MD12G1024700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014262
Physical Location & Seq
Reverse (-)
2653080 .. 2653961
882 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024700.v1.1.491

Sequence Viewer

Length: 882 bp
ATGCCACAAGTTGATTTGGAAACACTAGTTTCGGCGTGCGCTGGTGGCTCAATGGATCGGAAAATCGCCTGCGAGACGCAGGCCGACGCCACCACCGCCGGACACGGCCGGCCGGAGGATGACGTCGAGGAACAGGAGGTTCCGCCGGATTTCCCGCCGGAATCCTTCTGGCTCTCGAAAGACGCAGAGTACGACTGGTTCGACCGCAACGCCTTCTACGAGCGCAAAGACTCCACCAAAGGAAACTCCAACGCTAATAACTTGAATTCGAATCAAAATTCGCACTCGAATTCAAACTCGCAGCGGTTTTCGATGAACTTGAAGTCGAAGGCGGCGATCATCGGGCTTCCCAAGCCGCAGAAGCACAACTACACCGACACGAAGAATCGGCGGCACTGCAAGGCCGGAAACACGAGGCTGTTTCCGAAACGGTCCGGATCCGTCGGAAAATCGGACGCTCCGATGATCGAGCCGTCGTCTCCGAAGGTCTCGTGTATGGGGAGGGTGAGATCGAAGAGGGATCGGAAGAAGCGTCGGGTCAGGAATCGGCACAGACAGTCGGCCGAGACGTCCATAGAAAAGTCCGTTAAACCGGTTTCCGAGAGACGAAAACTCGGTTTCTTCGCGAGTTTTCGGGCGATTTTTCGTCACGGCGGACACCGGGACAAATCGGCGAAAACGCCGTTCGTGGACGACTTGCCTCCGAGAAACAGCAGCGTGAAGTCGACGAGAGCGCGCGACAGAGCGGCAGCGGACGATGTCGACTCGCTTCCAAGACACAGCGTGGAGAGCGAACCGCCCGGTTTGGGTGGCATGAAGCGGTTCGTTTCGGGTCGGAGGTCCGGGTCGTGGGTGGGCGATGGCGGTATCGACGTTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

32.6

Weight (kDa)

9.94

Isoelectric Point (pI)

