MD14G1032300.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004708
Physical Location & Seq
Reverse (-)
2892810 .. 2896783
3974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1032300.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGTCCACAAACCACTTCTCCTCAGACCTCTCAGAAACCCAGTGGTGGAACACCTCCAGTGCTAATTCTAACCCCCACCAACAAGATGACTCCTCAGGACACCCCCACAGCAGAACCCATATCATCCCCCAATTTTATCAAGATGGTGGCAGCCACCCCCATCCCAGTTTCCAACCCTTGCAGCTCAACTCCCAAGACCCCTTCACTCCCATGTCCCCCACCCATTTCCACACCCCTACAACCACCTTCAATTTCAACCTCAACCACCACGACGCCATCGATCCCAACGAATCAGACCAACTCCAGCCCCCGCCGCCGCATCCCCGCATCGACTCCTACGACGAGAAAGAGCCCATGTTCGAGAAGCCCTTGACCCCCAGCGACGTCGGCAAGCTCAACCGCCTCGTGATTCCCAAGCAGCACGCGGAGAAGTACTTCCCGCTCGGCGGCGGAGACTCGGGCCTCCTGCTCAGTTTCGAGGACGAGTCCGGGAAGTCGTGGCGGTTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTCCTCACCAAGGGCTGGAGCCGGTACGTCAAGGAGAAGCGCCTCGACGCAGGCGACGTCGTTTCGTTCGAGCGGCTCCGGGCCCACACGGATCGACTCTTCATCGGCTGGAGACGCCGCAACGCGGTCGCGGCGCACGATAGCGGTGCTCAGGTTAGCGGAGGAGGGGTTGAGAGTAGCAGTGGTGGTGGCGGAGGTTGGACCAGGATGTTGTATCCTGCACCGCATCACGGCTCGTCTTATCCTGCGCACCACTACTGTCATGGTGTGGCGCCATACCCACCTGACTGTCTACATGCAGCAGGGTCTGATGTTCAGAATATGAACCAAACGACATCGGTTGGGAACTCGAAGGTACTGAGGCTATTTGGGGTGAACTTGGAGTGCCAGCAGCAGGCAGCTGAGCACCACGAGTCGGAACCATCGACCCCAGATGGCTCATCATCGAGTTTGTCGATGTCGAGCCAGGGTCCGACTCACGGCCAGCAACAATTGTACCCTTCTGCTGCTTATAATTATGCCGACCAACACAGGGAATTCACTACTTTCTCCGGAGATGTCAAACCAACGAGAAATCGCCGAGGATAG

Protein Analysis

376

Amino Acids

41.76

Weight (kDa)

6.32

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 120 - 221 4.1e-27 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1053
AatII GACGTC 2 cut(s) 387, 600
Acc16I TGCGCA 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 555
AccBSI CCGCTC 2 cut(s) 442, 613
AccI GTMKAC 1 cut(s) 832
AccII CGCG 3 cut(s) 425, 665, 671
AccIII TCCGGA 1 cut(s) 1091
AclWI GGATC 2 cut(s) 275, 639
AcoI YGGCCR 1 cut(s) 1021
AcsI RAATTY 1 cut(s) 1076
AcyI GRCGYC 5 cut(s) 273, 384, 597, 655, 812
AfaI GTAC 4 cut(s) 434, 566, 897, 1037
AgsI TTSAA 2 cut(s) 250, 256
AjnI CCWGG 2 cut(s) 743, 1005
AluBI AGCT 4 cut(s) 184, 394, 537, 941
AluI AGCT 4 cut(s) 184, 394, 537, 941
Alw21I GWGCWC 2 cut(s) 691, 948
Alw26I GTCTC 2 cut(s) 447, 646
AlwI GGATC 2 cut(s) 275, 639
AlwNI CAGNNNCTG 1 cut(s) 848
Ama87I CYCGRG 1 cut(s) 457
Aor13HI TCCGGA 1 cut(s) 1091
AoxI GGCC 3 cut(s) 460, 621, 1021
ApaI GGGCCC 1 cut(s) 625
ApeKI GCWGC 8 cut(s) 150, 181, 418, 528, 839, 931, 938, 1046
ApoI RAATTY 1 cut(s) 1076
Asp700I GAANNNNTTC 1 cut(s) 434
AspLEI GCGC 4 cut(s) 582, 676, 790, 814
AspS9I GGNCC 5 cut(s) 460, 621, 622, 741, 1010
