Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009889

Physical Location & Seq

Genomic Coordinates Reverse (-)

2302154 .. 2305935

Gene Structure 3782 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1022900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGACGAATTCTCACGACGTGGTGGTCGATGTCGAAGGCGACGGCGTTTTGGCTAATCATCTGAAGCGCGAGAAGCTCATCATTGATACCGACCCTGGAATCGATGATACCATGGCCATCTTAATGGCATTTCAATCACCGGAGTTGGAAATCCTGGGCTTCACAACAATATTTGGTAATGTCACCACAGAAGATGCCACTCGCAATGCCTTGCTTCTGTGTGAGATCGCAGGGCATCCAGGTTTACCTGTGGCGGAGGGAAGTCCTGAGCCTCTAAAGGGTGGAAGGCCATGTGTTGCCGACTTTATACATGGTTCTGATGGGTTGGGGAACTTGCATCTACCTCCTCCAAAAACAAAGAAAATTGAGAAGAATGCTGCTGAATTTTTAGTTGATATGGTCTCTGAATACCCTGGTGAAGTTTCTATACTGGCCCTAGGTCCCTTGACAAACTTAGCATTGGCAGTCAAAAGGGATTCTTCATTCGCGAAAAAGGTGAAAAGGGTGGTCGTACTTGGTGGTTCCTTCTTCGCTCTTGGAAATGTAAATCCTGCCGCAGAAGCAAATATTTATGGAGATCCAGAAGCAGCAGATGTTGTGTTTACGTCTGGGGCTAACATTACCGTTGTTGGAATAAACATTACGACCCAAGTGAAATTTACAGATGATGACCTCCTTCAACTGCGGCAATCCAAAGGAAAGCATACTCAGCTCATATGCGACACATGCAAATTCTACAGAGACTGGCATGTGCAATCTGATGGTGTTTATGGGATTTTCCTTCATGACCCTGTCAGTTTCGTAGCACTAGTCCGTCCTGATCTCTTTACGTACAAGAAGGGCGTTGTGAGGGTTGAGACTCAGGGTATCTGCACCGGGCATACACTGATGGATCAAGGACTAAAAAAATGGAATACAAGCAATCCATGGACAGGCTATTCCCCTGTTGAAGTTGCTTGGACAGTGAATGTGGATGCAGTCCTTGATTATATAAGAAAGCAGTTGATGAAATCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006152 GO:0006213 GO:0006218 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008477 GO:0009056 GO:0009116 GO:0009119 GO:0009164 GO:0009987 GO:0016787 GO:0016798 GO:0016799 GO:0019439 GO:0034641 GO:0034655 GO:0034656 GO:0042278 GO:0042454 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0045437 GO:0046108 GO:0046131 GO:0046133 GO:0046135 GO:0046483 GO:0046700 GO:0047622 GO:0047724 GO:0050263 GO:0055086 GO:0071704 GO:0072521 GO:0072523 GO:0072527 GO:0072529 GO:0072585 GO:1901135 GO:1901136 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658

KEGG Pathways

Metabolic & Signaling

ko00240 ko00760 map00240 map00760

Pfam Domains

Protein Families

IU_nuc_hydro

Protein Analysis

339

Amino Acids

36.73

Weight (kDa)

5.38

Isoelectric Point (pI)

