pycom12g02460

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013403
Physical Location & Seq
Forward (+)
2151261 .. 2153848
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02460.2

Sequence Viewer

Length: 1131 bp
ATGGGCGGAAGAAAGAGGAAGCTGAAATCGTTAGCTGAAACAATCACAAACAGTAACAGAAAAGCAAGCAAGAAAAAGGAGAAATTGGAGCTGCAAACTTGGTCTGACCTTCCTGCTGAACTTTTGGAACTCATTGTGTCTCGTTTAACGCTGCGGGATAATATTCGTGTATCTGTTGTGTGCAAGAGATGGCACTCAGCTGCCATTTCTGTGCGGGTGATCAACCAATCACCATTGCTTATGTACTTTCCCAGATTTGGTGATCTATATGAATTCTATGATCCATCGCGGCGCAAAATCTACTCCTTGGAGTTACCTGAGCTGACTGGGTCTAGGATTTGCTACACTAAAGATGGTTGGCTACTGTTATACAGACCCAGATCTCACCGTGTGTTCTTCTTTAATCCCTTCACTCGCAAGATAATAAAGGTACCAAGATTCGAGTTGACTTATCAGATAGTTGCATTCTCCTGTGCCCCAACTTCTTCCAGCTGCATACTGTTTACGTTGAAACACATCAGCCCCACGGTTGTTGCTATTAGCACTTGTCGTCCTGGGGAAAGAGAGTGGACTACTGTTAATCTCCAAAACCGCTTGCCCTTTGTGAGTAGCATCTGGAACAAGCTTGTTTTCTGCAACGGAGTCTTTTATTGTCTGAGCCTCACCGGTTGGTTAGGGGTGTTCAACCCCGAAGAACGCAGTTGGAGTGTTCTTGCTGTTCCTCCACCCAAGTGCCCAGAGAACTTTTTTGCCAAAAATTGGTGGAAAGGTAAGTTTATGGCAGAGCATGAGGGAAACATATTAGTTATATATACTTGTTCAAGTGAAAACCCAATTATATTTAAGCTGGATCAGACAAACATGGTTTGGGAAGAAATGACGACCCTCGATGGTGTGACACTCTTTGCGAGTTTCTTGTCGTCTCATGCAAGAACTGACCTCCCTGGAATAATGAGAAACAGTGTCTACTTCTCGAAAGTCCGTTTCTACGGAAGGTGTTGCATATCCTACTCTCTCGACTCTTGTAGATACCACCCTCGCAAGGATTGCCATGACTGGGGAGAGCAAGATCGATCTTTTAAGAAGATTTGGATTGAAGCACCTCAAGACTTCTCAACCTTAAATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

377

Amino Acids

43.88

Weight (kDa)

