Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005317

Physical Location & Seq

Genomic Coordinates Reverse (-)

4650199 .. 4655169

Gene Structure 4971 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1042100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGGCTATGCAAACTGTGTATTTCAAAGGACATGAAGGAATTGCCCCTAATCCCATAGGCCAGTTTTCATCAGCATTGTCAGGACCTTGGTGGAGTTCCTTGGGATCTCAACCGGTGTATGGTGAATCCTGTGGTCTGTCGAAACCTTCTACTAAGGAGCAGCCTAATACTGGGGACCATTTCAATGCCACTAAACTTGCCGGGCGGGGTACTGAACAAGGACTGGAAAAAGGGAACACAAATCAGTTCACTATCTTTCCTGGGGATGGACAGAAGTCCCAGGCAGCAATCTCTCTGCAATCATCGCCAGGAAAATATCATGGTCATTTTGAGCTCGGATTCAGCCAGCCAGTGATCTGTGCAAAATATCCATTTATGGATCAATGCTATGGACTCTTCTCAACCTATGGACCTCAAATTTCGGGACGCATTATGCTGCCACTGAACTTGACTGAGGATGAAGGACCAATCTATGTAAATGCTAAGCAGTACCATGGAATCATGCGTCGCCGACAATCCCGTGCGAAGGCTGTCATTGAGAACAGAGCAGCTAGACTGCGTAAGCCGTATATGCATGAATCGCGCCATCGTCATGCAATGCGCCGACCAAGGGGCTGCGGTGGTCGTTTTCTGAACACTAAGACTATGAACAATGGGGACAAGGGAACTGAAGGGAAGCAAGTTGGTGATGGGCAGCTGTTTAGGCTTTCGGGTTCTCAGAGTTCTGAAGTCCTGCAATCCGACAGTGGAACTTTAAACTCGTCAAAGGAAACGAATGGCAGCATTTCAAACATATCCGGGTCAGAGGTGACAAGTATGTACTCTAGGGGAGACCTTGATTGCTTTTCAATCAATCATCTCGGTCCCTCCCTGCACTCTCTCTCAGACATGAGAGATAGTGCGCGTGGTATGGTCATTCCCACGAAATGGGTTGCAGCAGGAGATAACTGCTGCAACCTCAATGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0008150 GO:0009566 GO:0009567 GO:0009889 GO:0010468 GO:0010556 GO:0016602 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031326 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0044798 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051704 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFB_NFYA

Protein Analysis

323

Amino Acids

35.11

Weight (kDa)

