 56, 159
BmiI GGNNCC 8 cut(s) 27, 28, 58, 64, 161, 613, 880, 916
BmrFI CCNGG 3 cut(s) 20, 60, 392
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 3 cut(s) 182, 345, 718
BmuI ACTGGG 1 cut(s) 532
BpmI CTGGAG 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 60, 392
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 2 cut(s) 58, 186
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 65, 169, 214
Bse118I RCCGGY 2 cut(s) 65, 153
Bse1I ACTGG 2 cut(s) 421, 527
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 2 cut(s) 58, 186
BseGI GGATG 4 cut(s) 30, 289, 336, 733
BseLI CCNNNNNNNGG 4 cut(s) 41, 65, 169, 214
BseNI ACTGG 2 cut(s) 421, 527
BseSI GKGCMC 1 cut(s) 919
BseXI GCAGC 4 cut(s) 22, 25, 708, 849
BseYI CCCAGC 1 cut(s) 198
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 57, 707
BshNI GGYRCC 2 cut(s) 62, 914
BsiSI CCGG 5 cut(s) 60, 66, 154, 391, 732
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 4 cut(s) 41, 65, 169, 214
BsmFI GGGAC 1 cut(s) 39
BsmI GAATGC 1 cut(s) 510
BsnI GGCC 2 cut(s) 57, 707
Bsp1286I GDGCHC 2 cut(s) 382, 919
Bsp1407I TGTACA 1 cut(s) 933
Bsp143I GATC 5 cut(s) 474, 632, 671, 783, 873
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 9 cut(s) 42, 54, 69, 215, 243, 246, 780, 902, 908
BspANI GGCC 2 cut(s) 57, 707
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 8 cut(s) 27, 28, 58, 64, 161, 613, 880, 916
BspPI GGATC 4 cut(s) 482, 679, 791, 881
BspQI GCTCTTC 1 cut(s) 127
BspT107I GGYRCC 2 cut(s) 62, 914
BsrBI CCGCTC 1 cut(s) 904
BsrFI RCCGGY 2 cut(s) 65, 153
BsrGI TGTACA 1 cut(s) 933
BsrI ACTGG 2 cut(s) 421, 527
BssAI RCCGGY 2 cut(s) 65, 153
BssECI CCNNGG 2 cut(s) 58, 186
BssMI GATC 5 cut(s) 474, 632, 671, 783, 873
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 111
Bst6I CTCTTC 2 cut(s) 127, 540
BstACI GRCGYC 1 cut(s) 63
BstAPI GCANNNNNTGC 2 cut(s) 505, 896
BstAUI TGTACA 1 cut(s) 933
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 398, 629
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 4 cut(s) 30, 289, 336, 733
BstFNI CGCG 1 cut(s) 215
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 2 cut(s) 10, 65
BstKTI GATC 5 cut(s) 477, 635, 674, 786, 876
BstMBI GATC 5 cut(s) 474, 632, 671, 783, 873
BstMWI GCNNNNNNNGC 8 cut(s) 54, 60, 69, 231, 245, 377, 505, 896
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 1 cut(s) 940
BstSCI CCNGG 3 cut(s) 18, 58, 390
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 919
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 22, 25, 708, 849
BstX2I RGATCY 3 cut(s) 474, 671, 783
BstYI RGATCY 3 cut(s) 474, 671, 783
BsuI GTATCC 1 cut(s) 728
BsuRI GGCC 2 cut(s) 57, 707
BtgI CCRYGG 1 cut(s) 186
BtgZI GCGATG 1 cut(s) 159
BtsCI GGATG 4 cut(s) 30, 289, 336, 733
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 3 cut(s) 55, 450, 625
Cac8I GCNNGC 1 cut(s) 67
CaiI CAGNNNCTG 1 cut(s) 19
CfoI GCGC 2 cut(s) 10, 65
Cfr10I RCCGGY 2 cut(s) 65, 153
Cfr13I GGNCC 3 cut(s) 26, 56, 159
Csp6I GTAC 3 cut(s) 147, 494, 934
CviAII CATG 4 cut(s) 187, 236, 868, 937
CviQI GTAC 3 cut(s) 147, 494, 934
DdeI CTNAG 2 cut(s) 398, 629
DinI GGCGCC 1 cut(s) 64
DpnI GATC 5 cut(s) 476, 634, 673, 785, 875
DpnII GATC 5 cut(s) 474, 632, 671, 783, 873
DriI GACNNNNNGTC 1 cut(s) 798
Eam1104I CTCTTC 2 cut(s) 127, 540
Eam1105I GACNNNNNGTC 1 cut(s) 798
EarI CTCTTC 2 cut(s) 127, 540
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 382
Eco32I GATATC 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 26, 159
Eco57I CTGAAG 3 cut(s) 510, 601, 603
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 18
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 382
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 4 cut(s) 190, 239, 871, 940
FaqI GGGAC 1 cut(s) 39
FatI CATG 4 cut(s) 186, 235, 867, 936
FauI CCCGC 2 cut(s) 773, 915
FauNDI CATATG 1 cut(s) 559
Fnu4HI GCNGC 7 cut(s) 11, 14, 55, 70, 246, 697, 838
FokI GGATG 4 cut(s) 17, 276, 323, 740
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 7 cut(s) 11, 14, 55, 70, 246, 697, 838
GlaI GCGC 2 cut(s) 9, 64
GluI GCNGC 7 cut(s) 11, 14, 55, 70, 246, 697, 838
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 2 cut(s) 57, 707
