Rw3G016320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0010508
Physical Location & Seq
Reverse (-)
17205592 .. 17208840
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016320.1

Sequence Viewer

Length: 777 bp
ATGGAGGTTCAGGCCCAGAGCCTCTCGGGTTTCGACCATCTCCAGTCGAAGCCATTCTCCTACTCCCCCAACGGCGTCCTCGATTCCCGCGACTTTCGGAGCTCCGAATTGTCCCCGAAATGCGAGGCCGCGAGTCCCAAGGCGAGTGAGGCGGACGCGTCGGCGGTGAAGCTGCAGAAGGTGTACAGGAGTTACCGGACCCGGCGCAGGTTGGCTGACTCCGCCGTCGTGGCTGAAGAGCTCTGGTGGCAAGCGATAGACTATGCGAGACTGAACCACAGTACCATTTCTTTCTTTAATTTCTTGAAGCCCGAATCTGCGGCTTCACGCTGGAAACGTTATAGCTTGAATGCTTCTAAGGTGGGCAAGGGATTGTCTAAGGACGCCAAAGCTCAGAAACTGGCTTTTCAGCACTGGATTGAAGCTATTGATCCAAGGCACAGGTATGGGCATAGCTTGCATGTGTATTATGAAGAATGGTGTAAAGCAGATGCTGGCCAGCCATTTTTTTACTGGTTGGATATAGGAGATGGCAAAGAGCTTGATCTCAAGAAGTGCCCAAGATCAGAGCTTCGACAGCAATGCATTAGATATCTTGGACCTCACGAGAGAATACATTACGAGTATGTTGTTGCTGATGGGCAAATTATTCACTCACAAACTGGAGAGCTACTTGATACAAATGAGGGATCGAAAGCTAAGTGGATCTTTGTTATGAGCACCACTAAGAAACTCTATGCTGGTGAAAAAAAGAAAGGAGTTTTTCCATCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

258

Amino Acids

29.61

Weight (kDa)

