Rroxscaffold_4G00278050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018110
Physical Location & Seq
Reverse (-)
1204722 .. 1209928
5207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00278050.1

Sequence Viewer

Length: 447 bp
ATGTCAAGACCGGCACTGAATTTTCTTATACTTCGTCTCATTCTAATTCTAGAGCAGACAACCTTATATTGTACTGAAGCTGATGTCTCACAAGCTTCAGGAGAAAACAATGAGTTTCACCCCAGAAAGGAGATGCGGCACTCCTCCCGGGTAGACACAACCAGGCCCAACTCGGGTAGGCCCCAAGGCCCAATCCGGATGAGCCCAACCCAAACGCAAGAGAACTCCGGGCTCGCTACCATCGGAACCTCCGTAAGCCGCCCCTCGAACCTCCACCAAGACCCGCCATCGATTGCCGATCAAGAGCAAAACCACCAGCCCAACCCTCCGCAGCCCATCCAAGCCTCTTTTTCCGCATCCACGACCCACCACACTTTACATCAACCAACCCCCGGAACACTCACCGACGACCCGATTGAGGATTCGATCCGACATCGCATCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.47

Weight (kDa)

6.28

Isoelectric Point (pI)

59.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 195
AciI CCGC 5 cut(s) 136, 259, 284, 329, 354
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 2 cut(s) 81, 96
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 4 cut(s) 127, 173, 392, 418
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 2 cut(s) 80, 95
AluI AGCT 2 cut(s) 80, 95
Alw26I GTCTC 2 cut(s) 41, 91
AlwI GGATC 1 cut(s) 421
Ama87I CYCGRG 2 cut(s) 147, 172
Aor13HI TCCGGA 1 cut(s) 195
AoxI GGCC 3 cut(s) 164, 179, 187
ApeKI GCWGC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 19
AspS9I GGNCC 3 cut(s) 165, 180, 188
AsuC2I CCSGG 4 cut(s) 148, 149, 229, 393
AsuHPI GGTGA 2 cut(s) 110, 394
AvaI CYCGRG 2 cut(s) 147, 172
BanII GRGCYC 2 cut(s) 206, 234
BbvI GCAGC 1 cut(s) 343
BccI CCATC 3 cut(s) 248, 295, 344
BciT130I CCWGG 1 cut(s) 163
BcnI CCSGG 4 cut(s) 148, 149, 229, 393
BcoDI GTCTC 2 cut(s) 41, 91
BfaI CTAG 2 cut(s) 50, 445
BisI GCNGC 3 cut(s) 137, 259, 332
BlsI GCNGC 3 cut(s) 138, 260, 333
Bme1390I CCNGG 5 cut(s) 148, 149, 163, 229, 393
BmeT110I CYCGRG 2 cut(s) 147, 172
BmgT120I GGNCC 3 cut(s) 165, 180, 188
BmiI GGNNCC 2 cut(s) 182, 247
BmrFI CCNGG 5 cut(s) 148, 149, 163, 229, 393
BmsI GCATC 2 cut(s) 123, 365
BpuMI CCSGG 4 cut(s) 148, 149, 229, 393
Bsa29I ATCGAT 1 cut(s) 290
BsaJI CCNNGG 3 cut(s) 147, 184, 391
BsaWI WCCGGW 1 cut(s) 195
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 173, 392, 418
Bse118I RCCGGY 1 cut(s) 10
BseAI TCCGGA 1 cut(s) 195
BseBI CCWGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 290
BseDI CCNNGG 3 cut(s) 147, 184, 391
BseGI GGATG 4 cut(s) 204, 336, 356, 438
BseLI CCNNNNNNNGG 4 cut(s) 127, 173, 392, 418
BseRI GAGGAG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 343
BshFI GGCC 3 cut(s) 166, 181, 189
BshVI ATCGAT 1 cut(s) 290
BsiHKCI CYCGRG 2 cut(s) 147, 172
BsiSI CCGG 5 cut(s) 11, 148, 196, 228, 393
BslI CCNNNNNNNGG 4 cut(s) 127, 173, 392, 418
BsmAI GTCTC 2 cut(s) 41, 91
BsmBI CGTCTC 1 cut(s) 41
BsnI GGCC 3 cut(s) 166, 181, 189
BsoBI CYCGRG 2 cut(s) 147, 172
Bsp1286I GDGCHC 2 cut(s) 206, 234
Bsp13I TCCGGA 1 cut(s) 195
Bsp143I GATC 2 cut(s) 298, 426
BspACI CCGC 5 cut(s) 136, 259, 284, 329, 354
BspANI GGCC 3 cut(s) 166, 181, 189
BspDI ATCGAT 1 cut(s) 290
BspEI TCCGGA 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 182, 247
BspPI GGATC 1 cut(s) 421
BsrFI RCCGGY 1 cut(s) 10
BssAI RCCGGY 1 cut(s) 10
BssECI CCNNGG 3 cut(s) 147, 184, 391
BssMI GATC 2 cut(s) 298, 426
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 234
BstF5I GGATG 4 cut(s) 204, 336, 356, 438