32.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IU_nuc_hydro	PF01156	26 - 328	2.7e-92	Inosine-uridine preferring nucleoside hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36310
fragaria_vesca	FvH4_6g18360 FvH4_6g18360 FvH4_6g18360
malus_domestica	MD12G1022900.v1.1 MD14G1019900.v1.1
prunus_persica	Prupe.7G113700_v2.0.a1 Prupe.7G113700_v2.0.a1 Prupe.7G113700_v2.0.a1 Prupe.7G113700_v2.0.a1
pyrus_communis	pycom12g02140 pycom14g01950
rosa_chinensis	RchiOBHm_Chr3g0472851
rosa_laevigata	RLG00000024051
rosa_roxburghii	Rroxscaffold_6G00408560
rosa_rugosa	Rorug03G0128100
rosa_samantha	Rh3AG180800 Rh3BG207000 Rh3CG204200 Rh3DG168900
rosa_wichuraiana	Rw3G016220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	23
AccII	CGCG	2 cut(s)	69, 488
AciI	CCGC	3 cut(s)	254, 555, 685
AclWI	GGATC	2 cut(s)	572, 900
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	4 cut(s)	7, 383, 656, 731
AcuI	CTGAAG	1 cut(s)	83
AdeI	CACNNNGTG	1 cut(s)	19
AfaI	GTAC	2 cut(s)	513, 833
AfiI	CCNNNNNNNGG	2 cut(s)	278, 932
AgsI	TTSAA	3 cut(s)	134, 680, 950
AhlI	ACTAGT	1 cut(s)	808
AjiI	CACGTC	1 cut(s)	19
AjnI	CCWGG	4 cut(s)	94, 153, 238, 412
AluBI	AGCT	2 cut(s)	76, 712
AluI	AGCT	2 cut(s)	76, 712
Alw26I	GTCTC	3 cut(s)	406, 735, 851
AlwI	GGATC	2 cut(s)	572, 900
AlwNI	CAGNNNCTG	1 cut(s)	744
AoxI	GGCC	3 cut(s)	114, 287, 432
ApeKI	GCWGC	2 cut(s)	377, 587
ApoI	RAATTY	4 cut(s)	7, 383, 656, 731
AspA2I	CCTAGG	1 cut(s)	436
AspLEI	GCGC	1 cut(s)	69
AspS9I	GGNCC	2 cut(s)	433, 440
AsuC2I	CCSGG	1 cut(s)	877
AsuHPI	GGTGA	4 cut(s)	129, 175, 428, 508
AvaII	GGWCC	1 cut(s)	440
AvrII	CCTAGG	1 cut(s)	436
BalI	TGGCCA	1 cut(s)	116
BarI	GAAGNNNNNNTAC	2 cut(s)	411, 443
BbvI	GCAGC	2 cut(s)	364, 599
BccI	CCATC	4 cut(s)	125, 314, 755, 883
BceAI	ACGGC	1 cut(s)	58
BciT130I	CCWGG	4 cut(s)	96, 155, 240, 414
BcnI	CCSGG	1 cut(s)	877
BcoDI	GTCTC	3 cut(s)	406, 735, 851
BcuI	ACTAGT	1 cut(s)	808
BfaI	CTAG	2 cut(s)	437, 809
BfmI	CTRYAG	1 cut(s)	736
BisI	GCNGC	4 cut(s)	378, 555, 588, 686
BlnI	CCTAGG	1 cut(s)	436
BlsI	GCNGC	4 cut(s)	379, 556, 589, 687
Bme1390I	CCNGG	5 cut(s)	96, 155, 240, 414, 877
Bme18I	GGWCC	1 cut(s)	440
BmgBI	CACGTC	1 cut(s)	19
BmgT120I	GGNCC	2 cut(s)	433, 440
BmiI	GGNNCC	2 cut(s)	442, 523
BmrFI	CCNGG	5 cut(s)	96, 155, 240, 414, 877
BmsI	GCATC	4 cut(s)	184, 244, 346, 964
Bpu10I	CCTNAGC	1 cut(s)	267
BpuMI	CCSGG	1 cut(s)	877
Bsa29I	ATCGAT	1 cut(s)	102
BsaAI	YACGTR	1 cut(s)	831
BsaI	GGTCTC	1 cut(s)	406
BsaJI	CCNNGG	6 cut(s)	94, 111, 154, 412, 436, 926
BsaWI	WCCGGW	1 cut(s)	139
