MD12G1022200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010508
Physical Location & Seq
Forward (+)
2209896 .. 2212980
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1022200.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGGAGGTGGAAACTCAGGCTCAGAGCCTCCCAGCCGCCTTCGACAACCCTTCCTTTTCTTACTCCCACAACGACCTCGGTTCCCGCGACTTCCGAAGCTCTGAAATGCCCCGAGTTGACCCGAAATGCGTTGCCTCCGACGTGCTGGCTCCGTTGGAACACAACGGGGCGAAGGCGAGTGAGACGGACGCGCCGGGCGGCGAGTGGTCGGAGTCGACTGCCGCGGTGAAGGTTCAGAAGGTGTATCGGAGTTACCGCACCCGGCGCAGGTTAGCCGACTCCGCCGTCGTCGTCGAAGAGTACTGGTGGCAAGCGATAGATGCTTGGAGATTGAACCGCAGTACGATTTCGTTCTTTGATCTTGGAATTGAATCTGCCATTAAACGATGGAACCGTGTTGGCAAGAATGCTTCTAAGGTGGGCAAGGGGCTGTCCAAAGACGCCAAAGCACAGAAATTGGCTTTTCAGCATTGGATTGAAGCTATTGATCCAAGGCATAGGTATGGTCATAGCCTGCATGTGTATTATGAGGAGTGGTCTAAAGCAGATGCTGGTCAGCCATTTTTTTACTGGTTGGATGTAGGAGATGGAAAAGATATTGACCTGAAAGAATGCCCGAGATCGAAGCTTCGACAACAATGCATCAAGTATCTTGGACCTCAAGAGAGAATGCATTACGAGTACATTGTTGAGGAAGGGAGACTTATTCACTCGCAAACCGAAGAGCTTCTTGATACAAAGGATGGATCGCTGGGTGCTAAGTGGATATTCGTTATGAGCACATCTAAGAAACTCTATGCTGGCTTGAAAAGGAAAGGGGTTTTCCATCATTCTAGCTTCTTGGCTGGAGGAGCAACGATAGCCGCTGGAAGGCTAGAGGCAGAGAAAGGAATTCTGAAGTCAATTTCTGCATATAGCGGGCATTACCGGCCAACAGATGACAGGCTTGATACATTTTTATCAGTTCTCAAGGAAAACGGGGTGAACCTTGATGAAGTTAAGATACGCAAGGCAAGTGACGATTCTGATAGCTACGATGATGGCAAATCTAATGGCGGGACTACATTTAAAATGCCAACCAACTTTGAAACTCCTCAACTGGAGATTCTAGGCGAAGTTGAGAAAACCCAATCTCCAGAACCAACTGAACTTCCCCAACCGGAAGCCAAACCTGATTACCAAAGGACTTTATCCGGAGGTCTTCAGAGTCCAAGACCTGAGGTGCCTAAGAATAAGATACTGGAGAGGATCAACTCCAAGACCTCAGCGAAATCATACCAACTCGGGCATCAGCTATCACTCAAGTGGTCAACAGGAGCTGGCCCGAGAATTGGTTGTGTTGCTGACTACCCAATAGAGCTGAGAACGCAGGCCTTGGAGTTTGTTAACCTATCTCCGAAGTCTCCCCCAACGCCATCCTACAGGCGGTTCCCTTGTCTCGCTTCACCTACATCTGGTCTCGCATCACCTACTTCTGGTCTCGTGTCACCTACATCTGGTTTCGCATCACCTACATTGAAGTCCACCTCGGATGTCAATAACGGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

517

Amino Acids

57.35

Weight (kDa)

8.34

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 1222
AccI GTMKAC 1 cut(s) 215
AccII CGCG 3 cut(s) 87, 191, 224
AccIII TCCGGA 1 cut(s) 1193
AclWI GGATC 3 cut(s) 482, 754, 1256
AcoI YGGCCR 1 cut(s) 929
AcsI RAATTY 1 cut(s) 891
AcuI CTGAAG 2 cut(s) 917, 1187
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 3 cut(s) 302, 343, 683
AfiI CCNNNNNNNGG 7 cut(s) 267, 870, 1331, 1425, 1454, 1475, 1496
AgsI TTSAA 6 cut(s) 334, 371, 479, 808, 1088, 1519
AjiI CACGTC 1 cut(s) 142
AleI CACNNNNGTG 1 cut(s) 1303
AluBI AGCT 9 cut(s) 99, 482, 628, 727, 837, 1032, 1294, 1319, 1360
AluI AGCT 9 cut(s) 99, 482, 628, 727, 837, 1032, 1294, 1319, 1360
Alw21I GWGCWC 1 cut(s) 782
Alw26I GTCTC 6 cut(s) 176, 694, 1407, 1442, 1463, 1484
AlwI GGATC 3 cut(s) 482, 754, 1256
