pycom14g02910

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004708
Physical Location & Seq
Reverse (-)
2223445 .. 2224941
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02910.1

Sequence Viewer

Length: 1113 bp
ATGTCCATAAACCACTTCTCATCGGACCTCTCAGAAACCCAGTGGTGGAACACCTCCAGTGCTAATTCTAACCCCCACCAACAAGATGACTCCTCAGGACACCCCCACAGCAGAACCCATATCATCCACCAATTTTATCAAGATGGTGGCAGCCACCCCCATCACAGTTTCCAACCCTTGCAGCTCAACTCCCAAGACCCCCCCACCCATTTCCACACCCCTACCACCACCTTCAATTTCAACCTCAACCACCACGACGCCATCGATCCCAACGAATCAGACCAACTCCAGCCCCCGCAGCCGCATCCCCGCATCGACTCCTACGACGAGAAAGAACCCATGTTCGAGAAGCCCTTGACCCCCAGCGACGTCGGCAAGCTCAACCGCCTCGTGATTCCCAAGCAGCACGCGGAGAAGTACTTCCCGCTCGGCGGCGGAGACTCGGGCCTCCTGCTCAGTTTCGAGGACGAGTCCGGGAAGTCGTGGCGGTTCCGCTACTCCTACTGGAACAGCAGCCAAAGCTACGTCCTCACCAAGGGCTGGAGCCGGTACGTCAAGGAGAAGCGCCTCGACGCAGGCGACGTCGTTTTGTTCGAGCGGCTCCGGGCCCACACGGATCGACTCTTCATCGGCTGGAGACGCCGCAACGCGGTCCCGGCGCACGATAGCGGTGCTCAGGTTAGCAGAGGAGGGGTTGAGAGCAGCAGTGGTGGTGGCGGAGGTTGGACCAGGATGTTGTATTCTGCACCGCATCACGGCTCGTCTTATCCTGCGCACCAGCATCATCATAGTGTGGCGCCATACCCACCTGACTGTCTACATGCAGCAGGTGATGTATATCCTCTTAATTGGTCTGATGTTCAGAATATGAACCAGACGACATCGGTTGGGAACTCGAAGGTACTGAGGCTATTTGGGGTGAACTTGGAGTGCCAGCAGCAGGCAGCTGAGCACCACGAGTCGGAACCATCGACCCCAGATGGCTCATCATCGAGTTTGTCGATGTCGAGCCAGGGTCCGACTTACGGCCAGCAACAATTGTACGCTCCTGCTGCTTATAATTCTGCTGACCAACACAGGGTACGTAAACCCCCTAAACTATCATTGTCCTAA

Protein Analysis

371

Amino Acids

41.47

Weight (kDa)