Bsc4I	CCNNNNNNNGG	2 cut(s)	278, 932
Bse1I	ACTGG	2 cut(s)	435, 749
Bse3DI	GCAATG	1 cut(s)	211
BseBI	CCWGG	4 cut(s)	96, 155, 240, 414
BseCI	ATCGAT	1 cut(s)	102
BseDI	CCNNGG	6 cut(s)	94, 111, 154, 412, 436, 926
BseGI	GGATG	2 cut(s)	235, 979
BseLI	CCNNNNNNNGG	2 cut(s)	278, 932
BseMI	GCAATG	1 cut(s)	211
BseMII	CTCAG	3 cut(s)	258, 722, 875
BseNI	ACTGG	2 cut(s)	435, 749
BseRI	GAGGAG	1 cut(s)	336
BseXI	GCAGC	2 cut(s)	364, 599
BsgI	GTGCAG	1 cut(s)	856
Bsh1236I	CGCG	2 cut(s)	69, 488
BshFI	GGCC	3 cut(s)	116, 289, 434
BshVI	ATCGAT	1 cut(s)	102
BsiSI	CCGG	2 cut(s)	140, 876
BslFI	GGGAC	1 cut(s)	426
BslI	CCNNNNNNNGG	2 cut(s)	278, 932
BsmAI	GTCTC	3 cut(s)	406, 735, 851
BsmFI	GGGAC	1 cut(s)	426
BsmI	GAATGC	1 cut(s)	379
BsnI	GGCC	3 cut(s)	116, 289, 434
Bso31I	GGTCTC	1 cut(s)	406
Bsp143I	GATC	4 cut(s)	225, 577, 820, 892
Bsp19I	CCATGG	2 cut(s)	111, 926
Bsp68I	TCGCGA	1 cut(s)	488
BspACI	CCGC	3 cut(s)	254, 555, 685
BspANI	GGCC	3 cut(s)	116, 289, 434
BspCNI	CTCAG	3 cut(s)	259, 721, 874
BspDI	ATCGAT	1 cut(s)	102
BspFNI	CGCG	2 cut(s)	69, 488
BspHI	TCATGA	1 cut(s)	784
BspLI	GGNNCC	2 cut(s)	442, 523
BspPI	GGATC	2 cut(s)	572, 900
BspTNI	GGTCTC	1 cut(s)	406
BsrDI	GCAATG	1 cut(s)	211
BsrI	ACTGG	2 cut(s)	435, 749
BssECI	CCNNGG	6 cut(s)	94, 111, 154, 412, 436, 926
BssMI	GATC	4 cut(s)	225, 577, 820, 892
BssT1I	CCWWGG	3 cut(s)	111, 436, 926
Bst2UI	CCWGG	4 cut(s)	96, 155, 240, 414
Bst4CI	ACNGT	2 cut(s)	625, 964
BstBAI	YACGTR	1 cut(s)	831
BstDEI	CTNAG	4 cut(s)	267, 454, 708, 861
BstDSI	CCRYGG	2 cut(s)	111, 926
BstF5I	GGATG	2 cut(s)	235, 979
BstFNI	CGCG	2 cut(s)	69, 488
BstHHI	GCGC	1 cut(s)	69
BstKTI	GATC	4 cut(s)	228, 580, 823, 895
BstMAI	GTCTC	3 cut(s)	406, 735, 851
BstMBI	GATC	4 cut(s)	225, 577, 820, 892
BstMWI	GCNNNNNNNGC	4 cut(s)	73, 560, 709, 726
BstNI	CCWGG	4 cut(s)	96, 155, 240, 414
BstNSI	RCATGY	2 cut(s)	729, 752
BstSCI	CCNGG	5 cut(s)	94, 153, 238, 412, 875
BstSFI	CTRYAG	1 cut(s)	736
BstSNI	TACGTA	1 cut(s)	831
BstUI	CGCG	2 cut(s)	69, 488
BstV1I	GCAGC	2 cut(s)	364, 599
BstX2I	RGATCY	1 cut(s)	577
BstXI	CCANNNNNNTGG	1 cut(s)	124
BstYI	RGATCY	1 cut(s)	577
Bsu15I	ATCGAT	1 cut(s)	102
BsuRI	GGCC	3 cut(s)	116, 289, 434
BsuTUI	ATCGAT	1 cut(s)	102
BtgI	CCRYGG	2 cut(s)	111, 926
BtrI	CACGTC	1 cut(s)	19
BtsCI	GGATG	2 cut(s)	235, 979
BtsIMutI	CAGTG	2 cut(s)	884, 969
BtuMI	TCGCGA	1 cut(s)	488
CaiI	CAGNNNCTG	1 cut(s)	744
CciI	TCATGA	1 cut(s)	784
CfoI	GCGC	1 cut(s)	69
Cfr13I	GGNCC	2 cut(s)	433, 440
ClaI	ATCGAT	1 cut(s)	102
Csp6I	GTAC	2 cut(s)	512, 832
CviAII	CATG	7 cut(s)	112, 291, 311, 726, 749, 785, 927
