MD14G1023600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014262
Physical Location & Seq
Reverse (-)
2120506 .. 2121378
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1023600.v1.1.491

Sequence Viewer

Length: 873 bp
ATGCCACAAGTCGATTTGGAAACCCTAGTTTCGGCGTGTGCGGGAGGCTCAATGGATCGGAAAATCGCCTGCGAGACGCAGGCTGACGCCGCCACTGCTGGACACGACCGGCCGGAAGAAGATGCCGATGAACCGCAAGTTGCGCCGGATTTACCGCCGGAATCCTTCTGGCTCTCGAAAGACGCAGAGTACGACTGGTTCGACCGCAACGCTTTCTACGAGCGCAAAGACTCCACCAAAGGAAACTCCAACGCGTCGCACTCGAATTCGAACTCACAGAGGTTTTCGAAGAACTTGAAGTCGAAGGCGGCGATCATCGGGCTTCCGAAGCCGCAGAAGCACAACTACGCCGACCAGAAGAACCGGCGGCACTCCAAGGCCGGAAACACGAGACTGTTCCCGAAACGGACTGGATCCGTCGGGAAATCGGACGCTCCGATGATCGAGCCATCGTCTCCTAAGGTCTCGTGTATGGGGAGGGTGAGATCGAAGAGGGATAGAAAGCGTCGGTTCAGGAATCGGCACGGATCGTCGGCCGAGACGTCCATGGAGAAGTCCGTTAAACCGGTGGCGGAGAGACGAAAACTCGGTTTCTTTGCGAGTTTTCGGGCGATTTTTAGGCACGGAGGACACCGGGAGAAATCGGCGAAATCGCCGTTCGCGGAAGACTCGCCGCCGAGAAACAGCAGCGTGATGTCGACGTCGAGAGCTCGGGACAGGGCGGCGGCGGACGATTTCGACGCGCTGTCTATCGACTCGACGCCGAGATACAGCGTGGAGAGCGAACCGCCTGGTTTGGGCGGCATGAAGAGGTTCGTTTCGGGTCGGAGGTCCGGGGCGTGGGCGGGCGAAGTCGGTGTCGACGTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

31.99

Weight (kDa)

9.81

Isoelectric Point (pI)

