Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007313

Physical Location & Seq

Genomic Coordinates Forward (+)

3946025 .. 3951958

Gene Structure 5934 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1036800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGGCCAAAGCTCTAAACCTAATTCCCAGTTCAATTTTCCCGCCTTTTTTATCCCCCACAATACCCAAAAGAAAATTCTGTACTATTAGAGCTACTACAGAGTCCTCTGAGACCCCCCAAACTTCAGCTTCTGTTCAAACCAAACCAGAACCTTCATCGCCGCCGTTAACATTTGCTCCTCCGCCCAATTTCAAGCCGCCGGAGCCGAAGCGGTTCGGCGTCAGGCCTGACAAAGTTTGGGACGTCTTAGGGGCTGCTCTTGCCTTGTTCTTCCGGTTGGGGACTGGCGTTTTCGTTTCTGGATATTCTTTCTCTTTCGTTCCGAAAAATGATATTCCTTCGGATCAGTATGCTCTCGAGTTTAATGATTCTAAGGTCAAAGAGACTTCAAAAGTAGGCCCCCGTCCGGCGAAGCCTATTGAGATATATGAGTTTGAAGGGTGTCCATTCTGCCGGAAGGTTAGGGAAATTGTTGCAGTATTGGACCTCGATGTTCTATTTTATCCTTGTCCACAAGGTGGTCCAAACTTTCGTCGCAAGGTTGCTGAGATGGGCGGAAAACAGCAGTTCCCTTACATGGTGGATCCAAACACTGGAGTTTCAATGTATGAATCAGATGACATAATCAAGTACTTGGTTGGGAAATATGGTGATGGAACTGTTCCTCTTGCATTGTCACTTGGTTTGTTAACGACTTTGACTGCAGGCTTTGCAATGATTGGTCGTGCGGGAAAGGGTTCTCGCTATTCTCCATCAAAACTACCACCCAAACCACTTTTAGTATGGGCATACGAGGGATCTCCTTTTTGCAAACTTGTGCGTGAAGTACTTGTAGAGTTAGAACTGCCTCACATATATCGCAGCTGTGCTCGTGGCAGTCCAAAACGACAAATACTGTTTGAGAAAACCGGACGTTTTCAGGCTCCTTACTTAGAAGATCCAAATACGGGGGTGGAAATGTTCGAAAGTGCAGAAATTGTAGAATATCTAAAAGCAACGTATGCTCTACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GST_N_3

Protein Analysis

338

Amino Acids

37.34

Weight (kDa)

8.96

Isoelectric Point (pI)

