Rroxscaffold_6G00408760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010508
Physical Location & Seq
Reverse (-)
31255382 .. 31258549
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408760.1

Sequence Viewer

Length: 1455 bp
ATGGAGGTTCAGACCCAGAGCCTCTCGGGTTTCGACCATCTCCAGTCGAAGCCATTCTCCTACTCCCCCAACGGCGTCCTCGATTCCCGCGACTTTCGGAGCTCCGAATTGTCCCCGAAATGCCAGGCCGCGAGTCCCAAGGCGAGTGAGGTGGACGCGCCGGGGGAGAGCGGTCCGAGTCGAAAGCGGCGGTGGCAAGCGATAGACTATGCGAGACTGAATCACAGTACCATTTCTTTCTTTAATTTCTTGAAGCCCGAATCTGCGGCTTCACGCTGGAAACGTTATAGCTTGAATGCTTCTAAGGTGGGCAAGGGATTGTCTAAAGACGCCAAAGCTCAGAAACTGGCTTTTCAGCACTGGATTGAAGCGATTGATCCAAGGCACAGGTATGGGCATAGCTTGCATGTGTATTATGAAGAATGGTGTAAAGCAGATGCTGGTCAGCCATTTTTTTACTGGTTGGATATAGGAGATGGCAAAGAGCTTGATCTCAAGAAGTGCCCAAGATCAGAGCTTCGACAGCAATGCATTAGATATCTTGGACCTCATGAGAGAATACATTACGAGTACGTTGTTGCTGATGGGCAAATTATTCACTCACAAACTGGAGAGCTACTCGATACAAATGAGGGATCGAAAGCTAAGTGGATCTTTGTTATGAGCACCACTAAGAAACTCTATGCTGGTGAAAAAAAGAAAGGAGTTTTCCATCATTCTAGCTTCTTGGCTGGAGGAGCAACAATAGCTGCCGGAAGGCTAGCAGCAACGCATGGAATTCTGAAGTCCATTTCTGCATATAGTGGACACTATCGACCAACTGATGACCGTCTTGATAGCTTCTTATCCTTTCTCAAGGAAAATGGTGTGAACCTTGATGAAGTTGAGATACGCAAGGCAAATGAAGATTCAGATAAGTATGATGATGGCAAGTCTATTAAAAGGGGGACTACATTTGAATTTCCAACCAACTCTGAAGCTCCTAGTGAACCTGAGATACCAGAAGAAGTTGAGAAAACCGAAGTCTTAGAATCCACTGAAGTCCCCAAGACAGACACCAAAACTAATTACCAAAGGACTTTATCTGGCGGTCTTCAGAGTCCAAGAGCAGAAGTGCCTCAGAACAAGATACTGCAGAGGATCAACTCCAAGACGCAAGCCAAATCATACCAACTTGGGCATCAACTCTCCCTCAAGTGGTCAACGGGAGCTGGACCAAGAATTGGTTGTGTTGCTGATTATCCAGTAGAACTGAGAACGCAGGCCTTGGAGTTTGTCAATCTATCTCCAAGATCACCCCAAACACCATCCTATAGGAGGTTTGCTGGTCTCGCATCACCTACATCTGGTCTCGCGTCACCTACATCTGGTCTCGCACCTACATCTAGTCTCGCATCGCCTACATCCATTTCCACCTCAGATATCAACCATGGTGATTTGACCTCTGGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

484

Amino Acids

53.69

Weight (kDa)

8.54

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1223
AccBSI CCGCTC 1 cut(s) 171
AccII CGCG 4 cut(s) 90, 131, 158, 1355
AciI CCGC 7 cut(s) 88, 129, 171, 187, 190, 266, 1089
AclI AACGTT 1 cut(s) 283
AclWI GGATC 4 cut(s) 371, 643, 659, 1148
AcsI RAATTY 2 cut(s) 777, 959
AcuI CTGAAG 4 cut(s) 803, 996, 1059, 1079
AcyI GRCGYC 2 cut(s) 75, 330
AfaI GTAC 2 cut(s) 229, 572
AfiI CCNNNNNNNGG 5 cut(s) 1197, 1223, 1317, 1346, 1367
AgsI TTSAA 4 cut(s) 253, 295, 368, 959
AjnI CCWGG 1 cut(s) 123
Alw21I GWGCWC 2 cut(s) 104, 668
Alw26I GTCTC 5 cut(s) 208, 1334, 1355, 1376, 1394
AlwI GGATC 4 cut(s) 371, 643, 659, 1148
AlwNI CAGNNNCTG 2 cut(s) 346, 440
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 2 cut(s) 126, 1263
ApeKI GCWGC 2 cut(s) 749, 764
ApoI RAATTY 2 cut(s) 777, 959
Asp700I GAANNNNTTC 1 cut(s) 53