AsuC2I CCSGG 2 cut(s) 490, 620
AsuHPI GGTGA 2 cut(s) 538, 925
AvaI CYCGRG 1 cut(s) 457
AvaII GGWCC 2 cut(s) 741, 1010
AxyI CCTNAGG 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 625
BaeI ACNNNNGTAYC 2 cut(s) 1019, 1052
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 2 cut(s) 354, 625
BauI CACGAG 2 cut(s) 404, 950
Bbv12I GWGCWC 2 cut(s) 691, 948
BbvI GCAGC 8 cut(s) 162, 193, 430, 540, 851, 943, 950, 1033
BccI CCATC 5 cut(s) 137, 168, 284, 968, 970
BceAI ACGGC 2 cut(s) 787, 1036
BcgI CGANNNNNNTGC 4 cut(s) 649, 668, 683, 702
BciT130I CCWGG 2 cut(s) 745, 1007
BciVI GTATCC 1 cut(s) 765
BcnI CCSGG 2 cut(s) 490, 620
BcoDI GTCTC 2 cut(s) 447, 646
BfoI RGCGCY 2 cut(s) 583, 815
BfuI GTATCC 1 cut(s) 765
BlpI GCTNAGC 1 cut(s) 942
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 4 cut(s) 490, 620, 745, 1007
Bme18I GGWCC 2 cut(s) 741, 1010
BmeT110I CYCGRG 1 cut(s) 457
BmgT120I GGNCC 5 cut(s) 460, 621, 622, 741, 1010
BmiI GGNNCC 7 cut(s) 506, 560, 617, 623, 813, 960, 1011
BmrFI CCNGG 4 cut(s) 490, 620, 745, 1007
BmrI ACTGGG 2 cut(s) 34, 159
BmsI GCATC 3 cut(s) 328, 336, 775
BmuI ACTGGG 2 cut(s) 34, 159
BpmI CTGGAG 4 cut(s) 40, 287, 577, 670
Bpu10I CCTNAGC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 490, 620
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 5 cut(s) 273, 384, 597, 655, 812
BsaJI CCNNGG 3 cut(s) 549, 1006, 1120
BsaWI WCCGGW 1 cut(s) 1091
BsaXI ACNNNNNCTCC 3 cut(s) 32, 693, 723
Bse118I RCCGGY 1 cut(s) 561
Bse1I ACTGG 4 cut(s) 40, 57, 165, 524
Bse21I CCTNAGG 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 1091
BseBI CCWGG 2 cut(s) 745, 1007
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 549, 1006, 1120
BseGI GGATG 4 cut(s) 123, 160, 319, 753
BseMII CTCAG 7 cut(s) 36, 45, 108, 484, 704, 890, 933
BseNI ACTGG 4 cut(s) 40, 57, 165, 524
BseRI GAGGAG 3 cut(s) 10, 82, 717
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 8 cut(s) 162, 193, 430, 540, 851, 943, 950, 1033
BseYI CCCAGC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 3 cut(s) 425, 665, 671
Bsh1285I CGRYCG 1 cut(s) 669
BshFI GGCC 3 cut(s) 462, 623, 1023
BshNI GGYRCC 1 cut(s) 811
BshVI ATCGAT 1 cut(s) 279
BsiEI CGRYCG 1 cut(s) 669
BsiHKAI GWGCWC 2 cut(s) 691, 948
BsiHKCI CYCGRG 1 cut(s) 457
BsiSI CCGG 4 cut(s) 489, 562, 619, 1092
BslFI GGGAC 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 447, 646
BsmBI CGTCTC 1 cut(s) 646
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 3 cut(s) 462, 623, 1023
BsoBI CYCGRG 1 cut(s) 457
Bsp120I GGGCCC 1 cut(s) 621
Bsp1286I GDGCHC 4 cut(s) 354, 625, 691, 948
Bsp13I TCCGGA 1 cut(s) 1091
