MD12G1032000.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004708
Physical Location & Seq
Reverse (-)
3512242 .. 3516659
4418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1032000.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGTCCATAAACCATTTCTCCTCAGACCTCTCAGAAACCCAGTGGTGGAATGCTAATTCTAACCCCCACCGACAAGAAGACCCCTCAAGACACCCACACAGCAGAACCCATTTCATCCCCCAATTTTATCAAGATACCCACCACCACAGCCACGCCCACCACAGTTTCCAACCCTGGCAGCTCAACTCCCAAGACCCCACCACACCCATGTCCCCCACCCATTTCCATACCCCCACCGCTACCCTCAATTTCAACCTCAACCACCACGACGCCGTCGACCCCAACGAATCAGGCCAACTCCAGTCCCCGCCGCCGCATCACCGCGTCAACTCTGACGACTCCGACGACGAAAAAGAGCCCATGTTCGAGAAGCCCTTGACCCCAAGCGACGTCGGCAAGCTGAACCGCCTCGTGATTCCCAAGCAGCACGCAGAGAAGTACTTCCCGCTCGGCAGCGGCGACTCGGGCCTCCTGCTGAGTTTCGAGGACGAGTCCGGGAAGTCGTGGCGGTTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTCCTCACCAAGGGCTGGAGCCGTTACGTCAAGGAAAAGCGCCTCAACGCCGGCGACGTCGTTTTGTTCGAGCGGCGCCGGGCTAACACGGATCGACTCTCCATCGGCTGGAGACGCCGCAACGCGGTCCCAGCGCACGATAGTGGGGCTGGAGGGGTTGAGAGTAGCAGTGGCGGGGTTGGAGGGAATAGTAGTGGTGGTGGCGGGGGTTGGACAAGGATGTTCTATTCTGCGCCGCATCACGGCTCGTCTTATCCTGCGCACCAGTATCATCATAGTGTGGCGCCATACCCACCTGACTGTCTACATGCAGCAGGGTCCCGTGTCCAGAATATGAACCCAACGGCGTCAGTTGGGAACTCGAAGATACTGAGACTATTTGGAGTGAACTTGGAGTGCCAGCAGCAGGCAGCTGAGCACCTCGAGTCGGAACCATCAACCTCAGATGGCTCGTCGTCGACACTGTCTATGTCAAGCCAGGGTCCGACTCACGGCCAGCGGCAAAGGTACCCTCGTGCAGCTTATAATTATGCCGACCAACACAGGGAATTCACTAGTTTCTCTGGAGATGTCAACCCCATGAGATCAAATCGCCGAGGATAA

Protein Analysis

383

Amino Acids

42.84

Weight (kDa)

7.19

Isoelectric Point (pI)

54.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 122 - 223 2.5e-27 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1071
AatII GACGTC 2 cut(s) 393, 606
Acc16I TGCGCA 1 cut(s) 807
Acc65I GGTACC 1 cut(s) 1053
AccB1I GGYRCC 3 cut(s) 621, 829, 1053
AccB7I CCANNNNNTGG 2 cut(s) 561, 654
AccBSI CCGCTC 2 cut(s) 448, 619
AccI GTMKAC 3 cut(s) 276, 850, 1004
AccII CGCG 2 cut(s) 324, 671
AclWI GGATC 1 cut(s) 645
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 1 cut(s) 1094
AcyI GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
AfaI GTAC 2 cut(s) 440, 1055
AgsI TTSAA 1 cut(s) 253
AhlI ACTAGT 1 cut(s) 1100
AjnI CCWGG 2 cut(s) 173, 1023
AleI CACNNNNGTG 1 cut(s) 687
AluBI AGCT 5 cut(s) 181, 400, 543, 959, 1067
AluI AGCT 5 cut(s) 181, 400, 543, 959, 1067
Alw21I GWGCWC 1 cut(s) 966
Alw26I GTCTC 2 cut(s) 652, 913
AlwI GGATC 1 cut(s) 645
Ama87I CYCGRG 2 cut(s) 463, 968
AoxI GGCC 3 cut(s) 292, 466, 1039
ApeKI GCWGC 8 cut(s) 178, 424, 453, 534, 857, 949, 956, 1064
ApoI RAATTY 1 cut(s) 1094
Asp700I GAANNNNTTC 1 cut(s) 440
Asp718I GGTACC 1 cut(s) 1053