9.17

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 198
AccII CGCG 3 cut(s) 90, 131, 158
AciI CCGC 6 cut(s) 88, 129, 152, 164, 222, 320
AclI AACGTT 1 cut(s) 337
AclWI GGATC 3 cut(s) 425, 697, 713
AcoI YGGCCR 1 cut(s) 496
AcuI CTGAAG 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 75, 384
AfaI GTAC 2 cut(s) 185, 283
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 307, 349, 422
Alw21I GWGCWC 3 cut(s) 104, 243, 722
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 3 cut(s) 425, 697, 713
AlwNI CAGNNNCTG 2 cut(s) 400, 494
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 3 cut(s) 12, 126, 496
ApeKI GCWGC 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 3 cut(s) 13, 198, 599
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 178, 755
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 2 cut(s) 198, 599
BaeGI GKGCMC 1 cut(s) 560
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BalI TGGCCA 1 cut(s) 498
BanII GRGCYC 2 cut(s) 104, 243
BauI CACGAG 1 cut(s) 605
Bbv12I GWGCWC 3 cut(s) 104, 243, 722
BbvI GCAGC 1 cut(s) 159
BccI CCATC 4 cut(s) 45, 524, 632, 775
BceAI ACGGC 2 cut(s) 88, 209
BcgI CGANNNNNNTGC 2 cut(s) 564, 598
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 1 cut(s) 775
BfmI CTRYAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 3 cut(s) 129, 173, 321
BlsI GCNGC 3 cut(s) 130, 174, 322
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 2 cut(s) 198, 599
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 13, 198, 599
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 2 cut(s) 26, 684
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 2 cut(s) 75, 384
BsaJI CCNNGG 2 cut(s) 138, 434
BsaWI WCCGGW 1 cut(s) 195
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 5 cut(s) 43, 405, 419, 518, 667
Bse3DI GCAATG 1 cut(s) 587
BseDI CCNNGG 2 cut(s) 138, 434
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 5 cut(s) 43, 405, 419, 518, 667
BseSI GKGCMC 1 cut(s) 560
BseXI GCAGC 1 cut(s) 159
Bsh1236I CGCG 3 cut(s) 90, 131, 158
BshFI GGCC 3 cut(s) 14, 128, 498
BsiHKAI GWGCWC 3 cut(s) 104, 243, 722
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 196, 202
BslFI GGGAC 2 cut(s) 97, 120
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 97, 120
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 3 cut(s) 14, 128, 498
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 4 cut(s) 104, 243, 560, 722
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 5 cut(s) 430, 544, 563, 689, 705
BspACI CCGC 6 cut(s) 88, 129, 152, 164, 222, 320
BspANI GGCC 3 cut(s) 14, 128, 498
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 3 cut(s) 90, 131, 158
BspLI GGNNCC 1 cut(s) 200
BspMAI CTGCAG 1 cut(s) 177
BspMI ACCTGC 1 cut(s) 198
BspPI GGATC 3 cut(s) 425, 697, 713
BspQI GCTCTTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 587
BsrGI TGTACA 1 cut(s) 183
BsrI ACTGG 5 cut(s) 43, 405, 419, 518, 667
BssECI CCNNGG 2 cut(s) 138, 434
BssMI GATC 5 cut(s) 430, 544, 563, 689, 705
BssNI GRCGYC 2 cut(s) 75, 384
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 2 cut(s) 138, 434
Bst2BI CACGAG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 231
BstACI GRCGYC 2 cut(s) 75, 384
BstAPI GCANNNNNTGC 1 cut(s) 457
BstAUI TGTACA 1 cut(s) 183
BstC8I GCNNGC 4 cut(s) 252, 458, 496, 500
BstDEI CTNAG 5 cut(s) 357, 378, 393, 699, 726
BstFNI CGCG 3 cut(s) 90, 131, 158
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 5 cut(s) 433, 547, 566, 692, 708
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 5 cut(s) 430, 544, 563, 689, 705
BstMWI GCNNNNNNNGC 6 cut(s) 149, 221, 230, 247, 457, 577
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 173
BstSLI GKGCMC 1 cut(s) 560
BstUI CGCG 3 cut(s) 90, 131, 158
BstV1I GCAGC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 705
BstYI RGATCY 1 cut(s) 705
BsuRI GGCC 3 cut(s) 14, 128, 498
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 198
Cac8I GCNNGC 4 cut(s) 252, 458, 496, 500
CaiI CAGNNNCTG 2 cut(s) 400, 494
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 3 cut(s) 13, 198, 599
CseI GACGC 4 cut(s) 64, 147, 164, 392
Csp6I GTAC 2 cut(s) 184, 282
CviAII CATG 1 cut(s) 461
CviQI GTAC 2 cut(s) 184, 282
DdeI CTNAG 5 cut(s) 357, 378, 393, 699, 726
DpnI GATC 5 cut(s) 432, 546, 565, 691, 707
DpnII GATC 5 cut(s) 430, 544, 563, 689, 705
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 496
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 2 cut(s) 167, 211
Ecl136II GAGCTC 2 cut(s) 102, 241
Eco130I CCWWGG 2 cut(s) 138, 434
