RLG00000031132

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018110
Physical Location & Seq
Forward (+)
2471508 .. 2473220
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031132

Sequence Viewer

Length: 552 bp
ATGACCATATCTGGATGGGTGTTCATGGTTGCTGTGGGTTTCAATGCTGCTGCAAGTGTTAGGATTGGGAATGAGCTCGGGGCGGGACACCCAAAGTCGGCCGCATTTGCAGTAATAATAGTGACATCAACCTCGTTCCTCATCTCTGTAGTGTGTGCCATAGTTGTGCTTGCACTACGAAATGTCATAAGCTACGCGTTCACCGAGGGCGAGACTGTGTCCAATGCAGTTTCAGCCCTATCTCCTTACCTTGCTGTCTCTATTATGCTCAACGGCATCCAACCTGTTTTGTCTGGCCTAACTCTGGGTAAGCCCCAAGGCCCAATCCAGATGAGCCCAGCCCGAACGCAGGAGAACTCCAGCCTCTGCTACCATCGGAACCTCTGTAAGCCGCCCCCCAGAACCTCCACCAAGACTGCCATCAGTTGCCGATCAAGAGCAAAACCACCAGCCCAACCCTCTGCAGCCATCGAAGCCTCTTTTTCCAGATCCACAGCCCACCACACTTTACATCAACCAACCCCTGGAACAGCACACCGACGACCCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

184

Amino Acids

19.23

Weight (kDa)

10.05

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 100 1.9e-17 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018110)