9.41

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57790
fragaria_vesca FvH4_6g18890
malus_domestica MD12G1025100.v1.1 MD14G1023800.v1.1
prunus_persica Prupe.7G109000_v2.0.a1
pyrus_communis pycom12g02460 pycom14g02290
rosa_chinensis RchiOBHm_Chr3g0473881
rosa_laevigata RLG00000023976
rosa_multiflora Rmu_sc0002414.1_g000044
rosa_roxburghii Rroxscaffold_6G00407700
rosa_rugosa Rorug03G0136200 Rorug03G0136300
rosa_samantha Rh3AG186800 Rh3BG215400 Rh3CG212100 Rh3DG211300
rosa_wichuraiana Rw3G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 430
AccB1I GGYRCC 1 cut(s) 430
AccB7I CCANNNNNTGG 1 cut(s) 759
AccI GTMKAC 1 cut(s) 966
AccII CGCG 1 cut(s) 289
AciI CCGC 5 cut(s) 6, 154, 214, 289, 592
AclWI GGATC 2 cut(s) 275, 858
AcsI RAATTY 1 cut(s) 272
AdeI CACNNNGTG 1 cut(s) 391
AfaI GTAC 2 cut(s) 245, 432
AfiI CCNNNNNNNGG 5 cut(s) 257, 759, 1042, 1057, 1125
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 4 cut(s) 511, 685, 822, 1097
AjnI CCWGG 2 cut(s) 553, 943
AleI CACNNNNGTG 1 cut(s) 730
AluBI AGCT 8 cut(s) 22, 35, 91, 200, 322, 492, 625, 847
AluI AGCT 8 cut(s) 22, 35, 91, 200, 322, 492, 625, 847
Alw26I GTCTC 2 cut(s) 144, 927
AlwI GGATC 2 cut(s) 275, 858
ApeKI GCWGC 4 cut(s) 91, 151, 200, 492
ApoI RAATTY 1 cut(s) 272
AsiGI ACCGGT 1 cut(s) 665
Asp718I GGTACC 1 cut(s) 430
AspLEI GCGC 1 cut(s) 294
AsuHPI GGTGA 5 cut(s) 222, 229, 272, 377, 655
BaeGI GKGCMC 2 cut(s) 478, 737
BanI GGYRCC 1 cut(s) 430
BbvI GCAGC 4 cut(s) 78, 138, 187, 479
BccI CCATC 4 cut(s) 183, 292, 347, 884
BciT130I CCWGG 2 cut(s) 555, 945
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 144, 927
BfaI CTAG 1 cut(s) 333
BglII AGATCT 1 cut(s) 380
BisI GCNGC 5 cut(s) 92, 152, 201, 290, 493
BlsI GCNGC 5 cut(s) 93, 153, 202, 291, 494
Bme1390I CCNGG 2 cut(s) 555, 945
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 2 cut(s) 555, 945
BmrI ACTGGG 2 cut(s) 336, 1066
BmsI GCATC 1 cut(s) 621
BmuI ACTGGG 2 cut(s) 336, 1066
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 1089
Bsa29I ATCGAT 1 cut(s) 1072
BsaJI CCNNGG 4 cut(s) 306, 525, 554, 943
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 759, 1042, 1057, 1125
Bse118I RCCGGY 1 cut(s) 665
Bse1I ACTGG 2 cut(s) 331, 1061
Bse3DI GCAATG 1 cut(s) 233
BseBI CCWGG 2 cut(s) 555, 945
BseCI ATCGAT 1 cut(s) 1072
BseDI CCNNGG 4 cut(s) 306, 525, 554, 943
BseLI CCNNNNNNNGG 5 cut(s) 257, 759, 1042, 1057, 1125
BseMI GCAATG 1 cut(s) 233
BseMII CTCAG 3 cut(s) 210, 309, 647
BseNI ACTGG 2 cut(s) 331, 1061
BseSI GKGCMC 2 cut(s) 478, 737
BseXI GCAGC 4 cut(s) 78, 138, 187, 479
Bsh1236I CGCG 1 cut(s) 289
BshNI GGYRCC 1 cut(s) 430
BshTI ACCGGT 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 1072
BsiSI CCGG 1 cut(s) 666
BslI CCNNNNNNNGG 5 cut(s) 257, 759, 1042, 1057, 1125
BsmAI GTCTC 2 cut(s) 144, 927
BsmBI CGTCTC 1 cut(s) 927
BsmI GAATGC 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 478, 737
Bsp143I GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
BspACI CCGC 5 cut(s) 6, 154, 214, 289, 592
BspCNI CTCAG 3 cut(s) 209, 310, 648
BspDI ATCGAT 1 cut(s) 1072
BspFNI CGCG 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 2 cut(s) 275, 858
BspT107I GGYRCC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 665
BsrI ACTGG 2 cut(s) 331, 1061
BssAI RCCGGY 1 cut(s) 665
BssECI CCNNGG 4 cut(s) 306, 525, 554, 943
BssMI GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 555, 945
Bst4CI ACNGT 7 cut(s) 53, 366, 389, 501, 529, 577, 962
BstAPI GCANNNNNTGC 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 67, 596
BstDEI CTNAG 3 cut(s) 196, 318, 656
BstDSI CCRYGG 1 cut(s) 525
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 7 cut(s) 222, 265, 283, 383, 853, 1072, 1076
BstMAI GTCTC 2 cut(s) 144, 927
BstMBI GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
BstMWI GCNNNNNNNGC 1 cut(s) 1047
BstNI CCWGG 2 cut(s) 555, 945
BstSCI CCNGG 2 cut(s) 553, 943
BstSLI GKGCMC 2 cut(s) 478, 737
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 4 cut(s) 78, 138, 187, 479
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
Bsu15I ATCGAT 1 cut(s) 1072
BsuTUI ATCGAT 1 cut(s) 1072
BtgI CCRYGG 1 cut(s) 525
BtgZI GCGATG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 967
Cac8I GCNNGC 2 cut(s) 67, 596
CfoI GCGC 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 1072
Csp6I GTAC 2 cut(s) 244, 431
CspAI ACCGGT 1 cut(s) 665
CspCI CAANNNNNGTGG 2 cut(s) 514, 549
CviAII CATG 4 cut(s) 788, 862, 926, 1052