AspLEI GCGC 6 cut(s) 588, 624, 682, 781, 808, 832
AspS9I GGNCC 4 cut(s) 466, 673, 864, 1028
AsuC2I CCSGG 2 cut(s) 496, 626
AsuHPI GGTGA 2 cut(s) 311, 544
AvaI CYCGRG 2 cut(s) 463, 968
AvaII GGWCC 3 cut(s) 673, 864, 1028
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BanI GGYRCC 3 cut(s) 621, 829, 1053
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 2 cut(s) 410, 1059
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 966
BbvI GCAGC 8 cut(s) 190, 436, 465, 546, 869, 961, 968, 1076
BccI CCATC 3 cut(s) 656, 986, 988
BceAI ACGGC 5 cut(s) 257, 552, 805, 906, 1054
BciT130I CCWGG 2 cut(s) 175, 1025
BcnI CCSGG 2 cut(s) 496, 626
BcoDI GTCTC 2 cut(s) 652, 913
BcuI ACTAGT 1 cut(s) 1100
BfaI CTAG 1 cut(s) 1101
BfoI RGCGCY 3 cut(s) 589, 625, 833
BlpI GCTNAGC 1 cut(s) 960
BmcAI AGTACT 1 cut(s) 440
Bme1390I CCNGG 4 cut(s) 175, 496, 626, 1025
Bme18I GGWCC 3 cut(s) 673, 864, 1028
BmeT110I CYCGRG 2 cut(s) 463, 968
BmgT120I GGNCC 4 cut(s) 466, 673, 864, 1028
BmrFI CCNGG 4 cut(s) 175, 496, 626, 1025
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 2 cut(s) 325, 793
BmuI ACTGGG 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 5 cut(s) 284, 583, 676, 717, 1131
Bpu1102I GCTNAGC 1 cut(s) 960
BpuEI CTTGAG 1 cut(s) 70
BpuMI CCSGG 2 cut(s) 496, 626
BsaHI GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
BsaJI CCNNGG 4 cut(s) 173, 555, 1024, 1141
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bse118I RCCGGY 1 cut(s) 596
Bse1I ACTGG 4 cut(s) 40, 301, 530, 811
BseBI CCWGG 2 cut(s) 175, 1025
BseDI CCNNGG 4 cut(s) 173, 555, 1024, 1141
BseGI GGATG 2 cut(s) 114, 771
BseMII CTCAG 6 cut(s) 36, 45, 467, 908, 951, 1002
BseNI ACTGG 4 cut(s) 40, 301, 530, 811
BseRI GAGGAG 1 cut(s) 10
BseXI GCAGC 8 cut(s) 190, 436, 465, 546, 869, 961, 968, 1076
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 1 cut(s) 1083
Bsh1236I CGCG 2 cut(s) 324, 671
BshFI GGCC 3 cut(s) 294, 468, 1041
BshNI GGYRCC 3 cut(s) 621, 829, 1053
BsiHKAI GWGCWC 1 cut(s) 966
BsiHKCI CYCGRG 2 cut(s) 463, 968
BsiSI CCGG 3 cut(s) 495, 597, 625
BslFI GGGAC 4 cut(s) 196, 289, 659, 850
BsmAI GTCTC 2 cut(s) 652, 913
BsmBI CGTCTC 1 cut(s) 652
BsmFI GGGAC 4 cut(s) 196, 289, 659, 850
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 3 cut(s) 294, 468, 1041
BsoBI CYCGRG 2 cut(s) 463, 968
Bsp1286I GDGCHC 2 cut(s) 360, 966
Bsp143I GATC 2 cut(s) 637, 1130
Bsp1720I GCTNAGC 1 cut(s) 960
BspANI GGCC 3 cut(s) 294, 468, 1041
BspCNI CTCAG 6 cut(s) 35, 44, 468, 909, 952, 1001
BspFNI CGCG 2 cut(s) 324, 671
BspPI GGATC 1 cut(s) 645
BspT107I GGYRCC 3 cut(s) 621, 829, 1053
BsrBI CCGCTC 2 cut(s) 448, 619