60.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014262)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36220 AT3G52710
fragaria_vesca FvH4_6g18850
malus_domestica MD12G1024700.v1.1 MD14G1023600.v1.1
prunus_persica Prupe.7G109200_v2.0.a1
pyrus_communis pycom12g02440 pycom14g02250
rosa_laevigata RLG00000023978
rosa_multiflora Rmu_sc0005561.1_g000001
rosa_roxburghii Rroxscaffold_6G00407720
rosa_rugosa Rorug03G0136100
rosa_samantha Rh3AG186500 Rh3BG215100 Rh3CG211800 Rh3DG211000
rosa_wichuraiana Rw3G017060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 545, 704
AccI GTMKAC 2 cut(s) 698, 861
AccII CGCG 3 cut(s) 254, 662, 743
AclWI GGATC 4 cut(s) 63, 408, 421, 535
AcoI YGGCCR 2 cut(s) 110, 534
AcsI RAATTY 1 cut(s) 265
AcyI GRCGYC 4 cut(s) 87, 542, 701, 761
AfaI GTAC 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 31, 797, 840
AflIII ACRYGT 1 cut(s) 252
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 1 cut(s) 298
AhdI GACNNNNNGTC 1 cut(s) 745
AjnI CCWGG 1 cut(s) 790
AluBI AGCT 1 cut(s) 710
AluI AGCT 1 cut(s) 710
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 6 cut(s) 68, 385, 459, 469, 533, 571
AlwI GGATC 4 cut(s) 63, 408, 421, 535
Ama87I CYCGRG 1 cut(s) 711
AoxI GGCC 3 cut(s) 110, 378, 534
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 1 cut(s) 265
ArsI GACNNNNNNTTYG 1 cut(s) 843
AsiGI ACCGGT 1 cut(s) 565
Asp700I GAANNNNTTC 1 cut(s) 812
AspLEI GCGC 3 cut(s) 145, 225, 745
AspS9I GGNCC 1 cut(s) 831
AsuC2I CCSGG 2 cut(s) 635, 835
AsuHPI GGTGA 1 cut(s) 493
AsuII TTCGAA 2 cut(s) 269, 287
AvaI CYCGRG 1 cut(s) 711
AvaII GGWCC 1 cut(s) 831
AxyI CCTNAGG 1 cut(s) 459
BamHI GGATCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 712
BarI GAAGNNNNNNTAC 2 cut(s) 329, 361
BauI CACGAG 2 cut(s) 388, 466
BbsI GAAGAC 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 1 cut(s) 699
BccI CCATC 1 cut(s) 457
BceAI ACGGC 1 cut(s) 640
BciT130I CCWGG 1 cut(s) 792
BcnI CCSGG 2 cut(s) 635, 835
BcoDI GTCTC 6 cut(s) 68, 385, 459, 469, 533, 571
BfaI CTAG 1 cut(s) 26
BisI GCNGC 9 cut(s) 90, 309, 332, 368, 674, 688, 723, 726, 802
BlsI GCNGC 9 cut(s) 91, 310, 333, 369, 675, 689, 724, 727, 803
Bme1390I CCNGG 3 cut(s) 635, 792, 835
Bme18I GGWCC 1 cut(s) 831
BmeRI GACNNNNNGTC 1 cut(s) 745
BmeT110I CYCGRG 1 cut(s) 711
BmgT120I GGNCC 1 cut(s) 831
BmiI GGNNCC 1 cut(s) 415
BmrFI CCNGG 3 cut(s) 635, 792, 835
BmsI GCATC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 672
Bpu14I TTCGAA 2 cut(s) 269, 287
BpuMI CCSGG 2 cut(s) 635, 835
BsaHI GRCGYC 4 cut(s) 87, 542, 701, 761
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 3 cut(s) 375, 546, 834
BsaWI WCCGGW 1 cut(s) 565
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 797, 840
Bse118I RCCGGY 3 cut(s) 108, 363, 565
Bse1I ACTGG 2 cut(s) 200, 415
Bse21I CCTNAGG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 792
BseDI CCNNGG 3 cut(s) 375, 546, 834
BseLI CCNNNNNNNGG 3 cut(s) 31, 797, 840
BseNI ACTGG 2 cut(s) 200, 415
BseX3I CGGCCG 2 cut(s) 110, 534
BseXI GCAGC 1 cut(s) 699
Bsh1236I CGCG 3 cut(s) 254, 662, 743
Bsh1285I CGRYCG 4 cut(s) 109, 113, 205, 537
BshFI GGCC 3 cut(s) 112, 380, 536
BshTI ACCGGT 1 cut(s) 565
BsiEI CGRYCG 4 cut(s) 109, 113, 205, 537
BsiHKAI GWGCWC 1 cut(s) 712
BsiHKCI CYCGRG 1 cut(s) 711
BsiSI CCGG 9 cut(s) 109, 113, 146, 158, 364, 381, 566, 634, 834
BslFI GGGAC 1 cut(s) 728
BslI CCNNNNNNNGG 3 cut(s) 31, 797, 840
BsmAI GTCTC 6 cut(s) 68, 385, 459, 469, 533, 571
BsmBI CGTCTC 4 cut(s) 68, 459, 533, 571
BsmFI GGGAC 1 cut(s) 728
BsnI GGCC 3 cut(s) 112, 380, 536
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 711