9.21

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFB_NFYA	PF02045	156 - 210	4.2e-25	CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05690 AT3G05690 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510
fragaria_vesca	FvH4_6g20510
malus_domestica	MD12G1042100.v1.1 MD14G1041300.v1.1
prunus_persica	Prupe.7G093100_v2.0.a1 Prupe.7G093100_v2.0.a1
pyrus_communis	pycom12g03680 pycom14g03510
rosa_chinensis	RchiOBHm_Chr3g0476401
rosa_laevigata	RLG00000023772
rosa_multiflora	Rmu_sc0000114.1_g000002
rosa_roxburghii	Rroxscaffold_6G00405310
rosa_rugosa	Rorug03G0154100 Rorug03G0154100 Rorug03G0154100 Rorug03G0154200
rosa_samantha	Rh3AG205300 Rh3BG236800 Rh3CG231300 Rh3DG231200
rosa_wichuraiana	Rw3G018650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	927
AccII	CGCG	2 cut(s)	583, 904
AciI	CCGC	2 cut(s)	205, 618
AclWI	GGATC	2 cut(s)	112, 387
AcsI	RAATTY	1 cut(s)	417
AcuI	CTGAAG	2 cut(s)	690, 747
AfaI	GTAC	3 cut(s)	211, 491, 821
AfiI	CCNNNNNNNGG	6 cut(s)	119, 170, 266, 526, 610, 927
AgeI	ACCGGT	1 cut(s)	112
AgsI	TTSAA	4 cut(s)	25, 184, 789, 849
AjnI	CCWGG	3 cut(s)	259, 279, 307
AluBI	AGCT	3 cut(s)	334, 551, 697
AluI	AGCT	3 cut(s)	334, 551, 697
Alw21I	GWGCWC	1 cut(s)	336
Alw26I	GTCTC	1 cut(s)	825
AlwI	GGATC	2 cut(s)	112, 387
AoxI	GGCC	1 cut(s)	58
ApeKI	GCWGC	9 cut(s)	160, 284, 436, 548, 615, 694, 780, 935, 951
ApoI	RAATTY	1 cut(s)	417
AsiGI	ACCGGT	1 cut(s)	112
AspLEI	GCGC	3 cut(s)	585, 603, 904
AspS9I	GGNCC	5 cut(s)	83, 175, 410, 464, 863
AsuC2I	CCSGG	2 cut(s)	202, 799
AsuHPI	GGTGA	3 cut(s)	134, 698, 820
AvaII	GGWCC	5 cut(s)	83, 175, 410, 464, 863
BanII	GRGCYC	1 cut(s)	336
Bbv12I	GWGCWC	1 cut(s)	336
BbvI	GCAGC	9 cut(s)	172, 296, 423, 560, 602, 706, 792, 938, 947
BccI	CCATC	3 cut(s)	260, 594, 683
BceAI	ACGGC	1 cut(s)	550
BciT130I	CCWGG	3 cut(s)	261, 281, 309
BcnI	CCSGG	2 cut(s)	202, 799
BcoDI	GTCTC	1 cut(s)	825
BfaI	CTAG	2 cut(s)	552, 825
BisI	GCNGC	9 cut(s)	161, 285, 437, 549, 616, 695, 781, 936, 952
BlpI	GCTNAGC	1 cut(s)	483
BlsI	GCNGC	9 cut(s)	162, 286, 438, 550, 617, 696, 782, 937, 953
Bme1390I	CCNGG	5 cut(s)	202, 261, 281, 309, 799
Bme18I	GGWCC	5 cut(s)	83, 175, 410, 464, 863
BmgT120I	GGNCC	5 cut(s)	83, 175, 410, 464, 863
BmiI	GGNNCC	2 cut(s)	176, 865
BmrFI	CCNGG	5 cut(s)	202, 261, 281, 309, 799
