Prupe.2G083500_v2.0.a1

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010508
Physical Location & Seq
Reverse (-)
13266923 .. 13270648
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G083500.2

Sequence Viewer

Length: 1344 bp
ATGGCTGAGCTATCTCTGGATTGCTATGATGGTGCATCGCCGGTGATGGTTCCTCAGCATACAGCTCATGGCCATGAATCAACCGCTGCAGTGAAGTTGCAGAAAGTGTACAGGAGTTATAGAACCCGGCGTAGGCTAGCAGACTCGGCTGTGGTTGCTGAGGAGCTATGGTGGCAAGCTATAGATTATGCAAGGTTGAATCATAGTACGATTTCGTTCTTCGATTATCATAAATCTGAAACCGCAGCCTCACGATGGAGTAGGATCAGCTTATTTGCATCCAAGCTGGGGAAGGGATTATCAAAGGATGCTAAGGCACAAAAGTTAGCTTTCCAACATTGGATCGAAGCGATTGATCCAAGACATCGTTATGGCCATAATCTGAACTATTACTATGATGAGTGGTGTAGAGGTGATGCAGGACAGCCCTTTTTCTACTGGTTGGACGTTGGAGATGGCAAGGAAGTTGATCTCAAGGATTGTCCAAGATCAAAGCTGCAACAAGAATGCATCAAGTATCTTGGACCGCAAGAGAGGGAACACTTCGAATATATCATTTTTGAAGGGACCTTGGTGCACAGAGGAACAGGATATCTTCTTGATACAAATCAAGGAATGGAAGATTCCAAATGGATTTTCGTGATGAGCACATCGAAGAAACTTTATGCTGGCGAGAAGAAGAAGGGATTATTTCATCATTCCAGCTTCCTTGCCGGAGGGGCCACCTTGGCTGCTGGAAGACTTAGAGCAGAACATGGAAAGCTCAAGTCTGTATCGGCATACAGCGGACATTACCGTCCAACCAATGAGAACCTTGGCAGCTTCCTCATCTTCCTTAAGGAAAGTGGAGTTGCCCTTGATGGAGTTCAGGTACTTTCTCCCATGGAAGACAATGAGAGCTATGGTTTGAGCAAATCAATTCAACCTGGAAGAACATATGGACAAACAAAAGCTGAAAACCCTGAGCTTCATGCTTCCAGGGTGGAAGAAAGCAATCAACAGTCTGAATCATGTACATTTTCAGAAACAAAAAGAATAAGTAGTTACCAAAGAACCATATCCAGAAATCTCTTCAACCTCCGAACAAGTGTCCCTAAGAAAGAATTATTGCAAAGGATCAACTCCAAGAAGGAAGCAAGTTCATGTCAACTAGGAGACCAACTGTCTTTGAAGTGGTCAACAGGAGCAGGCCCTAGGATTGGGTCTGTTGCAGACTACCCTCTGAAGTTAAGGCTACAAGCACTAGAATTTGTAAGCCTCTCTCCTAGGGTCTCCCTCGCGGCATCCCCATCGGAACCTCCATCAGGTCTCACATCATTTACAACTTCTTGCAGGATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

448

Amino Acids

50.29

Weight (kDa)

