MD14G1019000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010508
Physical Location & Seq
Forward (+)
1798100 .. 1801220
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1019000.v1.1.491

Sequence Viewer

Length: 1500 bp
ATGGAGGTGGAAACTCAGACACAAACCCTCCCAACCTTCGACAATCCTCCGTTTTCTTACTCCCTCACCGACCTCGGTTCCCGCGACTTCCGCAGCTCTGAAATGTCTGGCGTTGACCCGAAATGCTTGGCCCCCGAAGTGCTTAACGGGGCGCCAGCGAGTGACCCGAATGCCCTGGGGGGCGAGTGGTCGGAGTTGACTGCGGCGGTGAAGGTTCAGAAGGTGTATCGGAGTTACCGCACACGGCGCAGGTTAGCCGACTCCGCCGTCGTCGTTGAAGAATACTGGTGGCAAGCGATAGATGCTTGGAGATTGAACCGCAGCACGATTTCGTTCTTTGATCACGGCGAATCTGTGGCTAAACGTTGGAACCGTGTCGGCAAGAATGCTTCTAAGGTGGGCAAGGGGCTATCCAAAGACGCCAAAGCACAGAAATTGGCTTTTCAGCATTGGATTGAAGCTATTGATCCGAGGCATAGGTATGGCCATAGCTTGCATGTGTATTATGAAGAGTGGTCTAAAGCAGATGCTGGTCAGCCATTTTTTTACTGGTTGGATGTAGGAGATGGAAAAGAGCTTGATCTGAAAGAATGCCCGAGATCGAAGCTTCGACAACAATGCATCAAGTATCTTGGACCTCAAGAGAGAGTGCATTATGAATACATTGTTGAGGAAGGGAAACTTATTCACTCGCAAACTGGAGAGCTTCTTCATACAAAAGATGGATCTCCGGGAGCTAAGTGGATATTCGTTATGAGCACCTCTAAGAGACTCTATGCCGGCTTTAAAAAGAAAGGGGTTTTCCATCATTCTAGCTTCTTGGCTGGAGGAGCGACGATAGCCGCTGGAAGGCTAGAGGCAGAGAATGGAATTCTGAAGTCAATCTCTGCATATAGCGGGCATTACCGGCCAACAGATGACAGGCTTGATACCTTTTTATCAGTTCTCAAGGATAACGGGGTGAACCTAGACGAAGTTCAGATACACAAGGCCGATACTGATTGCTACGATGATGGCAAGTCTAATGGTGGGACTACATTTAGAATGCCAACCAACTTTGAAACTCCTCAACTGGAGATTCCAGAAGTGGAGAAAACCCAATCCGTAGAATCCACTGAACTTCCCCAAGCGGAACCCAAAACTGAGTACAAGAGGACTTTATCCGGTGGTCTTCAAAGTCCAAGAACTGAGGTGCCTCAGAATAAGATACTGCAGAGGATCAACTCCAAGACCTCAGTGAAATCATACCAACTCGGGCATCAGCTATCGCTCAAGTGGTCAACAGGAGCTGGCCCGAGAATCGGTTGTGTTGCTGACTATCCGGTAGAGCTGAGAATGCAGGCCTTAGAGTTTGTTAACCTATCTCCGAGATCTCCCCCAACGCCATCCTACAGGCGGTTCGGTGGTCTCACATCACCTACATCGAATCTCACATCACCAACATCTGGTTTCACATCACATACGTCGAATTCCACCACGGATGTCAATAACGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

500

Amino Acids

55.83

Weight (kDa)

8.09

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 240
AccB1I GGYRCC 2 cut(s) 151, 1192
AccB7I CCANNNNNTGG 1 cut(s) 1445
AccII CGCG 1 cut(s) 84
AclI AACGTT 2 cut(s) 364, 1491
AclWI GGATC 3 cut(s) 461, 733, 1226
AcoI YGGCCR 2 cut(s) 484, 908
AcsI RAATTY 2 cut(s) 870, 1468
AcuI CTGAAG 1 cut(s) 896
AcyI GRCGYC 2 cut(s) 152, 420
AfaI GTAC 1 cut(s) 1148
AfiI CCNNNNNNNGG 4 cut(s) 849, 1301, 1395, 1445
AgsI TTSAA 5 cut(s) 278, 316, 458, 1061, 1175
AjnI CCWGG 1 cut(s) 174
Alw21I GWGCWC 1 cut(s) 761
Alw26I GTCTC 2 cut(s) 763, 1412
AlwI GGATC 3 cut(s) 461, 733, 1226
AlwNI CAGNNNCTG 2 cut(s) 530, 1289
Ama87I CYCGRG 3 cut(s) 595, 1253, 1294
AoxI GGCC 6 cut(s) 129, 484, 908, 990, 1291, 1341
ApeKI GCWGC 2 cut(s) 93, 321
ApoI RAATTY 2 cut(s) 870, 1468
