pycom12g02060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010508
Physical Location & Seq
Forward (+)
1765462 .. 1767658
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02060.4

Sequence Viewer

Length: 1194 bp
ATGGAATTGAATCTGCCACTAAACGATGGAACCGTGTCGGCAAGAATGCTTCTAAGGTTGGCCAGGGGCTGTCCAAAGACGCCAAAGCACAGAAATTGGCTTTTCAGCATTGGATTGAAGCTTGATCCAAGGCATAGGTATGGTCATAGCCTGCATGTGTATTATGAAGAGTGGTCTAAAGCGGATGCTGGTCAGCCATTTTTTTACTGGTTGGATATAGGAGATGGAAAAGATATTGACCTGAAAGAATGCCCGAGATCGAAGCTTCGACAACAATGCATCAAGTATCTTGGACCTTATGAGTACATTGTTGAGGAAGGGAAACTTATTCACTCGCAAACCGAAGAGCTTCTTGATACAAAGGATGGATCGCCGGGAGCTAAGTGGATATTCGTTATGAGCACATCTAAGAAACTCTATGCTGGCGTGAAAAAGAAAGGGGTTTTCCATCATTCTAGCTTCTTGGCTGGAGGGGCAACGATAGCCGCTGGAAGGCTAGAGGCAGAGAAAGGAATTCTGAAGTCAATCTCTGCATATAGCGGGCATTACCGGCCAACAGATGACAGGCTTGATACATTTTTATCAGTTCTCAAGGAAAACGGGGTGAACCTTGATGAAGTTAAGATACGCAAGGCAAGTGACGATTCTGATAGCTACGATGATGGCAAGTCTAATGGCGGGACTACATTTAAAATGACAGCCAACTTTGAAACTCCTCAACCGGAGATTCTAAGCGAAGTTGAGAAAACCCAATCCCCAGAACCAACGGAACTTCCCCAACTGGAAGCCAAACCTGATTACCAAAGGACTTTATCCGGAGGTCTTCATAGTCCAAGACCTGAGGTGCCTAAGAATAAGATACTGGAGAGGATCAACTCCAAGACCTCAGCGAAATCATACCAACTCGGCCATCAACTATCACTCAAGTGGTCAACAGGAGCTGGCCCGAGAATTGGTTGTGTTGCTGACTATCCGGTAGAGCTGAGAACGCAGGCCTTAGAGTTTGTTAACCTATCTCCGAGGTCTCCCCCAACGCCATCCTACAGGCGGTTCCCTGGTCTCGCTTCACCTACATCTGGTCTCGCATCACCTACTTCTGGTCTCGCATCACCTACTTCTGGTCTCGTATCACCTACATCTGGTTTCGCATCACCTACATTGAAGTCCACCTCAGATGTCAATAACGGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

398

Amino Acids

43.67

Weight (kDa)