6.44

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 115 - 216 1.9e-27 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AarI CACCTGC 1 cut(s) 818
AatII GACGTC 2 cut(s) 372, 585
Acc16I TGCGCA 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 818
AccB1I GGYRCC 1 cut(s) 796
AccB7I CCANNNNNTGG 1 cut(s) 540
AccBSI CCGCTC 2 cut(s) 427, 598
AccI GTMKAC 1 cut(s) 817
AccII CGCG 2 cut(s) 410, 650
AclWI GGATC 2 cut(s) 260, 624
AcoI YGGCCR 1 cut(s) 1027
AcyI GRCGYC 5 cut(s) 258, 369, 582, 640, 797
AfaI GTAC 5 cut(s) 419, 551, 903, 1043, 1083
AgsI TTSAA 2 cut(s) 235, 241
AjnI CCWGG 2 cut(s) 728, 1011
AluBI AGCT 4 cut(s) 184, 379, 522, 947
AluI AGCT 4 cut(s) 184, 379, 522, 947
Alw21I GWGCWC 2 cut(s) 676, 954
Alw26I GTCTC 2 cut(s) 432, 631
AlwI GGATC 2 cut(s) 260, 624
Ama87I CYCGRG 1 cut(s) 442
AoxI GGCC 3 cut(s) 445, 606, 1027
ApaI GGGCCC 1 cut(s) 610
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 4 cut(s) 567, 661, 775, 799
AspS9I GGNCC 7 cut(s) 25, 445, 606, 607, 652, 726, 1016
AsuC2I CCSGG 3 cut(s) 475, 605, 656
AsuHPI GGTGA 3 cut(s) 523, 842, 931
AvaI CYCGRG 1 cut(s) 442
AvaII GGWCC 4 cut(s) 25, 652, 726, 1016
AxyI CCTNAGG 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 610
BauI CACGAG 2 cut(s) 389, 956
Bbv12I GWGCWC 2 cut(s) 676, 954
BccI CCATC 5 cut(s) 137, 168, 269, 974, 976
BceAI ACGGC 2 cut(s) 772, 1042
BcgI CGANNNNNNTGC 2 cut(s) 653, 687
BciT130I CCWGG 2 cut(s) 730, 1013
BcnI CCSGG 3 cut(s) 475, 605, 656
BcoDI GTCTC 2 cut(s) 432, 631
BfoI RGCGCY 2 cut(s) 568, 800
BfuAI ACCTGC 1 cut(s) 818
BlpI GCTNAGC 1 cut(s) 948
BmcAI AGTACT 1 cut(s) 419
Bme1390I CCNGG 5 cut(s) 475, 605, 656, 730, 1013
Bme18I GGWCC 4 cut(s) 25, 652, 726, 1016
BmeT110I CYCGRG 1 cut(s) 442
BmgT120I GGNCC 7 cut(s) 25, 445, 606, 607, 652, 726, 1016
BmiI GGNNCC 8 cut(s) 491, 545, 602, 608, 654, 798, 966, 1017
BmrFI CCNGG 5 cut(s) 475, 605, 656, 730, 1013
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 4 cut(s) 313, 321, 760, 790
BmuI ACTGGG 1 cut(s) 34
BpmI CTGGAG 4 cut(s) 40, 272, 562, 655
Bpu10I CCTNAGC 1 cut(s) 675
Bpu1102I GCTNAGC 1 cut(s) 948
BpuMI CCSGG 3 cut(s) 475, 605, 656
Bsa29I ATCGAT 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 1085
BsaBI GATNNNNATC 1 cut(s) 837
BsaHI GRCGYC 5 cut(s) 258, 369, 582, 640, 797
BsaJI CCNNGG 2 cut(s) 534, 1012
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 3 cut(s) 40, 57, 509
Bse21I CCTNAGG 1 cut(s) 94
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 2 cut(s) 730, 1013
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 534, 1012
BseGI GGATG 3 cut(s) 123, 304, 738
BseJI GATNNNNATC 1 cut(s) 837
BseMII CTCAG 6 cut(s) 45, 108, 469, 689, 896, 939
BseNI ACTGG 3 cut(s) 40, 57, 509