AspLEI GCGC 2 cut(s) 154, 249
AspS9I GGNCC 3 cut(s) 130, 635, 1292
AsuC2I CCSGG 1 cut(s) 732
AsuHPI GGTGA 5 cut(s) 58, 220, 973, 1407, 1428
AvaI CYCGRG 3 cut(s) 595, 1253, 1294
AvaII GGWCC 1 cut(s) 635
BalI TGGCCA 1 cut(s) 486
BanI GGYRCC 2 cut(s) 151, 1192
BarI GAAGNNNNNNTAC 2 cut(s) 966, 998
BbsI GAAGAC 1 cut(s) 1163
Bbv12I GWGCWC 1 cut(s) 761
BbvI GCAGC 2 cut(s) 105, 333
BccI CCATC 5 cut(s) 560, 716, 813, 1007, 1393
BceAI ACGGC 3 cut(s) 251, 260, 361
BcgI CGANNNNNNTGC 2 cut(s) 600, 634
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 732
BcoDI GTCTC 2 cut(s) 763, 1412
BfaI CTAG 3 cut(s) 813, 854, 968
BfmI CTRYAG 2 cut(s) 1211, 1390
BfoI RGCGCY 1 cut(s) 155
BfuAI ACCTGC 1 cut(s) 240
BglII AGATCT 1 cut(s) 1370
BisI GCNGC 4 cut(s) 94, 204, 322, 843
BlsI GCNGC 4 cut(s) 95, 205, 323, 844
Bme1390I CCNGG 2 cut(s) 176, 732
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 3 cut(s) 595, 1253, 1294
BmgT120I GGNCC 3 cut(s) 130, 635, 1292
BmiI GGNNCC 6 cut(s) 79, 132, 153, 371, 1134, 1194
BmrFI CCNGG 2 cut(s) 176, 732
BmsI GCATC 4 cut(s) 292, 517, 630, 1267
BpiI GAAGAC 1 cut(s) 1163
BpmI CTGGAG 3 cut(s) 720, 846, 1094
BpuEI CTTGAG 3 cut(s) 624, 932, 1256
BpuMI CCSGG 1 cut(s) 732
BsaHI GRCGYC 2 cut(s) 152, 420
BsaI GGTCTC 1 cut(s) 1412
BsaJI CCNNGG 5 cut(s) 73, 174, 175, 470, 1476
BsaWI WCCGGW 2 cut(s) 1163, 1321
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 4 cut(s) 849, 1301, 1395, 1445
Bse118I RCCGGY 2 cut(s) 779, 906
Bse1I ACTGG 4 cut(s) 290, 554, 703, 1077
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 5 cut(s) 73, 174, 175, 470, 1476
BseGI GGATG 3 cut(s) 562, 1385, 1486
BseLI CCNNNNNNNGG 4 cut(s) 849, 1301, 1395, 1445
BseMII CTCAG 6 cut(s) 29, 1134, 1179, 1211, 1248, 1322
BseNI ACTGG 4 cut(s) 290, 554, 703, 1077
BseRI GAGGAG 2 cut(s) 843, 1056
BseXI GCAGC 2 cut(s) 105, 333
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 6 cut(s) 131, 486, 910, 992, 1293, 1343
BshNI GGYRCC 2 cut(s) 151, 1192
BsiHKAI GWGCWC 1 cut(s) 761
BsiHKCI CYCGRG 3 cut(s) 595, 1253, 1294
BsiSI CCGG 5 cut(s) 731, 780, 907, 1164, 1322
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 4 cut(s) 849, 1301, 1395, 1445
BsmAI GTCTC 2 cut(s) 763, 1412
BsmFI GGGAC 1 cut(s) 1045
BsmI GAATGC 5 cut(s) 175, 391, 596, 1050, 1341
BsnI GGCC 6 cut(s) 131, 486, 910, 992, 1293, 1343
Bso31I GGTCTC 1 cut(s) 1412
BsoBI CYCGRG 3 cut(s) 595, 1253, 1294
Bsp1286I GDGCHC 1 cut(s) 761
Bsp143I GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
BspANI GGCC 6 cut(s) 131, 486, 910, 992, 1293, 1343
BspCNI CTCAG 6 cut(s) 28, 1135, 1180, 1210, 1247, 1323