9.0

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 795
AccII CGCG 1 cut(s) 1280
AciI CCGC 5 cut(s) 84, 243, 527, 786, 1280
AclWI GGATC 4 cut(s) 272, 350, 350, 1124
AcoI YGGCCR 2 cut(s) 70, 373
AcsI RAATTY 1 cut(s) 1247
AcuI CTGAAG 1 cut(s) 1244
AfaI GTAC 4 cut(s) 110, 208, 873, 1015
AfiI CCNNNNNNNGG 5 cut(s) 132, 255, 288, 1199, 1304
AflII CTTAAG 1 cut(s) 836
AgsI TTSAA 5 cut(s) 199, 563, 923, 1075, 1171
AhdI GACNNNNNGTC 1 cut(s) 1162
AjnI CCWGG 2 cut(s) 925, 977
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
Alw21I GWGCWC 2 cut(s) 579, 650
Alw26I GTCTC 3 cut(s) 1149, 1276, 1313
Alw44I GTGCAC 1 cut(s) 575
AlwI GGATC 4 cut(s) 272, 350, 350, 1124
AoxI GGCC 4 cut(s) 70, 373, 720, 1189
ApaLI GTGCAC 1 cut(s) 575
ApeKI GCWGC 5 cut(s) 86, 245, 496, 731, 819
ApoI RAATTY 1 cut(s) 1247
AspA2I CCTAGG 2 cut(s) 1193, 1265
AspS9I GGNCC 4 cut(s) 524, 567, 720, 1190
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 2 cut(s) 55, 425
AsuII TTCGAA 1 cut(s) 546
AsuNHI GCTAGC 1 cut(s) 136
AvaII GGWCC 2 cut(s) 524, 567
AvrII CCTAGG 2 cut(s) 1193, 1265
BaeGI GKGCMC 1 cut(s) 579
BalI TGGCCA 2 cut(s) 72, 375
BbsI GAAGAC 2 cut(s) 745, 894
Bbv12I GWGCWC 2 cut(s) 579, 650
BbvCI CCTCAGC 2 cut(s) 54, 159
BbvI GCAGC 5 cut(s) 73, 257, 483, 718, 831
BccI CCATC 7 cut(s) 23, 40, 249, 449, 854, 1297, 1309
BcgI CGANNNNNNTGC 2 cut(s) 1272, 1306
BciT130I CCWGG 2 cut(s) 927, 979
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 3 cut(s) 1149, 1276, 1313
BfaI CTAG 5 cut(s) 137, 1151, 1194, 1244, 1266
BfmI CTRYAG 2 cut(s) 87, 180
BfrI CTTAAG 1 cut(s) 836
BglI GCCNNNNNGGC 2 cut(s) 719, 728
BisI GCNGC 6 cut(s) 87, 246, 497, 732, 820, 1281
BlnI CCTAGG 2 cut(s) 1193, 1265
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 6 cut(s) 88, 247, 498, 733, 821, 1282
Bme1390I CCNGG 3 cut(s) 127, 927, 979
Bme18I GGWCC 2 cut(s) 524, 567
BmeRI GACNNNNNGTC 1 cut(s) 1162
BmgT120I GGNCC 4 cut(s) 524, 567, 720, 1190
BmiI GGNNCC 4 cut(s) 51, 568, 721, 1296
BmrFI CCNGG 3 cut(s) 127, 927, 979
BmsI GCATC 6 cut(s) 44, 287, 298, 406, 519, 1292
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 745, 894
Bpu10I CCTNAGC 4 cut(s) 54, 159, 312, 963
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 546
BpuEI CTTGAG 2 cut(s) 458, 749
BpuMI CCSGG 1 cut(s) 127
BsaBI GATNNNNATC 1 cut(s) 606
BsaI GGTCTC 3 cut(s) 1149, 1276, 1313
BsaJI CCNNGG 7 cut(s) 570, 726, 814, 882, 978, 1193, 1265
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 855, 885
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 255, 288, 1199, 1304
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 443
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 2 cut(s) 927, 979
BseDI CCNNGG 7 cut(s) 570, 726, 814, 882, 978, 1193, 1265
BseGI GGATG 3 cut(s) 278, 313, 1283