CviQI	GTAC	2 cut(s)	512, 832
DdeI	CTNAG	4 cut(s)	267, 454, 708, 861
DpnI	GATC	4 cut(s)	227, 579, 822, 894
DpnII	GATC	4 cut(s)	225, 577, 820, 892
DraIII	CACNNNGTG	1 cut(s)	19
DrdI	GACNNNNNNGTC	1 cut(s)	23
DseDI	GACNNNNNNGTC	1 cut(s)	23
EaeI	YGGCCR	1 cut(s)	114
EciI	GGCGGA	1 cut(s)	269
Eco105I	TACGTA	1 cut(s)	831
Eco130I	CCWWGG	3 cut(s)	111, 436, 926
Eco31I	GGTCTC	1 cut(s)	406
Eco47I	GGWCC	1 cut(s)	440
Eco57I	CTGAAG	1 cut(s)	83
EcoO109I	RGGNCCY	1 cut(s)	440
EcoRI	GAATTC	1 cut(s)	7
EcoRII	CCWGG	4 cut(s)	94, 153, 238, 412
EcoT14I	CCWWGG	3 cut(s)	111, 436, 926
ErhI	CCWWGG	3 cut(s)	111, 436, 926
FaeI	CATG	7 cut(s)	115, 294, 314, 729, 752, 788, 930
FalI	AAGNNNNNCTT	2 cut(s)	463, 495
FaqI	GGGAC	1 cut(s)	426
FatI	CATG	7 cut(s)	111, 290, 310, 725, 748, 784, 926
FauNDI	CATATG	1 cut(s)	716
Fnu4HI	GCNGC	4 cut(s)	378, 555, 588, 686
FokI	GGATG	2 cut(s)	222, 986
Fsp4HI	GCNGC	4 cut(s)	378, 555, 588, 686
FspBI	CTAG	2 cut(s)	437, 809
GlaI	GCGC	1 cut(s)	68
GluI	GCNGC	4 cut(s)	378, 555, 588, 686
HaeIII	GGCC	3 cut(s)	116, 289, 434
HapII	CCGG	2 cut(s)	140, 876
HhaI	GCGC	1 cut(s)	69
Hin1II	CATG	7 cut(s)	115, 294, 314, 729, 752, 788, 930
Hin6I	GCGC	1 cut(s)	67
HinP1I	GCGC	1 cut(s)	67
HinfI	GANTC	3 cut(s)	99, 476, 859
HpaII	CCGG	2 cut(s)	140, 876
HphI	GGTGA	4 cut(s)	129, 175, 428, 508
Hpy166II	GTNNAC	2 cut(s)	245, 603
Hpy188I	TCNGA	4 cut(s)	63, 319, 406, 760
Hpy188III	TCNNGA	7 cut(s)	14, 266, 487, 581, 785, 818, 1014
Hpy8I	GTNNAC	2 cut(s)	245, 603
Hpy99I	CGWCG	2 cut(s)	20, 44
HpyAV	CCTTC	6 cut(s)	29, 279, 535, 686, 791, 832
HpyCH4III	ACNGT	2 cut(s)	625, 964
HpyCH4IV	ACGT	3 cut(s)	18, 605, 830
HpyCH4V	TGCA	5 cut(s)	337, 729, 754, 873, 977
HpyF10VI	GCNNNNNNNGC	4 cut(s)	73, 560, 709, 726
HpyF3I	CTNAG	4 cut(s)	267, 454, 708, 861
HpySE526I	ACGT	3 cut(s)	18, 605, 830
Hsp92II	CATG	7 cut(s)	115, 294, 314, 729, 752, 788, 930
HspAI	GCGC	1 cut(s)	67
Kzo9I	GATC	4 cut(s)	225, 577, 820, 892
Lsp1109I	GCAGC	2 cut(s)	364, 599
LweI	GCATC	4 cut(s)	184, 244, 346, 964
MaeI	CTAG	2 cut(s)	437, 809
MaeII	ACGT	3 cut(s)	18, 605, 830
MaeIII	GTNAC	1 cut(s)	181
MalI	GATC	4 cut(s)	227, 579, 822, 894
MboI	GATC	4 cut(s)	225, 577, 820, 892
MboII	GAAGA	4 cut(s)	203, 382, 471, 520
MflI	RGATCY	1 cut(s)	577
MlsI	TGGCCA	1 cut(s)	116
MluCI	AATT	5 cut(s)	7, 363, 383, 656, 731
MluNI	TGGCCA	1 cut(s)	116
MlyI	GAGTC	1 cut(s)	853
MmeI	TCCRAC	2 cut(s)	126, 610
MnlI	CCTC	6 cut(s)	250, 282, 354, 357, 683, 843
Mox20I	TGGCCA	1 cut(s)	116
MscI	TGGCCA	1 cut(s)	116
MseI	TTAA	1 cut(s)	122
MslI	CAYNNNNRTG	1 cut(s)	122