Species Orthologous Gene IDs
malus_domestica MD12G1014600.v1.1
pyrus_communis pycom09g03040
rosa_laevigata RLG00000022607 RLG00000031132
rosa_multiflora Rmu_co8050928.1_g000001 Rmu_sc0015572.1_g000004
rosa_roxburghii Rroxscaffold_4G00278050
rosa_samantha Rh2AG543300 Rh2CG525300 Rh2CG526100 Rh5DG543300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 524
AccII CGCG 1 cut(s) 197
AciI CCGC 3 cut(s) 83, 102, 392
AclWI GGATC 1 cut(s) 483
AcoI YGGCCR 1 cut(s) 99
AfiI CCNNNNNNNGG 4 cut(s) 97, 304, 349, 524
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 1 cut(s) 43
AjnI CCWGG 1 cut(s) 523
AluBI AGCT 2 cut(s) 76, 192
AluI AGCT 2 cut(s) 76, 192
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 2 cut(s) 206, 262
AlwI GGATC 1 cut(s) 483
AlwNI CAGNNNCTG 1 cut(s) 366
Ama87I CYCGRG 1 cut(s) 77
AoxI GGCC 3 cut(s) 99, 295, 319
ApeKI GCWGC 3 cut(s) 47, 50, 464
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 77
BanII GRGCYC 2 cut(s) 78, 338
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 34, 37, 476
BccI CCATC 4 cut(s) 9, 381, 428, 476
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 206, 262
BfmI CTRYAG 2 cut(s) 147, 462
BisI GCNGC 5 cut(s) 48, 51, 102, 392, 465
BlsI GCNGC 5 cut(s) 49, 52, 103, 393, 466
Bme1390I CCNGG 1 cut(s) 525
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 1 cut(s) 285
BpmI CTGGAG 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 204, 316, 523
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 304, 349, 524
BseBI CCWGG 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 204, 316, 523
BseGI GGATG 2 cut(s) 20, 276
BseLI CCNNNNNNNGG 4 cut(s) 97, 304, 349, 524
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 3 cut(s) 34, 37, 476
BseYI CCCAGC 1 cut(s) 337
Bsh1236I CGCG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 3 cut(s) 101, 297, 321
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 4 cut(s) 97, 304, 349, 524
BsmAI GTCTC 2 cut(s) 206, 262
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 3 cut(s) 101, 297, 321
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 2 cut(s) 78, 338
Bsp143I GATC 2 cut(s) 431, 488
BspACI CCGC 3 cut(s) 83, 102, 392
BspANI GGCC 3 cut(s) 101, 297, 321
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 466
BspPI GGATC 1 cut(s) 483
BssECI CCNNGG 3 cut(s) 204, 316, 523
BssMI GATC 2 cut(s) 431, 488
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 217
BstC8I GCNNGC 1 cut(s) 171
BstF5I GGATG 2 cut(s) 20, 276
BstFNI CGCG 1 cut(s) 197
BstKTI GATC 2 cut(s) 434, 491
BstMAI GTCTC 2 cut(s) 206, 262
BstMBI GATC 2 cut(s) 431, 488
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 3 cut(s) 107, 233, 473
BstNI CCWGG 1 cut(s) 525
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 2 cut(s) 147, 462
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 3 cut(s) 34, 37, 476
BstX2I RGATCY 1 cut(s) 488
BstYI RGATCY 1 cut(s) 488
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 3 cut(s) 101, 297, 321
BtsCI GGATG 2 cut(s) 20, 276
Cac8I GCNNGC 1 cut(s) 171
CaiI CAGNNNCTG 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 320
CviAII CATG 1 cut(s) 25
DpnI GATC 2 cut(s) 433, 490
DpnII GATC 2 cut(s) 431, 488
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 76
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 78, 338
Eco52I CGGCCG 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 77
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 523
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 78, 338
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 1 cut(s) 28
FaiI YATR 5 cut(s) 8, 26, 161, 188, 266
FaqI GGGAC 1 cut(s) 99
FatI CATG 1 cut(s) 24
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 5 cut(s) 48, 51, 102, 392, 465
FokI GGATG 2 cut(s) 27, 263
FriOI GRGCYC 2 cut(s) 78, 338
Fsp4HI GCNGC 5 cut(s) 48, 51, 102, 392, 465
GluI GCNGC 5 cut(s) 48, 51, 102, 392, 465
GsaI CCCAGC 1 cut(s) 341
GsuI CTGGAG 1 cut(s) 343
HaeIII GGCC 3 cut(s) 101, 297, 321
Hin1II CATG 1 cut(s) 28
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 4 cut(s) 12, 328, 435, 486
Hpy8I GTNNAC 1 cut(s) 201
Hpy99I CGWCG 1 cut(s) 543
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 5 cut(s) 53, 110, 173, 227, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 233, 473
Hsp92II CATG 1 cut(s) 28
Kzo9I GATC 2 cut(s) 431, 488
Lsp1109I GCAGC 3 cut(s) 34, 37, 476
LweI GCATC 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 433, 490
MboI GATC 2 cut(s) 431, 488
MflI RGATCY 1 cut(s) 488
MhlI GDGCHC 2 cut(s) 78, 338
MluI ACGCGT 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 8 cut(s) 142, 149, 199, 374, 392, 415, 469, 487
MslI CAYNNNNRTG 1 cut(s) 164
MspR9I CCNGG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 525
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 107, 233, 473
NdeII GATC 2 cut(s) 431, 488
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PflFI GACNNNGTC 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 5 cut(s) 49, 52, 103, 393, 466
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 1 cut(s) 523
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 488
PsyI GACNNNGTC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 78
SatI GCNGC 5 cut(s) 48, 51, 102, 392, 465
Sau3AI GATC 2 cut(s) 431, 488
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 2 cut(s) 78, 338
SetI ASST 7 cut(s) 78, 134, 194, 252, 286, 384, 407
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 2 cut(s) 147, 462
SmiMI CAYNNNNRTG 1 cut(s) 164
SsiI CCGC 3 cut(s) 83, 102, 392
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 217
TaqI TCGA 1 cut(s) 471
TauI GCSGC 2 cut(s) 104, 394
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 3 cut(s) 47, 50, 464
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 1 cut(s) 13
Tth111I GACNNNGTC 1 cut(s) 217
Van91I CCANNNNNTGG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.