HapII CCGG 5 cut(s) 60, 66, 154, 391, 732
HhaI GCGC 2 cut(s) 10, 65
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 4 cut(s) 190, 239, 871, 940
Hin6I GCGC 2 cut(s) 8, 63
HinP1I GCGC 2 cut(s) 8, 63
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 1 cut(s) 308
HpaII CCGG 5 cut(s) 60, 66, 154, 391, 732
Hpy166II GTNNAC 2 cut(s) 159, 193
Hpy188III TCNNGA 3 cut(s) 256, 293, 331
Hpy8I GTNNAC 2 cut(s) 159, 193
HpyAV CCTTC 4 cut(s) 281, 581, 625, 649
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 2 cut(s) 529, 689
HpyCH4V TGCA 5 cut(s) 508, 661, 812, 890, 899
HpyF10VI GCNNNNNNNGC 8 cut(s) 54, 60, 69, 231, 245, 377, 505, 896
HpyF3I CTNAG 2 cut(s) 398, 629
HpySE526I ACGT 2 cut(s) 529, 689
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 4 cut(s) 190, 239, 871, 940
HspAI GCGC 2 cut(s) 8, 63
KasI GGCGCC 1 cut(s) 62
KflI GGGWCCC 1 cut(s) 26
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 5 cut(s) 474, 632, 671, 783, 873
LguI GCTCTTC 1 cut(s) 127
LmnI GCTCC 4 cut(s) 21, 575, 884, 909
Lsp1109I GCAGC 4 cut(s) 22, 25, 708, 849
LweI GCATC 3 cut(s) 182, 345, 718
MaeII ACGT 2 cut(s) 529, 689
MalI GATC 5 cut(s) 476, 634, 673, 785, 875
MbiI CCGCTC 1 cut(s) 904
MboI GATC 5 cut(s) 474, 632, 671, 783, 873
MboII GAAGA 5 cut(s) 141, 144, 473, 557, 596
MflI RGATCY 3 cut(s) 474, 671, 783
MhlI GDGCHC 2 cut(s) 382, 919
MluCI AATT 5 cut(s) 82, 455, 722, 753, 893
Mly113I GGCGCC 1 cut(s) 63
MnlI CCTC 8 cut(s) 236, 252, 316, 346, 369, 460, 602, 770
MreI CGCCGGCG 1 cut(s) 65
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 2 cut(s) 540, 945
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 5 cut(s) 60, 66, 154, 391, 732
MspR9I CCNGG 3 cut(s) 20, 60, 392
Mva1269I GAATGC 1 cut(s) 510
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 8 cut(s) 54, 60, 69, 231, 245, 377, 505, 896
NaeI GCCGGC 1 cut(s) 67
NarI GGCGCC 1 cut(s) 63
NciI CCSGG 2 cut(s) 60, 392
NcoI CCATGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 559
NdeII GATC 5 cut(s) 474, 632, 671, 783, 873
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 4 cut(s) 190, 239, 871, 940
NlaIV GGNNCC 8 cut(s) 27, 28, 58, 64, 161, 613, 880, 916
NspI RCATGY 1 cut(s) 940
PciI ACATGT 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 127
PctI GAATGC 1 cut(s) 510
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 7 cut(s) 12, 15, 56, 71, 247, 698, 839
PluTI GGCGCC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 26
PscI ACATGT 1 cut(s) 936
PsiI TTATAA 1 cut(s) 534
Psp1406I AACGTT 1 cut(s) 529
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 8 cut(s) 27, 28, 58, 64, 161, 613, 880, 916
PspPI GGNCC 3 cut(s) 26, 56, 159
PspPPI RGGWCCY 1 cut(s) 26
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 3 cut(s) 474, 671, 783
RsaI GTAC 3 cut(s) 148, 495, 935
RsaNI GTAC 3 cut(s) 147, 494, 934
RseI CAYNNNNRTG 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 2 cut(s) 540, 945
SatI GCNGC 7 cut(s) 11, 14, 55, 70, 246, 697, 838
Sau3AI GATC 5 cut(s) 474, 632, 671, 783, 873
Sau96I GGNCC 3 cut(s) 26, 56, 159
ScrFI CCNGG 3 cut(s) 20, 60, 392
SduI GDGCHC 2 cut(s) 382, 919
SfaNI GCATC 3 cut(s) 182, 345, 718
SfcI CTRYAG 1 cut(s) 445
SfoI GGCGCC 1 cut(s) 64
SgrAI CRCCGGYG 2 cut(s) 65, 153
SinI GGWCC 2 cut(s) 26, 159
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 312
SmoI CTYRAG 1 cut(s) 312
Sse9I AATT 5 cut(s) 82, 455, 722, 753, 893
SsiI CCGC 9 cut(s) 42, 54, 69, 215, 243, 246, 780, 902, 908
SspDI GGCGCC 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 18, 58, 390
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 2 cut(s) 532, 692
TasI AATT 5 cut(s) 82, 455, 722, 753, 893
TatI WGTACW 1 cut(s) 933
TauI GCSGC 3 cut(s) 57, 72, 248
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 540, 945
Tru9I TTAA 2 cut(s) 540, 945
TscAI CASTG 3 cut(s) 55, 457, 625
TseI GCWGC 4 cut(s) 10, 13, 696, 837
TspDTI ATGAA 2 cut(s) 381, 558
TspGWI ACGGA 1 cut(s) 329
TspRI CASTG 3 cut(s) 55, 457, 625
VpaK11BI GGWCC 2 cut(s) 26, 159
XapI RAATTY 3 cut(s) 82, 455, 893
XceI RCATGY 1 cut(s) 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.