Rh2CG525300

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018110
Physical Location & Seq
Forward (+)
70020661 .. 70021852
1192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG525300.1

Sequence Viewer

Length: 537 bp
ATGATCTCAATTGGGTTAAATGCAGCTGCAAGTGTGCGAGTAAGCAATGAGCTTGGAGCCGGAAATCCCAAGTCCACAGCATTTTCTGTCATCGTGGTGACTACGATCTCCTTCATCATCTCCGTCATCGCAGCAATAGTTGTTCTTATTTTTCGTGATGTTATCAGCTACACCTTCACGGAGGGTGAAGTCGTGGCCGCTGCTGTCTCAGATCTTACCCCTCTTCTTGCCCTCACCCTCCTCCTCAATGGAATTCAGCCAGTATTGTCCGGTGTTGCTGTTGGGTGTGGATGGCAAGCTTTTGTGGCGTATGTAAATGTGGGCTGTTATTACTTTGTTGGAGTACCACTTGGTGCCCTTCTTGGATTTTACTTCAACTTTGGCGCTAAGGGAATATGGTCAGGGATGATGGCGGGCACGTTGATGCAAACAATCATTCTAATATGGATCACCGTTCGAACAGACTGGAAGAAGGAGGTGGAAGAGGCAGCCAAAAGGTTGAACAAGTGGGACGTGAAGGAACCCGTAAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

178

Amino Acids

19.01

Weight (kDa)

7.72

Isoelectric Point (pI)