BsrFI RCCGGY 1 cut(s) 596
BsrI ACTGG 4 cut(s) 40, 301, 530, 811
BssAI RCCGGY 1 cut(s) 596
BssECI CCNNGG 4 cut(s) 173, 555, 1024, 1141
BssMI GATC 2 cut(s) 637, 1130
BssNI GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
BssSI CACGAG 2 cut(s) 410, 1059
BssT1I CCWWGG 1 cut(s) 555
Bst2BI CACGAG 2 cut(s) 410, 1059
Bst2UI CCWGG 2 cut(s) 175, 1025
Bst4CI ACNGT 3 cut(s) 164, 848, 1011
BstACI GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
BstC8I GCNNGC 6 cut(s) 398, 429, 598, 947, 954, 1043
BstDEI CTNAG 6 cut(s) 22, 31, 476, 917, 960, 988
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 114, 771
BstFNI CGCG 2 cut(s) 324, 671
BstH2I RGCGCY 3 cut(s) 589, 625, 833
BstHHI GCGC 6 cut(s) 588, 624, 682, 781, 808, 832
BstKTI GATC 2 cut(s) 640, 1133
BstMAI GTCTC 2 cut(s) 652, 913
BstMBI GATC 2 cut(s) 637, 1130
BstMWI GCNNNNNNNGC 5 cut(s) 393, 465, 540, 660, 677
BstNI CCWGG 2 cut(s) 175, 1025
BstNSI RCATGY 1 cut(s) 857
BstSCI CCNGG 4 cut(s) 173, 494, 624, 1023
BstUI CGCG 2 cut(s) 324, 671
BstV1I GCAGC 8 cut(s) 190, 436, 465, 546, 869, 961, 968, 1076
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 3 cut(s) 294, 468, 1041
BtsCI GGATG 2 cut(s) 114, 771
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 3 cut(s) 47, 721, 1007
Cac8I GCNNGC 6 cut(s) 398, 429, 598, 947, 954, 1043
CfoI GCGC 6 cut(s) 588, 624, 682, 781, 808, 832
Cfr10I RCCGGY 1 cut(s) 596
Cfr13I GGNCC 4 cut(s) 466, 673, 864, 1028
CseI GACGC 4 cut(s) 278, 313, 669, 882
Csp6I GTAC 2 cut(s) 439, 1054
CviAII CATG 4 cut(s) 208, 361, 854, 1126
CviQI GTAC 2 cut(s) 439, 1054
DdeI CTNAG 6 cut(s) 22, 31, 476, 917, 960, 988
DinI GGCGCC 2 cut(s) 623, 831
DpnI GATC 2 cut(s) 639, 1132
DpnII GATC 2 cut(s) 637, 1130
EaeI YGGCCR 1 cut(s) 1039
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 3 cut(s) 673, 864, 1028
Eco88I CYCGRG 2 cut(s) 463, 968
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 1094
EcoRII CCWGG 2 cut(s) 173, 1023
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 360
EgeI GGCGCC 2 cut(s) 623, 831
EheI GGCGCC 2 cut(s) 623, 831
ErhI CCWWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 652
FaeI CATG 4 cut(s) 211, 364, 857, 1129
FaqI GGGAC 4 cut(s) 196, 289, 659, 850
FatI CATG 4 cut(s) 207, 360, 853, 1125
FauI CCCGC 4 cut(s) 315, 453, 713, 743
FblI GTMKAC 3 cut(s) 276, 850, 1004
FokI GGATG 2 cut(s) 101, 778
FriOI GRGCYC 1 cut(s) 360
FspBI CTAG 1 cut(s) 1101
FspI TGCGCA 1 cut(s) 807
GlaI GCGC 6 cut(s) 587, 623, 681, 780, 807, 831
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 5 cut(s) 284, 583, 676, 717, 1131
HaeII RGCGCY 3 cut(s) 589, 625, 833
