MD14G1023800.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013403
Physical Location & Seq
Forward (+)
2151861 .. 2154807
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1023800.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGCCGGAAGAAAGAGGAAGCTGAAATCGTTAGCTGAAACAATCACAAATAGTAACAGAAAAGCAAGCAAGATGAAAAAGGATAAACTGGAGCTGCAGACTTGGTCTGACCTTCCTGCCGAACTTTTGGAACTCATTGTGTCTACTTTAACTCTGCGGGATAACATTCGCGTATCTGTTGTATGCAAGAGATGGCACTCAGCTGCCATTTCTGTGCGGGTGGTCAACCAATCACCACTGCTTATGTACTTTCCCAGATTTGGTGACCTATATGAATTCTATGATCCATCGCAGCGCAAAATCTACTCCTTGGAGTTACCTGAGCTGACTGGGTCTAGGATTTGCTACACTAAAGACGGTTGGCTACTGTTATACAGACCTAGATCTCACCGCGTGTTCTTCTTTAATCCCTTCAATCGCAAGATAATAAAGGTACCAAGATTCGAGTTGACTTATCAGATAGTTGCATTCTCTTGTGCCCCAACTTCTGCCAGCTGCATACTGTTTACGGTGAAACACATCAGCCCCACAGTTGTTGCTATTAGCACTTGTCGTCCTGGGGAAAGAGAGTGGACTACTGTTAATTTCCAAAACCGGTTGCCCTTTGTGAGTAGCATTTGGAACAAGCTTGTTTTCTGCAACGGACTCTTTTATTGTCTGAGCCTCACCGGTTGGCTAGGGGTATTCAACCCCGAAGAACGCACTTGGAGTGTTCTTGCCGTTCCTCCACCCAAGTGCCCGGAGAACTTTTTTGCCAAAAATTGGTGGAAAGGTAAGTTTATGGCTGAGCATGAGGGAAACATATTAGTTATATATACATGTTCAAGTGAAAACCCGATTATATTTAAGCTGGATCAGATGAACATGGTTTGGGAAGAAATGATGACCCTCGATGGTCTGACACTGTTTGCGAGTTTCTTGTCATCTCATTCAAGAATTGACCTGCCTGGAATCATGAGAAACAGCGTCTACTTCTCGAAAGTTCGTTTCTACGGAAGGTGTTGCATATCCTACTCTCTCGACTCTTGTAGATACCATCCTCGCAAGGAGTGTCATGACTGGGGAGAGCAAGATCCTTTTAAGAAGATTTGGATTGAAGCACCTCAAGACTTCTCAACCTTAAATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like

Protein Analysis

377

Amino Acids

43.93

Weight (kDa)