49.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N_3	PF13417	143 - 217	8.5e-17	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	148 - 212	7.7e-06	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	264 - 335	2.3e-11	Glutathione S-transferase, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03880
fragaria_vesca	FvH4_6g18820 FvH4_6g18820 FvH4_6g18820 FvH4_6g18820 FvH4_6g18820 FvH4_6g18820 FvH4_6g18820
malus_domestica	MD12G1036800.v1.1 MD14G1023500.v1.1
prunus_persica	Prupe.7G109400_v2.0.a1 Prupe.7G109400_v2.0.a1 Prupe.7G109400_v2.0.a1
pyrus_communis	pycom12g02420 pycom14g02240
rosa_chinensis	RchiOBHm_Chr3g0473831
rosa_laevigata	RLG00000023980
rosa_multiflora	Rmu_sc0005561.1_g000004
rosa_roxburghii	Rroxscaffold_6G00407740
rosa_rugosa	Rorug03G0135800
rosa_samantha	Rh3AG186300 Rh3BG214900 Rh3CG211600 Rh3DG210700
rosa_wichuraiana	Rw3G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	246
AccB7I	CCANNNNNTGG	2 cut(s)	518, 593
AciI	CCGC	7 cut(s)	41, 161, 182, 197, 211, 555, 728
AclWI	GGATC	5 cut(s)	351, 578, 591, 805, 932
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	1 cut(s)	74
AcuI	CTGAAG	1 cut(s)	108
AcyI	GRCGYC	2 cut(s)	219, 243
AdeI	CACNNNGTG	1 cut(s)	518
AfaI	GTAC	3 cut(s)	82, 632, 828
AfiI	CCNNNNNNNGG	5 cut(s)	406, 518, 577, 593, 947
AgsI	TTSAA	6 cut(s)	33, 137, 193, 390, 437, 603
AjuI	GAANNNNNNNTTGG	2 cut(s)	59, 91
AluBI	AGCT	4 cut(s)	11, 92, 128, 864
AluI	AGCT	4 cut(s)	11, 92, 128, 864
Alw21I	GWGCWC	1 cut(s)	871
Alw26I	GTCTC	2 cut(s)	104, 377
AlwI	GGATC	5 cut(s)	351, 578, 591, 805, 932
AlwNI	CAGNNNCTG	1 cut(s)	131
Ama87I	CYCGRG	1 cut(s)	356
AoxI	GGCC	3 cut(s)	3, 224, 397
ApeKI	GCWGC	2 cut(s)	254, 861
ApoI	RAATTY	1 cut(s)	74
Asp700I	GAANNNNTTC	2 cut(s)	212, 736
AspS9I	GGNCC	3 cut(s)	398, 484, 521
AsuHPI	GGTGA	1 cut(s)	662
AsuII	TTCGAA	1 cut(s)	963
AvaI	CYCGRG	1 cut(s)	356
AvaII	GGWCC	2 cut(s)	484, 521
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	583
BauI	CACGAG	1 cut(s)	870
Bbv12I	GWGCWC	1 cut(s)	871
BbvI	GCAGC	2 cut(s)	241, 873
BccI	CCATC	3 cut(s)	544, 647, 760
BceAI	ACGGC	1 cut(s)	148
BcoDI	GTCTC	2 cut(s)	104, 377
BfmI	CTRYAG	2 cut(s)	96, 702
BisI	GCNGC	4 cut(s)	161, 197, 255, 862
BlsI	GCNGC	4 cut(s)	162, 198, 256, 863
BmcAI	AGTACT	2 cut(s)	632, 828
Bme18I	GGWCC	2 cut(s)	484, 521
BmeT110I	CYCGRG	1 cut(s)	356
BmgT120I	GGNCC	3 cut(s)	398, 484, 521
BmiI	GGNNCC	4 cut(s)	204, 400, 585, 924
BmrI	ACTGGG	1 cut(s)	21
BmuI	ACTGGG	1 cut(s)	21
BpmI	CTGGAG	1 cut(s)	615
Bpu14I	TTCGAA	1 cut(s)	963
BsaHI	GRCGYC	2 cut(s)	219, 243
BsaI	GGTCTC	1 cut(s)	104
BsaWI	WCCGGW	2 cut(s)	273, 908
BsaXI	ACNNNNNCTCC	2 cut(s)	160, 190
Bsc4I	CCNNNNNNNGG	5 cut(s)	406, 518, 577, 593, 947
Bse1I	ACTGG	3 cut(s)	27, 289, 598
Bse3DI	GCAATG	1 cut(s)	720
BseLI	CCNNNNNNNGG	5 cut(s)	406, 518, 577, 593, 947
BseMI	GCAATG	1 cut(s)	720
BseMII	CTCAG	2 cut(s)	99, 537
BseNI	ACTGG	3 cut(s)	27, 289, 598
BseRI	GAGGAG	1 cut(s)	168
BseXI	GCAGC	2 cut(s)	241, 873
BsgI	GTGCAG	1 cut(s)	990
BshFI	GGCC	3 cut(s)	5, 226, 399
BsiHKAI	GWGCWC	1 cut(s)	871
