Rorug03G0135800

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007313
Physical Location & Seq
Forward (+)
11165962 .. 11168354
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0135800.1

Sequence Viewer

Length: 648 bp
ATGCCGGTCAGAGCGGCAGAGACCTCCGCGCCTCCTCAGCATCATTCAGGTTCAAACTGCGGACAGACATCTCCCCCACCCTTCACGCTTTTAAGCGTTGGCCAGGGCTTCTCCGGTACTCAAAATGTTTCGAGTCTGCAGAAAGAGGAAGCTTGGAGAGTAAATGTTCGGATACAAGGGTGTGATCTTGAGAATGGTTATCTGTGTGGAACCATGGAAGCACTTAATGTTCCCATGGCTGACACACCAGTCGTCACCTTTTGGGAAGGGGAGATTGTTGACACCAAGAATTATACTTTCTTCACTGAGAAGTGGGAAGCAACACACGATGATGATATAAGGCACTGGACCAAATTTCCTTCTTTTTCTGCTCTTTTGAGCCGAGTGGAAGTTGATGGTGGCAAATCATTAGATCTCAGCAACTATAAATACATATTCATGAGATGGAAGGAGCAATACTTTGTGAATGTTGGCACAGACTGTGGTTTGACTATAGCCGGCTTTTACTATGTGTGTTTCTCATGTAGTGATGGCTCCATCAATGGCTTTTATTATGATCCTAATAGCAGCCCGTTCCAGAAGCTTGAGCTGAAATCCACAAATGAGGGAAGATTAGGATTCAGCTTTTCGTCCTACGAACTGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

215

Amino Acids

24.17

Weight (kDa)