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 3 cut(s) 173, 545, 1214
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 5 cut(s) 701, 1287, 1329, 1350, 1445
AsuNHI GCTAGC 1 cut(s) 760
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 3 cut(s) 173, 545, 1214
BaeGI GKGCMC 1 cut(s) 506
BanII GRGCYC 1 cut(s) 104
BarI GAAGNNNNNNTAC 2 cut(s) 873, 905
BbsI GAAGAC 1 cut(s) 1085
Bbv12I GWGCWC 2 cut(s) 104, 668
BbvI GCAGC 2 cut(s) 736, 776
BccI CCATC 6 cut(s) 45, 470, 578, 720, 920, 1315
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 1 cut(s) 125
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 5 cut(s) 208, 1334, 1355, 1376, 1394
BfaI CTAG 4 cut(s) 720, 761, 984, 1386
BfmI CTRYAG 2 cut(s) 1133, 1312
BisI GCNGC 5 cut(s) 129, 188, 267, 750, 765
BlsI GCNGC 5 cut(s) 130, 189, 268, 751, 766
Bme1390I CCNGG 2 cut(s) 125, 162
Bme18I GGWCC 3 cut(s) 173, 545, 1214
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 173, 545, 1214
BmrFI CCNGG 2 cut(s) 125, 162
BmsI GCATC 4 cut(s) 427, 1189, 1343, 1403
BmtI GCTAGC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 1085
BplI GAGNNNNNCTC 2 cut(s) 603, 635
BpmI CTGGAG 3 cut(s) 26, 630, 753
BpuEI CTTGAG 3 cut(s) 479, 839, 1178
BpuMI CCSGG 1 cut(s) 162
BsaHI GRCGYC 2 cut(s) 75, 330
BsaI GGTCTC 3 cut(s) 1334, 1355, 1376
BsaJI CCNNGG 5 cut(s) 138, 161, 380, 1266, 1429
Bsc4I CCNNNNNNNGG 5 cut(s) 1197, 1223, 1317, 1346, 1367
Bse1I ACTGG 6 cut(s) 43, 351, 365, 464, 613, 1244
Bse3DI GCAATG 1 cut(s) 533
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 5 cut(s) 138, 161, 380, 1266, 1429
BseGI GGATG 2 cut(s) 1307, 1403
BseLI CCNNNNNNNGG 5 cut(s) 1197, 1223, 1317, 1346, 1367
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 5 cut(s) 353, 984, 1133, 1244, 1431
BseNI ACTGG 6 cut(s) 43, 351, 365, 464, 613, 1244
BseRI GAGGAG 1 cut(s) 750
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 2 cut(s) 736, 776
Bsh1236I CGCG 4 cut(s) 90, 131, 158, 1355
BshFI GGCC 2 cut(s) 128, 1265
BsiHKAI GWGCWC 2 cut(s) 104, 668
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 161, 753
BslFI GGGAC 4 cut(s) 97, 120, 961, 1028
BslI CCNNNNNNNGG 5 cut(s) 1197, 1223, 1317, 1346, 1367
BsmAI GTCTC 5 cut(s) 208, 1334, 1355, 1376, 1394
BsmFI GGGAC 4 cut(s) 97, 120, 961, 1028
BsmI GAATGC 1 cut(s) 301
BsnI GGCC 2 cut(s) 128, 1265
Bso31I GGTCTC 3 cut(s) 1334, 1355, 1376
BsoBI CYCGRG 1 cut(s) 25
Bsp1286I GDGCHC 3 cut(s) 104, 506, 668
Bsp143I GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
Bsp19I CCATGG 1 cut(s) 1429
BspACI CCGC 7 cut(s) 88, 129, 171, 187, 190, 266, 1089
BspANI GGCC 2 cut(s) 128, 1265