Msp20I	TGGCCA	1 cut(s)	116
MspI	CCGG	2 cut(s)	140, 876
MspR9I	CCNGG	5 cut(s)	96, 155, 240, 414, 877
Mva1269I	GAATGC	1 cut(s)	379
MvaI	CCWGG	4 cut(s)	96, 155, 240, 414
MvnI	CGCG	2 cut(s)	69, 488
MwoI	GCNNNNNNNGC	4 cut(s)	73, 560, 709, 726
NciI	CCSGG	1 cut(s)	877
NcoI	CCATGG	2 cut(s)	111, 926
NdeI	CATATG	1 cut(s)	716
NdeII	GATC	4 cut(s)	225, 577, 820, 892
NlaIII	CATG	7 cut(s)	115, 294, 314, 729, 752, 788, 930
NlaIV	GGNNCC	2 cut(s)	442, 523
NmuCI	GTSAC	1 cut(s)	181
NruI	TCGCGA	1 cut(s)	488
NspI	RCATGY	2 cut(s)	729, 752
PagI	TCATGA	1 cut(s)	784
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PctI	GAATGC	1 cut(s)	379
PfeI	GAWTC	2 cut(s)	99, 476
PflFI	GACNNNGTC	1 cut(s)	791
PkrI	GCNGC	4 cut(s)	379, 556, 589, 687
PleI	GAGTC	1 cut(s)	853
PpsI	GAGTC	1 cut(s)	853
Ppu21I	YACGTR	1 cut(s)	831
PpuMI	RGGWCCY	1 cut(s)	440
Psp5II	RGGWCCY	1 cut(s)	440
Psp6I	CCWGG	4 cut(s)	94, 153, 238, 412
PspGI	CCWGG	4 cut(s)	94, 153, 238, 412
PspN4I	GGNNCC	2 cut(s)	442, 523
PspPI	GGNCC	2 cut(s)	433, 440
PspPPI	RGGWCCY	1 cut(s)	440
PstNI	CAGNNNCTG	1 cut(s)	744
PsuI	RGATCY	1 cut(s)	577
PsyI	GACNNNGTC	1 cut(s)	791
RruI	TCGCGA	1 cut(s)	488
RsaI	GTAC	2 cut(s)	513, 833
RsaNI	GTAC	2 cut(s)	512, 832
RseI	CAYNNNNRTG	1 cut(s)	122
SaqAI	TTAA	1 cut(s)	122
SatI	GCNGC	4 cut(s)	378, 555, 588, 686
Sau3AI	GATC	4 cut(s)	225, 577, 820, 892
Sau96I	GGNCC	2 cut(s)	433, 440
SchI	GAGTC	1 cut(s)	853
ScrFI	CCNGG	5 cut(s)	96, 155, 240, 414, 877
SfaNI	GCATC	4 cut(s)	184, 244, 346, 964
SfcI	CTRYAG	1 cut(s)	736
SinI	GGWCC	1 cut(s)	440
SmiMI	CAYNNNNRTG	1 cut(s)	122
SnaBI	TACGTA	1 cut(s)	831
SpeI	ACTAGT	1 cut(s)	808
Sse9I	AATT	5 cut(s)	7, 363, 383, 656, 731
SsiI	CCGC	3 cut(s)	254, 555, 685
SspI	AATATT	2 cut(s)	171, 568
SspMI	CTAG	2 cut(s)	437, 809
StyD4I	CCNGG	5 cut(s)	94, 153, 238, 412, 875
StyI	CCWWGG	3 cut(s)	111, 436, 926
TaaI	ACNGT	2 cut(s)	625, 964
TaiI	ACGT	3 cut(s)	21, 608, 833
TaqI	TCGA	3 cut(s)	27, 33, 102
TasI	AATT	5 cut(s)	7, 363, 383, 656, 731
TauI	GCSGC	2 cut(s)	557, 688
TfiI	GAWTC	2 cut(s)	99, 476
Tru1I	TTAA	1 cut(s)	122
Tru9I	TTAA	1 cut(s)	122
TscAI	CASTG	2 cut(s)	891, 969
TseFI	GTSAC	1 cut(s)	181
TseI	GCWGC	2 cut(s)	377, 587
Tsp45I	GTSAC	1 cut(s)	181
TspDTI	ATGAA	2 cut(s)	471, 773
TspGWI	ACGGA	1 cut(s)	803
TspRI	CASTG	2 cut(s)	891, 969
Tth111I	GACNNNGTC	1 cut(s)	791
VpaK11BI	GGWCC	1 cut(s)	440
XapI	RAATTY	4 cut(s)	7, 383, 656, 731
XceI	RCATGY	2 cut(s)	729, 752
XmaJI	CCTAGG	1 cut(s)	436
XspI	CTAG	2 cut(s)	437, 809

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.