9.25

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 35 - 69 6.6e-08 F-box-like
F-box PF00646 35 - 73 5.2e-08 F-box domain
Beta-prop_KIB1-4 PF03478 93 - 327 2e-30 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57790
fragaria_vesca FvH4_6g18890
malus_domestica MD12G1025100.v1.1 MD14G1023800.v1.1
prunus_persica Prupe.7G109000_v2.0.a1
pyrus_communis pycom12g02460 pycom14g02290
rosa_chinensis RchiOBHm_Chr3g0473881
rosa_laevigata RLG00000023976
rosa_multiflora Rmu_sc0002414.1_g000044
rosa_roxburghii Rroxscaffold_6G00407700
rosa_rugosa Rorug03G0136200 Rorug03G0136300
rosa_samantha Rh3AG186800 Rh3BG215400 Rh3CG212100 Rh3DG211300
rosa_wichuraiana Rw3G017080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 951
Acc65I GGTACC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 433
AccB7I CCANNNNNTGG 1 cut(s) 762
AccI GTMKAC 2 cut(s) 143, 969
AccII CGCG 2 cut(s) 171, 393
AciI CCGC 3 cut(s) 157, 217, 391
AclWI GGATC 3 cut(s) 278, 861, 1067
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 2 cut(s) 248, 435
AfiI CCNNNNNNNGG 3 cut(s) 260, 762, 1125
AflIII ACRYGT 1 cut(s) 818
AgeI ACCGGT 2 cut(s) 594, 668
AgsI TTSAA 5 cut(s) 415, 688, 825, 933, 1097
AjnI CCWGG 2 cut(s) 556, 946
AleI CACNNNNGTG 1 cut(s) 733
AluBI AGCT 8 cut(s) 22, 35, 94, 203, 325, 495, 628, 850
AluI AGCT 8 cut(s) 22, 35, 94, 203, 325, 495, 628, 850
AlwI GGATC 3 cut(s) 278, 861, 1067
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 94, 203, 292, 495
ApoI RAATTY 1 cut(s) 275
AsiGI ACCGGT 2 cut(s) 594, 668
Asp718I GGTACC 1 cut(s) 433
AspLEI GCGC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 740
AsuHPI GGTGA 5 cut(s) 225, 275, 380, 523, 658
BaeGI GKGCMC 2 cut(s) 481, 740
BanI GGYRCC 1 cut(s) 433
BbvI GCAGC 4 cut(s) 81, 190, 304, 482
BccI CCATC 4 cut(s) 186, 295, 887, 1044
BceAI ACGGC 1 cut(s) 704
BciT130I CCWGG 2 cut(s) 558, 948
BcnI CCSGG 1 cut(s) 740
BfaI CTAG 3 cut(s) 336, 381, 677
BfmI CTRYAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 951
BglII AGATCT 1 cut(s) 383
BisI GCNGC 4 cut(s) 95, 204, 293, 496
BlpI GCTNAGC 1 cut(s) 786
BlsI GCNGC 4 cut(s) 96, 205, 294, 497
Bme1390I CCNGG 3 cut(s) 558, 740, 948
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 558, 740, 948
BmrI ACTGGG 2 cut(s) 339, 1069
BmuI ACTGGG 2 cut(s) 339, 1069
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 1089
BpuMI CCSGG 1 cut(s) 740
BsaJI CCNNGG 2 cut(s) 309, 557
BsaWI WCCGGW 2 cut(s) 594, 668
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 762, 1125
Bse118I RCCGGY 2 cut(s) 594, 668
Bse1I ACTGG 3 cut(s) 93, 334, 1064
BseBI CCWGG 2 cut(s) 558, 948
BseDI CCNNGG 2 cut(s) 309, 557
BseGI GGATG 1 cut(s) 1036
BseLI CCNNNNNNNGG 3 cut(s) 260, 762, 1125
BseMII CTCAG 4 cut(s) 213, 312, 650, 777
BseNI ACTGG 3 cut(s) 93, 334, 1064
BseSI GKGCMC 2 cut(s) 481, 740
BseXI GCAGC 4 cut(s) 81, 190, 304, 482
Bsh1236I CGCG 2 cut(s) 171, 393
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 433
BshTI ACCGGT 2 cut(s) 594, 668
BsiSI CCGG 4 cut(s) 6, 595, 669, 740
BslI CCNNNNNNNGG 3 cut(s) 260, 762, 1125
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 481, 740
Bsp143I GATC 4 cut(s) 283, 383, 853, 1072
Bsp1720I GCTNAGC 1 cut(s) 786