BsiHKCI	CYCGRG	1 cut(s)	356
BsiSI	CCGG	5 cut(s)	200, 274, 407, 454, 909
BslFI	GGGAC	2 cut(s)	254, 295
BslI	CCNNNNNNNGG	5 cut(s)	406, 518, 577, 593, 947
BsmAI	GTCTC	2 cut(s)	104, 377
BsmFI	GGGAC	2 cut(s)	254, 295
BsnI	GGCC	3 cut(s)	5, 226, 399
Bso31I	GGTCTC	1 cut(s)	104
BsoBI	CYCGRG	1 cut(s)	356
Bsp119I	TTCGAA	1 cut(s)	963
Bsp1286I	GDGCHC	1 cut(s)	871
Bsp143I	GATC	4 cut(s)	343, 583, 797, 937
BspACI	CCGC	7 cut(s)	41, 161, 182, 197, 211, 555, 728
BspANI	GGCC	3 cut(s)	5, 226, 399
BspCNI	CTCAG	2 cut(s)	100, 538
BspLI	GGNNCC	4 cut(s)	204, 400, 585, 924
BspMAI	CTGCAG	1 cut(s)	706
BspPI	GGATC	5 cut(s)	351, 578, 591, 805, 932
BspT104I	TTCGAA	1 cut(s)	963
BspTNI	GGTCTC	1 cut(s)	104
BsrDI	GCAATG	1 cut(s)	720
BsrI	ACTGG	3 cut(s)	27, 289, 598
BssMI	GATC	4 cut(s)	343, 583, 797, 937
BssNI	GRCGYC	2 cut(s)	219, 243
BssSI	CACGAG	1 cut(s)	870
Bst2BI	CACGAG	1 cut(s)	870
Bst4CI	ACNGT	2 cut(s)	661, 897
BstACI	GRCGYC	2 cut(s)	219, 243
BstAPI	GCANNNNNTGC	2 cut(s)	710, 1001
BstBI	TTCGAA	1 cut(s)	963
BstC8I	GCNNGC	1 cut(s)	706
BstDEI	CTNAG	5 cut(s)	108, 247, 372, 546, 931
BstKTI	GATC	4 cut(s)	346, 586, 800, 940
BstMAI	GTCTC	2 cut(s)	104, 377
BstMBI	GATC	4 cut(s)	343, 583, 797, 937
BstMWI	GCNNNNNNNGC	4 cut(s)	202, 260, 710, 1001
BstSFI	CTRYAG	2 cut(s)	96, 702
BstV1I	GCAGC	2 cut(s)	241, 873
BstX2I	RGATCY	3 cut(s)	583, 797, 937
BstYI	RGATCY	3 cut(s)	583, 797, 937
BsuRI	GGCC	3 cut(s)	5, 226, 399
BtgZI	GCGATG	1 cut(s)	141
BtsIMutI	CAGTG	1 cut(s)	591
Cac8I	GCNNGC	1 cut(s)	706
CaiI	CAGNNNCTG	1 cut(s)	131
Cfr13I	GGNCC	3 cut(s)	398, 484, 521
CseI	GACGC	1 cut(s)	208
Csp6I	GTAC	3 cut(s)	81, 631, 827
CviAII	CATG	1 cut(s)	577
CviQI	GTAC	3 cut(s)	81, 631, 827
DdeI	CTNAG	5 cut(s)	108, 247, 372, 546, 931
DpnI	GATC	4 cut(s)	345, 585, 799, 939
DpnII	GATC	4 cut(s)	343, 583, 797, 937
DraIII	CACNNNGTG	1 cut(s)	518
EaeI	YGGCCR	1 cut(s)	3
EciI	GGCGGA	2 cut(s)	171, 570
Eco147I	AGGCCT	1 cut(s)	226
Eco31I	GGTCTC	1 cut(s)	104
Eco47I	GGWCC	2 cut(s)	484, 521
Eco57I	CTGAAG	1 cut(s)	108
Eco88I	CYCGRG	1 cut(s)	356
EcoO109I	RGGNCCY	1 cut(s)	398
FaeI	CATG	1 cut(s)	580
FaqI	GGGAC	2 cut(s)	254, 295
FatI	CATG	1 cut(s)	576
FauI	CCCGC	2 cut(s)	48, 721
Fnu4HI	GCNGC	4 cut(s)	161, 197, 255, 862
Fsp4HI	GCNGC	4 cut(s)	161, 197, 255, 862
GluI	GCNGC	4 cut(s)	161, 197, 255, 862
GsuI	CTGGAG	1 cut(s)	615
HaeIII	GGCC	3 cut(s)	5, 226, 399
HapII	CCGG	5 cut(s)	200, 274, 407, 454, 909
HgaI	GACGC	1 cut(s)	208
Hin1I	GRCGYC	2 cut(s)	219, 243
Hin1II	CATG	1 cut(s)	580
HincII	GTYRAC	2 cut(s)	168, 690
HindII	GTYRAC	2 cut(s)	168, 690
HinfI	GANTC	3 cut(s)	101, 368, 611
HpaI	GTTAAC	2 cut(s)	168, 690
HpaII	CCGG	5 cut(s)	200, 274, 407, 454, 909
HphI	GGTGA	1 cut(s)	662
Hpy166II	GTNNAC	3 cut(s)	168, 512, 690
Hpy188I	TCNGA	4 cut(s)	109, 324, 343, 616
Hpy188III	TCNNGA	2 cut(s)	300, 356
Hpy8I	GTNNAC	3 cut(s)	168, 512, 690
Hpy99I	CGWCG	1 cut(s)	537
HpyAV	CCTTC	4 cut(s)	162, 348, 431, 451
HpyCH4III	ACNGT	2 cut(s)	661, 897
HpyCH4IV	ACGT	3 cut(s)	243, 913, 998
HpyCH4V	TGCA	6 cut(s)	476, 671, 704, 713, 810, 971
