Rh2CG526100

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018110
Physical Location & Seq
Forward (+)
70093681 .. 70094613
933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG526100.1

Sequence Viewer

Length: 531 bp
ATGGGTACGTGCAAGTATGCATGCAGTGTGCGAGTAAGCAATGAGCTTGGAGCCGGAAATCCCAAGTCCACAGCATTTTCTGTCATCGTGGTGACTACGATCTCCTTCATCATCTCCGTCATCGCAGCAATAGTTGTTCTTATTTTTCGTGATGTTATCAGCTACGCCTTCACGGAGGGTGAAGTCGTGGCCGCTGCTGTCTCAGATCTTACCCCTCTTCTTGCCCTCACCCTCCTCCTCAATGGAATTCAGCCTGTCTTGTCCGGTGTTGCTGTTGGGTGTGGATGGCAAGCTTTTGTGGCGTATGTAAATGTGGGCTGTTATTACTTTGTTGGAGTACCACTTGGTGCCCTTCTTGGATTTTACTTCAACTTTGGCGCTAAGGGAATATGGTTAGGGATGATGGCGGGCACGTTGACGCAAACAATCATTCTAATATGGATCACCGTTCGAACAGACTGGAAGAAGGAGGTGGAAGAGGCAGCCAAAAGGTTGAACAAGTGGGACGTGAAGGAACCCGTAAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

176

Amino Acids

18.89

Weight (kDa)

8.34

Isoelectric Point (pI)