HaeIII GGCC 3 cut(s) 294, 468, 1041
HapII CCGG 3 cut(s) 495, 597, 625
HgaI GACGC 4 cut(s) 278, 313, 669, 882
HhaI GCGC 6 cut(s) 588, 624, 682, 781, 808, 832
Hin1I GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
Hin1II CATG 4 cut(s) 211, 364, 857, 1129
Hin6I GCGC 6 cut(s) 586, 622, 680, 779, 806, 830
HinP1I GCGC 6 cut(s) 586, 622, 680, 779, 806, 830
HincII GTYRAC 4 cut(s) 277, 328, 1005, 1120
HindII GTYRAC 4 cut(s) 277, 328, 1005, 1120
HinfI GANTC 8 cut(s) 287, 338, 415, 461, 491, 642, 971, 1033
HpaII CCGG 3 cut(s) 495, 597, 625
HphI GGTGA 2 cut(s) 311, 544
Hpy166II GTNNAC 6 cut(s) 277, 328, 851, 934, 1005, 1120
Hpy188I TCNGA 7 cut(s) 25, 34, 334, 343, 976, 991, 1032
Hpy188III TCNNGA 6 cut(s) 87, 131, 367, 412, 874, 1110
Hpy8I GTNNAC 6 cut(s) 277, 328, 851, 934, 1005, 1120
HpyCH4III ACNGT 3 cut(s) 164, 848, 1011
HpyCH4IV ACGT 4 cut(s) 390, 546, 573, 603
HpyCH4V TGCA 2 cut(s) 857, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 393, 465, 540, 660, 677
HpyF3I CTNAG 6 cut(s) 22, 31, 476, 917, 960, 988
HpySE526I ACGT 4 cut(s) 390, 546, 573, 603
Hsp92I GRCGYC 7 cut(s) 270, 390, 603, 622, 661, 830, 893
Hsp92II CATG 4 cut(s) 211, 364, 857, 1129
HspAI GCGC 6 cut(s) 586, 622, 680, 779, 806, 830
KasI GGCGCC 2 cut(s) 621, 829
KflI GGGWCCC 1 cut(s) 864
KpnI GGTACC 1 cut(s) 1057
KroI GCCGGC 1 cut(s) 596
KroNI GCCGGC 1 cut(s) 598
Kzo9I GATC 2 cut(s) 637, 1130
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 8 cut(s) 190, 436, 465, 546, 869, 961, 968, 1076
LweI GCATC 2 cut(s) 325, 793
MaeI CTAG 1 cut(s) 1101
MaeII ACGT 4 cut(s) 390, 546, 573, 603
MaeIII GTNAC 1 cut(s) 569
MalI GATC 2 cut(s) 639, 1132
MbiI CCGCTC 2 cut(s) 448, 619
MboI GATC 2 cut(s) 637, 1130
MboII GAAGA 2 cut(s) 89, 922
MhlI GDGCHC 2 cut(s) 360, 966
MluCI AATT 5 cut(s) 55, 122, 247, 1072, 1094
Mly113I GGCGCC 2 cut(s) 622, 830
MlyI GAGTC 6 cut(s) 332, 455, 500, 636, 980, 1027
MmeI TCCRAC 6 cut(s) 193, 366, 706, 737, 954, 1055
MreI CGCCGGCG 1 cut(s) 596
MroNI GCCGGC 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 440
MslI CAYNNNNRTG 3 cut(s) 206, 687, 822
MspA1I CMGCKG 3 cut(s) 456, 959, 1045
MspI CCGG 3 cut(s) 495, 597, 625
MspR9I CCNGG 4 cut(s) 175, 496, 626, 1025
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 2 cut(s) 175, 1025
MvnI CGCG 2 cut(s) 324, 671
MwoI GCNNNNNNNGC 5 cut(s) 393, 465, 540, 660, 677
NaeI GCCGGC 1 cut(s) 598
NarI GGCGCC 2 cut(s) 622, 830
NciI CCSGG 2 cut(s) 496, 626
NdeII GATC 2 cut(s) 637, 1130
NgoMIV GCCGGC 1 cut(s) 596
NlaIII CATG 4 cut(s) 211, 364, 857, 1129
NmeAIII GCCGAG 1 cut(s) 429
NsbI TGCGCA 1 cut(s) 807
NspI RCATGY 1 cut(s) 857