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 6 cut(s) 79, 132, 153, 371, 1134, 1194
BspMAI CTGCAG 1 cut(s) 1215
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 3 cut(s) 461, 733, 1226
BspT107I GGYRCC 2 cut(s) 151, 1192
BspTNI GGTCTC 1 cut(s) 1412
BsrFI RCCGGY 2 cut(s) 779, 906
BsrI ACTGG 4 cut(s) 290, 554, 703, 1077
BssAI RCCGGY 2 cut(s) 779, 906
BssECI CCNNGG 5 cut(s) 73, 174, 175, 470, 1476
BssMI GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
BssNI GRCGYC 2 cut(s) 152, 420
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 2 cut(s) 152, 420
BstC8I GCNNGC 7 cut(s) 156, 294, 494, 781, 899, 1291, 1341
BstDSI CCRYGG 1 cut(s) 1476
BstF5I GGATG 3 cut(s) 562, 1385, 1486
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 2 cut(s) 154, 249
BstKTI GATC 7 cut(s) 343, 469, 583, 602, 728, 1221, 1373
BstMAI GTCTC 2 cut(s) 763, 1412
BstMBI GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
BstMWI GCNNNNNNNGC 8 cut(s) 90, 246, 263, 302, 830, 839, 907, 1336
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 2 cut(s) 174, 730
BstSFI CTRYAG 2 cut(s) 1211, 1390
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 105, 333
BstV2I GAAGAC 1 cut(s) 1163
BstX2I RGATCY 2 cut(s) 725, 1370
BstYI RGATCY 2 cut(s) 725, 1370
BsuRI GGCC 6 cut(s) 131, 486, 910, 992, 1293, 1343
BtgI CCRYGG 1 cut(s) 1476
BtsCI GGATG 3 cut(s) 562, 1385, 1486
BtsIMutI CAGTG 2 cut(s) 1113, 1242
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 7 cut(s) 156, 294, 494, 781, 899, 1291, 1341
CaiI CAGNNNCTG 2 cut(s) 530, 1289
CfoI GCGC 2 cut(s) 154, 249
Cfr10I RCCGGY 2 cut(s) 779, 906
Cfr13I GGNCC 3 cut(s) 130, 635, 1292
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 1147
CviAII CATG 1 cut(s) 497
CviQI GTAC 1 cut(s) 1147
DinI GGCGCC 1 cut(s) 153
DpnI GATC 7 cut(s) 342, 468, 582, 601, 727, 1220, 1372
DpnII GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
DraI TTTAAA 1 cut(s) 787
DrdI GACNNNNNNGTC 1 cut(s) 266
DseDI GACNNNNNNGTC 1 cut(s) 266
EaeI YGGCCR 2 cut(s) 484, 908
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 1 cut(s) 253
Eco147I AGGCCT 1 cut(s) 1343
Eco31I GGTCTC 1 cut(s) 1412
Eco47I GGWCC 1 cut(s) 635
Eco57I CTGAAG 1 cut(s) 896
Eco88I CYCGRG 3 cut(s) 595, 1253, 1294
EcoRI GAATTC 2 cut(s) 870, 1468
EcoRII CCWGG 1 cut(s) 174
EcoT22I ATGCAT 1 cut(s) 623
EgeI GGCGCC 1 cut(s) 153
EheI GGCGCC 1 cut(s) 153
FaeI CATG 1 cut(s) 500
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 1 cut(s) 1045
FatI CATG 1 cut(s) 496
FauI CCCGC 2 cut(s) 89, 890
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 4 cut(s) 94, 204, 322, 843
