MD12G1025100.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013403
Physical Location & Seq
Forward (+)
2690133 .. 2692975
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1025100.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGGCGGAAGAAAGAGGAAGCTGAAATCGTTAGCTGAAACAATCACGAACAGTAACAGAAAAGCAAGCAAGAAAAGGGAGAAATTGGAGCTGCAAACTTGGTCTTACCTTCCTGCTGAACTTTTGGAACTCATTGTGTCTCGTTTAACCCTGCGGGATAATATTCGTGTATCTGTTGTGTGCAAGAGATGGCACTCAGCTGCCATTTCGGTGCGGGTGGTCAACCAATCACCATTGCTTATGTACTTTCCCAGATTTGGTGACCTGTATGAATTCTATGATCCATCGCAGCGTAAAATCTACTCCTTGGAGTTACCTGAGCTGACTGGGTCTAGGATTTGCTACACTAAAGACGTTGTTGCTATTAGCACTTGTCGTCCTGGGGAAAGAGAGTGGACTACTGTTAATTTCCAAAACCGCTTGCCCTTTGTGAGTAGCATCTGGAACAAGCTTGCTTTCTGCAACGGAGTCTTTTATTGTCTGAGCCTCACCGGTTGGTTAGGGGTATTCAACCCCGAAGAACGCAGTTGGAGTGTTCTTGCTGTTCCTCCACCCAAGTGCCCAGAGAACTTTTTTGCCAAAAATTGGTGGAAAGGTAAGTTTATGGCAGAGCATGAGGGAAACATACTAGTTATATATACTTGTTCAAGTGAAAACCCAATTATATTTAAGCTGGATCAGACAAACATGGTTTGGGAAGAAATGACGACCCTCGATGGTGTTACACTCTTTGCGAGTTTCTTGTCGTCTCATGCAAGAACCGACCTCCCTGGAATAATGAGAAACAGTGTCTACTTCTCGAAAGTCCGCTTCTACGGAAGGTGTTGCATATCCTACTCTCTCGACTCTTGTAGATACCACCCTCGCAAGGATTGCCATGACTGGGGAGAGCAAGATCCTTTTAAGAAGATTTGGATTGAAGCACCCCAAGACTTCTCAACCTTAAATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

318

Amino Acids

36.96

Weight (kDa)