4.98

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vac_ImportDeg PF09783 31 - 199 2.3e-51 Vacuolar import and degradation protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 14
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 14, 27, 60
AclWI GGATC 1 cut(s) 551
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 1 cut(s) 118
AgsI TTSAA 1 cut(s) 54
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 4 cut(s) 152, 583, 589, 624
AluI AGCT 4 cut(s) 152, 583, 589, 624
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 1 cut(s) 551
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 348
AsuHPI GGTGA 1 cut(s) 247
AvaII GGWCC 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 164, 197
BalI TGGCCA 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BbvCI CCTCAGC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 579
BccI CCATC 4 cut(s) 389, 438, 524, 545
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 14
BfmI CTRYAG 2 cut(s) 137, 492
BfuI GTATCC 1 cut(s) 165
BglII AGATCT 1 cut(s) 412
BisI GCNGC 2 cut(s) 15, 568
BlsI GCNGC 2 cut(s) 16, 569
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 211, 535
BmrFI CCNGG 1 cut(s) 104
BmsI GCATC 1 cut(s) 49
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 2 cut(s) 209, 605
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 3 cut(s) 103, 213, 234
BsaWI WCCGGW 1 cut(s) 113
Bse118I RCCGGY 2 cut(s) 4, 497
Bse1I ACTGG 2 cut(s) 248, 350
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 103, 213, 234
BseMII CTCAG 3 cut(s) 50, 297, 430
BseNI ACTGG 2 cut(s) 248, 350
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 102
BsiSI CCGG 3 cut(s) 5, 114, 498
BsmAI GTCTC 1 cut(s) 14
BsnI GGCC 1 cut(s) 102
Bso31I GGTCTC 1 cut(s) 14
Bsp143I GATC 3 cut(s) 184, 412, 556
Bsp19I CCATGG 2 cut(s) 213, 234
BspACI CCGC 3 cut(s) 14, 27, 60
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 3 cut(s) 49, 298, 429
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 211, 535
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 14
BsrFI RCCGGY 2 cut(s) 4, 497
BsrI ACTGG 2 cut(s) 248, 350
BssAI RCCGGY 2 cut(s) 4, 497
BssECI CCNNGG 3 cut(s) 103, 213, 234
BssMI GATC 3 cut(s) 184, 412, 556
BssT1I CCWWGG 2 cut(s) 213, 234
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 36, 306, 416
BstDSI CCRYGG 2 cut(s) 213, 234
BstFNI CGCG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 31
BstKTI GATC 3 cut(s) 187, 415, 559
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 3 cut(s) 184, 412, 556
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 2 cut(s) 137, 492
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuI GTATCC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 213, 234
BtsIMutI CAGTG 2 cut(s) 303, 343
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 438
CfoI GCGC 1 cut(s) 31
Cfr10I RCCGGY 2 cut(s) 4, 497
Cfr13I GGNCC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 117
CviAII CATG 4 cut(s) 214, 235, 439, 522
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 3 cut(s) 36, 306, 416
DpnI GATC 3 cut(s) 186, 414, 558
DpnII GATC 3 cut(s) 184, 412, 556
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 213, 234
Eco31I GGTCTC 1 cut(s) 14
Eco47I GGWCC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 213, 234
ErhI CCWWGG 2 cut(s) 213, 234
FaeI CATG 4 cut(s) 217, 238, 442, 525
FatI CATG 4 cut(s) 213, 234, 438, 521
Fnu4HI GCNGC 2 cut(s) 15, 568
Fsp4HI GCNGC 2 cut(s) 15, 568
GlaI GCGC 1 cut(s) 30
GluI GCNGC 2 cut(s) 15, 568
HaeIII GGCC 1 cut(s) 102
HapII CCGG 3 cut(s) 5, 114, 498
HhaI GCGC 1 cut(s) 31
Hin1II CATG 4 cut(s) 217, 238, 442, 525
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 150, 581
HinfI GANTC 2 cut(s) 133, 618
HpaII CCGG 3 cut(s) 5, 114, 498
HphI GGTGA 1 cut(s) 247
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 2 cut(s) 11, 171
Hpy188III TCNNGA 3 cut(s) 188, 439, 577
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 4 cut(s) 91, 260, 369, 442
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 3 cut(s) 36, 306, 416
Hsp92II CATG 4 cut(s) 217, 238, 442, 525
HspAI GCGC 1 cut(s) 29
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Kzo9I GATC 3 cut(s) 184, 412, 556
LmnI GCTCC 2 cut(s) 451, 539
LpnPI CCDG 9 cut(s) 18, 33, 89, 116, 127, 261, 331, 511, 590
Lsp1109I GCAGC 1 cut(s) 579
LweI GCATC 1 cut(s) 49
MaeIII GTNAC 1 cut(s) 253
MalI GATC 3 cut(s) 186, 414, 558
MbiI CCGCTC 1 cut(s) 14
MboI GATC 3 cut(s) 184, 412, 556
MboII GAAGA 2 cut(s) 292, 621
MflI RGATCY 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 2 cut(s) 289, 353
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 5 cut(s) 34, 42, 45, 139, 598
Mox20I TGGCCA 1 cut(s) 102
MroNI GCCGGC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 102
MseI TTAA 2 cut(s) 92, 225
MslI CAYNNNNRTG 2 cut(s) 330, 437
Msp20I TGGCCA 1 cut(s) 102
MspI CCGG 3 cut(s) 5, 114, 498
MspR9I CCNGG 1 cut(s) 104
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 37
NaeI GCCGGC 1 cut(s) 499
NcoI CCATGG 2 cut(s) 213, 234
NdeII GATC 3 cut(s) 184, 412, 556
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 4 cut(s) 217, 238, 442, 525
NlaIV GGNNCC 2 cut(s) 211, 535
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 253
PagI TCATGA 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 618
PkrI GCNGC 2 cut(s) 16, 569
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 211, 535
PspPI GGNCC 1 cut(s) 348
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 2 cut(s) 330, 437
SaqAI TTAA 2 cut(s) 92, 225
SatI GCNGC 2 cut(s) 15, 568
Sau3AI GATC 3 cut(s) 184, 412, 556
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 104
SetI ASST 7 cut(s) 26, 52, 154, 260, 585, 591, 626
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 2 cut(s) 137, 492
SinI GGWCC 1 cut(s) 348
SmiMI CAYNNNNRTG 2 cut(s) 330, 437
SmlI CTYRAG 2 cut(s) 188, 584
SmoI CTYRAG 2 cut(s) 188, 584
Sse9I AATT 2 cut(s) 289, 353
SsiI CCGC 3 cut(s) 14, 27, 60
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 2 cut(s) 213, 234
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 1 cut(s) 131
TasI AATT 2 cut(s) 289, 353
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 618
Tru1I TTAA 2 cut(s) 92, 225
Tru9I TTAA 2 cut(s) 92, 225
TscAI CASTG 2 cut(s) 310, 350
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 427
TspRI CASTG 2 cut(s) 310, 350
VpaK11BI GGWCC 1 cut(s) 348
XapI RAATTY 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.