FokI GGATG 3 cut(s) 569, 1372, 1493
Fsp4HI GCNGC 4 cut(s) 94, 204, 322, 843
FspBI CTAG 3 cut(s) 813, 854, 968
GlaI GCGC 2 cut(s) 153, 248
GluI GCNGC 4 cut(s) 94, 204, 322, 843
GsuI CTGGAG 3 cut(s) 720, 846, 1094
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 6 cut(s) 131, 486, 910, 992, 1293, 1343
HapII CCGG 5 cut(s) 731, 780, 907, 1164, 1322
HgaI GACGC 1 cut(s) 428
HhaI GCGC 2 cut(s) 154, 249
Hin1I GRCGYC 2 cut(s) 152, 420
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 2 cut(s) 152, 247
HinP1I GCGC 2 cut(s) 152, 247
HincII GTYRAC 4 cut(s) 115, 198, 1281, 1357
HindII GTYRAC 4 cut(s) 115, 198, 1281, 1357
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 7 cut(s) 260, 350, 771, 1078, 1109, 1299, 1426
HpaI GTTAAC 1 cut(s) 1357
HpaII CCGG 5 cut(s) 731, 780, 907, 1164, 1322
HphI GGTGA 5 cut(s) 58, 220, 973, 1407, 1428
Hpy166II GTNNAC 5 cut(s) 115, 198, 964, 1281, 1357
Hpy188III TCNNGA 2 cut(s) 641, 1082
Hpy8I GTNNAC 5 cut(s) 115, 198, 964, 1281, 1357
Hpy99I CGWCG 4 cut(s) 272, 275, 838, 1468
HpyAV CCTTC 5 cut(s) 46, 205, 214, 668, 843
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 3 cut(s) 364, 1463, 1491
HpyCH4V TGCA 6 cut(s) 496, 621, 652, 890, 1213, 1339
HpyF10VI GCNNNNNNNGC 8 cut(s) 90, 246, 263, 302, 830, 839, 907, 1336
HpySE526I ACGT 3 cut(s) 364, 1463, 1491
Hsp92I GRCGYC 2 cut(s) 152, 420
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 2 cut(s) 152, 247
KasI GGCGCC 1 cut(s) 151
KroI GCCGGC 1 cut(s) 779
KroNI GCCGGC 1 cut(s) 781
Ksp22I TGATCA 1 cut(s) 340
KspAI GTTAAC 1 cut(s) 1357
Kzo9I GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
LmnI GCTCC 3 cut(s) 734, 830, 1286
Lsp1109I GCAGC 2 cut(s) 105, 333
LweI GCATC 4 cut(s) 292, 517, 630, 1267
MaeI CTAG 3 cut(s) 813, 854, 968
MaeII ACGT 3 cut(s) 364, 1463, 1491
MaeIII GTNAC 2 cut(s) 161, 233
MalI GATC 7 cut(s) 342, 468, 582, 601, 727, 1220, 1372
MboI GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
MboII GAAGA 4 cut(s) 290, 521, 701, 1163
MflI RGATCY 2 cut(s) 725, 1370
MhlI GDGCHC 1 cut(s) 761
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 3 cut(s) 434, 870, 1468
MluNI TGGCCA 1 cut(s) 486
Mly113I GGCGCC 1 cut(s) 152
MlyI GAGTC 2 cut(s) 254, 765
MmeI TCCRAC 3 cut(s) 171, 347, 534
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 1 cut(s) 623
MroNI GCCGGC 1 cut(s) 779
MscI TGGCCA 1 cut(s) 486
MseI TTAA 3 cut(s) 144, 786, 1356
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 5 cut(s) 731, 780, 907, 1164, 1322
MspR9I CCNGG 2 cut(s) 176, 732
Mva1269I GAATGC 5 cut(s) 175, 391, 596, 1050, 1341