AlwNI CAGNNNCTG 2 cut(s) 551, 1319
Ama87I CYCGRG 4 cut(s) 111, 616, 1283, 1324
Aor13HI TCCGGA 1 cut(s) 1193
AoxI GGCC 3 cut(s) 929, 1321, 1371
ApoI RAATTY 1 cut(s) 891
Asp700I GAANNNNTTC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 193, 267
AspS9I GGNCC 2 cut(s) 656, 1322
AsuC2I CCSGG 2 cut(s) 195, 262
AsuHPI GGTGA 6 cut(s) 238, 994, 1437, 1458, 1479, 1500
AvaI CYCGRG 4 cut(s) 111, 616, 1283, 1324
AvaII GGWCC 1 cut(s) 656
AxyI CCTNAGG 1 cut(s) 1218
BanI GGYRCC 1 cut(s) 1222
BarI GAAGNNNNNNTAC 2 cut(s) 987, 1019
BauI CACGAG 1 cut(s) 1481
BbsI GAAGAC 1 cut(s) 1193
Bbv12I GWGCWC 1 cut(s) 782
BbvCI CCTCAGC 1 cut(s) 1264
BccI CCATC 6 cut(s) 381, 581, 737, 834, 1034, 1423
BceAI ACGGC 1 cut(s) 269
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BcnI CCSGG 2 cut(s) 195, 262
BcoDI GTCTC 6 cut(s) 176, 694, 1407, 1442, 1463, 1484
BfaI CTAG 3 cut(s) 834, 875, 1109
BfmI CTRYAG 1 cut(s) 1420
BfuAI ACCTGC 1 cut(s) 258
BisI GCNGC 4 cut(s) 36, 199, 222, 864
BlsI GCNGC 4 cut(s) 37, 200, 223, 865
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 195, 262
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 4 cut(s) 111, 616, 1283, 1324
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 656, 1322
BmiI GGNNCC 5 cut(s) 82, 150, 392, 1224, 1430
BmrFI CCNGG 2 cut(s) 195, 262
BmsI GCATC 6 cut(s) 310, 538, 651, 1297, 1472, 1514
BpiI GAAGAC 1 cut(s) 1193
BpmI CTGGAG 4 cut(s) 867, 1119, 1121, 1262
Bpu10I CCTNAGC 1 cut(s) 1264
BpuEI CTTGAG 3 cut(s) 645, 953, 1286
BpuMI CCSGG 2 cut(s) 195, 262
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 2 cut(s) 1463, 1484
BsaJI CCNNGG 5 cut(s) 76, 222, 491, 1374, 1527
BsaWI WCCGGW 2 cut(s) 1159, 1193
BsaXI ACNNNNNCTCC 2 cut(s) 1117, 1147
Bsc4I CCNNNNNNNGG 7 cut(s) 267, 870, 1331, 1425, 1454, 1475, 1496
Bse118I RCCGGY 1 cut(s) 927
Bse1I ACTGG 4 cut(s) 308, 575, 1104, 1245
Bse21I CCTNAGG 1 cut(s) 1218
BseAI TCCGGA 1 cut(s) 1193
BseDI CCNNGG 5 cut(s) 76, 222, 491, 1374, 1527
BseGI GGATG 4 cut(s) 583, 748, 1415, 1537
BseLI CCNNNNNNNGG 7 cut(s) 267, 870, 1331, 1425, 1454, 1475, 1496
BseMII CTCAG 5 cut(s) 29, 35, 1209, 1278, 1352
BseNI ACTGG 4 cut(s) 308, 575, 1104, 1245
BseRI GAGGAG 3 cut(s) 545, 864, 1083
BseYI CCCAGC 2 cut(s) 31, 751
Bsh1236I CGCG 3 cut(s) 87, 191, 224
BshFI GGCC 3 cut(s) 931, 1323, 1373
BshNI GGYRCC 1 cut(s) 1222
BsiHKAI GWGCWC 1 cut(s) 782
BsiHKCI CYCGRG 4 cut(s) 111, 616, 1283, 1324
BsiSI CCGG 5 cut(s) 194, 262, 928, 1160, 1194
BslFI GGGAC 1 cut(s) 1072
BslI CCNNNNNNNGG 7 cut(s) 267, 870, 1331, 1425, 1454, 1475, 1496
BsmAI GTCTC 6 cut(s) 176, 694, 1407, 1442, 1463, 1484
BsmBI CGTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 1072