HpyF10VI	GCNNNNNNNGC	4 cut(s)	202, 260, 710, 1001
HpyF3I	CTNAG	5 cut(s)	108, 247, 372, 546, 931
HpySE526I	ACGT	3 cut(s)	243, 913, 998
Hsp92I	GRCGYC	2 cut(s)	219, 243
Hsp92II	CATG	1 cut(s)	580
KspAI	GTTAAC	2 cut(s)	168, 690
Kzo9I	GATC	4 cut(s)	343, 583, 797, 937
LmnI	GCTCC	3 cut(s)	181, 202, 928
Lsp1109I	GCAGC	2 cut(s)	241, 873
MaeII	ACGT	3 cut(s)	243, 913, 998
MaeIII	GTNAC	1 cut(s)	675
MalI	GATC	4 cut(s)	345, 585, 799, 939
MboI	GATC	4 cut(s)	343, 583, 797, 937
MboII	GAAGA	2 cut(s)	262, 947
MflI	RGATCY	3 cut(s)	583, 797, 937
MhlI	GDGCHC	1 cut(s)	871
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	21, 33, 74, 187, 468, 975
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	110
MnlI	CCTC	6 cut(s)	115, 189, 497, 675, 787, 858
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	2 cut(s)	212, 736
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	3 cut(s)	167, 363, 689
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	864
MspI	CCGG	5 cut(s)	200, 274, 407, 454, 909
MwoI	GCNNNNNNNGC	4 cut(s)	202, 260, 710, 1001
NdeII	GATC	4 cut(s)	343, 583, 797, 937
NlaIII	CATG	1 cut(s)	580
NlaIV	GGNNCC	4 cut(s)	204, 400, 585, 924
NmuCI	GTSAC	1 cut(s)	675
NspV	TTCGAA	1 cut(s)	963
PaeR7I	CTCGAG	1 cut(s)	356
PceI	AGGCCT	1 cut(s)	226
PdmI	GAANNNNTTC	2 cut(s)	212, 736
PfeI	GAWTC	2 cut(s)	368, 611
PflMI	CCANNNNNTGG	2 cut(s)	518, 593
PkrI	GCNGC	4 cut(s)	162, 198, 256, 863
PleI	GAGTC	1 cut(s)	109
PpsI	GAGTC	1 cut(s)	109
PspN4I	GGNNCC	4 cut(s)	204, 400, 585, 924
PspPI	GGNCC	3 cut(s)	398, 484, 521
PstI	CTGCAG	1 cut(s)	706
PstNI	CAGNNNCTG	1 cut(s)	131
PsuI	RGATCY	3 cut(s)	583, 797, 937
PvuII	CAGCTG	1 cut(s)	864
RsaI	GTAC	3 cut(s)	82, 632, 828
RsaNI	GTAC	3 cut(s)	81, 631, 827
SaqAI	TTAA	3 cut(s)	167, 363, 689
SatI	GCNGC	4 cut(s)	161, 197, 255, 862
Sau3AI	GATC	4 cut(s)	343, 583, 797, 937
Sau96I	GGNCC	3 cut(s)	398, 484, 521
ScaI	AGTACT	2 cut(s)	632, 828
SchI	GAGTC	1 cut(s)	110
SduI	GDGCHC	1 cut(s)	871
SfcI	CTRYAG	2 cut(s)	96, 702
Sfr274I	CTCGAG	1 cut(s)	356
SfuI	TTCGAA	1 cut(s)	963
SinI	GGWCC	2 cut(s)	484, 521
SlaI	CTCGAG	1 cut(s)	356
SmlI	CTYRAG	1 cut(s)	356
SmoI	CTYRAG	1 cut(s)	356
Sse9I	AATT	6 cut(s)	21, 33, 74, 187, 468, 975
SseBI	AGGCCT	1 cut(s)	226
SsiI	CCGC	7 cut(s)	41, 161, 182, 197, 211, 555, 728
StuI	AGGCCT	1 cut(s)	226
TaaI	ACNGT	2 cut(s)	661, 897
TaiI	ACGT	3 cut(s)	246, 916, 1001
TaqI	TCGA	3 cut(s)	357, 489, 963
TasI	AATT	6 cut(s)	21, 33, 74, 187, 468, 975
TatI	WGTACW	3 cut(s)	80, 630, 826
TauI	GCSGC	2 cut(s)	163, 199
TfiI	GAWTC	2 cut(s)	368, 611
Tru1I	TTAA	3 cut(s)	167, 363, 689
Tru9I	TTAA	3 cut(s)	167, 363, 689
TscAI	CASTG	1 cut(s)	598
TseFI	GTSAC	1 cut(s)	675
TseI	GCWGC	2 cut(s)	254, 861
Tsp45I	GTSAC	1 cut(s)	675
TspDTI	ATGAA	2 cut(s)	144, 624
TspRI	CASTG	1 cut(s)	598
Van91I	CCANNNNNTGG	2 cut(s)	518, 593
VpaK11BI	GGWCC	2 cut(s)	484, 521
XapI	RAATTY	1 cut(s)	74
XcmI	CCANNNNNNNNNTGG	1 cut(s)	780
XhoI	CTCGAG	1 cut(s)	356
XmnI	GAANNNNTTC	2 cut(s)	212, 736
ZraI	GACGTC	1 cut(s)	244
ZrmI	AGTACT	2 cut(s)	632, 828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.