Rmu_sc0015572.1_g000004

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0015572.1
OG0018110
Physical Location & Seq
Reverse (-)
3386 .. 5360
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0015572.1_g000004.1.cds

Sequence Viewer

Length: 504 bp
atgatggcattttctgtcatcgtggtgactacgatctccttcatcatctccgtcatcgcagcaacagttgttcttatttttcgtgatgttatcagctacgccttcacggagggtgaagtcgtggccgctgctgtctcagatcttacccctcttcttgccctcaccctcctcctcaatggaattcagccagtattgtccggtgttgctgttgggtgtggatggcaagcttttgtggcgtatgtaaatgtgggctgttattactttgttggagtaccacttggtgcccttcttggattttacttcaactttggcgctaagggaatatggttagggatgatggcgggcacgttgatgcaaacaatcattctaatatggatcactgtccgagcagactggaagaaggagcctcgttcgctgccgcccacctgcgcccaccctgctcttagccagcctttgcgcacgctgcccgtgcagcccaacgcgctcccgctccttgctcgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

167

Amino Acids

17.91

Weight (kDa)

8.53

Isoelectric Point (pI)

27.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 434
Acc16I TGCGCA 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 434
AccB1I GGYRCC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 490
AccII CGCG 1 cut(s) 482
AciI CCGC 4 cut(s) 126, 341, 419, 488
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 1 cut(s) 273
AgsI TTSAA 1 cut(s) 304
AluBI AGCT 2 cut(s) 96, 227
AluI AGCT 2 cut(s) 96, 227
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 383
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 5 cut(s) 59, 128, 415, 463, 472
ApoI RAATTY 1 cut(s) 180
AspLEI GCGC 4 cut(s) 314, 431, 459, 484
AsuHPI GGTGA 3 cut(s) 37, 125, 154
BaeGI GKGCMC 2 cut(s) 286, 347
BanI GGYRCC 1 cut(s) 281
BbvI GCAGC 5 cut(s) 71, 115, 402, 450, 484
BccI CCATC 2 cut(s) 213, 331
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 1 cut(s) 502
BfoI RGCGCY 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 434
BglII AGATCT 1 cut(s) 139
BisI GCNGC 7 cut(s) 60, 126, 129, 416, 419, 464, 473
BlsI GCNGC 7 cut(s) 61, 127, 130, 417, 420, 465, 474
BmiI GGNNCC 2 cut(s) 283, 405
BmsI GCATC 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 188, 398
BseGI GGATG 2 cut(s) 224, 339
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 2 cut(s) 188, 398
BseRI GAGGAG 2 cut(s) 158, 161
BseSI GKGCMC 2 cut(s) 286, 347
BseXI GCAGC 5 cut(s) 71, 115, 402, 450, 484
BsgI GTGCAG 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 482
BshFI GGCC 1 cut(s) 125
BshNI GGYRCC 1 cut(s) 281
BsiSI CCGG 1 cut(s) 198
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 286, 347
Bsp143I GATC 3 cut(s) 33, 139, 375
BspACI CCGC 4 cut(s) 126, 341, 419, 488
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 149
BspFNI CGCG 1 cut(s) 482
BspLI GGNNCC 2 cut(s) 283, 405
BspMI ACCTGC 1 cut(s) 434
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 281
BsrBI CCGCTC 1 cut(s) 490
BsrI ACTGG 2 cut(s) 188, 398
BssMI GATC 3 cut(s) 33, 139, 375
Bst4CI ACNGT 2 cut(s) 67, 382
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 5 cut(s) 225, 343, 449, 461, 499
BstDEI CTNAG 3 cut(s) 136, 315, 443
BstF5I GGATG 2 cut(s) 224, 339
BstFNI CGCG 1 cut(s) 482
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 4 cut(s) 314, 431, 459, 484
BstKTI GATC 3 cut(s) 36, 142, 378
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 3 cut(s) 33, 139, 375
BstMWI GCNNNNNNNGC 7 cut(s) 233, 412, 437, 463, 469, 472, 481
BstSLI GKGCMC 2 cut(s) 286, 347
BstUI CGCG 1 cut(s) 482
BstV1I GCAGC 5 cut(s) 71, 115, 402, 450, 484
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 125