BsmI GAATGC 3 cut(s) 412, 617, 675
BsnI GGCC 3 cut(s) 931, 1323, 1373
Bso31I GGTCTC 2 cut(s) 1463, 1484
BsoBI CYCGRG 4 cut(s) 111, 616, 1283, 1324
Bsp1286I GDGCHC 1 cut(s) 782
Bsp13I TCCGGA 1 cut(s) 1193
Bsp143I GATC 5 cut(s) 358, 487, 620, 746, 1248
BspANI GGCC 3 cut(s) 931, 1323, 1373
BspCNI CTCAG 5 cut(s) 28, 34, 1210, 1277, 1353
BspEI TCCGGA 1 cut(s) 1193
BspFNI CGCG 3 cut(s) 87, 191, 224
BspLI GGNNCC 5 cut(s) 82, 150, 392, 1224, 1430
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 3 cut(s) 482, 754, 1256
BspQI GCTCTTC 1 cut(s) 717
BspT107I GGYRCC 1 cut(s) 1222
BspTNI GGTCTC 2 cut(s) 1463, 1484
BsrFI RCCGGY 1 cut(s) 927
BsrI ACTGG 4 cut(s) 308, 575, 1104, 1245
BssAI RCCGGY 1 cut(s) 927
BssECI CCNNGG 5 cut(s) 76, 222, 491, 1374, 1527
BssMI GATC 5 cut(s) 358, 487, 620, 746, 1248
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 1481
BssT1I CCWWGG 2 cut(s) 491, 1374
Bst2BI CACGAG 1 cut(s) 1481
Bst4CI ACNGT 2 cut(s) 395, 1544
Bst6I CTCTTC 2 cut(s) 291, 717
BstACI GRCGYC 1 cut(s) 441
BstC8I GCNNGC 7 cut(s) 147, 312, 515, 802, 920, 1321, 1371
BstDEI CTNAG 9 cut(s) 15, 21, 414, 759, 786, 1218, 1227, 1264, 1361
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 4 cut(s) 583, 748, 1415, 1537
BstFNI CGCG 3 cut(s) 87, 191, 224
BstHHI GCGC 2 cut(s) 193, 267
BstKTI GATC 5 cut(s) 361, 490, 623, 749, 1251
BstMAI GTCTC 6 cut(s) 176, 694, 1407, 1442, 1463, 1484
BstMBI GATC 5 cut(s) 358, 487, 620, 746, 1248
BstMWI GCNNNNNNNGC 7 cut(s) 264, 281, 320, 851, 860, 928, 1366
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 193, 260
BstSFI CTRYAG 1 cut(s) 1420
BstUI CGCG 3 cut(s) 87, 191, 224
BstV2I GAAGAC 1 cut(s) 1193
Bsu36I CCTNAGG 1 cut(s) 1218
BsuRI GGCC 3 cut(s) 931, 1323, 1373
BtgI CCRYGG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 4 cut(s) 583, 748, 1415, 1537
BveI ACCTGC 1 cut(s) 258
Cac8I GCNNGC 7 cut(s) 147, 312, 515, 802, 920, 1321, 1371
CaiI CAGNNNCTG 2 cut(s) 551, 1319
CfoI GCGC 2 cut(s) 193, 267
Cfr10I RCCGGY 1 cut(s) 927
Cfr13I GGNCC 2 cut(s) 656, 1322
Cfr42I CCGCGG 1 cut(s) 225
CseI GACGC 2 cut(s) 197, 449
Csp6I GTAC 3 cut(s) 301, 342, 682
CviAII CATG 1 cut(s) 518
CviQI GTAC 3 cut(s) 301, 342, 682
DdeI CTNAG 9 cut(s) 15, 21, 414, 759, 786, 1218, 1227, 1264, 1361
DpnI GATC 5 cut(s) 360, 489, 622, 748, 1250
DpnII GATC 5 cut(s) 358, 487, 620, 746, 1248
DraI TTTAAA 1 cut(s) 1069
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 929
Eam1104I CTCTTC 2 cut(s) 291, 717
EarI CTCTTC 2 cut(s) 291, 717
EciI GGCGGA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 491, 1374
Eco147I AGGCCT 1 cut(s) 1373
Eco31I GGTCTC 2 cut(s) 1463, 1484
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 2 cut(s) 917, 1187