BmrI	ACTGGG	1 cut(s)	180
BmuI	ACTGGG	1 cut(s)	180
BoxI	GACNNNNGTC	1 cut(s)	274
Bpu1102I	GCTNAGC	1 cut(s)	483
BpuMI	CCSGG	2 cut(s)	202, 799
BsaI	GGTCTC	1 cut(s)	825
BsaJI	CCNNGG	6 cut(s)	86, 99, 260, 279, 493, 608
BsaWI	WCCGGW	1 cut(s)	112
Bsc4I	CCNNNNNNNGG	6 cut(s)	119, 170, 266, 526, 610, 927
Bse118I	RCCGGY	1 cut(s)	112
Bse1I	ACTGG	4 cut(s)	61, 175, 228, 350
Bse3DI	GCAATG	1 cut(s)	603
BseBI	CCWGG	3 cut(s)	261, 281, 309
BseDI	CCNNGG	6 cut(s)	86, 99, 260, 279, 493, 608
BseGI	GGATG	2 cut(s)	271, 463
BseLI	CCNNNNNNNGG	6 cut(s)	119, 170, 266, 526, 610, 927
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	3 cut(s)	444, 731, 897
BseNI	ACTGG	4 cut(s)	61, 175, 228, 350
BseXI	GCAGC	9 cut(s)	172, 296, 423, 560, 602, 706, 792, 938, 947
BsgI	GTGCAG	1 cut(s)	857
Bsh1236I	CGCG	2 cut(s)	583, 904
BshFI	GGCC	1 cut(s)	60
BshTI	ACCGGT	1 cut(s)	112
BsiHKAI	GWGCWC	1 cut(s)	336
BsiSI	CCGG	3 cut(s)	113, 201, 798
BslFI	GGGAC	5 cut(s)	188, 262, 438, 671, 849
BslI	CCNNNNNNNGG	6 cut(s)	119, 170, 266, 526, 610, 927
BsmAI	GTCTC	1 cut(s)	825
BsmFI	GGGAC	5 cut(s)	188, 262, 438, 671, 849
BsnI	GGCC	1 cut(s)	60
Bso31I	GGTCTC	1 cut(s)	825
Bsp1286I	GDGCHC	1 cut(s)	336
Bsp143I	GATC	3 cut(s)	104, 354, 379
Bsp1720I	GCTNAGC	1 cut(s)	483
Bsp19I	CCATGG	1 cut(s)	493
BspACI	CCGC	2 cut(s)	205, 618
BspANI	GGCC	1 cut(s)	60
BspCNI	CTCAG	3 cut(s)	445, 730, 896
BspFNI	CGCG	2 cut(s)	583, 904
BspLI	GGNNCC	2 cut(s)	176, 865
BspPI	GGATC	2 cut(s)	112, 387
BspTNI	GGTCTC	1 cut(s)	825
BsrDI	GCAATG	1 cut(s)	603
BsrFI	RCCGGY	1 cut(s)	112
BsrI	ACTGG	4 cut(s)	61, 175, 228, 350
BssAI	RCCGGY	1 cut(s)	112
BssECI	CCNNGG	6 cut(s)	86, 99, 260, 279, 493, 608
BssMI	GATC	3 cut(s)	104, 354, 379
BssT1I	CCWWGG	4 cut(s)	86, 99, 493, 608
Bst2UI	CCWGG	3 cut(s)	261, 281, 309
Bst4CI	ACNGT	2 cut(s)	16, 746
Bst6I	CTCTTC	1 cut(s)	401
BstC8I	GCNNGC	1 cut(s)	347
BstDEI	CTNAG	6 cut(s)	153, 453, 483, 639, 717, 883
BstDSI	CCRYGG	1 cut(s)	493
BstF5I	GGATG	2 cut(s)	271, 463
BstFNI	CGCG	2 cut(s)	583, 904
BstHHI	GCGC	3 cut(s)	585, 603, 904
BstKTI	GATC	3 cut(s)	107, 357, 382
BstMAI	GTCTC	1 cut(s)	825
BstMBI	GATC	3 cut(s)	104, 354, 379
BstMWI	GCNNNNNNNGC	4 cut(s)	304, 571, 580, 703
BstNI	CCWGG	3 cut(s)	261, 281, 309
BstPAI	GACNNNNGTC	1 cut(s)	274