BspCNI CTCAG 5 cut(s) 352, 985, 1132, 1245, 1430
BspFNI CGCG 4 cut(s) 90, 131, 158, 1355
BspHI TCATGA 1 cut(s) 550
BspMAI CTGCAG 1 cut(s) 1137
BspOI GCTAGC 1 cut(s) 764
BspPI GGATC 4 cut(s) 371, 643, 659, 1148
BspTNI GGTCTC 3 cut(s) 1334, 1355, 1376
BsrBI CCGCTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 533
BsrI ACTGG 6 cut(s) 43, 351, 365, 464, 613, 1244
BssECI CCNNGG 5 cut(s) 138, 161, 380, 1266, 1429
BssMI GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
BssNI GRCGYC 2 cut(s) 75, 330
BssT1I CCWWGG 4 cut(s) 138, 380, 1266, 1429
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 227, 830
BstACI GRCGYC 2 cut(s) 75, 330
BstAPI GCANNNNNTGC 1 cut(s) 403
BstC8I GCNNGC 5 cut(s) 198, 404, 762, 1158, 1263
BstDEI CTNAG 9 cut(s) 303, 339, 645, 672, 993, 1027, 1119, 1253, 1417
BstDSI CCRYGG 1 cut(s) 1429
BstENI CCTNNNNNAGG 1 cut(s) 1315
BstF5I GGATG 2 cut(s) 1307, 1403
BstFNI CGCG 4 cut(s) 90, 131, 158, 1355
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 7 cut(s) 379, 493, 512, 638, 654, 1143, 1295
BstMAI GTCTC 5 cut(s) 208, 1334, 1355, 1376, 1394
BstMBI GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
BstMWI GCNNNNNNNGC 6 cut(s) 193, 403, 523, 737, 746, 1331
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 123, 160
BstSFI CTRYAG 2 cut(s) 1133, 1312
BstSLI GKGCMC 1 cut(s) 506
BstUI CGCG 4 cut(s) 90, 131, 158, 1355
BstV1I GCAGC 2 cut(s) 736, 776
BstV2I GAAGAC 1 cut(s) 1085
BstX2I RGATCY 1 cut(s) 651
BstYI RGATCY 1 cut(s) 651
BsuRI GGCC 2 cut(s) 128, 1265
BtgI CCRYGG 1 cut(s) 1429
BtgZI GCGATG 1 cut(s) 1380
BtsCI GGATG 2 cut(s) 1307, 1403
BtsIMutI CAGTG 2 cut(s) 358, 1035
Cac8I GCNNGC 5 cut(s) 198, 404, 762, 1158, 1263
CaiI CAGNNNCTG 2 cut(s) 346, 440
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 3 cut(s) 173, 545, 1214
CpoI CGGWCCG 1 cut(s) 173
CseI GACGC 5 cut(s) 64, 164, 338, 1162, 1344
Csp6I GTAC 2 cut(s) 228, 571
CspI CGGWCCG 1 cut(s) 173
CviAII CATG 4 cut(s) 407, 551, 773, 1430
CviQI GTAC 2 cut(s) 228, 571
DdeI CTNAG 9 cut(s) 303, 339, 645, 672, 993, 1027, 1119, 1253, 1417
DpnI GATC 7 cut(s) 378, 492, 511, 637, 653, 1142, 1294
DpnII GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 4 cut(s) 138, 380, 1266, 1429
Eco147I AGGCCT 1 cut(s) 1265
Eco24I GRGCYC 1 cut(s) 104
Eco31I GGTCTC 3 cut(s) 1334, 1355, 1376
Eco32I GATATC 2 cut(s) 539, 1423
Eco47I GGWCC 3 cut(s) 173, 545, 1214
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 4 cut(s) 803, 996, 1059, 1079
Eco88I CYCGRG 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 1315
EcoRI GAATTC 1 cut(s) 777
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 2 cut(s) 539, 1423
