Rorug03G0136300

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013403
Physical Location & Seq
Forward (+)
11181681 .. 11211162
29482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0136300.1

Sequence Viewer

Length: 1098 bp
ATGGTTTCAAATCCTGATCATGTCACATTAGCAGAAGGGGAGGTTGCACCAAGTTTTGCCTCATCTCTGCCAGTACCAAATATTCAAGAAACAGTGAGAGATGATCCTTTGCTGGTCCCAGAAAGATACCTAAGGAATAAAGAAGACGTGCCAAAGATTGCAGATACATGTCATGATATTGCTTCTGAGATTCCTATCATTGATTTTTCTCTGCTTTTAAAACAGCACAAAGAGGAGCTTAACAAGCTGGACATGGCTTCCAAAGAATGGGGATTCTTTCAGGTAGTAAATCATGGAGTGGAAAAAGAAGTGTTGCAGGGCATGAAGGATGTTGCGGCCAAGTTTTTTGAGCTTCCTCTGGAAGAGAAGAATAAGGTTGCTATGCCTTCAGATGACATACAAGGCTACGGCCATGCCTATGTGGTTTCTGAAGACCAGATTCTGGATTGGTCTGATGCATTGATTCTCATCGTGTACCCAACTCATTATAGAAAGCTTCAGTTTTGGCCAGCAGTACCAGAGAAATTCAAGGAGGTTCTTGATACATATTCTAGTGAAGTTAAAAGAGTAGGAGAGGAGCTTCTAATGTCTCTATCGATACTCATGGGAATGGAGAAGGATTCTCTTATTGGCCTCCATAACCAGTTTTTACAAGGTGTGAGGGTGAACTACTATCCTCCATGCTCCATGCCTCATAAAGTACTAGGACTGAGTCCACACTCGGACACGAGCACTATAACCATACTTATGCAAGAGGACGATGTCACCGGTTTGCAGATTCAGAAAGGAGGAGAATGGGTGTCAGTCGAGCCAATTCCAAACGCTCTTGTTGTGAATGTTGGAGATGTTCTTGAGATATGGACTAATGGGAAGTACAAGAGCATTGAGCACAGAGCTGTGACGACCAAAAACAAGGCGAGGTTATCCTATGCAACATTTCTTTCCCCACATGACGATGTGGAAGTTGAACCAATTTATGATATTGTGGAGTCACATATGATGTACAAGAAAGTCAGATATGGAGATTATCTCAGACAGTCCATGAAGATGAAGCATGCGGGGAAGGCACACACTCAAATGGCGAAGATCGAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

365

Amino Acids

41.41

Weight (kDa)