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 8 cut(s) 90, 246, 263, 302, 830, 839, 907, 1336
NaeI GCCGGC 1 cut(s) 781
NarI GGCGCC 1 cut(s) 152
NciI CCSGG 1 cut(s) 732
NdeII GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
NgoMIV GCCGGC 1 cut(s) 779
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 6 cut(s) 79, 132, 153, 371, 1134, 1194
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 500
PasI CCCWGGG 1 cut(s) 175
PceI AGGCCT 1 cut(s) 1343
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 370
PctI GAATGC 5 cut(s) 175, 391, 596, 1050, 1341
PdiI GCCGGC 1 cut(s) 781
PfeI GAWTC 5 cut(s) 350, 1078, 1109, 1299, 1426
PflMI CCANNNNNTGG 1 cut(s) 1445
PfoI TCCNGGA 1 cut(s) 730
PkrI GCNGC 4 cut(s) 95, 205, 323, 844
PleI GAGTC 2 cut(s) 254, 765
PluTI GGCGCC 1 cut(s) 155
PpsI GAGTC 2 cut(s) 254, 765
Psp1406I AACGTT 2 cut(s) 364, 1491
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 6 cut(s) 79, 132, 153, 371, 1134, 1194
PspPI GGNCC 3 cut(s) 130, 635, 1292
PstI CTGCAG 1 cut(s) 1215
PstNI CAGNNNCTG 2 cut(s) 530, 1289
PsuI RGATCY 2 cut(s) 725, 1370
RsaI GTAC 1 cut(s) 1148
RsaNI GTAC 1 cut(s) 1147
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 3 cut(s) 144, 786, 1356
SatI GCNGC 4 cut(s) 94, 204, 322, 843
Sau3AI GATC 7 cut(s) 340, 466, 580, 599, 725, 1218, 1370
Sau96I GGNCC 3 cut(s) 130, 635, 1292
SchI GAGTC 2 cut(s) 254, 765
ScrFI CCNGG 2 cut(s) 176, 732
SduI GDGCHC 1 cut(s) 761
SfaNI GCATC 4 cut(s) 292, 517, 630, 1267
SfcI CTRYAG 2 cut(s) 1211, 1390
SfoI GGCGCC 1 cut(s) 153
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 639, 947, 1271
SmoI CTYRAG 3 cut(s) 639, 947, 1271
Sse9I AATT 3 cut(s) 434, 870, 1468
SseBI AGGCCT 1 cut(s) 1343
SspDI GGCGCC 1 cut(s) 151
SspMI CTAG 3 cut(s) 813, 854, 968
StuI AGGCCT 1 cut(s) 1343
StyD4I CCNGG 2 cut(s) 174, 730
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 3 cut(s) 367, 1466, 1494
TaqI TCGA 5 cut(s) 39, 602, 610, 1424, 1466
TasI AATT 3 cut(s) 434, 870, 1468
TatI WGTACW 1 cut(s) 1146
TauI GCSGC 2 cut(s) 206, 845
TfiI GAWTC 5 cut(s) 350, 1078, 1109, 1299, 1426
Tru1I TTAA 3 cut(s) 144, 786, 1356
Tru9I TTAA 3 cut(s) 144, 786, 1356
TscAI CASTG 2 cut(s) 1120, 1242
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 93, 321
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 522, 672, 701
TspGWI ACGGA 3 cut(s) 39, 1093, 1493
TspRI CASTG 2 cut(s) 1120, 1242
Van91I CCANNNNNTGG 1 cut(s) 1445
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 2 cut(s) 870, 1468
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 546
XspI CTAG 3 cut(s) 813, 854, 968
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.