CviQI GTAC 2 cut(s) 244, 431
DdeI CTNAG 3 cut(s) 196, 318, 656
DpnI GATC 7 cut(s) 221, 264, 282, 382, 852, 1071, 1075
DpnII GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
DraIII CACNNNGTG 1 cut(s) 391
EciI GGCGGA 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 553, 943
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 927
FaeI CATG 4 cut(s) 791, 865, 929, 1055
FatI CATG 4 cut(s) 787, 861, 925, 1051
FauI CCCGC 2 cut(s) 147, 207
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 5 cut(s) 92, 152, 201, 290, 493
Fsp4HI GCNGC 5 cut(s) 92, 152, 201, 290, 493
FspBI CTAG 1 cut(s) 333
GlaI GCGC 1 cut(s) 293
GluI GCNGC 5 cut(s) 92, 152, 201, 290, 493
HapII CCGG 1 cut(s) 666
HhaI GCGC 1 cut(s) 294
Hin1II CATG 4 cut(s) 791, 865, 929, 1055
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 3 cut(s) 438, 642, 1019
HpaII CCGG 1 cut(s) 666
HphI GGTGA 5 cut(s) 222, 229, 272, 377, 655
Hpy166II GTNNAC 4 cut(s) 447, 504, 570, 967
Hpy188I TCNGA 4 cut(s) 106, 456, 657, 855
Hpy188III TCNNGA 4 cut(s) 616, 973, 1016, 1106
Hpy8I GTNNAC 4 cut(s) 447, 504, 570, 967
HpyAV CCTTC 3 cut(s) 119, 418, 987
HpyCH4III ACNGT 7 cut(s) 53, 366, 389, 501, 529, 577, 962
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 7 cut(s) 94, 183, 464, 495, 636, 929, 1002
HpyF10VI GCNNNNNNNGC 1 cut(s) 1047
HpyF3I CTNAG 3 cut(s) 196, 318, 656
HpySE526I ACGT 1 cut(s) 506
Hsp92II CATG 4 cut(s) 791, 865, 929, 1055
HspAI GCGC 1 cut(s) 292
KpnI GGTACC 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 4 cut(s) 78, 138, 187, 479
LweI GCATC 1 cut(s) 621
MaeI CTAG 1 cut(s) 333
MaeII ACGT 1 cut(s) 506
MaeIII GTNAC 3 cut(s) 53, 312, 895
MalI GATC 7 cut(s) 221, 264, 282, 382, 852, 1071, 1075
MboI GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
MboII GAAGA 6 cut(s) 21, 388, 477, 704, 884, 1096
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 478, 737
MluCI AATT 5 cut(s) 83, 272, 757, 834, 1123
MlyI GAGTC 2 cut(s) 651, 1013
MmeI TCCRAC 1 cut(s) 683
MnlI CCTC 8 cut(s) 9, 671, 732, 784, 896, 950, 1047, 1113
MseI TTAA 6 cut(s) 146, 402, 579, 843, 1080, 1121
MslI CAYNNNNRTG 2 cut(s) 209, 730
MspA1I CMGCKG 2 cut(s) 200, 492
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 2 cut(s) 555, 945
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 555, 945
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 1 cut(s) 1047
NdeII GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
NlaIII CATG 4 cut(s) 791, 865, 929, 1055
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 895
OliI CACNNNNGTG 1 cut(s) 730
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 1 cut(s) 438
PflFI GACNNNGTC 1 cut(s) 328
PflMI CCANNNNNTGG 1 cut(s) 759
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 5 cut(s) 93, 153, 202, 291, 494
PleI GAGTC 2 cut(s) 650, 1013
PpsI GAGTC 2 cut(s) 650, 1013
Psp6I CCWGG 2 cut(s) 553, 943
PspGI CCWGG 2 cut(s) 553, 943
PspN4I GGNNCC 1 cut(s) 432
PsuI RGATCY 1 cut(s) 380
PsyI GACNNNGTC 1 cut(s) 328
PvuII CAGCTG 2 cut(s) 200, 492
RsaI GTAC 2 cut(s) 245, 432
RsaNI GTAC 2 cut(s) 244, 431
RseI CAYNNNNRTG 2 cut(s) 209, 730
SaqAI TTAA 6 cut(s) 146, 402, 579, 843, 1080, 1121
SatI GCNGC 5 cut(s) 92, 152, 201, 290, 493
Sau3AI GATC 7 cut(s) 219, 262, 280, 380, 850, 1069, 1073
SchI GAGTC 2 cut(s) 651, 1013
ScrFI CCNGG 2 cut(s) 555, 945
SduI GDGCHC 2 cut(s) 478, 737
SfaNI GCATC 1 cut(s) 621
SmiMI CAYNNNNRTG 2 cut(s) 209, 730
SmlI CTYRAG 1 cut(s) 1104
SmoI CTYRAG 1 cut(s) 1104
Sse9I AATT 5 cut(s) 83, 272, 757, 834, 1123
SsiI CCGC 5 cut(s) 6, 154, 214, 289, 592
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 553, 943
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 7 cut(s) 53, 366, 389, 501, 529, 577, 962
TaiI ACGT 1 cut(s) 509
TaqI TCGA 5 cut(s) 441, 888, 974, 1017, 1072
TasI AATT 5 cut(s) 83, 272, 757, 834, 1123
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 292
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 6 cut(s) 146, 402, 579, 843, 1080, 1121
Tru9I TTAA 6 cut(s) 146, 402, 579, 843, 1080, 1121
TscAI CASTG 1 cut(s) 967
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 4 cut(s) 91, 151, 200, 492
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 1 cut(s) 285
TspGWI ACGGA 3 cut(s) 654, 971, 1005
TspRI CASTG 1 cut(s) 967
Tth111I GACNNNGTC 1 cut(s) 328
Van91I CCANNNNNTGG 1 cut(s) 759
XapI RAATTY 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 966
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.