BstKTI GATC 2 cut(s) 301, 429
BstMAI GTCTC 2 cut(s) 41, 91
BstMBI GATC 2 cut(s) 298, 426
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 5 cut(s) 146, 147, 161, 227, 391
BstV1I GCAGC 1 cut(s) 343
Bsu15I ATCGAT 1 cut(s) 290
BsuRI GGCC 3 cut(s) 166, 181, 189
BsuTUI ATCGAT 1 cut(s) 290
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 4 cut(s) 204, 336, 356, 438
BtsIMutI CAGTG 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 10
Cfr13I GGNCC 3 cut(s) 165, 180, 188
Cfr9I CCCGGG 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 290
Csp6I GTAC 1 cut(s) 72
CviQI GTAC 1 cut(s) 72
DpnI GATC 2 cut(s) 300, 428
DpnII GATC 2 cut(s) 298, 426
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 2 cut(s) 206, 234
Eco57I CTGAAG 2 cut(s) 81, 96
Eco88I CYCGRG 2 cut(s) 147, 172
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 2 cut(s) 206, 234
ErhI CCWWGG 1 cut(s) 184
Esp3I CGTCTC 1 cut(s) 41
FaiI YATR 2 cut(s) 29, 67
FauI CCCGC 1 cut(s) 291
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 137, 259, 332
FokI GGATG 4 cut(s) 211, 323, 343, 425
FriOI GRGCYC 2 cut(s) 206, 234
Fsp4HI GCNGC 3 cut(s) 137, 259, 332
FspBI CTAG 2 cut(s) 50, 445
GluI GCNGC 3 cut(s) 137, 259, 332
HaeIII GGCC 3 cut(s) 166, 181, 189
HapII CCGG 5 cut(s) 11, 148, 196, 228, 393
HindIII AAGCTT 1 cut(s) 93
HinfI GANTC 1 cut(s) 422
HpaII CCGG 5 cut(s) 11, 148, 196, 228, 393
HphI GGTGA 2 cut(s) 110, 394
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 2 cut(s) 245, 431
Hpy188III TCNNGA 5 cut(s) 6, 50, 99, 196, 302
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 410
Kpn2I TCCGGA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 298, 426
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 2 cut(s) 123, 365
MaeI CTAG 2 cut(s) 50, 445
MalI GATC 2 cut(s) 300, 428
MboI GATC 2 cut(s) 298, 426
MhlI GDGCHC 2 cut(s) 206, 234
MluCI AATT 2 cut(s) 19, 45
MnlI CCTC 7 cut(s) 154, 259, 274, 281, 336, 355, 412
MroI TCCGGA 1 cut(s) 195
MspI CCGG 5 cut(s) 11, 148, 196, 228, 393
MspR9I CCNGG 5 cut(s) 148, 149, 163, 229, 393
MvaI CCWGG 1 cut(s) 163
NciI CCSGG 4 cut(s) 148, 149, 229, 393
NdeII GATC 2 cut(s) 298, 426
NlaIV GGNNCC 2 cut(s) 182, 247
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 1 cut(s) 422
PkrI GCNGC 3 cut(s) 138, 260, 333
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 182, 247
PspPI GGNCC 3 cut(s) 165, 180, 188
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
SatI GCNGC 3 cut(s) 137, 259, 332
Sau3AI GATC 2 cut(s) 298, 426
Sau96I GGNCC 3 cut(s) 165, 180, 188
ScrFI CCNGG 5 cut(s) 148, 149, 163, 229, 393
SduI GDGCHC 2 cut(s) 206, 234
SetI ASST 5 cut(s) 65, 82, 97, 251, 273
SfaNI GCATC 2 cut(s) 123, 365
SmaI CCCGGG 1 cut(s) 149
Sse9I AATT 2 cut(s) 19, 45
SsiI CCGC 5 cut(s) 136, 259, 284, 329, 354
SspMI CTAG 2 cut(s) 50, 445
StyD4I CCNGG 5 cut(s) 146, 147, 161, 227, 391
StyI CCWWGG 1 cut(s) 184
TaqI TCGA 3 cut(s) 266, 290, 425
TasI AATT 2 cut(s) 19, 45
TatI WGTACW 1 cut(s) 71
TauI GCSGC 2 cut(s) 139, 261
TfiI GAWTC 1 cut(s) 422
TscAI CASTG 1 cut(s) 21
TseI GCWGC 1 cut(s) 331
TspGWI ACGGA 1 cut(s) 241
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 1 cut(s) 21
XapI RAATTY 1 cut(s) 19
XbaI TCTAGA 1 cut(s) 49
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 2 cut(s) 50, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.