21.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 6 - 117 4e-20 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 347
AciI CCGC 2 cut(s) 192, 407
AclWI GGATC 1 cut(s) 449
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 2 cut(s) 7, 339
AfiI CCNNNNNNNGG 1 cut(s) 524
AgsI TTSAA 2 cut(s) 370, 496
AjiI CACGTC 1 cut(s) 508
AluBI AGCT 3 cut(s) 46, 162, 293
AluI AGCT 3 cut(s) 46, 162, 293
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 449
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 3 cut(s) 125, 194, 482
ApoI RAATTY 1 cut(s) 246
AspLEI GCGC 1 cut(s) 380
AsuHPI GGTGA 4 cut(s) 103, 191, 220, 436
AsuII TTCGAA 1 cut(s) 451
BaeGI GKGCMC 2 cut(s) 352, 413
BanI GGYRCC 1 cut(s) 347
BbvI GCAGC 3 cut(s) 137, 181, 494
BccI CCATC 2 cut(s) 279, 397
BcoDI GTCTC 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 381
BglII AGATCT 1 cut(s) 205
BisI GCNGC 4 cut(s) 126, 192, 195, 483
BlsI GCNGC 4 cut(s) 127, 193, 196, 484
BmgBI CACGTC 1 cut(s) 508
BmiI GGNNCC 3 cut(s) 52, 349, 516
Bpu10I CCTNAGC 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 451
BsaAI YACGTR 1 cut(s) 9
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 46
BseGI GGATG 2 cut(s) 290, 405
BseLI CCNNNNNNNGG 1 cut(s) 524
BseMI GCAATG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 2 cut(s) 224, 227
BseSI GKGCMC 2 cut(s) 352, 413
BseXI GCAGC 3 cut(s) 137, 181, 494
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 347
BsiSI CCGG 2 cut(s) 54, 264
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 524
BsmAI GTCTC 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 352, 413
Bsp143I GATC 3 cut(s) 99, 205, 441
BspACI CCGC 2 cut(s) 192, 407
BspANI GGCC 1 cut(s) 191
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 3 cut(s) 52, 349, 516
BspPI GGATC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 451
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 46
BsrI ACTGG 1 cut(s) 464
BssMI GATC 3 cut(s) 99, 205, 441
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 222, 471
BstBAI YACGTR 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 3 cut(s) 22, 291, 409
BstDEI CTNAG 2 cut(s) 202, 381
BstF5I GGATG 2 cut(s) 290, 405
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 3 cut(s) 102, 208, 444
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 3 cut(s) 99, 205, 441
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNSI RCATGY 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 352, 413
BstV1I GCAGC 3 cut(s) 137, 181, 494
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 2 cut(s) 290, 405
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 3 cut(s) 22, 291, 409
CfoI GCGC 1 cut(s) 380
CseI GACGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 6, 338
CviAII CATG 1 cut(s) 21
CviJI RGCY 9 cut(s) 46, 53, 162, 191, 253, 293, 318, 485, 528
CviKI_1 RGCY 9 cut(s) 46, 53, 162, 191, 253, 293, 318, 485, 528
CviQI GTAC 2 cut(s) 6, 338
DdeI CTNAG 2 cut(s) 202, 381
DpnI GATC 3 cut(s) 101, 207, 443
DpnII GATC 3 cut(s) 99, 205, 441
DraIII CACNNNGTG 1 cut(s) 347
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 222, 471
EarI CTCTTC 2 cut(s) 222, 471
EcoRI GAATTC 1 cut(s) 246
EcoT22I ATGCAT 1 cut(s) 22
FaeI CATG 1 cut(s) 24
FaiI YATR 5 cut(s) 18, 22, 306, 391, 439
FaqI GGGAC 1 cut(s) 518
FatI CATG 1 cut(s) 20
FauI CCCGC 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 126, 192, 195, 483
FokI GGATG 2 cut(s) 297, 412
Fsp4HI GCNGC 4 cut(s) 126, 192, 195, 483
GlaI GCGC 1 cut(s) 379
GluI GCNGC 4 cut(s) 126, 192, 195, 483
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 1 cut(s) 191
HapII CCGG 2 cut(s) 54, 264
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 380
Hin1II CATG 1 cut(s) 24
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 291
HpaII CCGG 2 cut(s) 54, 264
HphI GGTGA 4 cut(s) 103, 191, 220, 436
Hpy166II GTNNAC 2 cut(s) 69, 417
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 1 cut(s) 149
Hpy8I GTNNAC 2 cut(s) 69, 417
HpyAV CCTTC 5 cut(s) 115, 178, 362, 460, 505
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 3 cut(s) 8, 413, 507
HpyCH4V TGCA 3 cut(s) 12, 20, 24
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 2 cut(s) 202, 381
HpySE526I ACGT 3 cut(s) 8, 413, 507
Hsp92II CATG 1 cut(s) 24
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 3 cut(s) 99, 205, 441
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 4 cut(s) 67, 267, 277, 445
Lsp1109I GCAGC 3 cut(s) 137, 181, 494
MaeII ACGT 3 cut(s) 8, 413, 507
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 101, 207, 443
MboI GATC 3 cut(s) 99, 205, 441
MboII GAAGA 3 cut(s) 209, 475, 488
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 352, 413
MluCI AATT 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 313
MnlI CCTC 8 cut(s) 169, 225, 236, 242, 245, 248, 463, 472
Mph1103I ATGCAT 1 cut(s) 22
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 54, 264
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeII GATC 3 cut(s) 99, 205, 441
NlaIII CATG 1 cut(s) 24
NlaIV GGNNCC 3 cut(s) 52, 349, 516
NmuCI GTSAC 1 cut(s) 91
NsiI ATGCAT 1 cut(s) 22
NspI RCATGY 1 cut(s) 24
NspV TTCGAA 1 cut(s) 451
PaeI GCATGC 1 cut(s) 24
PkrI GCNGC 4 cut(s) 127, 193, 196, 484
Ppu21I YACGTR 1 cut(s) 9
PspN4I GGNNCC 3 cut(s) 52, 349, 516
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 2 cut(s) 7, 339
RsaNI GTAC 2 cut(s) 6, 338
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 4 cut(s) 126, 192, 195, 483
Sau3AI GATC 3 cut(s) 99, 205, 441
SduI GDGCHC 2 cut(s) 352, 413
SetI ASST 8 cut(s) 11, 48, 164, 295, 416, 474, 494, 510
SfuI TTCGAA 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 89
SphI GCATGC 1 cut(s) 24
Sse9I AATT 1 cut(s) 246
SsiI CCGC 2 cut(s) 192, 407
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 3 cut(s) 11, 416, 510
TaqI TCGA 1 cut(s) 451
TasI AATT 1 cut(s) 246
TauI GCSGC 1 cut(s) 194
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 3 cut(s) 125, 194, 482
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 1 cut(s) 97
TspGWI ACGGA 2 cut(s) 106, 188
TspRI CASTG 1 cut(s) 31
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.