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 5 cut(s) 132, 255, 288, 1199, 1304
BseMII CTCAG 3 cut(s) 68, 150, 954
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 1 cut(s) 176
BseSI GKGCMC 1 cut(s) 579
BseXI GCAGC 5 cut(s) 73, 257, 483, 718, 831
BseYI CCCAGC 1 cut(s) 286
Bsh1236I CGCG 1 cut(s) 1280
BshFI GGCC 4 cut(s) 72, 375, 722, 1191
BsiHKAI GWGCWC 2 cut(s) 579, 650
BsiSI CCGG 3 cut(s) 41, 127, 714
BslFI GGGAC 2 cut(s) 580, 1076
BslI CCNNNNNNNGG 5 cut(s) 132, 255, 288, 1199, 1304
BsmAI GTCTC 3 cut(s) 1149, 1276, 1313
BsmFI GGGAC 2 cut(s) 580, 1076
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 4 cut(s) 72, 375, 722, 1191
Bso31I GGTCTC 3 cut(s) 1149, 1276, 1313
Bsp119I TTCGAA 1 cut(s) 546
Bsp1286I GDGCHC 2 cut(s) 579, 650
Bsp1407I TGTACA 2 cut(s) 108, 1013
Bsp143I GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 5 cut(s) 84, 243, 527, 786, 1280
BspANI GGCC 4 cut(s) 72, 375, 722, 1191
BspCNI CTCAG 3 cut(s) 67, 151, 955
BspFNI CGCG 1 cut(s) 1280
BspLI GGNNCC 4 cut(s) 51, 568, 721, 1296
BspMAI CTGCAG 1 cut(s) 91
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 4 cut(s) 272, 350, 350, 1124
BspT104I TTCGAA 1 cut(s) 546
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 3 cut(s) 1149, 1276, 1313
BsrFI RCCGGY 1 cut(s) 40
BsrGI TGTACA 2 cut(s) 108, 1013
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 7 cut(s) 570, 726, 814, 882, 978, 1193, 1265
BssMI GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
BssT1I CCWWGG 6 cut(s) 570, 726, 814, 882, 1193, 1265
Bst2UI CCWGG 2 cut(s) 927, 979
Bst4CI ACNGT 3 cut(s) 797, 1002, 1164
Bst6I CTCTTC 1 cut(s) 1076
BstAFI CTTAAG 1 cut(s) 836
BstAUI TGTACA 2 cut(s) 108, 1013
BstBI TTCGAA 1 cut(s) 546
BstC8I GCNNGC 4 cut(s) 138, 177, 670, 1189
BstDEI CTNAG 7 cut(s) 6, 54, 159, 312, 743, 963, 1095
BstDSI CCRYGG 1 cut(s) 882
BstENI CCTNNNNNAGG 1 cut(s) 1302
BstF5I GGATG 3 cut(s) 278, 313, 1283
BstFNI CGCG 1 cut(s) 1280
BstKTI GATC 6 cut(s) 267, 345, 358, 472, 491, 1119
BstMAI GTCTC 3 cut(s) 1149, 1276, 1313
BstMBI GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 172, 719, 728
BstNI CCWGG 2 cut(s) 927, 979
BstSCI CCNGG 3 cut(s) 125, 925, 977
BstSFI CTRYAG 2 cut(s) 87, 180
BstSLI GKGCMC 1 cut(s) 579
BstUI CGCG 1 cut(s) 1280
BstV1I GCAGC 5 cut(s) 73, 257, 483, 718, 831
BstV2I GAAGAC 2 cut(s) 745, 894
BsuRI GGCC 4 cut(s) 72, 375, 722, 1191
BtgI CCRYGG 1 cut(s) 882
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 3 cut(s) 278, 313, 1283
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 138, 177, 670, 1189
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 4 cut(s) 524, 567, 720, 1190
Csp6I GTAC 4 cut(s) 109, 207, 872, 1014
CviAII CATG 7 cut(s) 68, 74, 755, 883, 971, 1011, 1143
CviQI GTAC 4 cut(s) 109, 207, 872, 1014
DdeI CTNAG 7 cut(s) 6, 54, 159, 312, 743, 963, 1095