EcoT14I CCWWGG 4 cut(s) 138, 380, 1266, 1429
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 4 cut(s) 138, 380, 1266, 1429
FaeI CATG 4 cut(s) 410, 554, 776, 1433
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 4 cut(s) 97, 120, 961, 1028
FatI CATG 4 cut(s) 406, 550, 772, 1429
FauI CCCGC 1 cut(s) 95
Fnu4HI GCNGC 5 cut(s) 129, 188, 267, 750, 765
FokI GGATG 2 cut(s) 1294, 1390
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 5 cut(s) 129, 188, 267, 750, 765
FspBI CTAG 4 cut(s) 720, 761, 984, 1386
GlaI GCGC 1 cut(s) 159
GluI GCNGC 5 cut(s) 129, 188, 267, 750, 765
GsuI CTGGAG 3 cut(s) 26, 630, 753
HaeIII GGCC 2 cut(s) 128, 1265
HapII CCGG 2 cut(s) 161, 753
HgaI GACGC 5 cut(s) 64, 164, 338, 1162, 1344
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 2 cut(s) 75, 330
Hin1II CATG 4 cut(s) 410, 554, 776, 1433
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 1203
HindII GTYRAC 1 cut(s) 1203
HinfI GANTC 8 cut(s) 83, 133, 178, 220, 260, 908, 1031, 1099
HpaII CCGG 2 cut(s) 161, 753
HphI GGTGA 5 cut(s) 701, 1287, 1329, 1350, 1445
Hpy166II GTNNAC 5 cut(s) 154, 806, 871, 989, 1203
Hpy188III TCNNGA 4 cut(s) 250, 496, 551, 833
Hpy8I GTNNAC 5 cut(s) 154, 806, 871, 989, 1203
HpyAV CCTTC 1 cut(s) 750
HpyCH4III ACNGT 2 cut(s) 227, 830
HpyCH4IV ACGT 2 cut(s) 283, 573
HpyCH4V TGCA 4 cut(s) 406, 531, 797, 1135
HpyF10VI GCNNNNNNNGC 6 cut(s) 193, 403, 523, 737, 746, 1331
HpyF3I CTNAG 9 cut(s) 303, 339, 645, 672, 993, 1027, 1119, 1253, 1417
HpySE526I ACGT 2 cut(s) 283, 573
Hsp92I GRCGYC 2 cut(s) 75, 330
Hsp92II CATG 4 cut(s) 410, 554, 776, 1433
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
LmnI GCTCC 5 cut(s) 99, 107, 737, 985, 1208
Lsp1109I GCAGC 2 cut(s) 736, 776
LweI GCATC 4 cut(s) 427, 1189, 1343, 1403
MaeI CTAG 4 cut(s) 720, 761, 984, 1386
MaeII ACGT 2 cut(s) 283, 573
MaeIII GTNAC 1 cut(s) 1356
MalI GATC 7 cut(s) 378, 492, 511, 637, 653, 1142, 1294
MbiI CCGCTC 1 cut(s) 171
MboI GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
MboII GAAGA 4 cut(s) 431, 917, 1016, 1085
MflI RGATCY 1 cut(s) 651
MhlI GDGCHC 3 cut(s) 104, 506, 668
MluCI AATT 7 cut(s) 107, 244, 591, 777, 959, 1066, 1221
MlyI GAGTC 3 cut(s) 142, 187, 1108
MmeI TCCRAC 2 cut(s) 444, 989
Mph1103I ATGCAT 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 2 cut(s) 243, 939
MslI CAYNNNNRTG 1 cut(s) 390
MspI CCGG 2 cut(s) 161, 753
MspR9I CCNGG 2 cut(s) 125, 162
Mva1269I GAATGC 1 cut(s) 301
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 4 cut(s) 90, 131, 158, 1355
MwoI GCNNNNNNNGC 6 cut(s) 193, 403, 523, 737, 746, 1331