8.88

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 844
AccIII TCCGGA 1 cut(s) 815
AciI CCGC 5 cut(s) 182, 486, 540, 678, 1048
AclWI GGATC 3 cut(s) 119, 376, 878
AcoI YGGCCR 3 cut(s) 60, 551, 907
AcsI RAATTY 1 cut(s) 513
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 7 cut(s) 492, 953, 1047, 1076, 1097, 1118, 1139
AgsI TTSAA 4 cut(s) 10, 118, 710, 1162
AjnI CCWGG 2 cut(s) 62, 1054
AleI CACNNNNGTG 1 cut(s) 925
AluBI AGCT 8 cut(s) 121, 265, 349, 380, 459, 654, 941, 982
AluI AGCT 8 cut(s) 121, 265, 349, 380, 459, 654, 941, 982
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
AlwI GGATC 3 cut(s) 119, 376, 878
AlwNI CAGNNNCTG 2 cut(s) 69, 941
Ama87I CYCGRG 2 cut(s) 253, 946
Aor13HI TCCGGA 1 cut(s) 815
AoxI GGCC 5 cut(s) 60, 551, 907, 943, 993
ApoI RAATTY 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 293, 944
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 6 cut(s) 616, 1059, 1080, 1101, 1122, 1143
AvaI CYCGRG 2 cut(s) 253, 946
AvaII GGWCC 1 cut(s) 293
AxyI CCTNAGG 1 cut(s) 840
BalI TGGCCA 1 cut(s) 62
BanI GGYRCC 1 cut(s) 844
BarI GAAGNNNNNNTAC 2 cut(s) 609, 641
BbsI GAAGAC 1 cut(s) 815
Bbv12I GWGCWC 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 886
BccI CCATC 7 cut(s) 20, 218, 359, 456, 656, 918, 1045
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BciT130I CCWGG 2 cut(s) 64, 1056
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BfaI CTAG 2 cut(s) 456, 497
BfmI CTRYAG 1 cut(s) 1042
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 3 cut(s) 64, 375, 1056
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 2 cut(s) 253, 946
BmgT120I GGNCC 2 cut(s) 293, 944
BmiI GGNNCC 3 cut(s) 31, 846, 1052
BmrFI CCNGG 3 cut(s) 64, 375, 1056
BmsI GCATC 5 cut(s) 175, 288, 1094, 1115, 1157
BpiI GAAGAC 1 cut(s) 815
BpmI CTGGAG 2 cut(s) 489, 884
Bpu10I CCTNAGC 1 cut(s) 886
BpuEI CTTGAG 2 cut(s) 575, 908
BpuMI CCSGG 1 cut(s) 375
BsaHI GRCGYC 1 cut(s) 80
BsaI GGTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BsaJI CCNNGG 4 cut(s) 63, 128, 1019, 1054
BsaWI WCCGGW 3 cut(s) 721, 815, 973
Bsc4I CCNNNNNNNGG 7 cut(s) 492, 953, 1047, 1076, 1097, 1118, 1139
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 3 cut(s) 212, 786, 867
Bse21I CCTNAGG 1 cut(s) 840
BseAI TCCGGA 1 cut(s) 815
BseBI CCWGG 2 cut(s) 64, 1056
BseDI CCNNGG 4 cut(s) 63, 128, 1019, 1054
BseGI GGATG 3 cut(s) 190, 370, 1037
BseLI CCNNNNNNNGG 7 cut(s) 492, 953, 1047, 1076, 1097, 1118, 1139
BseMII CTCAG 4 cut(s) 831, 900, 974, 1185
BseNI ACTGG 3 cut(s) 212, 786, 867
BseRI GAGGAG 1 cut(s) 705
BshFI GGCC 5 cut(s) 62, 553, 909, 945, 995
BshNI GGYRCC 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 404
BsiHKCI CYCGRG 2 cut(s) 253, 946
BsiSI CCGG 5 cut(s) 374, 550, 722, 816, 974
BslFI GGGAC 1 cut(s) 694
BslI CCNNNNNNNGG 7 cut(s) 492, 953, 1047, 1076, 1097, 1118, 1139
BsmAI GTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BsmFI GGGAC 1 cut(s) 694
BsmI GAATGC 2 cut(s) 51, 254
BsnI GGCC 5 cut(s) 62, 553, 909, 945, 995
Bso31I GGTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BsoBI CYCGRG 2 cut(s) 253, 946
Bsp1286I GDGCHC 1 cut(s) 404
Bsp13I TCCGGA 1 cut(s) 815
Bsp143I GATC 4 cut(s) 124, 257, 368, 870
BspACI CCGC 5 cut(s) 182, 486, 540, 678, 1048
BspANI GGCC 5 cut(s) 62, 553, 909, 945, 995
BspCNI CTCAG 4 cut(s) 832, 899, 975, 1184
BspEI TCCGGA 1 cut(s) 815
BspLI GGNNCC 3 cut(s) 31, 846, 1052
BspPI GGATC 3 cut(s) 119, 376, 878
BspQI GCTCTTC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 844
BspTNI GGTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BsrFI RCCGGY 1 cut(s) 549
BsrI ACTGG 3 cut(s) 212, 786, 867
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 4 cut(s) 63, 128, 1019, 1054
BssMI GATC 4 cut(s) 124, 257, 368, 870
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 2 cut(s) 64, 1056
Bst4CI ACNGT 2 cut(s) 34, 1187
Bst6I CTCTTC 2 cut(s) 162, 339
BstACI GRCGYC 1 cut(s) 80
BstC8I GCNNGC 5 cut(s) 152, 424, 542, 943, 993
BstF5I GGATG 3 cut(s) 190, 370, 1037
BstKTI GATC 4 cut(s) 127, 260, 371, 873
BstMAI GTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
BstMBI GATC 4 cut(s) 124, 257, 368, 870
BstMWI GCNNNNNNNGC 4 cut(s) 473, 482, 550, 988
BstNI CCWGG 2 cut(s) 64, 1056
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 62, 373, 1054
BstSFI CTRYAG 1 cut(s) 1042
BstV2I GAAGAC 1 cut(s) 815
Bsu36I CCTNAGG 1 cut(s) 840
BsuRI GGCC 5 cut(s) 62, 553, 909, 945, 995
BtsCI GGATG 3 cut(s) 190, 370, 1037
Cac8I GCNNGC 5 cut(s) 152, 424, 542, 943, 993
CaiI CAGNNNCTG 2 cut(s) 69, 941
Cfr10I RCCGGY 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 293, 944
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 304
CviAII CATG 1 cut(s) 155
CviQI GTAC 1 cut(s) 304
DpnI GATC 4 cut(s) 126, 259, 370, 872