BspACI CCGC 3 cut(s) 157, 217, 391
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 212, 313, 651, 778
BspFNI CGCG 2 cut(s) 171, 393
BspHI TCATGA 2 cut(s) 954, 1054
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 3 cut(s) 278, 861, 1067
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 2 cut(s) 594, 668
BsrI ACTGG 3 cut(s) 93, 334, 1064
BssAI RCCGGY 2 cut(s) 594, 668
BssECI CCNNGG 2 cut(s) 309, 557
BssMI GATC 4 cut(s) 283, 383, 853, 1072
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 558, 948
Bst4CI ACNGT 7 cut(s) 359, 369, 504, 511, 532, 580, 906
BstC8I GCNNGC 2 cut(s) 67, 493
BstDEI CTNAG 4 cut(s) 199, 321, 659, 786
BstEII GGTNACC 1 cut(s) 263
BstF5I GGATG 1 cut(s) 1036
BstFNI CGCG 2 cut(s) 171, 393
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 4 cut(s) 286, 386, 856, 1075
BstMBI GATC 4 cut(s) 283, 383, 853, 1072
BstNI CCWGG 2 cut(s) 558, 948
BstNSI RCATGY 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 3 cut(s) 556, 738, 946
BstSFI CTRYAG 1 cut(s) 95
BstSLI GKGCMC 2 cut(s) 481, 740
BstUI CGCG 2 cut(s) 171, 393
BstV1I GCAGC 4 cut(s) 81, 190, 304, 482
BstX2I RGATCY 2 cut(s) 383, 1072
BstYI RGATCY 2 cut(s) 383, 1072
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 1036
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 2 cut(s) 236, 902
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 2 cut(s) 67, 493
CciI TCATGA 2 cut(s) 954, 1054
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 594, 668
CseI GACGC 1 cut(s) 955
Csp6I GTAC 2 cut(s) 247, 434
CspAI ACCGGT 2 cut(s) 594, 668
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 5 cut(s) 791, 819, 865, 955, 1055
CviQI GTAC 2 cut(s) 247, 434
DdeI CTNAG 4 cut(s) 199, 321, 659, 786
DpnI GATC 4 cut(s) 285, 385, 855, 1074
DpnII GATC 4 cut(s) 283, 383, 853, 1072
DraIII CACNNNGTG 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 309
Eco91I GGTNACC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 556, 946
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 5 cut(s) 794, 822, 868, 958, 1058
FatI CATG 5 cut(s) 790, 818, 864, 954, 1054
FauI CCCGC 2 cut(s) 150, 210
FblI GTMKAC 2 cut(s) 143, 969
Fnu4HI GCNGC 4 cut(s) 95, 204, 293, 496
FokI GGATG 1 cut(s) 1023
Fsp4HI GCNGC 4 cut(s) 95, 204, 293, 496
FspBI CTAG 3 cut(s) 336, 381, 677
GlaI GCGC 1 cut(s) 296
GluI GCNGC 4 cut(s) 95, 204, 293, 496
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 6, 595, 669, 740
HgaI GACGC 1 cut(s) 955
HhaI GCGC 1 cut(s) 297
Hin1II CATG 5 cut(s) 794, 822, 868, 958, 1058
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 2 cut(s) 226, 450
HindII GTYRAC 2 cut(s) 226, 450
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 4 cut(s) 441, 645, 951, 1022
HpaII CCGG 4 cut(s) 6, 595, 669, 740
HphI GGTGA 5 cut(s) 225, 275, 380, 523, 658
Hpy166II GTNNAC 6 cut(s) 144, 226, 450, 507, 573, 970
Hpy188I TCNGA 5 cut(s) 109, 459, 660, 858, 900
Hpy188III TCNNGA 6 cut(s) 933, 955, 976, 1019, 1055, 1106
Hpy8I GTNNAC 6 cut(s) 144, 226, 450, 507, 573, 970
HpyAV CCTTC 3 cut(s) 122, 421, 990
HpyCH4III ACNGT 7 cut(s) 359, 369, 504, 511, 532, 580, 906
HpyCH4V TGCA 6 cut(s) 97, 186, 467, 498, 639, 1005