Eco24I GRGCYC 2 cut(s) 104, 243
Eco32I GATATC 1 cut(s) 593
Eco47I GGWCC 2 cut(s) 198, 599
Eco53kI GAGCTC 2 cut(s) 102, 241
Eco57I CTGAAG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 25
EcoICRI GAGCTC 2 cut(s) 102, 241
EcoRV GATATC 1 cut(s) 593
EcoT14I CCWWGG 2 cut(s) 138, 434
EcoT22I ATGCAT 1 cut(s) 587
EcoT38I GRGCYC 2 cut(s) 104, 243
ErhI CCWWGG 2 cut(s) 138, 434
FaeI CATG 1 cut(s) 464
FalI AAGNNNNNCTT 2 cut(s) 692, 724
FaqI GGGAC 2 cut(s) 97, 120
FatI CATG 1 cut(s) 460
FauI CCCGC 1 cut(s) 95
Fnu4HI GCNGC 3 cut(s) 129, 173, 321
FriOI GRGCYC 2 cut(s) 104, 243
Fsp4HI GCNGC 3 cut(s) 129, 173, 321
FspBI CTAG 1 cut(s) 775
GlaI GCGC 1 cut(s) 206
GluI GCNGC 3 cut(s) 129, 173, 321
GsuI CTGGAG 2 cut(s) 26, 684
HaeIII GGCC 3 cut(s) 14, 128, 498
HapII CCGG 2 cut(s) 196, 202
HgaI GACGC 4 cut(s) 64, 147, 164, 392
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 2 cut(s) 75, 384
Hin1II CATG 1 cut(s) 464
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 4 cut(s) 83, 133, 218, 314
HpaII CCGG 2 cut(s) 196, 202
HphI GGTGA 2 cut(s) 178, 755
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 4 cut(s) 99, 106, 396, 568
Hpy188III TCNNGA 3 cut(s) 304, 550, 605
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 2 cut(s) 163, 230
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 3 cut(s) 175, 460, 585
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 221, 230, 247, 457, 577
HpyF3I CTNAG 5 cut(s) 357, 378, 393, 699, 726
HpySE526I ACGT 1 cut(s) 337
Hsp92I GRCGYC 2 cut(s) 75, 384
Hsp92II CATG 1 cut(s) 464
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 430, 544, 563, 689, 705
LguI GCTCTTC 1 cut(s) 231
LmnI GCTCC 2 cut(s) 99, 107
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 775
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 432, 546, 565, 691, 707
MboI GATC 5 cut(s) 430, 544, 563, 689, 705
MboII GAAGA 2 cut(s) 248, 485
MflI RGATCY 1 cut(s) 705
MhlI GDGCHC 4 cut(s) 104, 243, 560, 722
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 3 cut(s) 107, 298, 645
MluI ACGCGT 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 2 cut(s) 142, 212
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 6 cut(s) 32, 89, 118, 142, 612, 679
Mox20I TGGCCA 1 cut(s) 498
Mph1103I ATGCAT 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 53
MscI TGGCCA 1 cut(s) 498
MseI TTAA 1 cut(s) 297
MslI CAYNNNNRTG 1 cut(s) 444
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 2 cut(s) 196, 202
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 355
MvnI CGCG 3 cut(s) 90, 131, 158
MwoI GCNNNNNNNGC 6 cut(s) 149, 221, 230, 247, 457, 577
NciI CCSGG 1 cut(s) 202
NdeII GATC 5 cut(s) 430, 544, 563, 689, 705
NlaIII CATG 1 cut(s) 464
NlaIV GGNNCC 1 cut(s) 200
NsiI ATGCAT 1 cut(s) 587
NspI RCATGY 1 cut(s) 464
PciSI GCTCTTC 1 cut(s) 231
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 87, 334
PctI GAATGC 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 2 cut(s) 83, 314
PkrI GCNGC 3 cut(s) 130, 174, 322
PleI GAGTC 2 cut(s) 141, 212
PpsI GAGTC 2 cut(s) 141, 212
Psp124BI GAGCTC 2 cut(s) 104, 243
Psp1406I AACGTT 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 3 cut(s) 13, 198, 599
PstI CTGCAG 1 cut(s) 177
PstNI CAGNNNCTG 2 cut(s) 400, 494
PsuI RGATCY 1 cut(s) 705
RsaI GTAC 2 cut(s) 185, 283
RsaNI GTAC 2 cut(s) 184, 282
RseI CAYNNNNRTG 1 cut(s) 444
SacI GAGCTC 2 cut(s) 104, 243
SapI GCTCTTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 3 cut(s) 129, 173, 321
Sau3AI GATC 5 cut(s) 430, 544, 563, 689, 705
Sau96I GGNCC 3 cut(s) 13, 198, 599
SchI GAGTC 2 cut(s) 142, 212
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 4 cut(s) 104, 243, 560, 722
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 173
SinI GGWCC 2 cut(s) 198, 599
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 3 cut(s) 107, 298, 645
SsiI CCGC 6 cut(s) 88, 129, 152, 164, 222, 320
SspMI CTAG 1 cut(s) 775
SstI GAGCTC 2 cut(s) 104, 243
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 138, 434
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 340
TaqI TCGA 5 cut(s) 33, 47, 81, 574, 692
TasI AATT 3 cut(s) 107, 298, 645
TatI WGTACW 1 cut(s) 183
TauI GCSGC 2 cut(s) 131, 323
TfiI GAWTC 2 cut(s) 83, 314
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 419
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 486
TspRI CASTG 1 cut(s) 419
VpaK11BI GGWCC 2 cut(s) 198, 599
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 1 cut(s) 775
Zsp2I ATGCAT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.