BseRI GAGGAG 2 cut(s) 82, 702
BseSI GKGCMC 1 cut(s) 610
BseYI CCCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 2 cut(s) 410, 650
BshFI GGCC 3 cut(s) 447, 608, 1029
BshNI GGYRCC 1 cut(s) 796
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 676, 954
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 4 cut(s) 474, 547, 604, 656
BslFI GGGAC 1 cut(s) 638
BsmAI GTCTC 2 cut(s) 432, 631
BsmBI CGTCTC 1 cut(s) 631
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 3 cut(s) 447, 608, 1029
BsoBI CYCGRG 1 cut(s) 442
Bsp120I GGGCCC 1 cut(s) 606
Bsp1286I GDGCHC 3 cut(s) 610, 676, 954
Bsp143I GATC 2 cut(s) 265, 616
Bsp1720I GCTNAGC 1 cut(s) 948
BspANI GGCC 3 cut(s) 447, 608, 1029
BspCNI CTCAG 6 cut(s) 44, 107, 468, 688, 897, 940
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 2 cut(s) 410, 650
BspLI GGNNCC 8 cut(s) 491, 545, 602, 608, 654, 798, 966, 1017
BspMI ACCTGC 1 cut(s) 818
BspPI GGATC 2 cut(s) 260, 624
BspT107I GGYRCC 1 cut(s) 796
BsrBI CCGCTC 2 cut(s) 427, 598
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 3 cut(s) 40, 57, 509
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 534, 1012
BssMI GATC 2 cut(s) 265, 616
BssNI GRCGYC 5 cut(s) 258, 369, 582, 640, 797
BssSI CACGAG 2 cut(s) 389, 956
BssT1I CCWWGG 1 cut(s) 534
Bst2BI CACGAG 2 cut(s) 389, 956
Bst2UI CCWGG 2 cut(s) 730, 1013
Bst4CI ACNGT 2 cut(s) 167, 815
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 5 cut(s) 258, 369, 582, 640, 797
BstBAI YACGTR 1 cut(s) 1085
BstC8I GCNNGC 6 cut(s) 377, 408, 577, 935, 942, 1031
BstDEI CTNAG 6 cut(s) 31, 94, 455, 675, 905, 948
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 3 cut(s) 123, 304, 738
BstFNI CGCG 2 cut(s) 410, 650
BstH2I RGCGCY 2 cut(s) 568, 800
BstHHI GCGC 4 cut(s) 567, 661, 775, 799
BstKTI GATC 2 cut(s) 268, 619
BstMAI GTCTC 2 cut(s) 432, 631
BstMBI GATC 2 cut(s) 265, 616
BstMWI GCNNNNNNNGC 6 cut(s) 298, 372, 519, 639, 656, 1052
BstNI CCWGG 2 cut(s) 730, 1013
BstNSI RCATGY 1 cut(s) 824
BstSCI CCNGG 5 cut(s) 473, 603, 654, 728, 1011
BstSLI GKGCMC 1 cut(s) 610
BstSNI TACGTA 1 cut(s) 1085
BstUI CGCG 2 cut(s) 410, 650
Bsu15I ATCGAT 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 94
BsuRI GGCC 3 cut(s) 447, 608, 1029
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 3 cut(s) 123, 304, 738
BtsI GCAGTG 1 cut(s) 712
BtsIMutI CAGTG 3 cut(s) 47, 64, 712
BveI ACCTGC 1 cut(s) 818
Cac8I GCNNGC 6 cut(s) 377, 408, 577, 935, 942, 1031
CfoI GCGC 4 cut(s) 567, 661, 775, 799
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 7 cut(s) 25, 445, 606, 607, 652, 726, 1016
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 3 cut(s) 266, 581, 648
Csp6I GTAC 5 cut(s) 418, 550, 902, 1042, 1082
