MD12G1014600.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0018110
Physical Location & Seq
Forward (+)
1492183 .. 1496098
3916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1014600.v1.1.491

Sequence Viewer

Length: 471 bp
ATGTTTAACATCGCCGGGTGGGAAATGATGATATCTCTCGGTTTCATGGCTTCTGCAAGTGTACGAGTGTCAAATGAGTTTGGAAGAGGTAGCTCAAAGGCGGCAAAGTTCTCGATTGTAGTTATTGTGTCGATATCAGTCGTCATTGGATTGGCGCTTTTCGTGCTTTTCTTATTCCTAAGGGAACAACTTGCATACATTTTCACCAACGATGAGGCTGTGGCTAAAATGGTTTCTGAGTTGTCTCCTTTGTTGGCCTTCTCCATACTATTGAACAGCGTTCAACCCGTGCTCTCTGGAGTTGCTCTGGGAGCTGGATGGCAGAAATATGTAGCATATGTGAACCTAGGCTGCTATTACATCATAGGAATTCCTGTTGGAGTTGTGCTTGGTTATCCTCTAAAAATGCAAGTCAAAGGTGTTTGGATTGGAATGTTGTTTGGAACATTAGTCCAAACTAGTGTGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

157

Amino Acids

16.84

Weight (kDa)

9.13

Isoelectric Point (pI)

25.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 2 - 128 9.2e-25 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 101
AcsI RAATTY 1 cut(s) 369
AfaI GTAC 1 cut(s) 63
AgsI TTSAA 2 cut(s) 274, 284
AhlI ACTAGT 1 cut(s) 458
AluBI AGCT 2 cut(s) 93, 314
AluI AGCT 2 cut(s) 93, 314
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 249
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 351
ApoI RAATTY 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 346
AspLEI GCGC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 196
AvrII CCTAGG 1 cut(s) 346
AxyI CCTNAGG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 338
BccI CCATC 1 cut(s) 312
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 249
BcuI ACTAGT 1 cut(s) 458
BfaI CTAG 2 cut(s) 347, 459
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 102, 352
BlnI CCTAGG 1 cut(s) 346
BlsI GCNGC 2 cut(s) 103, 353
Bme1390I CCNGG 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 346
Bse21I CCTNAGG 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 338
BshFI GGCC 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 249
BsnI GGCC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 294
BspACI CCGC 1 cut(s) 101
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 79
BstDEI CTNAG 2 cut(s) 179, 237
BstF5I GGATG 1 cut(s) 323
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstMAI GTCTC 1 cut(s) 249
BstMWI GCNNNNNNNGC 2 cut(s) 163, 311
BstSCI CCNGG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 338
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 1 cut(s) 257
BtsCI GGATG 1 cut(s) 323
CfoI GCGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 62
CviAII CATG 1 cut(s) 46
CviJI RGCY 7 cut(s) 50, 93, 218, 224, 257, 314, 351
CviKI_1 RGCY 7 cut(s) 50, 93, 218, 224, 257, 314, 351
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 2 cut(s) 179, 237
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 346
Eco32I GATATC 2 cut(s) 33, 135
Eco81I CCTNAGG 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 369
EcoRV GATATC 2 cut(s) 33, 135
EcoT14I CCWWGG 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 1 cut(s) 49
FaiI YATR 8 cut(s) 47, 196, 266, 330, 337, 339, 365, 469
FatI CATG 1 cut(s) 45
FauNDI CATATG 1 cut(s) 337
Fnu4HI GCNGC 2 cut(s) 102, 352
FokI GGATG 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 102, 352
FspBI CTAG 2 cut(s) 347, 459
GlaI GCGC 1 cut(s) 156
GluI GCNGC 2 cut(s) 102, 352
GsuI CTGGAG 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 15
HhaI GCGC 1 cut(s) 157
Hin1II CATG 1 cut(s) 49
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HpaII CCGG 1 cut(s) 15
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 2 cut(s) 62, 343
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 2 cut(s) 112, 297
Hpy8I GTNNAC 2 cut(s) 62, 343
HpyAV CCTTC 1 cut(s) 268
HpyCH4V TGCA 3 cut(s) 56, 194, 409
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 311
HpyF3I CTNAG 2 cut(s) 179, 237
Hsp92II CATG 1 cut(s) 49
HspAI GCGC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 5 cut(s) 28, 282, 293, 300, 387
Lsp1109I GCAGC 1 cut(s) 338
MaeI CTAG 2 cut(s) 347, 459
MboII GAAGA 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 3 cut(s) 80, 208, 408
MseI TTAA 1 cut(s) 6
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 163, 311
NciI CCSGG 1 cut(s) 16
NdeI CATATG 1 cut(s) 337
NlaIII CATG 1 cut(s) 49
PkrI GCNGC 2 cut(s) 103, 353
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 2 cut(s) 102, 352
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 294
SetI ASST 5 cut(s) 91, 95, 316, 348, 421
SpeI ACTAGT 1 cut(s) 458
Sse9I AATT 1 cut(s) 369
SsiI CCGC 1 cut(s) 101
SspMI CTAG 2 cut(s) 347, 459
StyD4I CCNGG 1 cut(s) 14
StyI CCWWGG 1 cut(s) 346
TaqI TCGA 2 cut(s) 113, 131
TasI AATT 1 cut(s) 369
TauI GCSGC 1 cut(s) 104
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TseI GCWGC 1 cut(s) 351
TspDTI ATGAA 1 cut(s) 34
XapI RAATTY 1 cut(s) 369
XmaJI CCTAGG 1 cut(s) 346
XspI CTAG 2 cut(s) 347, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.