9.05

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 34 - 68 4e-08 F-box-like
F-box PF00646 34 - 72 9.8e-08 F-box domain
Beta-prop_KIB1-4 PF03478 119 - 268 9.9e-16 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57790
fragaria_vesca FvH4_6g18890
malus_domestica MD12G1025100.v1.1 MD14G1023800.v1.1
prunus_persica Prupe.7G109000_v2.0.a1
pyrus_communis pycom12g02460 pycom14g02290
rosa_chinensis RchiOBHm_Chr3g0473881
rosa_laevigata RLG00000023976
rosa_multiflora Rmu_sc0002414.1_g000044
rosa_roxburghii Rroxscaffold_6G00407700
rosa_rugosa Rorug03G0136200 Rorug03G0136300
rosa_samantha Rh3AG186800 Rh3BG215400 Rh3CG212100 Rh3DG211300
rosa_wichuraiana Rw3G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 585
AccI GTMKAC 1 cut(s) 792
AciI CCGC 5 cut(s) 6, 154, 214, 418, 808
AclWI GGATC 3 cut(s) 275, 684, 890
AcsI RAATTY 1 cut(s) 272
AfaI GTAC 1 cut(s) 245
AfiI CCNNNNNNNGG 5 cut(s) 257, 585, 868, 883, 948
AgeI ACCGGT 1 cut(s) 491
AgsI TTSAA 3 cut(s) 511, 648, 920
AhlI ACTAGT 1 cut(s) 628
AjnI CCWGG 2 cut(s) 379, 769
AleI CACNNNNGTG 1 cut(s) 556
AluBI AGCT 7 cut(s) 22, 35, 91, 200, 322, 451, 673
AluI AGCT 7 cut(s) 22, 35, 91, 200, 322, 451, 673
Alw26I GTCTC 2 cut(s) 144, 753
AlwI GGATC 3 cut(s) 275, 684, 890
ApeKI GCWGC 3 cut(s) 91, 200, 289
ApoI RAATTY 1 cut(s) 272
AsiGI ACCGGT 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 222, 272, 481
BaeGI GKGCMC 1 cut(s) 563
BbvI GCAGC 3 cut(s) 78, 187, 301
BccI CCATC 3 cut(s) 183, 292, 710
BciT130I CCWGG 2 cut(s) 381, 771
BcoDI GTCTC 2 cut(s) 144, 753
BcuI ACTAGT 1 cut(s) 628
BfaI CTAG 2 cut(s) 333, 629
BisI GCNGC 3 cut(s) 92, 201, 290
BlsI GCNGC 3 cut(s) 93, 202, 291
Bme1390I CCNGG 2 cut(s) 381, 771
BmrFI CCNGG 2 cut(s) 381, 771
BmrI ACTGGG 2 cut(s) 336, 892
BmsI GCATC 1 cut(s) 447
BmuI ACTGGG 2 cut(s) 336, 892
Bpu10I CCTNAGC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 306, 380, 769
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 585, 868, 883, 948
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 2 cut(s) 331, 887
Bse3DI GCAATG 1 cut(s) 233
BseBI CCWGG 2 cut(s) 381, 771
BseDI CCNNGG 3 cut(s) 306, 380, 769
BseLI CCNNNNNNNGG 5 cut(s) 257, 585, 868, 883, 948
BseMI GCAATG 1 cut(s) 233
BseMII CTCAG 3 cut(s) 210, 309, 473
BseNI ACTGG 2 cut(s) 331, 887
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 3 cut(s) 78, 187, 301
BshTI ACCGGT 1 cut(s) 491
BsiSI CCGG 1 cut(s) 492
BslI CCNNNNNNNGG 5 cut(s) 257, 585, 868, 883, 948
BsmAI GTCTC 2 cut(s) 144, 753
BsmBI CGTCTC 1 cut(s) 753
Bsp1286I GDGCHC 1 cut(s) 563
Bsp143I GATC 3 cut(s) 280, 676, 895
BspACI CCGC 5 cut(s) 6, 154, 214, 418, 808
BspCNI CTCAG 3 cut(s) 209, 310, 474
BspPI GGATC 3 cut(s) 275, 684, 890
BsrDI GCAATG 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 491
BsrI ACTGG 2 cut(s) 331, 887
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 306, 380, 769
BssMI GATC 3 cut(s) 280, 676, 895
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 381, 771
Bst4CI ACNGT 3 cut(s) 53, 403, 788
BstAPI GCANNNNNTGC 1 cut(s) 873
BstC8I GCNNGC 3 cut(s) 67, 422, 453
BstDEI CTNAG 3 cut(s) 196, 318, 482
BstEII GGTNACC 1 cut(s) 260
BstKTI GATC 3 cut(s) 283, 679, 898
BstMAI GTCTC 2 cut(s) 144, 753
BstMBI GATC 3 cut(s) 280, 676, 895
BstMWI GCNNNNNNNGC 1 cut(s) 873
BstNI CCWGG 2 cut(s) 381, 771
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 379, 769
BstSLI GKGCMC 1 cut(s) 563
BstV1I GCAGC 3 cut(s) 78, 187, 301
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BtgZI GCGATG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 793
Cac8I GCNNGC 3 cut(s) 67, 422, 453
Cfr10I RCCGGY 1 cut(s) 491
Csp6I GTAC 1 cut(s) 244
CspAI ACCGGT 1 cut(s) 491
CviAII CATG 4 cut(s) 614, 688, 752, 878