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 1429
NdeII GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
NheI GCTAGC 1 cut(s) 760
NlaIII CATG 4 cut(s) 410, 554, 776, 1433
NmuCI GTSAC 1 cut(s) 1356
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 1 cut(s) 410
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 1 cut(s) 1265
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 87, 280
PctI GAATGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 5 cut(s) 83, 220, 260, 908, 1031
PflMI CCANNNNNTGG 1 cut(s) 1223
PkrI GCNGC 5 cut(s) 130, 189, 268, 751, 766
PleI GAGTC 3 cut(s) 141, 186, 1107
PpsI GAGTC 3 cut(s) 141, 186, 1107
Psp124BI GAGCTC 1 cut(s) 104
Psp1406I AACGTT 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspPI GGNCC 3 cut(s) 173, 545, 1214
PsrI GAACNNNNNNTAC 2 cut(s) 981, 1013
PstI CTGCAG 1 cut(s) 1137
PstNI CAGNNNCTG 2 cut(s) 346, 440
PsuI RGATCY 1 cut(s) 651
RsaI GTAC 2 cut(s) 229, 572
RsaNI GTAC 2 cut(s) 228, 571
RseI CAYNNNNRTG 1 cut(s) 390
Rsr2I CGGWCCG 1 cut(s) 173
RsrII CGGWCCG 1 cut(s) 173
SacI GAGCTC 1 cut(s) 104
SaqAI TTAA 2 cut(s) 243, 939
SatI GCNGC 5 cut(s) 129, 188, 267, 750, 765
Sau3AI GATC 7 cut(s) 376, 490, 509, 635, 651, 1140, 1292
Sau96I GGNCC 3 cut(s) 173, 545, 1214
SchI GAGTC 3 cut(s) 142, 187, 1108
ScrFI CCNGG 2 cut(s) 125, 162
SduI GDGCHC 3 cut(s) 104, 506, 668
SfaNI GCATC 4 cut(s) 427, 1189, 1343, 1403
SfcI CTRYAG 2 cut(s) 1133, 1312
SinI GGWCC 3 cut(s) 173, 545, 1214
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 3 cut(s) 494, 854, 1193
SmoI CTYRAG 3 cut(s) 494, 854, 1193
Sse9I AATT 7 cut(s) 107, 244, 591, 777, 959, 1066, 1221
SseBI AGGCCT 1 cut(s) 1265
SsiI CCGC 7 cut(s) 88, 129, 171, 187, 190, 266, 1089
SspMI CTAG 4 cut(s) 720, 761, 984, 1386
SstI GAGCTC 1 cut(s) 104
StuI AGGCCT 1 cut(s) 1265
StyD4I CCNGG 2 cut(s) 123, 160
StyI CCWWGG 4 cut(s) 138, 380, 1266, 1429
TaaI ACNGT 2 cut(s) 227, 830
TaiI ACGT 2 cut(s) 286, 576
TaqI TCGA 8 cut(s) 33, 47, 81, 181, 520, 621, 638, 814
TasI AATT 7 cut(s) 107, 244, 591, 777, 959, 1066, 1221
TauI GCSGC 3 cut(s) 131, 190, 269
TfiI GAWTC 5 cut(s) 83, 220, 260, 908, 1031
Tru1I TTAA 2 cut(s) 243, 939
Tru9I TTAA 2 cut(s) 243, 939
TscAI CASTG 2 cut(s) 365, 1042
TseFI GTSAC 1 cut(s) 1356
TseI GCWGC 2 cut(s) 749, 764
Tsp45I GTSAC 1 cut(s) 1356
TspDTI ATGAA 3 cut(s) 432, 894, 918
TspRI CASTG 2 cut(s) 365, 1042
Van91I CCANNNNNTGG 1 cut(s) 1223
VpaK11BI GGWCC 3 cut(s) 173, 545, 1214
XagI CCTNNNNNAGG 1 cut(s) 1315
XapI RAATTY 2 cut(s) 777, 959
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 4 cut(s) 720, 761, 984, 1386
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.