HpyF3I CTNAG 4 cut(s) 199, 321, 659, 786
Hsp92II CATG 5 cut(s) 794, 822, 868, 958, 1058
HspAI GCGC 1 cut(s) 295
KpnI GGTACC 1 cut(s) 437
Kzo9I GATC 4 cut(s) 283, 383, 853, 1072
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 4 cut(s) 81, 190, 304, 482
MaeI CTAG 3 cut(s) 336, 381, 677
MaeIII GTNAC 3 cut(s) 53, 263, 315
MalI GATC 4 cut(s) 285, 385, 855, 1074
MboI GATC 4 cut(s) 283, 383, 853, 1072
MboII GAAGA 5 cut(s) 21, 391, 707, 887, 1096
MflI RGATCY 2 cut(s) 383, 1072
MhlI GDGCHC 2 cut(s) 481, 740
MluCI AATT 5 cut(s) 275, 583, 760, 936, 1123
MlyI GAGTC 2 cut(s) 639, 1016
MnlI CCTC 7 cut(s) 9, 674, 735, 787, 899, 1050, 1113
MseI TTAA 6 cut(s) 149, 405, 582, 846, 1080, 1121
MslI CAYNNNNRTG 2 cut(s) 212, 733
MspA1I CMGCKG 2 cut(s) 203, 495
MspI CCGG 4 cut(s) 6, 595, 669, 740
MspR9I CCNGG 3 cut(s) 558, 740, 948
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 2 cut(s) 558, 948
MvnI CGCG 2 cut(s) 171, 393
NciI CCSGG 1 cut(s) 740
NdeII GATC 4 cut(s) 283, 383, 853, 1072
NlaIII CATG 5 cut(s) 794, 822, 868, 958, 1058
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 263
NspI RCATGY 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 733
PagI TCATGA 2 cut(s) 954, 1054
PciI ACATGT 1 cut(s) 818
PctI GAATGC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 441, 951
PflFI GACNNNGTC 2 cut(s) 103, 331
PflMI CCANNNNNTGG 1 cut(s) 762
PinAI ACCGGT 2 cut(s) 594, 668
PkrI GCNGC 4 cut(s) 96, 205, 294, 497
PleI GAGTC 2 cut(s) 639, 1016
PpsI GAGTC 2 cut(s) 639, 1016
PscI ACATGT 1 cut(s) 818
Psp6I CCWGG 2 cut(s) 556, 946
PspEI GGTNACC 1 cut(s) 263
PspGI CCWGG 2 cut(s) 556, 946
PspN4I GGNNCC 1 cut(s) 435
PstI CTGCAG 1 cut(s) 99
PsuI RGATCY 2 cut(s) 383, 1072
PsyI GACNNNGTC 2 cut(s) 103, 331
PvuII CAGCTG 2 cut(s) 203, 495
RsaI GTAC 2 cut(s) 248, 435
RsaNI GTAC 2 cut(s) 247, 434
RseI CAYNNNNRTG 2 cut(s) 212, 733
SaqAI TTAA 6 cut(s) 149, 405, 582, 846, 1080, 1121
SatI GCNGC 4 cut(s) 95, 204, 293, 496
Sau3AI GATC 4 cut(s) 283, 383, 853, 1072
SchI GAGTC 2 cut(s) 639, 1016
ScrFI CCNGG 3 cut(s) 558, 740, 948
SduI GDGCHC 2 cut(s) 481, 740
SfcI CTRYAG 1 cut(s) 95
SmiMI CAYNNNNRTG 2 cut(s) 212, 733
SmlI CTYRAG 1 cut(s) 1104
SmoI CTYRAG 1 cut(s) 1104
Sse9I AATT 5 cut(s) 275, 583, 760, 936, 1123
SsiI CCGC 3 cut(s) 157, 217, 391
SspMI CTAG 3 cut(s) 336, 381, 677
StyD4I CCNGG 3 cut(s) 556, 738, 946
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 7 cut(s) 359, 369, 504, 511, 532, 580, 906
TaqI TCGA 4 cut(s) 444, 891, 977, 1020
TasI AATT 5 cut(s) 275, 583, 760, 936, 1123
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 2 cut(s) 441, 951
Tru1I TTAA 6 cut(s) 149, 405, 582, 846, 1080, 1121
Tru9I TTAA 6 cut(s) 149, 405, 582, 846, 1080, 1121
TscAI CASTG 2 cut(s) 243, 909
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 4 cut(s) 94, 203, 292, 495
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 89, 288, 875
TspGWI ACGGA 2 cut(s) 657, 1008
TspRI CASTG 2 cut(s) 243, 909
Tth111I GACNNNGTC 2 cut(s) 103, 331
Van91I CCANNNNNTGG 1 cut(s) 762
XapI RAATTY 1 cut(s) 275
XceI RCATGY 1 cut(s) 822
XmiI GTMKAC 2 cut(s) 143, 969
XspI CTAG 3 cut(s) 336, 381, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.