CviJI RGCY 8 cut(s) 22, 35, 91, 200, 322, 451, 486, 673
CviKI_1 RGCY 8 cut(s) 22, 35, 91, 200, 322, 451, 486, 673
CviQI GTAC 1 cut(s) 244
DdeI CTNAG 3 cut(s) 196, 318, 482
DpnI GATC 3 cut(s) 282, 678, 897
DpnII GATC 3 cut(s) 280, 676, 895
EciI GGCGGA 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 379, 769
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 753
FaeI CATG 4 cut(s) 617, 691, 755, 881
FatI CATG 4 cut(s) 613, 687, 751, 877
FauI CCCGC 2 cut(s) 147, 207
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 3 cut(s) 92, 201, 290
Fsp4HI GCNGC 3 cut(s) 92, 201, 290
FspBI CTAG 2 cut(s) 333, 629
GluI GCNGC 3 cut(s) 92, 201, 290
HapII CCGG 1 cut(s) 492
Hin1II CATG 4 cut(s) 617, 691, 755, 881
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 2 cut(s) 468, 845
HpaII CCGG 1 cut(s) 492
HphI GGTGA 3 cut(s) 222, 272, 481
Hpy166II GTNNAC 3 cut(s) 223, 396, 793
Hpy188I TCNGA 2 cut(s) 483, 681
Hpy188III TCNNGA 4 cut(s) 46, 442, 799, 842
Hpy8I GTNNAC 3 cut(s) 223, 396, 793
HpyAV CCTTC 2 cut(s) 119, 813
HpyCH4III ACNGT 3 cut(s) 53, 403, 788
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 5 cut(s) 94, 183, 462, 755, 828
HpyF10VI GCNNNNNNNGC 1 cut(s) 873
HpyF3I CTNAG 3 cut(s) 196, 318, 482
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 4 cut(s) 617, 691, 755, 881
Kzo9I GATC 3 cut(s) 280, 676, 895
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 3 cut(s) 78, 187, 301
LweI GCATC 1 cut(s) 447
MaeI CTAG 2 cut(s) 333, 629
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 4 cut(s) 53, 260, 312, 721
MalI GATC 3 cut(s) 282, 678, 897
MboI GATC 3 cut(s) 280, 676, 895
MboII GAAGA 4 cut(s) 21, 530, 710, 919
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 1 cut(s) 563
MluCI AATT 6 cut(s) 83, 272, 406, 583, 660, 946
MlyI GAGTC 2 cut(s) 477, 839
MmeI TCCRAC 1 cut(s) 509
MnlI CCTC 7 cut(s) 9, 497, 558, 610, 722, 776, 873
MseI TTAA 5 cut(s) 146, 405, 669, 903, 944
MslI CAYNNNNRTG 2 cut(s) 209, 556
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 2 cut(s) 381, 771
MvaI CCWGG 2 cut(s) 381, 771
MwoI GCNNNNNNNGC 1 cut(s) 873
NdeII GATC 3 cut(s) 280, 676, 895
NlaIII CATG 4 cut(s) 617, 691, 755, 881
NmuCI GTSAC 1 cut(s) 260
OliI CACNNNNGTG 1 cut(s) 556
PflFI GACNNNGTC 1 cut(s) 328
PflMI CCANNNNNTGG 1 cut(s) 585
PinAI ACCGGT 1 cut(s) 491
PkrI GCNGC 3 cut(s) 93, 202, 291
PleI GAGTC 2 cut(s) 476, 839
PpsI GAGTC 2 cut(s) 476, 839
Psp6I CCWGG 2 cut(s) 379, 769
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 2 cut(s) 379, 769
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 2 cut(s) 209, 556
SaqAI TTAA 5 cut(s) 146, 405, 669, 903, 944
SatI GCNGC 3 cut(s) 92, 201, 290
Sau3AI GATC 3 cut(s) 280, 676, 895
SchI GAGTC 2 cut(s) 477, 839
ScrFI CCNGG 2 cut(s) 381, 771
SduI GDGCHC 1 cut(s) 563
SfaNI GCATC 1 cut(s) 447
SmiMI CAYNNNNRTG 2 cut(s) 209, 556
SpeI ACTAGT 1 cut(s) 628
Sse9I AATT 6 cut(s) 83, 272, 406, 583, 660, 946
SsiI CCGC 5 cut(s) 6, 154, 214, 418, 808
SspI AATATT 1 cut(s) 163
SspMI CTAG 2 cut(s) 333, 629
StyD4I CCNGG 2 cut(s) 379, 769
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 3 cut(s) 53, 403, 788
TaiI ACGT 1 cut(s) 357
TaqI TCGA 3 cut(s) 714, 800, 843
TasI AATT 6 cut(s) 83, 272, 406, 583, 660, 946
TatI WGTACW 1 cut(s) 243
Tru1I TTAA 5 cut(s) 146, 405, 669, 903, 944
Tru9I TTAA 5 cut(s) 146, 405, 669, 903, 944
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 3 cut(s) 91, 200, 289
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 2 cut(s) 480, 831
TspRI CASTG 1 cut(s) 793
Tth111I GACNNNGTC 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 585
XapI RAATTY 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 2 cut(s) 333, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.