60.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014262)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36220 AT3G52710
fragaria_vesca FvH4_6g18850
malus_domestica MD12G1024700.v1.1 MD14G1023600.v1.1
prunus_persica Prupe.7G109200_v2.0.a1
pyrus_communis pycom12g02440 pycom14g02250
rosa_laevigata RLG00000023978
rosa_multiflora Rmu_sc0005561.1_g000001
rosa_roxburghii Rroxscaffold_6G00407720
rosa_rugosa Rorug03G0136100
rosa_samantha Rh3AG186500 Rh3BG215100 Rh3CG211800 Rh3DG211000
rosa_wichuraiana Rw3G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 126, 572
AccBSI CCGCTC 1 cut(s) 746
AccI GTMKAC 2 cut(s) 725, 762
AccII CGCG 3 cut(s) 626, 736, 738
AccIII TCCGGA 1 cut(s) 434
AclWI GGATC 4 cut(s) 63, 432, 445, 528
AcoI YGGCCR 3 cut(s) 106, 110, 561
AcsI RAATTY 3 cut(s) 265, 277, 289
AcyI GRCGYC 3 cut(s) 87, 123, 569
AdeI CACNNNGTG 1 cut(s) 784
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 115, 806, 849
AgeI ACCGGT 1 cut(s) 592
AgsI TTSAA 3 cut(s) 265, 294, 322
AhlI ACTAGT 1 cut(s) 25
Alw26I GTCTC 5 cut(s) 68, 483, 493, 560, 598
AlwI GGATC 4 cut(s) 63, 432, 445, 528
Aor13HI TCCGGA 1 cut(s) 434
AoxI GGCC 5 cut(s) 81, 106, 110, 402, 561
ApeKI GCWGC 3 cut(s) 301, 714, 749
ApoI RAATTY 3 cut(s) 265, 277, 289
AsiGI ACCGGT 1 cut(s) 592
Asp700I GAANNNNTTC 1 cut(s) 821
AspLEI GCGC 4 cut(s) 41, 225, 736, 738
AspS9I GGNCC 2 cut(s) 432, 840
AsuC2I CCSGG 3 cut(s) 662, 801, 844
AsuHPI GGTGA 1 cut(s) 517
AsuII TTCGAA 1 cut(s) 269
AvaII GGWCC 2 cut(s) 432, 840
BamHI GGATCC 1 cut(s) 437
BarI GAAGNNNNNNTAC 2 cut(s) 353, 385
BauI CACGAG 2 cut(s) 412, 490
BbvI GCAGC 3 cut(s) 313, 726, 761
BccI CCATC 1 cut(s) 854
BceAI ACGGC 4 cut(s) 121, 457, 667, 667
BcnI CCSGG 3 cut(s) 662, 801, 844
BcoDI GTCTC 5 cut(s) 68, 483, 493, 560, 598
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 1 cut(s) 26
BisI GCNGC 7 cut(s) 302, 333, 356, 392, 715, 747, 750
BlsI GCNGC 7 cut(s) 303, 334, 357, 393, 716, 748, 751
Bme1390I CCNGG 3 cut(s) 662, 801, 844
Bme18I GGWCC 2 cut(s) 432, 840
BmgT120I GGNCC 2 cut(s) 432, 840
BmiI GGNNCC 2 cut(s) 141, 439
BmrFI CCNGG 3 cut(s) 662, 801, 844
Bpu14I TTCGAA 1 cut(s) 269
BpuMI CCSGG 3 cut(s) 662, 801, 844
BsaHI GRCGYC 3 cut(s) 87, 123, 569
BsaI GGTCTC 1 cut(s) 493
BsaWI WCCGGW 2 cut(s) 434, 592
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 806, 849
Bse118I RCCGGY 2 cut(s) 108, 592
Bse1I ACTGG 1 cut(s) 200
BseAI TCCGGA 1 cut(s) 434
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 3 cut(s) 115, 806, 849
BseNI ACTGG 1 cut(s) 200
BsePI GCGCGC 1 cut(s) 734
BseX3I CGGCCG 3 cut(s) 106, 110, 561
BseXI GCAGC 3 cut(s) 313, 726, 761
Bsh1236I CGCG 3 cut(s) 626, 736, 738
Bsh1285I CGRYCG 4 cut(s) 109, 113, 205, 564
BshFI GGCC 5 cut(s) 83, 108, 112, 404, 563
BshTI ACCGGT 1 cut(s) 592
BsiEI CGRYCG 4 cut(s) 109, 113, 205, 564
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 3 cut(s) 115, 806, 849
BsmAI GTCTC 5 cut(s) 68, 483, 493, 560, 598
BsmBI CGTCTC 4 cut(s) 68, 483, 560, 598
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 5 cut(s) 83, 108, 112, 404, 563
Bso31I GGTCTC 1 cut(s) 493
Bsp119I TTCGAA 1 cut(s) 269
Bsp13I TCCGGA 1 cut(s) 434
Bsp143I GATC 6 cut(s) 55, 336, 437, 465, 509, 520
Bsp68I TCGCGA 1 cut(s) 626
BspANI GGCC 5 cut(s) 83, 108, 112, 404, 563
BspEI TCCGGA 1 cut(s) 434
BspFNI CGCG 3 cut(s) 626, 736, 738
BspLI GGNNCC 2 cut(s) 141, 439