Bsp143I GATC 2 cut(s) 280, 631
Bsp1720I GCTNAGC 1 cut(s) 942
BspANI GGCC 3 cut(s) 462, 623, 1023
BspCNI CTCAG 7 cut(s) 35, 44, 107, 483, 703, 891, 934
BspDI ATCGAT 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 1091
BspFNI CGCG 3 cut(s) 425, 665, 671
BspLI GGNNCC 7 cut(s) 506, 560, 617, 623, 813, 960, 1011
BspPI GGATC 2 cut(s) 275, 639
BspT107I GGYRCC 1 cut(s) 811
BsrBI CCGCTC 2 cut(s) 442, 613
BsrFI RCCGGY 1 cut(s) 561
BsrI ACTGG 4 cut(s) 40, 57, 165, 524
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 3 cut(s) 549, 1006, 1120
BssMI GATC 2 cut(s) 280, 631
BssNI GRCGYC 5 cut(s) 273, 384, 597, 655, 812
BssSI CACGAG 2 cut(s) 404, 950
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 2 cut(s) 404, 950
Bst2UI CCWGG 2 cut(s) 745, 1007
Bst4CI ACNGT 2 cut(s) 800, 830
Bst6I CTCTTC 1 cut(s) 644
BstACI GRCGYC 5 cut(s) 273, 384, 597, 655, 812
BstC8I GCNNGC 6 cut(s) 392, 423, 592, 929, 936, 1025
BstDEI CTNAG 7 cut(s) 22, 31, 94, 470, 690, 899, 942
BstENI CCTNNNNNAGG 1 cut(s) 548
BstF5I GGATG 4 cut(s) 123, 160, 319, 753
BstFNI CGCG 3 cut(s) 425, 665, 671
BstH2I RGCGCY 2 cut(s) 583, 815
BstHHI GCGC 4 cut(s) 582, 676, 790, 814
BstKTI GATC 2 cut(s) 283, 634
BstMAI GTCTC 2 cut(s) 447, 646
BstMBI GATC 2 cut(s) 280, 631
BstMCI CGRYCG 1 cut(s) 669
BstMWI GCNNNNNNNGC 5 cut(s) 313, 387, 534, 654, 671
BstNI CCWGG 2 cut(s) 745, 1007
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 4 cut(s) 488, 618, 743, 1005
BstSLI GKGCMC 1 cut(s) 625
BstUI CGCG 3 cut(s) 425, 665, 671
BstV1I GCAGC 8 cut(s) 162, 193, 430, 540, 851, 943, 950, 1033
Bsu15I ATCGAT 1 cut(s) 279
Bsu36I CCTNAGG 1 cut(s) 94
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 3 cut(s) 462, 623, 1023
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 4 cut(s) 123, 160, 319, 753
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 3 cut(s) 47, 64, 727
Cac8I GCNNGC 6 cut(s) 392, 423, 592, 929, 936, 1025
CaiI CAGNNNCTG 1 cut(s) 848
CfoI GCGC 4 cut(s) 582, 676, 790, 814
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 5 cut(s) 460, 621, 622, 741, 1010
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 3 cut(s) 281, 596, 663
Csp6I GTAC 4 cut(s) 433, 565, 896, 1036
CviAII CATG 4 cut(s) 211, 355, 803, 836
CviQI GTAC 4 cut(s) 433, 565, 896, 1036
DdeI CTNAG 7 cut(s) 22, 31, 94, 470, 690, 899, 942
DinI GGCGCC 1 cut(s) 813
DpnI GATC 2 cut(s) 282, 633
DpnII GATC 2 cut(s) 280, 631
EaeI YGGCCR 1 cut(s) 1021
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
EciI GGCGGA 2 cut(s) 465, 747
Eco130I CCWWGG 1 cut(s) 549
Eco24I GRGCYC 2 cut(s) 354, 625
Eco47I GGWCC 2 cut(s) 741, 1010
