Rh2AG543300

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018110
Physical Location & Seq
Forward (+)
77184382 .. 77201936
17555 bp
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UTR
Exon/CDS
Intron
Rh2AG543300.1

Sequence Viewer

Length: 450 bp
ATGATCTCAGTTGGGTTAAATGCAGCTGCAAGTGTGCGAGTAAGCAATGAGCTTGGAGCCGAAAATCCCAAGTCCACATCATTTTCTGTCATCGTGGTGACTACGATCTCCTTCATCATCTCCGTCATTGCAGCAATCGTTGTTCTTATTTTTTGTGATGTTATCAGCTACGCCTTCACAGAGGGTGAAGTCGTGGCCGCTGCTGTCTCAGATCTTACCCCTCTTCGTGCCCTCACCCTCCTCCTCAATGGAATTCAGCTAGTCTTGTCCGGTGTTGCTGTTGGGTGTGGATGGCAAGCTTTTGTGGCGTATGTAAATGTGGGCTGTTATTACTTTGTTGGAGTACCACTTGGTGCCCTTCTTGGATTTTACTTCAACTTTGGCGCTAAGGTGGAAGAGGTAGCCAAAAGGTTGAACAAGTGGGACGAGAAGGAACCCGTAAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

149

Amino Acids

15.75

Weight (kDa)

5.02

Isoelectric Point (pI)

18.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 119 6.5e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 353
AciI CCGC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 2 cut(s) 376, 415
AluBI AGCT 5 cut(s) 26, 52, 168, 259, 299
AluI AGCT 5 cut(s) 26, 52, 168, 259, 299
Alw26I GTCTC 1 cut(s) 211
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 4 cut(s) 23, 26, 131, 200
ApoI RAATTY 1 cut(s) 252
AspLEI GCGC 1 cut(s) 386
AsuHPI GGTGA 3 cut(s) 109, 197, 226
BaeGI GKGCMC 2 cut(s) 232, 358
BanI GGYRCC 1 cut(s) 353
BbvI GCAGC 4 cut(s) 13, 35, 143, 187
BccI CCATC 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 387
BglII AGATCT 1 cut(s) 211
BisI GCNGC 5 cut(s) 24, 27, 132, 198, 201
BlsI GCNGC 5 cut(s) 25, 28, 133, 199, 202
BmiI GGNNCC 3 cut(s) 58, 355, 435
Bpu10I CCTNAGC 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse3DI GCAATG 2 cut(s) 52, 126
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMI GCAATG 2 cut(s) 52, 126
BseMII CTCAG 2 cut(s) 21, 222
BseRI GAGGAG 2 cut(s) 230, 233
BseSI GKGCMC 2 cut(s) 232, 358
BseXI GCAGC 4 cut(s) 13, 35, 143, 187
BshFI GGCC 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 353
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 232, 358
Bsp143I GATC 3 cut(s) 3, 105, 211
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 20, 221
BspLI GGNNCC 3 cut(s) 58, 355, 435
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 2 cut(s) 52, 126
BssMI GATC 3 cut(s) 3, 105, 211
Bst6I CTCTTC 2 cut(s) 228, 390
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 3 cut(s) 7, 208, 387
BstF5I GGATG 1 cut(s) 296
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 3 cut(s) 6, 108, 214
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 3 cut(s) 3, 105, 211
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstSLI GKGCMC 2 cut(s) 232, 358
BstV1I GCAGC 4 cut(s) 13, 35, 143, 187
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 297
CfoI GCGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 344
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 3 cut(s) 7, 208, 387
DpnI GATC 3 cut(s) 5, 107, 213
DpnII GATC 3 cut(s) 3, 105, 211
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 228, 390
EarI CTCTTC 2 cut(s) 228, 390
EcoRI GAATTC 1 cut(s) 252
FaiI YATR 1 cut(s) 312
FaqI GGGAC 1 cut(s) 437
Fnu4HI GCNGC 5 cut(s) 24, 27, 132, 198, 201
FokI GGATG 1 cut(s) 303
Fsp4HI GCNGC 5 cut(s) 24, 27, 132, 198, 201
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 385
GluI GCNGC 5 cut(s) 24, 27, 132, 198, 201
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 270
HhaI GCGC 1 cut(s) 386
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 297
HpaII CCGG 1 cut(s) 270
HphI GGTGA 3 cut(s) 109, 197, 226
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 211
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 4 cut(s) 121, 184, 368, 424
HpyCH4V TGCA 3 cut(s) 23, 29, 131
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 7, 208, 387
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 3 cut(s) 3, 105, 211
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 1 cut(s) 283
Lsp1109I GCAGC 4 cut(s) 13, 35, 143, 187
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 97
MalI GATC 3 cut(s) 5, 107, 213
MboI GATC 3 cut(s) 3, 105, 211
MboII GAAGA 2 cut(s) 215, 407
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 232, 358
MluCI AATT 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 7 cut(s) 175, 231, 242, 248, 251, 254, 391
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 26, 200
MspI CCGG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 3 cut(s) 3, 105, 211
NlaIV GGNNCC 3 cut(s) 58, 355, 435
NmuCI GTSAC 1 cut(s) 97
PkrI GCNGC 5 cut(s) 25, 28, 133, 199, 202
PspN4I GGNNCC 3 cut(s) 58, 355, 435
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 5 cut(s) 24, 27, 132, 198, 201
Sau3AI GATC 3 cut(s) 3, 105, 211
SduI GDGCHC 2 cut(s) 232, 358
SetI ASST 8 cut(s) 28, 54, 170, 261, 301, 393, 402, 413
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 1 cut(s) 252
SsiI CCGC 1 cut(s) 198
SspMI CTAG 1 cut(s) 260
TasI AATT 1 cut(s) 252
TauI GCSGC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 4 cut(s) 23, 26, 131, 200
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 1 cut(s) 112
XapI RAATTY 1 cut(s) 252
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.