OliI CACNNNNGTG 1 cut(s) 687
PaeR7I CTCGAG 1 cut(s) 968
PcsI WCGNNNNNNNCGW 6 cut(s) 273, 282, 342, 456, 600, 612
PctI GAATGC 1 cut(s) 55
PdiI GCCGGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 2 cut(s) 287, 415
PflFI GACNNNGTC 2 cut(s) 272, 1009
PflMI CCANNNNNTGG 2 cut(s) 561, 654
PfoI TCCNGGA 1 cut(s) 494
PleI GAGTC 6 cut(s) 332, 455, 499, 636, 979, 1027
PluTI GGCGCC 2 cut(s) 625, 833
PpsI GAGTC 6 cut(s) 332, 455, 499, 636, 979, 1027
PpuMI RGGWCCY 1 cut(s) 864
PsiI TTATAA 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 864
Psp6I CCWGG 2 cut(s) 173, 1023
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 173, 1023
PspPI GGNCC 4 cut(s) 466, 673, 864, 1028
PspPPI RGGWCCY 1 cut(s) 864
PspXI VCTCGAGB 1 cut(s) 968
PsyI GACNNNGTC 2 cut(s) 272, 1009
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 2 cut(s) 440, 1055
RsaNI GTAC 2 cut(s) 439, 1054
RseI CAYNNNNRTG 3 cut(s) 206, 687, 822
SalI GTCGAC 2 cut(s) 275, 1003
Sau3AI GATC 2 cut(s) 637, 1130
Sau96I GGNCC 4 cut(s) 466, 673, 864, 1028
ScaI AGTACT 1 cut(s) 440
SchI GAGTC 6 cut(s) 332, 455, 500, 636, 980, 1027
ScrFI CCNGG 4 cut(s) 175, 496, 626, 1025
SduI GDGCHC 2 cut(s) 360, 966
SfaNI GCATC 2 cut(s) 325, 793
SfoI GGCGCC 2 cut(s) 623, 831
Sfr274I CTCGAG 1 cut(s) 968
SgrAI CRCCGGYG 1 cut(s) 596
SinI GGWCC 3 cut(s) 673, 864, 1028
SlaI CTCGAG 1 cut(s) 968
SmiMI CAYNNNNRTG 3 cut(s) 206, 687, 822
SmlI CTYRAG 2 cut(s) 85, 968
SmoI CTYRAG 2 cut(s) 85, 968
SpeI ACTAGT 1 cut(s) 1100
Sse9I AATT 5 cut(s) 55, 122, 247, 1072, 1094
SspDI GGCGCC 2 cut(s) 621, 829
SspMI CTAG 1 cut(s) 1101
StyD4I CCNGG 4 cut(s) 173, 494, 624, 1023
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 3 cut(s) 164, 848, 1011
TaiI ACGT 4 cut(s) 393, 549, 576, 606
TaqI TCGA 8 cut(s) 276, 366, 483, 615, 640, 908, 969, 1004
TasI AATT 5 cut(s) 55, 122, 247, 1072, 1094
TatI WGTACW 1 cut(s) 438
TauI GCSGC 7 cut(s) 313, 316, 459, 622, 666, 784, 1048
TfiI GAWTC 2 cut(s) 287, 415
TscAI CASTG 3 cut(s) 47, 721, 1014
TseI GCWGC 8 cut(s) 178, 424, 453, 534, 857, 949, 956, 1064
TspDTI ATGAA 2 cut(s) 103, 896
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 3 cut(s) 47, 721, 1014
Tth111I GACNNNGTC 2 cut(s) 272, 1009
Van91I CCANNNNNTGG 2 cut(s) 561, 654
VpaK11BI GGWCC 3 cut(s) 673, 864, 1028
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 1094
XceI RCATGY 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 968
XmiI GTMKAC 3 cut(s) 276, 850, 1004
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 1 cut(s) 1101
ZraI GACGTC 2 cut(s) 391, 604
ZrmI AGTACT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.