16.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 119 1.1e-21 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 353
AciI CCGC 2 cut(s) 198, 413
AclWI GGATC 1 cut(s) 455
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 2 cut(s) 376, 502
AjiI CACGTC 1 cut(s) 514
AluBI AGCT 4 cut(s) 26, 52, 168, 299
AluI AGCT 4 cut(s) 26, 52, 168, 299
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 1 cut(s) 455
AoxI GGCC 1 cut(s) 195
ApeKI GCWGC 5 cut(s) 23, 26, 131, 200, 488
ApoI RAATTY 1 cut(s) 252
AspLEI GCGC 1 cut(s) 386
AsuHPI GGTGA 4 cut(s) 109, 197, 226, 442
AsuII TTCGAA 1 cut(s) 457
BaeGI GKGCMC 2 cut(s) 358, 419
BanI GGYRCC 1 cut(s) 353
BbvI GCAGC 5 cut(s) 13, 35, 143, 187, 500
BccI CCATC 2 cut(s) 285, 403
BcoDI GTCTC 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 387
BglII AGATCT 1 cut(s) 211
BisI GCNGC 6 cut(s) 24, 27, 132, 198, 201, 489
BlsI GCNGC 6 cut(s) 25, 28, 133, 199, 202, 490
BmgBI CACGTC 1 cut(s) 514
BmiI GGNNCC 3 cut(s) 58, 355, 522
BmsI GCATC 1 cut(s) 414
Bpu10I CCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 457
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 260, 470
Bse3DI GCAATG 1 cut(s) 52
BseGI GGATG 2 cut(s) 296, 411
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 260, 470
BseRI GAGGAG 2 cut(s) 230, 233
BseSI GKGCMC 2 cut(s) 358, 419
BseXI GCAGC 5 cut(s) 13, 35, 143, 187, 500
BshFI GGCC 1 cut(s) 197
BshNI GGYRCC 1 cut(s) 353
BsiSI CCGG 2 cut(s) 60, 270
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 1 cut(s) 197
Bsp119I TTCGAA 1 cut(s) 457
Bsp1286I GDGCHC 2 cut(s) 358, 419
Bsp143I GATC 4 cut(s) 3, 105, 211, 447
BspACI CCGC 2 cut(s) 198, 413
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 3 cut(s) 58, 355, 522
BspPI GGATC 1 cut(s) 455
BspT104I TTCGAA 1 cut(s) 457
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 52
BsrI ACTGG 2 cut(s) 260, 470
BssMI GATC 4 cut(s) 3, 105, 211, 447
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 2 cut(s) 228, 477
BstBI TTCGAA 1 cut(s) 457
BstC8I GCNNGC 2 cut(s) 297, 415
BstDEI CTNAG 2 cut(s) 208, 387
BstF5I GGATG 2 cut(s) 296, 411
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 4 cut(s) 6, 108, 214, 450
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 4 cut(s) 3, 105, 211, 447
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstSLI GKGCMC 2 cut(s) 358, 419
BstV1I GCAGC 5 cut(s) 13, 35, 143, 187, 500
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 1 cut(s) 197
BtgZI GCGATG 1 cut(s) 112
BtrI CACGTC 1 cut(s) 514
BtsCI GGATG 2 cut(s) 296, 411
Cac8I GCNNGC 2 cut(s) 297, 415
CfoI GCGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 344
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 2 cut(s) 208, 387
DpnI GATC 4 cut(s) 5, 107, 213, 449
DpnII GATC 4 cut(s) 3, 105, 211, 447
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 228, 477
EarI CTCTTC 2 cut(s) 228, 477
EcoRI GAATTC 1 cut(s) 252
FaiI YATR 3 cut(s) 312, 397, 445
FaqI GGGAC 1 cut(s) 524
FauI CCCGC 1 cut(s) 406
Fnu4HI GCNGC 6 cut(s) 24, 27, 132, 198, 201, 489
FokI GGATG 2 cut(s) 303, 418
Fsp4HI GCNGC 6 cut(s) 24, 27, 132, 198, 201, 489
GlaI GCGC 1 cut(s) 385
GluI GCNGC 6 cut(s) 24, 27, 132, 198, 201, 489
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 1 cut(s) 197
HapII CCGG 2 cut(s) 60, 270
HhaI GCGC 1 cut(s) 386
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HindIII AAGCTT 1 cut(s) 297
HpaII CCGG 2 cut(s) 60, 270
HphI GGTGA 4 cut(s) 109, 197, 226, 442
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 5 cut(s) 121, 184, 368, 466, 511
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 2 cut(s) 419, 513
HpyCH4V TGCA 3 cut(s) 23, 29, 427
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 2 cut(s) 208, 387
HpySE526I ACGT 2 cut(s) 419, 513
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 4 cut(s) 3, 105, 211, 447
LmnI GCTCC 1 cut(s) 56
LpnPI CCDG 5 cut(s) 73, 273, 283, 387, 451
Lsp1109I GCAGC 5 cut(s) 13, 35, 143, 187, 500
LweI GCATC 1 cut(s) 414
MaeII ACGT 2 cut(s) 419, 513
MaeIII GTNAC 1 cut(s) 97
MalI GATC 4 cut(s) 5, 107, 213, 449
MboI GATC 4 cut(s) 3, 105, 211, 447
MboII GAAGA 3 cut(s) 215, 481, 494
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 358, 419
MluCI AATT 2 cut(s) 9, 252
MmeI TCCRAC 1 cut(s) 319
MnlI CCTC 8 cut(s) 175, 231, 242, 248, 251, 254, 469, 478
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 2 cut(s) 95, 422
MspA1I CMGCKG 2 cut(s) 26, 200
MspI CCGG 2 cut(s) 60, 270
MunI CAATTG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 4 cut(s) 3, 105, 211, 447
NlaIV GGNNCC 3 cut(s) 58, 355, 522
NmuCI GTSAC 1 cut(s) 97
NspV TTCGAA 1 cut(s) 457
PkrI GCNGC 6 cut(s) 25, 28, 133, 199, 202, 490
PspN4I GGNNCC 3 cut(s) 58, 355, 522
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 2 cut(s) 95, 422
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 6 cut(s) 24, 27, 132, 198, 201, 489
Sau3AI GATC 4 cut(s) 3, 105, 211, 447
SduI GDGCHC 2 cut(s) 358, 419
SetI ASST 9 cut(s) 28, 54, 170, 176, 301, 422, 480, 500, 516
SfaNI GCATC 1 cut(s) 414
SfuI TTCGAA 1 cut(s) 457
SmiMI CAYNNNNRTG 2 cut(s) 95, 422
Sse9I AATT 2 cut(s) 9, 252
SsiI CCGC 2 cut(s) 198, 413
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 2 cut(s) 422, 516
TaqI TCGA 1 cut(s) 457
TasI AATT 2 cut(s) 9, 252
TauI GCSGC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 5 cut(s) 23, 26, 131, 200, 488
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 2 cut(s) 112, 194
XapI RAATTY 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.