CviAII CATG 2 cut(s) 340, 821
CviQI GTAC 5 cut(s) 418, 550, 902, 1042, 1082
DdeI CTNAG 6 cut(s) 31, 94, 455, 675, 905, 948
DinI GGCGCC 1 cut(s) 798
DpnI GATC 2 cut(s) 267, 618
DpnII GATC 2 cut(s) 265, 616
EaeI YGGCCR 1 cut(s) 1027
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 2 cut(s) 450, 732
Eco105I TACGTA 1 cut(s) 1085
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 610
Eco47I GGWCC 4 cut(s) 25, 652, 726, 1016
Eco81I CCTNAGG 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoRII CCWGG 2 cut(s) 728, 1011
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 610
EgeI GGCGCC 1 cut(s) 798
EheI GGCGCC 1 cut(s) 798
ErhI CCWWGG 1 cut(s) 534
Esp3I CGTCTC 1 cut(s) 631
FaeI CATG 2 cut(s) 343, 824
FaiI YATR 9 cut(s) 8, 120, 341, 789, 802, 822, 838, 869, 1059
FaqI GGGAC 1 cut(s) 638
FatI CATG 2 cut(s) 339, 820
FauI CCCGC 3 cut(s) 303, 317, 432
FblI GTMKAC 1 cut(s) 817
FokI GGATG 3 cut(s) 110, 291, 745
FriOI GRGCYC 1 cut(s) 610
FspI TGCGCA 1 cut(s) 774
GlaI GCGC 4 cut(s) 566, 660, 774, 798
GsaI CCCAGC 1 cut(s) 366
GsuI CTGGAG 4 cut(s) 40, 272, 562, 655
HaeII RGCGCY 2 cut(s) 568, 800
HaeIII GGCC 3 cut(s) 447, 608, 1029
HapII CCGG 4 cut(s) 474, 547, 604, 656
HgaI GACGC 3 cut(s) 266, 581, 648
HhaI GCGC 4 cut(s) 567, 661, 775, 799
Hin1I GRCGYC 5 cut(s) 258, 369, 582, 640, 797
Hin1II CATG 2 cut(s) 343, 824
Hin6I GCGC 4 cut(s) 565, 659, 773, 797
HinP1I GCGC 4 cut(s) 565, 659, 773, 797
HinfI GANTC 8 cut(s) 89, 275, 317, 394, 440, 470, 621, 959
HpaII CCGG 4 cut(s) 474, 547, 604, 656
HphI GGTGA 3 cut(s) 523, 842, 931
Hpy166II GTNNAC 3 cut(s) 818, 922, 1088
Hpy188I TCNGA 7 cut(s) 25, 34, 280, 856, 864, 964, 1020
Hpy188III TCNNGA 4 cut(s) 96, 140, 346, 391
Hpy8I GTNNAC 3 cut(s) 818, 922, 1088
Hpy99I CGWCG 7 cut(s) 260, 329, 371, 374, 575, 584, 587
HpyAV CCTTC 2 cut(s) 241, 892
HpyCH4III ACNGT 2 cut(s) 167, 815
HpyCH4IV ACGT 5 cut(s) 369, 525, 552, 582, 1084
HpyCH4V TGCA 3 cut(s) 181, 746, 824
HpyF10VI GCNNNNNNNGC 6 cut(s) 298, 372, 519, 639, 656, 1052
HpyF3I CTNAG 6 cut(s) 31, 94, 455, 675, 905, 948
HpySE526I ACGT 5 cut(s) 369, 525, 552, 582, 1084
Hsp92I GRCGYC 5 cut(s) 258, 369, 582, 640, 797
Hsp92II CATG 2 cut(s) 343, 824
HspAI GCGC 4 cut(s) 565, 659, 773, 797
KasI GGCGCC 1 cut(s) 796
Kzo9I GATC 2 cut(s) 265, 616
LmnI GCTCC 3 cut(s) 543, 606, 1051
LweI GCATC 4 cut(s) 313, 321, 760, 790
MaeII ACGT 5 cut(s) 369, 525, 552, 582, 1084
MalI GATC 2 cut(s) 267, 618
MbiI CCGCTC 2 cut(s) 427, 598
MboI GATC 2 cut(s) 265, 616
MboII GAAGA 1 cut(s) 616
MfeI CAATTG 1 cut(s) 1037
MhlI GDGCHC 3 cut(s) 610, 676, 954
MluCI AATT 6 cut(s) 64, 131, 235, 847, 1037, 1060
Mly113I GGCGCC 1 cut(s) 797
MlyI GAGTC 6 cut(s) 83, 311, 434, 479, 615, 968
MmeI TCCRAC 4 cut(s) 196, 704, 942, 1043