DpnI GATC 6 cut(s) 266, 344, 357, 471, 490, 1118
DpnII GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
DrdI GACNNNNNNGTC 1 cut(s) 795
DriI GACNNNNNGTC 1 cut(s) 1162
DseDI GACNNNNNNGTC 1 cut(s) 795
EaeI YGGCCR 2 cut(s) 70, 373
Eam1104I CTCTTC 1 cut(s) 1076
Eam1105I GACNNNNNGTC 1 cut(s) 1162
EarI CTCTTC 1 cut(s) 1076
Eco130I CCWWGG 6 cut(s) 570, 726, 814, 882, 1193, 1265
Eco31I GGTCTC 3 cut(s) 1149, 1276, 1313
Eco32I GATATC 1 cut(s) 593
Eco47I GGWCC 2 cut(s) 524, 567
Eco57I CTGAAG 1 cut(s) 1244
EcoNI CCTNNNNNAGG 1 cut(s) 1302
EcoO109I RGGNCCY 2 cut(s) 567, 1190
EcoRII CCWGG 2 cut(s) 925, 977
EcoRV GATATC 1 cut(s) 593
EcoT14I CCWWGG 6 cut(s) 570, 726, 814, 882, 1193, 1265
EcoT22I ATGCAT 1 cut(s) 512
ErhI CCWWGG 6 cut(s) 570, 726, 814, 882, 1193, 1265
FaeI CATG 7 cut(s) 71, 77, 758, 886, 974, 1014, 1146
FaqI GGGAC 2 cut(s) 580, 1076
FatI CATG 7 cut(s) 67, 73, 754, 882, 970, 1010, 1142
FauNDI CATATG 1 cut(s) 937
Fnu4HI GCNGC 6 cut(s) 87, 246, 497, 732, 820, 1281
FokI GGATG 3 cut(s) 265, 320, 1270
Fsp4HI GCNGC 6 cut(s) 87, 246, 497, 732, 820, 1281
FspBI CTAG 5 cut(s) 137, 1151, 1194, 1244, 1266
GluI GCNGC 6 cut(s) 87, 246, 497, 732, 820, 1281
GsaI CCCAGC 1 cut(s) 290
HaeIII GGCC 4 cut(s) 72, 375, 722, 1191
HapII CCGG 3 cut(s) 41, 127, 714
Hin1II CATG 7 cut(s) 71, 77, 758, 886, 974, 1014, 1146
HincII GTYRAC 2 cut(s) 1148, 1179
HindII GTYRAC 2 cut(s) 1148, 1179
HinfI GANTC 5 cut(s) 77, 143, 199, 623, 1007
HpaII CCGG 3 cut(s) 41, 127, 714
HphI GGTGA 2 cut(s) 55, 425
Hpy166II GTNNAC 4 cut(s) 109, 577, 1148, 1179
Hpy188I TCNGA 7 cut(s) 238, 384, 1006, 1024, 1082, 1224, 1294
Hpy188III TCNNGA 5 cut(s) 17, 252, 599, 640, 1062
Hpy8I GTNNAC 4 cut(s) 109, 577, 1148, 1179
HpyAV CCTTC 4 cut(s) 286, 557, 676, 1123
HpyCH4III ACNGT 3 cut(s) 797, 1002, 1164
HpyCH4IV ACGT 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 172, 719, 728
HpyF3I CTNAG 7 cut(s) 6, 54, 159, 312, 743, 963, 1095
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 7 cut(s) 71, 77, 758, 886, 974, 1014, 1146
Kzo9I GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
LmnI GCTCC 2 cut(s) 163, 1184
Lsp1109I GCAGC 5 cut(s) 73, 257, 483, 718, 831
LweI GCATC 6 cut(s) 44, 287, 298, 406, 519, 1292
MaeI CTAG 5 cut(s) 137, 1151, 1194, 1244, 1266
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 1 cut(s) 1043
MalI GATC 6 cut(s) 266, 344, 357, 471, 490, 1118
MboI GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
MhlI GDGCHC 2 cut(s) 579, 650
MlsI TGGCCA 2 cut(s) 72, 375
MluCI AATT 3 cut(s) 918, 1103, 1247
MluNI TGGCCA 2 cut(s) 72, 375
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 4 cut(s) 358, 423, 430, 824
Mox20I TGGCCA 2 cut(s) 72, 375
Mph1103I ATGCAT 1 cut(s) 512
MscI TGGCCA 2 cut(s) 72, 375
MseI TTAA 2 cut(s) 837, 1229