Eco81I CCTNAGG 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 457
EcoNI CCTNNNNNAGG 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 1076
EcoRII CCWGG 2 cut(s) 743, 1005
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 2 cut(s) 354, 625
EgeI GGCGCC 1 cut(s) 813
EheI GGCGCC 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 549
Esp3I CGTCTC 1 cut(s) 646
FaeI CATG 4 cut(s) 214, 358, 806, 839
FaiI YATR 9 cut(s) 120, 212, 356, 804, 817, 837, 863, 1053, 1059
FaqI GGGAC 1 cut(s) 199
FatI CATG 4 cut(s) 210, 354, 802, 835
FauI CCCGC 3 cut(s) 318, 332, 447
FblI GTMKAC 1 cut(s) 832
FokI GGATG 4 cut(s) 110, 147, 306, 760
FriOI GRGCYC 2 cut(s) 354, 625
FspI TGCGCA 1 cut(s) 789
GlaI GCGC 4 cut(s) 581, 675, 789, 813
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 4 cut(s) 40, 287, 577, 670
HaeII RGCGCY 2 cut(s) 583, 815
HaeIII GGCC 3 cut(s) 462, 623, 1023
HapII CCGG 4 cut(s) 489, 562, 619, 1092
HgaI GACGC 3 cut(s) 281, 596, 663
HhaI GCGC 4 cut(s) 582, 676, 790, 814
Hin1I GRCGYC 5 cut(s) 273, 384, 597, 655, 812
Hin1II CATG 4 cut(s) 214, 358, 806, 839
Hin6I GCGC 4 cut(s) 580, 674, 788, 812
HinP1I GCGC 4 cut(s) 580, 674, 788, 812
HinfI GANTC 9 cut(s) 89, 290, 332, 409, 455, 485, 636, 953, 1015
HpaII CCGG 4 cut(s) 489, 562, 619, 1092
HphI GGTGA 2 cut(s) 538, 925
Hpy166II GTNNAC 3 cut(s) 6, 833, 916
Hpy188I TCNGA 7 cut(s) 25, 34, 295, 850, 858, 958, 1014
Hpy188III TCNNGA 5 cut(s) 96, 140, 361, 406, 1092
Hpy8I GTNNAC 3 cut(s) 6, 833, 916
Hpy99I CGWCG 7 cut(s) 275, 344, 386, 389, 590, 599, 602
HpyAV CCTTC 4 cut(s) 211, 256, 886, 1050
HpyCH4III ACNGT 2 cut(s) 800, 830
HpyCH4IV ACGT 4 cut(s) 384, 540, 567, 597
HpyCH4V TGCA 3 cut(s) 181, 761, 839
HpyF10VI GCNNNNNNNGC 5 cut(s) 313, 387, 534, 654, 671
HpyF3I CTNAG 7 cut(s) 22, 31, 94, 470, 690, 899, 942
HpySE526I ACGT 4 cut(s) 384, 540, 567, 597
Hsp92I GRCGYC 5 cut(s) 273, 384, 597, 655, 812
Hsp92II CATG 4 cut(s) 214, 358, 806, 839
HspAI GCGC 4 cut(s) 580, 674, 788, 812
KasI GGCGCC 1 cut(s) 811
Kpn2I TCCGGA 1 cut(s) 1091
Kzo9I GATC 2 cut(s) 280, 631
LmnI GCTCC 2 cut(s) 558, 621
Lsp1109I GCAGC 8 cut(s) 162, 193, 430, 540, 851, 943, 950, 1033
LweI GCATC 3 cut(s) 328, 336, 775
MaeII ACGT 4 cut(s) 384, 540, 567, 597
MalI GATC 2 cut(s) 282, 633
MbiI CCGCTC 2 cut(s) 442, 613
MboI GATC 2 cut(s) 280, 631
MboII GAAGA 1 cut(s) 631
MfeI CAATTG 1 cut(s) 1031
MhlI GDGCHC 4 cut(s) 354, 625, 691, 948
MluCI AATT 6 cut(s) 64, 131, 250, 1031, 1054, 1076
Mly113I GGCGCC 1 cut(s) 812
MlyI GAGTC 7 cut(s) 83, 326, 449, 494, 630, 962, 1009
MmeI TCCRAC 4 cut(s) 196, 719, 936, 1037