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 1 cut(s) 846
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 4 cut(s) 474, 547, 604, 656
MspR9I CCNGG 5 cut(s) 475, 605, 656, 730, 1013
MunI CAATTG 1 cut(s) 1037
MvaI CCWGG 2 cut(s) 730, 1013
MvnI CGCG 2 cut(s) 410, 650
MwoI GCNNNNNNNGC 6 cut(s) 298, 372, 519, 639, 656, 1052
NarI GGCGCC 1 cut(s) 797
NciI CCSGG 3 cut(s) 475, 605, 656
NdeII GATC 2 cut(s) 265, 616
NlaIII CATG 2 cut(s) 343, 824
NlaIV GGNNCC 8 cut(s) 491, 545, 602, 608, 654, 798, 966, 1017
NmeAIII GCCGAG 1 cut(s) 408
NsbI TGCGCA 1 cut(s) 774
NspI RCATGY 1 cut(s) 824
PaqCI CACCTGC 1 cut(s) 818
PcsI WCGNNNNNNNCGW 8 cut(s) 261, 270, 321, 576, 579, 591, 968, 998
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 275, 394
PflMI CCANNNNNTGG 1 cut(s) 540
PfoI TCCNGGA 1 cut(s) 473
PleI GAGTC 6 cut(s) 83, 311, 434, 478, 615, 967
PluTI GGCGCC 1 cut(s) 800
PpsI GAGTC 6 cut(s) 83, 311, 434, 478, 615, 967
Ppu21I YACGTR 1 cut(s) 1085
PsiI TTATAA 1 cut(s) 1059
Psp6I CCWGG 2 cut(s) 728, 1011
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 2 cut(s) 728, 1011
PspN4I GGNNCC 8 cut(s) 491, 545, 602, 608, 654, 798, 966, 1017
PspOMI GGGCCC 1 cut(s) 606
PspPI GGNCC 7 cut(s) 25, 445, 606, 607, 652, 726, 1016
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 5 cut(s) 419, 551, 903, 1043, 1083
RsaNI GTAC 5 cut(s) 418, 550, 902, 1042, 1082
RseI CAYNNNNRTG 1 cut(s) 789
SaqAI TTAA 1 cut(s) 846
Sau3AI GATC 2 cut(s) 265, 616
Sau96I GGNCC 7 cut(s) 25, 445, 606, 607, 652, 726, 1016
ScaI AGTACT 1 cut(s) 419
SchI GAGTC 6 cut(s) 83, 311, 434, 479, 615, 968
ScrFI CCNGG 5 cut(s) 475, 605, 656, 730, 1013
SduI GDGCHC 3 cut(s) 610, 676, 954
SfaNI GCATC 4 cut(s) 313, 321, 760, 790
SfoI GGCGCC 1 cut(s) 798
SinI GGWCC 4 cut(s) 25, 652, 726, 1016
SmiMI CAYNNNNRTG 1 cut(s) 789
SnaBI TACGTA 1 cut(s) 1085
Sse9I AATT 6 cut(s) 64, 131, 235, 847, 1037, 1060
SspDI GGCGCC 1 cut(s) 796
StyD4I CCNGG 5 cut(s) 473, 603, 654, 728, 1011
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 167, 815
TaiI ACGT 5 cut(s) 372, 528, 555, 585, 1087
TasI AATT 6 cut(s) 64, 131, 235, 847, 1037, 1060
TatI WGTACW 1 cut(s) 417
TauI GCSGC 4 cut(s) 304, 435, 601, 645
TfiI GAWTC 2 cut(s) 275, 394
Tru1I TTAA 1 cut(s) 846
Tru9I TTAA 1 cut(s) 846
TscAI CASTG 3 cut(s) 47, 64, 712
TspDTI ATGAA 2 cut(s) 616, 884
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 3 cut(s) 47, 64, 712
Van91I CCANNNNNTGG 1 cut(s) 540
VpaK11BI GGWCC 4 cut(s) 25, 652, 726, 1016
XagI CCTNNNNNAGG 1 cut(s) 533
XceI RCATGY 1 cut(s) 824
XmiI GTMKAC 1 cut(s) 817
XmnI GAANNNNTTC 1 cut(s) 419
ZraI GACGTC 2 cut(s) 370, 583
ZrmI AGTACT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.