Eco81I CCTNAGG 1 cut(s) 1218
Eco88I CYCGRG 4 cut(s) 111, 616, 1283, 1324
EcoRI GAATTC 1 cut(s) 891
EcoT14I CCWWGG 2 cut(s) 491, 1374
EcoT22I ATGCAT 2 cut(s) 644, 675
ErhI CCWWGG 2 cut(s) 491, 1374
Esp3I CGTCTC 1 cut(s) 176
FaeI CATG 1 cut(s) 521
FalI AAGNNNNNCTT 4 cut(s) 687, 719, 714, 746
FaqI GGGAC 1 cut(s) 1072
FatI CATG 1 cut(s) 517
FauI CCCGC 3 cut(s) 92, 911, 1049
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 4 cut(s) 36, 199, 222, 864
FokI GGATG 4 cut(s) 590, 755, 1402, 1544
Fsp4HI GCNGC 4 cut(s) 36, 199, 222, 864
FspBI CTAG 3 cut(s) 834, 875, 1109
GlaI GCGC 2 cut(s) 192, 266
GluI GCNGC 4 cut(s) 36, 199, 222, 864
GsaI CCCAGC 2 cut(s) 35, 755
GsuI CTGGAG 4 cut(s) 867, 1119, 1121, 1262
HaeIII GGCC 3 cut(s) 931, 1323, 1373
HapII CCGG 5 cut(s) 194, 262, 928, 1160, 1194
HgaI GACGC 2 cut(s) 197, 449
HhaI GCGC 2 cut(s) 193, 267
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 1 cut(s) 521
Hin6I GCGC 2 cut(s) 191, 265
HinP1I GCGC 2 cut(s) 191, 265
HincII GTYRAC 4 cut(s) 118, 216, 1311, 1387
HindII GTYRAC 4 cut(s) 118, 216, 1311, 1387
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 6 cut(s) 212, 278, 371, 1022, 1105, 1207
HpaI GTTAAC 1 cut(s) 1387
HpaII CCGG 5 cut(s) 194, 262, 928, 1160, 1194
HphI GGTGA 6 cut(s) 238, 994, 1437, 1458, 1479, 1500
Hpy166II GTNNAC 6 cut(s) 118, 216, 985, 1311, 1387, 1524
Hpy188III TCNNGA 4 cut(s) 662, 731, 1136, 1194
Hpy8I GTNNAC 6 cut(s) 118, 216, 985, 1311, 1387, 1524
Hpy99I CGWCG 4 cut(s) 143, 290, 293, 296
HpyAV CCTTC 7 cut(s) 49, 60, 166, 223, 232, 689, 864
HpyCH4III ACNGT 2 cut(s) 395, 1544
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 4 cut(s) 517, 642, 673, 911
HpyF10VI GCNNNNNNNGC 7 cut(s) 264, 281, 320, 851, 860, 928, 1366
HpyF3I CTNAG 9 cut(s) 15, 21, 414, 759, 786, 1218, 1227, 1264, 1361
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 1 cut(s) 521
HspAI GCGC 2 cut(s) 191, 265
Kpn2I TCCGGA 1 cut(s) 1193
KspAI GTTAAC 1 cut(s) 1387
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 5 cut(s) 358, 487, 620, 746, 1248
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 3 cut(s) 154, 851, 1316
LweI GCATC 6 cut(s) 310, 538, 651, 1297, 1472, 1514
MaeI CTAG 3 cut(s) 834, 875, 1109
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 251, 1016, 1485
MalI GATC 5 cut(s) 360, 489, 622, 748, 1250
MboI GATC 5 cut(s) 358, 487, 620, 746, 1248
MboII GAAGA 3 cut(s) 308, 734, 1193
MhlI GDGCHC 1 cut(s) 782
MluCI AATT 5 cut(s) 366, 455, 891, 903, 1329
MlyI GAGTC 3 cut(s) 221, 272, 1216
MmeI TCCRAC 4 cut(s) 135, 162, 189, 555
Mph1103I ATGCAT 2 cut(s) 644, 675
MroI TCCGGA 1 cut(s) 1193