BspPI GGATC 4 cut(s) 63, 432, 445, 528
BspT104I TTCGAA 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 493
BsrBI CCGCTC 1 cut(s) 746
BsrFI RCCGGY 2 cut(s) 108, 592
BsrI ACTGG 1 cut(s) 200
BssAI RCCGGY 2 cut(s) 108, 592
BssHII GCGCGC 1 cut(s) 734
BssMI GATC 6 cut(s) 55, 336, 437, 465, 509, 520
BssNI GRCGYC 3 cut(s) 87, 123, 569
BssSI CACGAG 2 cut(s) 412, 490
Bst2BI CACGAG 2 cut(s) 412, 490
Bst4CI ACNGT 2 cut(s) 432, 558
Bst6I CTCTTC 1 cut(s) 509
BstACI GRCGYC 3 cut(s) 87, 123, 569
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 5 cut(s) 37, 70, 81, 110, 736
BstF5I GGATG 1 cut(s) 124
BstFNI CGCG 3 cut(s) 626, 736, 738
BstHHI GCGC 4 cut(s) 41, 225, 736, 738
BstKTI GATC 6 cut(s) 58, 339, 440, 468, 512, 523
BstMAI GTCTC 5 cut(s) 68, 483, 493, 560, 598
BstMBI GATC 6 cut(s) 55, 336, 437, 465, 509, 520
BstMCI CGRYCG 4 cut(s) 109, 113, 205, 564
BstMWI GCNNNNNNNGC 5 cut(s) 45, 95, 352, 361, 789
BstSCI CCNGG 3 cut(s) 660, 799, 842
BstUI CGCG 3 cut(s) 626, 736, 738
BstV1I GCAGC 3 cut(s) 313, 726, 761
BstX2I RGATCY 1 cut(s) 437
BstYI RGATCY 1 cut(s) 437
BstZI CGGCCG 3 cut(s) 106, 110, 561
BsuRI GGCC 5 cut(s) 83, 108, 112, 404, 563
BtgZI GCGATG 1 cut(s) 873
BtsCI GGATG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 394
BtuMI TCGCGA 1 cut(s) 626
Cac8I GCNNGC 5 cut(s) 37, 70, 81, 110, 736
CfoI GCGC 4 cut(s) 41, 225, 736, 738
Cfr10I RCCGGY 2 cut(s) 108, 592
Cfr13I GGNCC 2 cut(s) 432, 840
CpoI CGGWCCG 1 cut(s) 432
CseI GACGC 5 cut(s) 85, 95, 191, 464, 521
Csp6I GTAC 1 cut(s) 190
CspAI ACCGGT 1 cut(s) 592
CspI CGGWCCG 1 cut(s) 432
CviAII CATG 1 cut(s) 814
CviQI GTAC 1 cut(s) 190
DpnI GATC 6 cut(s) 57, 338, 439, 467, 511, 522
DpnII GATC 6 cut(s) 55, 336, 437, 465, 509, 520
DraIII CACNNNGTG 1 cut(s) 784
EaeI YGGCCR 3 cut(s) 106, 110, 561
EagI CGGCCG 3 cut(s) 106, 110, 561
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 2 cut(s) 132, 669
EclXI CGGCCG 3 cut(s) 106, 110, 561
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 432, 840
Eco52I CGGCCG 3 cut(s) 106, 110, 561
EcoRI GAATTC 2 cut(s) 265, 289
Esp3I CGTCTC 4 cut(s) 68, 483, 560, 598
FaeI CATG 1 cut(s) 817
FaiI YATR 3 cut(s) 497, 575, 815
FaqI GGGAC 1 cut(s) 677
FatI CATG 1 cut(s) 813
FauI CCCGC 1 cut(s) 162
FblI GTMKAC 2 cut(s) 725, 762
Fnu4HI GCNGC 7 cut(s) 302, 333, 356, 392, 715, 747, 750
FokI GGATG 1 cut(s) 131
FseI GGCCGGCC 1 cut(s) 112
Fsp4HI GCNGC 7 cut(s) 302, 333, 356, 392, 715, 747, 750
FspBI CTAG 1 cut(s) 26
GlaI GCGC 4 cut(s) 40, 224, 735, 737
GluI GCNGC 7 cut(s) 302, 333, 356, 392, 715, 747, 750
HaeIII GGCC 5 cut(s) 83, 108, 112, 404, 563
HgaI GACGC 5 cut(s) 85, 95, 191, 464, 521
HhaI GCGC 4 cut(s) 41, 225, 736, 738
Hin1I GRCGYC 3 cut(s) 87, 123, 569
Hin1II CATG 1 cut(s) 817
Hin6I GCGC 4 cut(s) 39, 223, 734, 736
HinP1I GCGC 4 cut(s) 39, 223, 734, 736
HincII GTYRAC 2 cut(s) 726, 763
HindII GTYRAC 2 cut(s) 726, 763
HinfI GANTC 6 cut(s) 161, 230, 271, 385, 544, 764
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 3 cut(s) 691, 726, 763
Hpy188III TCNNGA 4 cut(s) 175, 435, 541, 625
Hpy8I GTNNAC 3 cut(s) 691, 726, 763
Hpy99I CGWCG 7 cut(s) 89, 128, 446, 478, 537, 730, 875
HpyAV CCTTC 4 cut(s) 175, 223, 322, 478
HpyCH4III ACNGT 2 cut(s) 432, 558
HpyCH4IV ACGT 3 cut(s) 123, 569, 873
HpyCH4V TGCA 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 95, 352, 361, 789