BstSCI	CCNGG	5 cut(s)	200, 259, 279, 307, 797
BstUI	CGCG	2 cut(s)	583, 904
BstV1I	GCAGC	9 cut(s)	172, 296, 423, 560, 602, 706, 792, 938, 947
BstX2I	RGATCY	1 cut(s)	104
BstYI	RGATCY	1 cut(s)	104
BsuRI	GGCC	1 cut(s)	60
BtgI	CCRYGG	1 cut(s)	493
BtgZI	GCGATG	1 cut(s)	288
BtsCI	GGATG	2 cut(s)	271, 463
BtsIMutI	CAGTG	3 cut(s)	357, 440, 751
Cac8I	GCNNGC	1 cut(s)	347
CfoI	GCGC	3 cut(s)	585, 603, 904
Cfr10I	RCCGGY	1 cut(s)	112
Cfr13I	GGNCC	5 cut(s)	83, 175, 410, 464, 863
CseI	GACGC	2 cut(s)	435, 494
Csp6I	GTAC	3 cut(s)	210, 490, 820
CspAI	ACCGGT	1 cut(s)	112
CspCI	CAANNNNNGTGG	4 cut(s)	178, 213, 429, 464
CviAII	CATG	7 cut(s)	32, 320, 494, 502, 575, 593, 889
CviQI	GTAC	3 cut(s)	210, 490, 820
DdeI	CTNAG	6 cut(s)	153, 453, 483, 639, 717, 883
DpnI	GATC	3 cut(s)	106, 356, 381
DpnII	GATC	3 cut(s)	104, 354, 379
DraI	TTTAAA	1 cut(s)	756
Eam1104I	CTCTTC	1 cut(s)	401
EarI	CTCTTC	1 cut(s)	401
Ecl136II	GAGCTC	1 cut(s)	334
Eco130I	CCWWGG	4 cut(s)	86, 99, 493, 608
Eco24I	GRGCYC	1 cut(s)	336
Eco31I	GGTCTC	1 cut(s)	825
Eco47I	GGWCC	5 cut(s)	83, 175, 410, 464, 863
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	2 cut(s)	690, 747
EcoICRI	GAGCTC	1 cut(s)	334
EcoO109I	RGGNCCY	1 cut(s)	83
EcoRII	CCWGG	3 cut(s)	259, 279, 307
EcoT14I	CCWWGG	4 cut(s)	86, 99, 493, 608
EcoT22I	ATGCAT	1 cut(s)	576
EcoT38I	GRGCYC	1 cut(s)	336
ErhI	CCWWGG	4 cut(s)	86, 99, 493, 608
FaeI	CATG	7 cut(s)	35, 323, 497, 505, 578, 596, 892
FaqI	GGGAC	5 cut(s)	188, 262, 438, 671, 849
FatI	CATG	7 cut(s)	31, 319, 493, 501, 574, 592, 888
FauI	CCCGC	1 cut(s)	198
Fnu4HI	GCNGC	9 cut(s)	161, 285, 437, 549, 616, 695, 781, 936, 952
FokI	GGATG	2 cut(s)	278, 470
FriOI	GRGCYC	1 cut(s)	336
Fsp4HI	GCNGC	9 cut(s)	161, 285, 437, 549, 616, 695, 781, 936, 952
FspBI	CTAG	2 cut(s)	552, 825
GlaI	GCGC	3 cut(s)	584, 602, 903
GluI	GCNGC	9 cut(s)	161, 285, 437, 549, 616, 695, 781, 936, 952
HaeIII	GGCC	1 cut(s)	60
HapII	CCGG	3 cut(s)	113, 201, 798
HgaI	GACGC	2 cut(s)	435, 494
HhaI	GCGC	3 cut(s)	585, 603, 904
Hin1II	CATG	7 cut(s)	35, 323, 497, 505, 578, 596, 892
Hin6I	GCGC	3 cut(s)	583, 601, 902
HinP1I	GCGC	3 cut(s)	583, 601, 902
HinfI	GANTC	5 cut(s)	125, 339, 393, 498, 578
HpaII	CCGG	3 cut(s)	113, 201, 798
HphI	GGTGA	3 cut(s)	134, 698, 820
Hpy166II	GTNNAC	1 cut(s)	249