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 4 cut(s) 381, 999, 1068, 1386
MslI CAYNNNNRTG 2 cut(s) 501, 1303
MspA1I CMGCKG 2 cut(s) 224, 866
MspI CCGG 5 cut(s) 194, 262, 928, 1160, 1194
MspR9I CCNGG 2 cut(s) 195, 262
Mva1269I GAATGC 3 cut(s) 412, 617, 675
MvnI CGCG 3 cut(s) 87, 191, 224
MwoI GCNNNNNNNGC 7 cut(s) 264, 281, 320, 851, 860, 928, 1366
NciI CCSGG 2 cut(s) 195, 262
NdeII GATC 5 cut(s) 358, 487, 620, 746, 1248
NlaIII CATG 1 cut(s) 521
NlaIV GGNNCC 5 cut(s) 82, 150, 392, 1224, 1430
NmuCI GTSAC 2 cut(s) 1016, 1485
NsiI ATGCAT 2 cut(s) 644, 675
NspI RCATGY 1 cut(s) 521
OliI CACNNNNGTG 1 cut(s) 1303
PceI AGGCCT 1 cut(s) 1373
PciSI GCTCTTC 1 cut(s) 717
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 391
PctI GAATGC 3 cut(s) 412, 617, 675
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 371, 1022, 1105
PkrI GCNGC 4 cut(s) 37, 200, 223, 865
PleI GAGTC 3 cut(s) 220, 272, 1215
PpsI GAGTC 3 cut(s) 220, 272, 1215
PspFI CCCAGC 2 cut(s) 31, 751
PspN4I GGNNCC 5 cut(s) 82, 150, 392, 1224, 1430
PspPI GGNCC 2 cut(s) 656, 1322
PstNI CAGNNNCTG 2 cut(s) 551, 1319
RsaI GTAC 3 cut(s) 302, 343, 683
RsaNI GTAC 3 cut(s) 301, 342, 682
RseI CAYNNNNRTG 2 cut(s) 501, 1303
SacII CCGCGG 1 cut(s) 225
SalI GTCGAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 4 cut(s) 381, 999, 1068, 1386
SatI GCNGC 4 cut(s) 36, 199, 222, 864
Sau3AI GATC 5 cut(s) 358, 487, 620, 746, 1248
Sau96I GGNCC 2 cut(s) 656, 1322
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 3 cut(s) 221, 272, 1216
ScrFI CCNGG 2 cut(s) 195, 262
SduI GDGCHC 1 cut(s) 782
SfaNI GCATC 6 cut(s) 310, 538, 651, 1297, 1472, 1514
SfcI CTRYAG 1 cut(s) 1420
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 2 cut(s) 501, 1303
SmlI CTYRAG 3 cut(s) 660, 968, 1301
SmoI CTYRAG 3 cut(s) 660, 968, 1301
Sse9I AATT 5 cut(s) 366, 455, 891, 903, 1329
SseBI AGGCCT 1 cut(s) 1373
SspMI CTAG 3 cut(s) 834, 875, 1109
StuI AGGCCT 1 cut(s) 1373
StyD4I CCNGG 2 cut(s) 193, 260
StyI CCWWGG 2 cut(s) 491, 1374
TaaI ACNGT 2 cut(s) 395, 1544
TaiI ACGT 1 cut(s) 144
TaqI TCGA 5 cut(s) 42, 215, 294, 623, 631
TasI AATT 5 cut(s) 366, 455, 891, 903, 1329
TatI WGTACW 2 cut(s) 300, 681
TauI GCSGC 4 cut(s) 38, 201, 224, 866
TfiI GAWTC 3 cut(s) 371, 1022, 1105
Tru1I TTAA 4 cut(s) 381, 999, 1068, 1386
Tru9I TTAA 4 cut(s) 381, 999, 1068, 1386
TseFI GTSAC 2 cut(s) 1016, 1485
Tsp45I GTSAC 2 cut(s) 1016, 1485
TspDTI ATGAA 1 cut(s) 1008
TspGWI ACGGA 2 cut(s) 141, 200
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 891
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 3 cut(s) 834, 875, 1109
ZrmI AGTACT 1 cut(s) 302
Zsp2I ATGCAT 2 cut(s) 644, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.