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 224, 339
BtsIMutI CAGTG 1 cut(s) 378
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 5 cut(s) 225, 343, 449, 461, 499
CfoI GCGC 4 cut(s) 314, 431, 459, 484
Csp6I GTAC 1 cut(s) 272
CviJI RGCY 9 cut(s) 96, 125, 187, 227, 252, 406, 447, 451, 475
CviKI_1 RGCY 9 cut(s) 96, 125, 187, 227, 252, 406, 447, 451, 475
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 3 cut(s) 136, 315, 443
DpnI GATC 3 cut(s) 35, 141, 377
DpnII GATC 3 cut(s) 33, 139, 375
DraIII CACNNNGTG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 180
FaiI YATR 3 cut(s) 240, 325, 373
FauI CCCGC 2 cut(s) 334, 495
Fnu4HI GCNGC 7 cut(s) 60, 126, 129, 416, 419, 464, 473
FokI GGATG 2 cut(s) 231, 346
Fsp4HI GCNGC 7 cut(s) 60, 126, 129, 416, 419, 464, 473
FspBI CTAG 1 cut(s) 502
FspI TGCGCA 1 cut(s) 458
GlaI GCGC 4 cut(s) 313, 430, 458, 483
GluI GCNGC 7 cut(s) 60, 126, 129, 416, 419, 464, 473
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 1 cut(s) 125
HapII CCGG 1 cut(s) 198
HhaI GCGC 4 cut(s) 314, 431, 459, 484
Hin6I GCGC 4 cut(s) 312, 429, 457, 482
HinP1I GCGC 4 cut(s) 312, 429, 457, 482
HindIII AAGCTT 1 cut(s) 225
HpaII CCGG 1 cut(s) 198
HphI GGTGA 3 cut(s) 37, 125, 154
Hpy188I TCNGA 2 cut(s) 139, 386
Hpy188III TCNNGA 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 49, 112, 296, 394
HpyCH4III ACNGT 2 cut(s) 67, 382
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 2 cut(s) 355, 472
HpyF10VI GCNNNNNNNGC 7 cut(s) 233, 412, 437, 463, 469, 472, 481
HpyF3I CTNAG 3 cut(s) 136, 315, 443
HpySE526I ACGT 1 cut(s) 347
HspAI GCGC 4 cut(s) 312, 429, 457, 482
Kzo9I GATC 3 cut(s) 33, 139, 375
LmnI GCTCC 3 cut(s) 403, 489, 495
LpnPI CCDG 6 cut(s) 201, 211, 379, 439, 450, 461
Lsp1109I GCAGC 5 cut(s) 71, 115, 402, 450, 484
LweI GCATC 1 cut(s) 342
MaeI CTAG 1 cut(s) 502
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 1 cut(s) 25
MalI GATC 3 cut(s) 35, 141, 377
MbiI CCGCTC 1 cut(s) 490
MboI GATC 3 cut(s) 33, 139, 375
MboII GAAGA 2 cut(s) 143, 409
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 2 cut(s) 286, 347
MluCI AATT 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 7 cut(s) 103, 159, 170, 176, 179, 182, 417
MslI CAYNNNNRTG 2 cut(s) 23, 350
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 198
MvnI CGCG 1 cut(s) 482
MwoI GCNNNNNNNGC 7 cut(s) 233, 412, 437, 463, 469, 472, 481
NdeII GATC 3 cut(s) 33, 139, 375
NlaIV GGNNCC 2 cut(s) 283, 405
NmuCI GTSAC 1 cut(s) 25
NsbI TGCGCA 1 cut(s) 458
PaqCI CACCTGC 1 cut(s) 434
PkrI GCNGC 7 cut(s) 61, 127, 130, 417, 420, 465, 474
PspN4I GGNNCC 2 cut(s) 283, 405
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
RseI CAYNNNNRTG 2 cut(s) 23, 350
SatI GCNGC 7 cut(s) 60, 126, 129, 416, 419, 464, 473
Sau3AI GATC 3 cut(s) 33, 139, 375
SduI GDGCHC 2 cut(s) 286, 347
SetI ASST 4 cut(s) 98, 229, 350, 428
SfaNI GCATC 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 23, 350
Sse9I AATT 1 cut(s) 180
SsiI CCGC 4 cut(s) 126, 341, 419, 488
SspMI CTAG 1 cut(s) 502
TaaI ACNGT 2 cut(s) 67, 382
TaiI ACGT 1 cut(s) 350
TasI AATT 1 cut(s) 180
TauI GCSGC 2 cut(s) 128, 421
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 5 cut(s) 59, 128, 415, 463, 472
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 1 cut(s) 31
TspGWI ACGGA 2 cut(s) 40, 122
TspRI CASTG 1 cut(s) 385
XapI RAATTY 1 cut(s) 180
XspI CTAG 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.