DpnII GATC 4 cut(s) 124, 257, 368, 870
DraI TTTAAA 1 cut(s) 691
EaeI YGGCCR 3 cut(s) 60, 551, 907
Eam1104I CTCTTC 2 cut(s) 162, 339
EarI CTCTTC 2 cut(s) 162, 339
Eco130I CCWWGG 1 cut(s) 128
Eco147I AGGCCT 1 cut(s) 995
Eco31I GGTCTC 5 cut(s) 1029, 1064, 1085, 1106, 1127
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 539
Eco81I CCTNAGG 1 cut(s) 840
Eco88I CYCGRG 2 cut(s) 253, 946
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 62, 1054
EcoT14I CCWWGG 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 1 cut(s) 158
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 336, 368
FaqI GGGAC 1 cut(s) 694
FatI CATG 1 cut(s) 154
FauI CCCGC 2 cut(s) 533, 671
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 3 cut(s) 197, 377, 1024
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 2 cut(s) 456, 497
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 2 cut(s) 489, 884
HaeIII GGCC 5 cut(s) 62, 553, 909, 945, 995
HapII CCGG 5 cut(s) 374, 550, 722, 816, 974
HgaI GACGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 1 cut(s) 158
HincII GTYRAC 2 cut(s) 933, 1009
HindII GTYRAC 2 cut(s) 933, 1009
HindIII AAGCTT 2 cut(s) 119, 263
HinfI GANTC 3 cut(s) 10, 644, 727
HpaI GTTAAC 1 cut(s) 1009
HpaII CCGG 5 cut(s) 374, 550, 722, 816, 974
HphI GGTGA 6 cut(s) 616, 1059, 1080, 1101, 1122, 1143
Hpy166II GTNNAC 4 cut(s) 607, 933, 1009, 1167
Hpy188I TCNGA 4 cut(s) 519, 649, 1020, 1174
Hpy188III TCNNGA 2 cut(s) 353, 816
Hpy8I GTNNAC 4 cut(s) 607, 933, 1009, 1167
HpyAV CCTTC 2 cut(s) 311, 486
HpyCH4III ACNGT 2 cut(s) 34, 1187
HpyCH4V TGCA 3 cut(s) 154, 279, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 473, 482, 550, 988
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 1 cut(s) 158
Kpn2I TCCGGA 1 cut(s) 815
KspAI GTTAAC 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 124, 257, 368, 870
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 377, 938
LweI GCATC 5 cut(s) 175, 288, 1094, 1115, 1157
MaeI CTAG 2 cut(s) 456, 497
MaeIII GTNAC 1 cut(s) 638
MalI GATC 4 cut(s) 126, 259, 370, 872
MboI GATC 4 cut(s) 124, 257, 368, 870
MboII GAAGA 3 cut(s) 179, 356, 815
MhlI GDGCHC 1 cut(s) 404
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 4 cut(s) 5, 94, 513, 951
MluNI TGGCCA 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 62
Mph1103I ATGCAT 1 cut(s) 281
MroI TCCGGA 1 cut(s) 815
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 62
MseI TTAA 3 cut(s) 621, 690, 1008
MslI CAYNNNNRTG 2 cut(s) 138, 925
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 5 cut(s) 374, 550, 722, 816, 974
MspR9I CCNGG 3 cut(s) 64, 375, 1056
Mva1269I GAATGC 2 cut(s) 51, 254
MvaI CCWGG 2 cut(s) 64, 1056
MwoI GCNNNNNNNGC 4 cut(s) 473, 482, 550, 988
NciI CCSGG 1 cut(s) 375
NdeII GATC 4 cut(s) 124, 257, 368, 870
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 3 cut(s) 31, 846, 1052
NmeAIII GCCGAG 1 cut(s) 885
NmuCI GTSAC 1 cut(s) 638
NsiI ATGCAT 1 cut(s) 281
NspI RCATGY 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 925
PceI AGGCCT 1 cut(s) 995
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 2 cut(s) 51, 254
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 10, 644, 727
PkrI GCNGC 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 62, 1054
PspGI CCWGG 2 cut(s) 62, 1054
PspN4I GGNNCC 3 cut(s) 31, 846, 1052
PspPI GGNCC 2 cut(s) 293, 944
PstNI CAGNNNCTG 2 cut(s) 69, 941
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 138, 925
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 3 cut(s) 621, 690, 1008
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 4 cut(s) 124, 257, 368, 870
Sau96I GGNCC 2 cut(s) 293, 944
ScrFI CCNGG 3 cut(s) 64, 375, 1056
SduI GDGCHC 1 cut(s) 404
SfaNI GCATC 5 cut(s) 175, 288, 1094, 1115, 1157
SfcI CTRYAG 1 cut(s) 1042
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 138, 925
SmlI CTYRAG 2 cut(s) 590, 923
SmoI CTYRAG 2 cut(s) 590, 923
Sse9I AATT 4 cut(s) 5, 94, 513, 951
SseBI AGGCCT 1 cut(s) 995
SsiI CCGC 5 cut(s) 182, 486, 540, 678, 1048
SspMI CTAG 2 cut(s) 456, 497
StuI AGGCCT 1 cut(s) 995
StyD4I CCNGG 3 cut(s) 62, 373, 1054
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 2 cut(s) 34, 1187
TaqI TCGA 2 cut(s) 260, 268
TasI AATT 4 cut(s) 5, 94, 513, 951
TatI WGTACW 1 cut(s) 303
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 3 cut(s) 10, 644, 727
Tru1I TTAA 3 cut(s) 621, 690, 1008
Tru9I TTAA 3 cut(s) 621, 690, 1008
TseFI GTSAC 1 cut(s) 638
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 3 cut(s) 180, 630, 815
TspGWI ACGGA 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 513
XceI RCATGY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 2 cut(s) 456, 497
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.