5.34

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 64 - 171 2e-29 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 219 - 316 2.1e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57790
fragaria_vesca FvH4_6g18890
malus_domestica MD12G1025100.v1.1 MD14G1023800.v1.1
prunus_persica Prupe.7G109000_v2.0.a1
pyrus_communis pycom12g02460 pycom14g02290
rosa_chinensis RchiOBHm_Chr3g0473881
rosa_laevigata RLG00000023976
rosa_multiflora Rmu_sc0002414.1_g000044
rosa_roxburghii Rroxscaffold_6G00407700
rosa_rugosa Rorug03G0136200 Rorug03G0136300
rosa_samantha Rh3AG186800 Rh3BG215400 Rh3CG212100 Rh3DG211300
rosa_wichuraiana Rw3G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 267, 442
AciI CCGC 2 cut(s) 335, 1058
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 3 cut(s) 336, 409, 506
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 3 cut(s) 372, 450, 482
AfaI GTAC 6 cut(s) 75, 476, 516, 702, 875, 1004
AfiI CCNNNNNNNGG 2 cut(s) 267, 442
AflIII ACRYGT 1 cut(s) 167
AgeI ACCGGT 1 cut(s) 767
AgsI TTSAA 4 cut(s) 9, 86, 529, 968
AjiI CACGTC 1 cut(s) 148
AluBI AGCT 7 cut(s) 238, 247, 352, 496, 580, 896, 1095
AluI AGCT 7 cut(s) 238, 247, 352, 496, 580, 896, 1095
Alw21I GWGCWC 2 cut(s) 734, 891
Alw26I GTCTC 1 cut(s) 594
AlwI GGATC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 442
AoxI GGCC 4 cut(s) 336, 409, 506, 631
ApoI RAATTY 1 cut(s) 524
AsiGI ACCGGT 1 cut(s) 767
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 676, 757
AvaII GGWCC 1 cut(s) 115
AxyI CCTNAGG 1 cut(s) 131
BalI TGGCCA 1 cut(s) 508
BauI CACGAG 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 150, 438
Bbv12I GWGCWC 2 cut(s) 734, 891
BceAI ACGGC 1 cut(s) 424
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 594
BfaI CTAG 2 cut(s) 552, 704
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BmcAI AGTACT 1 cut(s) 702
Bme18I GGWCC 1 cut(s) 115
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 115
BmiI GGNNCC 1 cut(s) 117
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 150, 438
BplI GAGNNNNNCTC 2 cut(s) 1014, 1046
BpuEI CTTGAG 1 cut(s) 872
Bsa29I ATCGAT 1 cut(s) 596
BsaBI GATNNNNATC 2 cut(s) 194, 467
BsaWI WCCGGW 1 cut(s) 767
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 442
Bse118I RCCGGY 1 cut(s) 767
Bse1I ACTGG 2 cut(s) 71, 643
Bse21I CCTNAGG 1 cut(s) 131
Bse8I GATNNNNATC 2 cut(s) 194, 467
BseCI ATCGAT 1 cut(s) 596
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 2 cut(s) 194, 467
BseLI CCNNNNNNNGG 2 cut(s) 267, 442
BseMII CTCAG 3 cut(s) 177, 701, 1045
BseNI ACTGG 2 cut(s) 71, 643
BseRI GAGGAG 3 cut(s) 248, 590, 804
BshFI GGCC 4 cut(s) 338, 411, 508, 633
BshTI ACCGGT 1 cut(s) 767
BshVI ATCGAT 1 cut(s) 596
BsiHKAI GWGCWC 2 cut(s) 734, 891
BsiSI CCGG 1 cut(s) 768
BslFI GGGAC 1 cut(s) 101
BslI CCNNNNNNNGG 2 cut(s) 267, 442
BsmAI GTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 101
BsnI GGCC 4 cut(s) 338, 411, 508, 633
Bsp1286I GDGCHC 2 cut(s) 734, 891
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 3 cut(s) 16, 103, 1086
BspACI CCGC 2 cut(s) 335, 1058
BspANI GGCC 4 cut(s) 338, 411, 508, 633
BspCNI CTCAG 3 cut(s) 178, 702, 1044
BspDI ATCGAT 1 cut(s) 596
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 117
BspPI GGATC 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 767
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 2 cut(s) 71, 643
BssAI RCCGGY 1 cut(s) 767
BssMI GATC 3 cut(s) 16, 103, 1086
BssSI CACGAG 1 cut(s) 727
Bst2BI CACGAG 1 cut(s) 727
Bst4CI ACNGT 2 cut(s) 94, 1038
Bst6I CTCTTC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 1002
BstC8I GCNNGC 2 cut(s) 510, 1056
BstDEI CTNAG 4 cut(s) 131, 186, 710, 1031
BstF5I GGATG 1 cut(s) 334
BstKTI GATC 3 cut(s) 19, 106, 1089
BstMAI GTCTC 1 cut(s) 594
BstMBI GATC 3 cut(s) 16, 103, 1086
BstMWI GCNNNNNNNGC 2 cut(s) 244, 1064
BstNSI RCATGY 2 cut(s) 171, 1058
BstV2I GAAGAC 2 cut(s) 150, 438
Bsu15I ATCGAT 1 cut(s) 596
Bsu36I CCTNAGG 1 cut(s) 131
BsuRI GGCC 4 cut(s) 338, 411, 508, 633
BsuTUI ATCGAT 1 cut(s) 596
BtrI CACGTC 1 cut(s) 148
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 2 cut(s) 510, 1056
CaiI CAGNNNCTG 1 cut(s) 442
CciI TCATGA 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 767
Cfr13I GGNCC 1 cut(s) 115
ClaI ATCGAT 1 cut(s) 596