Bsp119I TTCGAA 2 cut(s) 269, 287
Bsp1286I GDGCHC 1 cut(s) 712
Bsp143I GATC 6 cut(s) 55, 312, 413, 441, 485, 527
Bsp19I CCATGG 1 cut(s) 546
BspANI GGCC 3 cut(s) 112, 380, 536
BspFNI CGCG 3 cut(s) 254, 662, 743
BspLI GGNNCC 1 cut(s) 415
BspPI GGATC 4 cut(s) 63, 408, 421, 535
BspT104I TTCGAA 2 cut(s) 269, 287
BspTNI GGTCTC 1 cut(s) 469
BsrFI RCCGGY 3 cut(s) 108, 363, 565
BsrI ACTGG 2 cut(s) 200, 415
BssAI RCCGGY 3 cut(s) 108, 363, 565
BssECI CCNNGG 3 cut(s) 375, 546, 834
BssMI GATC 6 cut(s) 55, 312, 413, 441, 485, 527
BssNI GRCGYC 4 cut(s) 87, 542, 701, 761
BssSI CACGAG 2 cut(s) 388, 466
BssT1I CCWWGG 2 cut(s) 375, 546
Bst2BI CACGAG 2 cut(s) 388, 466
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 1 cut(s) 396
Bst6I CTCTTC 2 cut(s) 485, 803
BstACI GRCGYC 4 cut(s) 87, 542, 701, 761
BstBI TTCGAA 2 cut(s) 269, 287
BstC8I GCNNGC 3 cut(s) 70, 81, 847
BstDEI CTNAG 1 cut(s) 459
BstDSI CCRYGG 1 cut(s) 546
BstFNI CGCG 3 cut(s) 254, 662, 743
BstHHI GCGC 3 cut(s) 145, 225, 745
BstKTI GATC 6 cut(s) 58, 315, 416, 444, 488, 530
BstMAI GTCTC 6 cut(s) 68, 385, 459, 469, 533, 571
BstMBI GATC 6 cut(s) 55, 312, 413, 441, 485, 527
BstMCI CGRYCG 4 cut(s) 109, 113, 205, 537
BstMWI GCNNNNNNNGC 6 cut(s) 89, 95, 142, 328, 337, 780
BstNI CCWGG 1 cut(s) 792
BstSCI CCNGG 3 cut(s) 633, 790, 833
BstUI CGCG 3 cut(s) 254, 662, 743
BstV1I GCAGC 1 cut(s) 699
BstV2I GAAGAC 1 cut(s) 672
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BstZI CGGCCG 2 cut(s) 110, 534
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 3 cut(s) 112, 380, 536
BtgI CCRYGG 1 cut(s) 546
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 3 cut(s) 70, 81, 847
CfoI GCGC 3 cut(s) 145, 225, 745
Cfr10I RCCGGY 3 cut(s) 108, 363, 565
Cfr13I GGNCC 1 cut(s) 831
CseI GACGC 8 cut(s) 85, 95, 191, 243, 440, 494, 749, 769
Csp6I GTAC 1 cut(s) 190
CspAI ACCGGT 1 cut(s) 565
CviAII CATG 2 cut(s) 547, 805
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 1 cut(s) 459
DpnI GATC 6 cut(s) 57, 314, 415, 443, 487, 529
DpnII GATC 6 cut(s) 55, 312, 413, 441, 485, 527
DriI GACNNNNNGTC 1 cut(s) 745
EaeI YGGCCR 2 cut(s) 110, 534
EagI CGGCCG 2 cut(s) 110, 534
Eam1104I CTCTTC 2 cut(s) 485, 803
Eam1105I GACNNNNNGTC 1 cut(s) 745
EarI CTCTTC 2 cut(s) 485, 803
EciI GGCGGA 2 cut(s) 587, 743
Ecl136II GAGCTC 1 cut(s) 710
EclXI CGGCCG 2 cut(s) 110, 534
Eco130I CCWWGG 2 cut(s) 375, 546
Eco24I GRGCYC 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 831
Eco52I CGGCCG 2 cut(s) 110, 534
Eco53kI GAGCTC 1 cut(s) 710
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 711
EcoICRI GAGCTC 1 cut(s) 710
EcoRI GAATTC 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 790
EcoT14I CCWWGG 2 cut(s) 375, 546
EcoT38I GRGCYC 1 cut(s) 712
ErhI CCWWGG 2 cut(s) 375, 546
Esp3I CGTCTC 4 cut(s) 68, 459, 533, 571
FaeI CATG 2 cut(s) 550, 808
FaiI YATR 3 cut(s) 473, 548, 806
FaqI GGGAC 1 cut(s) 728
FatI CATG 2 cut(s) 546, 804
FauI CCCGC 2 cut(s) 34, 838
FblI GTMKAC 2 cut(s) 698, 861
Fnu4HI GCNGC 9 cut(s) 90, 309, 332, 368, 674, 688, 723, 726, 802
FriOI GRGCYC 1 cut(s) 712
Fsp4HI GCNGC 9 cut(s) 90, 309, 332, 368, 674, 688, 723, 726, 802
FspBI CTAG 1 cut(s) 26
GlaI GCGC 3 cut(s) 144, 224, 744
GluI GCNGC 9 cut(s) 90, 309, 332, 368, 674, 688, 723, 726, 802
HaeIII GGCC 3 cut(s) 112, 380, 536
HapII CCGG 9 cut(s) 109, 113, 146, 158, 364, 381, 566, 634, 834
HgaI GACGC 8 cut(s) 85, 95, 191, 243, 440, 494, 749, 769
HhaI GCGC 3 cut(s) 145, 225, 745
Hin1I GRCGYC 4 cut(s) 87, 542, 701, 761
Hin1II CATG 2 cut(s) 550, 808
Hin6I GCGC 3 cut(s) 143, 223, 743
HinP1I GCGC 3 cut(s) 143, 223, 743
