RLG00000022607

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018110
Physical Location & Seq
Forward (+)
5132478 .. 5133539
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022607

Sequence Viewer

Length: 513 bp
ATGATGATATCCCTGGGCTTCTTAGCTGCTGCAAGTGTACGAGTGTCAAATGAACTTGGAAGAGGTAGCTCAGAAGGGGCAAAGTTCTCAATCGTGGTTATAGTGATCACATCATTTGCCATTGGTTTTGTGCTTTTCTTGTTCTTCCTGTTCTTGAGGGAAAGTCTTGCCTACATTTTCACTGACAATGAAGAGCTAGCTGAAGCCTTCGCCGATTTGTCACCTCTATTGGCTTTCTCCATATTGCTGAACAGTGTTCAACCTGTGTTCTCTGGAGTTGCTGTTGGTGCTGGACGGCAGAAAATTGTAGCATATGTCAATATAGCATGCTACTACTTGATAGGGATTCCTGTAGGAGTTGTGCTTGGTTATGTTGTAAACCTACTAGTCAAAACTATTTTACTAGTCATATTTACCTACAAAACTGATTGGGATAAACAGGTATCCATAGCTCGTAACCATGTTAACAACTGGGTGGTAACTGACAATCCGGAATCGAGCCCCGACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

171

Amino Acids

18.53

Weight (kDa)

5.02

Isoelectric Point (pI)

29.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 120 8.6e-24 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 490
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 1 cut(s) 39
AgsI TTSAA 1 cut(s) 260
AhlI ACTAGT 2 cut(s) 385, 403
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 5 cut(s) 26, 69, 196, 200, 452
AluI AGCT 5 cut(s) 26, 69, 196, 200, 452
Aor13HI TCCGGA 1 cut(s) 490
ApeKI GCWGC 2 cut(s) 26, 29
AsuHPI GGTGA 1 cut(s) 213
AsuNHI GCTAGC 1 cut(s) 196
BanII GRGCYC 1 cut(s) 503
BbvI GCAGC 2 cut(s) 13, 16
BceAI ACGGC 1 cut(s) 311
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 1 cut(s) 105
BcuI ACTAGT 2 cut(s) 385, 403
BfaI CTAG 3 cut(s) 197, 386, 404
BfmI CTRYAG 1 cut(s) 351
BfuI GTATCC 1 cut(s) 454
BisI GCNGC 2 cut(s) 27, 30
BlsI GCNGC 2 cut(s) 28, 31
Bme1390I CCNGG 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 14
BmrI ACTGGG 1 cut(s) 481
BmtI GCTAGC 1 cut(s) 200
BmuI ACTGGG 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 12, 13
BsaWI WCCGGW 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bse1I ACTGG 1 cut(s) 476
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 12, 13
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 476
BseXI GCAGC 2 cut(s) 13, 16
BsiSI CCGG 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 503
Bsp13I TCCGGA 1 cut(s) 490
Bsp143I GATC 1 cut(s) 105
BspCNI CTCAG 1 cut(s) 83
BspEI TCCGGA 1 cut(s) 490
BspOI GCTAGC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 186
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 12, 13
BssMI GATC 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 2 cut(s) 55, 186
BstC8I GCNNGC 2 cut(s) 198, 328
BstDEI CTNAG 2 cut(s) 22, 70
BstKTI GATC 1 cut(s) 108
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 12
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 13, 16
BsuI GTATCC 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 180, 259
Cac8I GCNNGC 2 cut(s) 198, 328
Csp6I GTAC 1 cut(s) 38
CviAII CATG 2 cut(s) 327, 461
CviJI RGCY 9 cut(s) 18, 26, 69, 196, 200, 206, 233, 452, 501
CviKI_1 RGCY 9 cut(s) 18, 26, 69, 196, 200, 206, 233, 452, 501
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 2 cut(s) 22, 70
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
Eam1104I CTCTTC 2 cut(s) 55, 186
EarI CTCTTC 2 cut(s) 55, 186
Eco24I GRGCYC 1 cut(s) 503
Eco32I GATATC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 222
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 503
FaeI CATG 2 cut(s) 330, 464
FatI CATG 2 cut(s) 326, 460
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 2 cut(s) 27, 30
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 2 cut(s) 27, 30
FspBI CTAG 3 cut(s) 197, 386, 404
GluI GCNGC 2 cut(s) 27, 30
GsuI CTGGAG 1 cut(s) 294
HapII CCGG 1 cut(s) 491
Hin1II CATG 2 cut(s) 330, 464
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 2 cut(s) 346, 494
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 1 cut(s) 491
HphI GGTGA 1 cut(s) 213
Hpy166II GTNNAC 3 cut(s) 38, 379, 466
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 154, 273, 491
Hpy8I GTNNAC 3 cut(s) 38, 379, 466
HpyAV CCTTC 2 cut(s) 68, 217
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 2 cut(s) 22, 70
Hsp92II CATG 2 cut(s) 330, 464
Kpn2I TCCGGA 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 105
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 1 cut(s) 105
LguI GCTCTTC 1 cut(s) 186
LpnPI CCDG 9 cut(s) 26, 161, 258, 276, 276, 363, 425, 457, 504
Lsp1109I GCAGC 2 cut(s) 13, 16
MaeI CTAG 3 cut(s) 197, 386, 404
MaeIII GTNAC 3 cut(s) 219, 455, 478
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 3 cut(s) 72, 136, 203
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 1 cut(s) 303
MnlI CCTC 3 cut(s) 56, 150, 234
MroI TCCGGA 1 cut(s) 490
MseI TTAA 1 cut(s) 465
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 287
NdeI CATATG 1 cut(s) 313
NdeII GATC 1 cut(s) 105
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 330, 464
NmuCI GTSAC 1 cut(s) 219
NspI RCATGY 1 cut(s) 330
PaeI GCATGC 1 cut(s) 330
PasI CCCWGGG 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 346, 494
PkrI GCNGC 2 cut(s) 28, 31
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 2 cut(s) 27, 30
Sau3AI GATC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 503
SfcI CTRYAG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SpeI ACTAGT 2 cut(s) 385, 403
SphI GCATGC 1 cut(s) 330
Sse9I AATT 1 cut(s) 303
SspMI CTAG 3 cut(s) 197, 386, 404
StyD4I CCNGG 1 cut(s) 12
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 1 cut(s) 497
TasI AATT 1 cut(s) 303
TfiI GAWTC 2 cut(s) 346, 494
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 2 cut(s) 187, 259
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 2 cut(s) 26, 29
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 2 cut(s) 66, 204
TspRI CASTG 2 cut(s) 187, 259
XceI RCATGY 1 cut(s) 330
XspI CTAG 3 cut(s) 197, 386, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.