HpySE526I ACGT 3 cut(s) 123, 569, 873
Hsp92I GRCGYC 3 cut(s) 87, 123, 569
Hsp92II CATG 1 cut(s) 817
HspAI GCGC 4 cut(s) 39, 223, 734, 736
Kpn2I TCCGGA 1 cut(s) 434
KroI GCCGGC 1 cut(s) 108
KroNI GCCGGC 1 cut(s) 110
Kzo9I GATC 6 cut(s) 55, 336, 437, 465, 509, 520
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 3 cut(s) 313, 726, 761
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 123, 569, 873
MaeIII GTNAC 1 cut(s) 647
MalI GATC 6 cut(s) 57, 338, 439, 467, 511, 522
MbiI CCGCTC 1 cut(s) 746
MboI GATC 6 cut(s) 55, 336, 437, 465, 509, 520
MboII GAAGA 4 cut(s) 394, 526, 538, 613
MflI RGATCY 1 cut(s) 437
MluCI AATT 3 cut(s) 265, 277, 289
MlyI GAGTC 2 cut(s) 224, 758
MmeI TCCRAC 3 cut(s) 273, 424, 815
MnlI CCTC 8 cut(s) 109, 121, 130, 408, 495, 510, 711, 831
MroI TCCGGA 1 cut(s) 434
MroNI GCCGGC 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 821
MseI TTAA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 304, 752
MspR9I CCNGG 3 cut(s) 662, 801, 844
MvnI CGCG 3 cut(s) 626, 736, 738
MwoI GCNNNNNNNGC 5 cut(s) 45, 95, 352, 361, 789
NaeI GCCGGC 1 cut(s) 110
NciI CCSGG 3 cut(s) 662, 801, 844
NdeII GATC 6 cut(s) 55, 336, 437, 465, 509, 520
NgoMIV GCCGGC 1 cut(s) 108
NlaIII CATG 1 cut(s) 817
NlaIV GGNNCC 2 cut(s) 141, 439
NmeAIII GCCGAG 1 cut(s) 589
NmuCI GTSAC 1 cut(s) 647
NruI TCGCGA 1 cut(s) 626
NspV TTCGAA 1 cut(s) 269
PauI GCGCGC 1 cut(s) 734
PcsI WCGNNNNNNNCGW 7 cut(s) 189, 198, 216, 332, 458, 529, 566
PdiI GCCGGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 821
PfeI GAWTC 4 cut(s) 161, 271, 385, 544
PflFI GACNNNGTC 1 cut(s) 758
PinAI ACCGGT 1 cut(s) 592
PkrI GCNGC 7 cut(s) 303, 334, 357, 393, 716, 748, 751
PleI GAGTC 2 cut(s) 224, 758
PpsI GAGTC 2 cut(s) 224, 758
PspN4I GGNNCC 2 cut(s) 141, 439
PspPI GGNCC 2 cut(s) 432, 840
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PsuI RGATCY 1 cut(s) 437
PsyI GACNNNGTC 1 cut(s) 758
PteI GCGCGC 1 cut(s) 734
RigI GGCCGGCC 1 cut(s) 112
RruI TCGCGA 1 cut(s) 626
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
Rsr2I CGGWCCG 1 cut(s) 432
RsrII CGGWCCG 1 cut(s) 432
SalI GTCGAC 2 cut(s) 724, 761
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 7 cut(s) 302, 333, 356, 392, 715, 747, 750
Sau3AI GATC 6 cut(s) 55, 336, 437, 465, 509, 520
Sau96I GGNCC 2 cut(s) 432, 840
SchI GAGTC 2 cut(s) 224, 758
ScrFI CCNGG 3 cut(s) 662, 801, 844
SetI ASST 6 cut(s) 126, 141, 489, 572, 842, 876
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 2 cut(s) 432, 840
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 3 cut(s) 265, 277, 289
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 660, 799, 842
TaaI ACNGT 2 cut(s) 432, 558
TaiI ACGT 3 cut(s) 126, 572, 876
TasI AATT 3 cut(s) 265, 277, 289
TauI GCSGC 4 cut(s) 335, 358, 394, 749
TfiI GAWTC 4 cut(s) 161, 271, 385, 544
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 1 cut(s) 401
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 3 cut(s) 301, 714, 749
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 2 cut(s) 329, 830
TspGWI ACGGA 2 cut(s) 430, 574
TspRI CASTG 1 cut(s) 401
Tth111I GACNNNGTC 1 cut(s) 758
VpaK11BI GGWCC 2 cut(s) 432, 840
XapI RAATTY 3 cut(s) 265, 277, 289
XmiI GTMKAC 2 cut(s) 725, 762
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 1 cut(s) 26
ZraI GACGTC 2 cut(s) 124, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.