MroI TCCGGA 1 cut(s) 1091
MroXI GAANNNNTTC 1 cut(s) 434
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 4 cut(s) 489, 562, 619, 1092
MspR9I CCNGG 4 cut(s) 490, 620, 745, 1007
MunI CAATTG 1 cut(s) 1031
MvaI CCWGG 2 cut(s) 745, 1007
MvnI CGCG 3 cut(s) 425, 665, 671
MwoI GCNNNNNNNGC 5 cut(s) 313, 387, 534, 654, 671
NarI GGCGCC 1 cut(s) 812
NciI CCSGG 2 cut(s) 490, 620
NdeII GATC 2 cut(s) 280, 631
NlaIII CATG 4 cut(s) 214, 358, 806, 839
NlaIV GGNNCC 7 cut(s) 506, 560, 617, 623, 813, 960, 1011
NmeAIII GCCGAG 1 cut(s) 423
NsbI TGCGCA 1 cut(s) 789
NspI RCATGY 1 cut(s) 839
PcsI WCGNNNNNNNCGW 9 cut(s) 276, 285, 336, 591, 594, 606, 675, 962, 992
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 2 cut(s) 290, 409
PflMI CCANNNNNTGG 1 cut(s) 555
PfoI TCCNGGA 1 cut(s) 488
PleI GAGTC 7 cut(s) 83, 326, 449, 493, 630, 961, 1009
PluTI GGCGCC 1 cut(s) 815
PpsI GAGTC 7 cut(s) 83, 326, 449, 493, 630, 961, 1009
PsiI TTATAA 1 cut(s) 1053
Psp6I CCWGG 2 cut(s) 743, 1005
PspFI CCCAGC 1 cut(s) 377
PspGI CCWGG 2 cut(s) 743, 1005
PspN4I GGNNCC 7 cut(s) 506, 560, 617, 623, 813, 960, 1011
PspOMI GGGCCC 1 cut(s) 621
PspPI GGNCC 5 cut(s) 460, 621, 622, 741, 1010
PstNI CAGNNNCTG 1 cut(s) 848
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 4 cut(s) 434, 566, 897, 1037
RsaNI GTAC 4 cut(s) 433, 565, 896, 1036
RseI CAYNNNNRTG 1 cut(s) 209
Sau3AI GATC 2 cut(s) 280, 631
Sau96I GGNCC 5 cut(s) 460, 621, 622, 741, 1010
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 7 cut(s) 83, 326, 449, 494, 630, 962, 1009
ScrFI CCNGG 4 cut(s) 490, 620, 745, 1007
SduI GDGCHC 4 cut(s) 354, 625, 691, 948
SfaNI GCATC 3 cut(s) 328, 336, 775
SfoI GGCGCC 1 cut(s) 813
SinI GGWCC 2 cut(s) 741, 1010
SmiMI CAYNNNNRTG 1 cut(s) 209
Sse9I AATT 6 cut(s) 64, 131, 250, 1031, 1054, 1076
SspDI GGCGCC 1 cut(s) 811
StyD4I CCNGG 4 cut(s) 488, 618, 743, 1005
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 2 cut(s) 800, 830
TaiI ACGT 4 cut(s) 387, 543, 570, 600
TasI AATT 6 cut(s) 64, 131, 250, 1031, 1054, 1076
TatI WGTACW 1 cut(s) 432
TauI GCSGC 6 cut(s) 316, 319, 450, 616, 660, 674
TfiI GAWTC 2 cut(s) 290, 409
TscAI CASTG 3 cut(s) 47, 64, 727
TseI GCWGC 8 cut(s) 150, 181, 418, 528, 839, 931, 938, 1046
TspDTI ATGAA 2 cut(s) 631, 878
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 3 cut(s) 47, 64, 727
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 741, 1010
XagI CCTNNNNNAGG 1 cut(s) 548
XapI RAATTY 1 cut(s) 1076
XceI RCATGY 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 434
ZraI GACGTC 2 cut(s) 385, 598
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.