MslI CAYNNNNRTG 2 cut(s) 72, 369
Msp20I TGGCCA 2 cut(s) 72, 375
MspA1I CMGCKG 2 cut(s) 86, 786
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 3 cut(s) 41, 127, 714
MspR9I CCNGG 3 cut(s) 127, 927, 979
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 2 cut(s) 927, 979
MvnI CGCG 1 cut(s) 1280
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 172, 719, 728
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 1 cut(s) 882
NdeI CATATG 1 cut(s) 937
NdeII GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
NheI GCTAGC 1 cut(s) 136
NlaIII CATG 7 cut(s) 71, 77, 758, 886, 974, 1014, 1146
NlaIV GGNNCC 4 cut(s) 51, 568, 721, 1296
NmeAIII GCCGAG 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 512
NspV TTCGAA 1 cut(s) 546
PctI GAATGC 1 cut(s) 512
PfeI GAWTC 4 cut(s) 77, 199, 623, 1007
PkrI GCNGC 6 cut(s) 88, 247, 498, 733, 821, 1282
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 567
Psp5II RGGWCCY 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 925, 977
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 925, 977
PspN4I GGNNCC 4 cut(s) 51, 568, 721, 1296
PspPI GGNCC 4 cut(s) 524, 567, 720, 1190
PspPPI RGGWCCY 1 cut(s) 567
PstI CTGCAG 1 cut(s) 91
RsaI GTAC 4 cut(s) 110, 208, 873, 1015
RsaNI GTAC 4 cut(s) 109, 207, 872, 1014
RseI CAYNNNNRTG 2 cut(s) 72, 369
SaqAI TTAA 2 cut(s) 837, 1229
SatI GCNGC 6 cut(s) 87, 246, 497, 732, 820, 1281
Sau3AI GATC 6 cut(s) 264, 342, 355, 469, 488, 1116
Sau96I GGNCC 4 cut(s) 524, 567, 720, 1190
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 127, 927, 979
SduI GDGCHC 2 cut(s) 579, 650
SfaNI GCATC 6 cut(s) 44, 287, 298, 406, 519, 1292
SfcI CTRYAG 2 cut(s) 87, 180
SfuI TTCGAA 1 cut(s) 546
SgrAI CRCCGGYG 1 cut(s) 40
SinI GGWCC 2 cut(s) 524, 567
SmiMI CAYNNNNRTG 2 cut(s) 72, 369
SmlI CTYRAG 3 cut(s) 473, 764, 836
SmoI CTYRAG 3 cut(s) 473, 764, 836
Sse9I AATT 3 cut(s) 918, 1103, 1247
SsiI CCGC 5 cut(s) 84, 243, 527, 786, 1280
SspMI CTAG 5 cut(s) 137, 1151, 1194, 1244, 1266
StyD4I CCNGG 3 cut(s) 125, 925, 977
StyI CCWWGG 6 cut(s) 570, 726, 814, 882, 1193, 1265
TaaI ACNGT 3 cut(s) 797, 1002, 1164
TaiI ACGT 1 cut(s) 450
TaqI TCGA 4 cut(s) 222, 345, 546, 653
TasI AATT 3 cut(s) 918, 1103, 1247
TatI WGTACW 2 cut(s) 108, 1013
TauI GCSGC 1 cut(s) 1283
TfiI GAWTC 4 cut(s) 77, 199, 623, 1007
Tru1I TTAA 2 cut(s) 837, 1229
Tru9I TTAA 2 cut(s) 837, 1229
TscAI CASTG 1 cut(s) 96
TseI GCWGC 5 cut(s) 86, 245, 496, 731, 819
TspDTI ATGAA 4 cut(s) 90, 683, 959, 1131
TspRI CASTG 1 cut(s) 96
Vha464I CTTAAG 1 cut(s) 836
VneI GTGCAC 1 cut(s) 575
VpaK11BI GGWCC 2 cut(s) 524, 567
XagI CCTNNNNNAGG 1 cut(s) 1302
XapI RAATTY 1 cut(s) 1247
XmaJI CCTAGG 2 cut(s) 1193, 1265
XspI CTAG 5 cut(s) 137, 1151, 1194, 1244, 1266
Zsp2I ATGCAT 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.