HincII GTYRAC 2 cut(s) 699, 862
HindII GTYRAC 2 cut(s) 699, 862
HinfI GANTC 5 cut(s) 161, 230, 517, 668, 755
HpaII CCGG 9 cut(s) 109, 113, 146, 158, 364, 381, 566, 634, 834
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 699, 862
Hpy188I TCNGA 5 cut(s) 60, 327, 430, 438, 828
Hpy188III TCNNGA 6 cut(s) 175, 400, 421, 514, 705, 713
Hpy8I GTNNAC 2 cut(s) 699, 862
Hpy99I CGWCG 9 cut(s) 259, 422, 510, 535, 703, 706, 743, 763, 866
HpyAV CCTTC 2 cut(s) 175, 298
HpyCH4III ACNGT 1 cut(s) 396
HpyCH4IV ACGT 3 cut(s) 542, 701, 864
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 95, 142, 328, 337, 780
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 3 cut(s) 542, 701, 864
Hsp92I GRCGYC 4 cut(s) 87, 542, 701, 761
Hsp92II CATG 2 cut(s) 550, 808
HspAI GCGC 3 cut(s) 143, 223, 743
Kzo9I GATC 6 cut(s) 55, 312, 413, 441, 485, 527
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 1 cut(s) 699
LweI GCATC 1 cut(s) 112
MaeI CTAG 1 cut(s) 26
MaeII ACGT 3 cut(s) 542, 701, 864
MalI GATC 6 cut(s) 57, 314, 415, 443, 487, 529
MboI GATC 6 cut(s) 55, 312, 413, 441, 485, 527
MboII GAAGA 7 cut(s) 128, 131, 301, 370, 502, 677, 820
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 712
MluCI AATT 1 cut(s) 265
MluI ACGCGT 1 cut(s) 252
MlyI GAGTC 3 cut(s) 224, 662, 749
MmeI TCCRAC 2 cut(s) 273, 806
MnlI CCTC 7 cut(s) 38, 273, 471, 486, 620, 804, 822
MroXI GAANNNNTTC 1 cut(s) 812
MseI TTAA 1 cut(s) 561
MspI CCGG 9 cut(s) 109, 113, 146, 158, 364, 381, 566, 634, 834
MspR9I CCNGG 3 cut(s) 635, 792, 835
MvaI CCWGG 1 cut(s) 792
MvnI CGCG 3 cut(s) 254, 662, 743
MwoI GCNNNNNNNGC 6 cut(s) 89, 95, 142, 328, 337, 780
NciI CCSGG 2 cut(s) 635, 835
NcoI CCATGG 1 cut(s) 546
NdeII GATC 6 cut(s) 55, 312, 413, 441, 485, 527
NlaIII CATG 2 cut(s) 550, 808
NlaIV GGNNCC 1 cut(s) 415
NmeAIII GCCGAG 3 cut(s) 562, 702, 789
NspV TTCGAA 2 cut(s) 269, 287
PcsI WCGNNNNNNNCGW 7 cut(s) 189, 198, 216, 308, 434, 539, 861
PdmI GAANNNNTTC 1 cut(s) 812
PfeI GAWTC 2 cut(s) 161, 517
PinAI ACCGGT 1 cut(s) 565
PkrI GCNGC 9 cut(s) 91, 310, 333, 369, 675, 689, 724, 727, 803
PleI GAGTC 3 cut(s) 224, 662, 749
PpsI GAGTC 3 cut(s) 224, 662, 749
Psp124BI GAGCTC 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 790
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 1 cut(s) 415
PspPI GGNCC 1 cut(s) 831
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SacI GAGCTC 1 cut(s) 712
SalI GTCGAC 2 cut(s) 697, 860
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 9 cut(s) 90, 309, 332, 368, 674, 688, 723, 726, 802
Sau3AI GATC 6 cut(s) 55, 312, 413, 441, 485, 527
Sau96I GGNCC 1 cut(s) 831
SchI GAGTC 3 cut(s) 224, 662, 749
ScrFI CCNGG 3 cut(s) 635, 792, 835
SduI GDGCHC 1 cut(s) 712
SetI ASST 8 cut(s) 284, 465, 545, 704, 712, 815, 833, 867
SfaNI GCATC 1 cut(s) 112
SfuI TTCGAA 2 cut(s) 269, 287
SinI GGWCC 1 cut(s) 831
Sse9I AATT 1 cut(s) 265
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 712
StyD4I CCNGG 3 cut(s) 633, 790, 833
StyI CCWWGG 2 cut(s) 375, 546
TaaI ACNGT 1 cut(s) 396
TaiI ACGT 3 cut(s) 545, 704, 867
TasI AATT 1 cut(s) 265
TauI GCSGC 8 cut(s) 92, 311, 334, 370, 676, 725, 728, 804
TfiI GAWTC 2 cut(s) 161, 517
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 1 cut(s) 100
TseI GCWGC 1 cut(s) 687
TspDTI ATGAA 2 cut(s) 144, 821
TspGWI ACGGA 5 cut(s) 406, 421, 540, 547, 639
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 831
XapI RAATTY 1 cut(s) 265
XmiI GTMKAC 2 cut(s) 698, 861
XmnI GAANNNNTTC 1 cut(s) 812
XspI CTAG 1 cut(s) 26
ZraI GACGTC 2 cut(s) 543, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.