Csp6I GTAC 6 cut(s) 74, 475, 515, 701, 874, 1003
CspAI ACCGGT 1 cut(s) 767
CviQI GTAC 6 cut(s) 74, 475, 515, 701, 874, 1003
DdeI CTNAG 4 cut(s) 131, 186, 710, 1031
DpnI GATC 3 cut(s) 18, 105, 1088
DpnII GATC 3 cut(s) 16, 103, 1086
DraI TTTAAA 1 cut(s) 219
EaeI YGGCCR 3 cut(s) 336, 409, 506
Eam1104I CTCTTC 1 cut(s) 357
EarI CTCTTC 1 cut(s) 357
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 3 cut(s) 372, 450, 482
Eco81I CCTNAGG 1 cut(s) 131
EcoT22I ATGCAT 1 cut(s) 460
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 1 cut(s) 101
FauI CCCGC 1 cut(s) 1051
FauNDI CATATG 1 cut(s) 996
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 336
FspBI CTAG 2 cut(s) 552, 704
GluI GCNGC 1 cut(s) 336
HaeIII GGCC 4 cut(s) 338, 411, 508, 633
HapII CCGG 1 cut(s) 768
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 8 cut(s) 190, 273, 439, 463, 620, 712, 778, 989
HpaII CCGG 1 cut(s) 768
HphI GGTGA 2 cut(s) 676, 757
Hpy166II GTNNAC 3 cut(s) 475, 667, 716
Hpy188I TCNGA 8 cut(s) 187, 391, 430, 454, 724, 783, 1016, 1034
Hpy188III TCNNGA 7 cut(s) 14, 86, 173, 359, 443, 539, 851
Hpy8I GTNNAC 3 cut(s) 475, 667, 716
HpyAV CCTTC 5 cut(s) 29, 319, 396, 610, 1057
HpyCH4III ACNGT 2 cut(s) 94, 1038
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 7 cut(s) 47, 161, 316, 458, 751, 775, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 1064
HpyF3I CTNAG 4 cut(s) 131, 186, 710, 1031
HpySE526I ACGT 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 16, 103, 1086
LmnI GCTCC 3 cut(s) 235, 577, 689
LweI GCATC 1 cut(s) 445
MaeI CTAG 2 cut(s) 552, 704
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 4 cut(s) 22, 763, 898, 990
MalI GATC 3 cut(s) 18, 105, 1088
MboI GATC 3 cut(s) 16, 103, 1086
MboII GAAGA 6 cut(s) 155, 374, 379, 443, 1057, 1096
MhlI GDGCHC 2 cut(s) 734, 891
MlsI TGGCCA 1 cut(s) 508
MluCI AATT 3 cut(s) 524, 813, 972
MluNI TGGCCA 1 cut(s) 508
MlyI GAGTC 2 cut(s) 721, 998
MmeI TCCRAC 1 cut(s) 820
Mox20I TGGCCA 1 cut(s) 508
Mph1103I ATGCAT 1 cut(s) 460
MscI TGGCCA 1 cut(s) 508
MseI TTAA 3 cut(s) 218, 240, 561
MslI CAYNNNNRTG 6 cut(s) 417, 608, 746, 954, 1046, 1076
Msp20I TGGCCA 1 cut(s) 508
MspI CCGG 1 cut(s) 768
MwoI GCNNNNNNNGC 2 cut(s) 244, 1064
NdeI CATATG 1 cut(s) 996
NdeII GATC 3 cut(s) 16, 103, 1086
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 4 cut(s) 22, 763, 898, 990
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 2 cut(s) 171, 1058
PaeI GCATGC 1 cut(s) 1058
PagI TCATGA 1 cut(s) 172
PciI ACATGT 1 cut(s) 167
PfeI GAWTC 6 cut(s) 190, 273, 439, 463, 620, 778
PflFI GACNNNGTC 2 cut(s) 711, 761
PflMI CCANNNNNTGG 2 cut(s) 267, 442
PinAI ACCGGT 1 cut(s) 767
PkrI GCNGC 1 cut(s) 337
PleI GAGTC 2 cut(s) 720, 997
PpsI GAGTC 2 cut(s) 720, 997
PscI ACATGT 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 442
PsyI GACNNNGTC 2 cut(s) 711, 761
RsaI GTAC 6 cut(s) 75, 476, 516, 702, 875, 1004
RsaNI GTAC 6 cut(s) 74, 475, 515, 701, 874, 1003
RseI CAYNNNNRTG 6 cut(s) 417, 608, 746, 954, 1046, 1076
SaqAI TTAA 3 cut(s) 218, 240, 561
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 16, 103, 1086
Sau96I GGNCC 1 cut(s) 115
ScaI AGTACT 1 cut(s) 702
SchI GAGTC 2 cut(s) 721, 998
SduI GDGCHC 2 cut(s) 734, 891
SfaNI GCATC 1 cut(s) 445
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 6 cut(s) 417, 608, 746, 954, 1046, 1076
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
SphI GCATGC 1 cut(s) 1058
Sse9I AATT 3 cut(s) 524, 813, 972
SsiI CCGC 2 cut(s) 335, 1058
SspI AATATT 1 cut(s) 82
SspMI CTAG 2 cut(s) 552, 704
TaaI ACNGT 2 cut(s) 94, 1038
TaiI ACGT 1 cut(s) 150
TaqI TCGA 3 cut(s) 596, 807, 1089
TasI AATT 3 cut(s) 524, 813, 972
TatI WGTACW 3 cut(s) 700, 873, 1002
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 6 cut(s) 190, 273, 439, 463, 620, 778
Tru1I TTAA 3 cut(s) 218, 240, 561
Tru9I TTAA 3 cut(s) 218, 240, 561
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 4 cut(s) 22, 763, 898, 990
Tsp45I GTSAC 4 cut(s) 22, 763, 898, 990
TspDTI ATGAA 3 cut(s) 338, 1058, 1064
TspRI CASTG 1 cut(s) 99
Tth111I GACNNNGTC 2 cut(s) 711, 761
Van91I CCANNNNNTGG 2 cut(s) 267, 442
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 524
XceI RCATGY 2 cut(s) 171, 1058
XspI CTAG 2 cut(s) 552, 704
ZrmI AGTACT 1 cut(s) 702
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.