Hpy188I	TCNGA	7 cut(s)	338, 633, 720, 727, 742, 805, 886
Hpy188III	TCNNGA	2 cut(s)	81, 423
Hpy8I	GTNNAC	1 cut(s)	249
Hpy99I	CGWCG	1 cut(s)	510
HpyAV	CCTTC	5 cut(s)	29, 156, 455, 520, 665
HpyCH4III	ACNGT	2 cut(s)	16, 746
HpyCH4V	TGCA	9 cut(s)	10, 298, 362, 574, 596, 736, 874, 935, 954
HpyF10VI	GCNNNNNNNGC	4 cut(s)	304, 571, 580, 703
HpyF3I	CTNAG	6 cut(s)	153, 453, 483, 639, 717, 883
Hsp92II	CATG	7 cut(s)	35, 323, 497, 505, 578, 596, 892
HspAI	GCGC	3 cut(s)	583, 601, 902
Kzo9I	GATC	3 cut(s)	104, 354, 379
LmnI	GCTCC	1 cut(s)	157
Lsp1109I	GCAGC	9 cut(s)	172, 296, 423, 560, 602, 706, 792, 938, 947
MaeI	CTAG	2 cut(s)	552, 825
MaeIII	GTNAC	1 cut(s)	808
MalI	GATC	3 cut(s)	106, 356, 381
MboI	GATC	3 cut(s)	104, 354, 379
MboII	GAAGA	1 cut(s)	388
MflI	RGATCY	1 cut(s)	104
MhlI	GDGCHC	1 cut(s)	336
MluCI	AATT	2 cut(s)	39, 417
MlyI	GAGTC	1 cut(s)	387
MmeI	TCCRAC	1 cut(s)	765
MnlI	CCTC	5 cut(s)	423, 448, 799, 877, 968
Mph1103I	ATGCAT	1 cut(s)	576
MseI	TTAA	1 cut(s)	755
MslI	CAYNNNNRTG	2 cut(s)	183, 591
MspA1I	CMGCKG	1 cut(s)	697
MspI	CCGG	3 cut(s)	113, 201, 798
MspR9I	CCNGG	5 cut(s)	202, 261, 281, 309, 799
MvaI	CCWGG	3 cut(s)	261, 281, 309
MvnI	CGCG	2 cut(s)	583, 904
MwoI	GCNNNNNNNGC	4 cut(s)	304, 571, 580, 703
NciI	CCSGG	2 cut(s)	202, 799
NcoI	CCATGG	1 cut(s)	493
NdeII	GATC	3 cut(s)	104, 354, 379
NlaIII	CATG	7 cut(s)	35, 323, 497, 505, 578, 596, 892
NlaIV	GGNNCC	2 cut(s)	176, 865
NmuCI	GTSAC	1 cut(s)	808
NsiI	ATGCAT	1 cut(s)	576
PfeI	GAWTC	4 cut(s)	125, 339, 498, 578
PflMI	CCANNNNNTGG	1 cut(s)	927
PinAI	ACCGGT	1 cut(s)	112
PkrI	GCNGC	9 cut(s)	162, 286, 438, 550, 617, 696, 782, 937, 953
PleI	GAGTC	1 cut(s)	387
PpsI	GAGTC	1 cut(s)	387
PpuMI	RGGWCCY	1 cut(s)	83
PshAI	GACNNNNGTC	1 cut(s)	274
Psp124BI	GAGCTC	1 cut(s)	336
Psp5II	RGGWCCY	1 cut(s)	83
Psp6I	CCWGG	3 cut(s)	259, 279, 307
PspGI	CCWGG	3 cut(s)	259, 279, 307
PspN4I	GGNNCC	2 cut(s)	176, 865
PspPI	GGNCC	5 cut(s)	83, 175, 410, 464, 863
PspPPI	RGGWCCY	1 cut(s)	83
PsuI	RGATCY	1 cut(s)	104
PvuII	CAGCTG	1 cut(s)	697
RsaI	GTAC	3 cut(s)	211, 491, 821
RsaNI	GTAC	3 cut(s)	210, 490, 820
RseI	CAYNNNNRTG	2 cut(s)	183, 591
SacI	GAGCTC	1 cut(s)	336
SaqAI	TTAA	1 cut(s)	755
SatI	GCNGC	9 cut(s)	161, 285, 437, 549, 616, 695, 781, 936, 952
Sau3AI	GATC	3 cut(s)	104, 354, 379
Sau96I	GGNCC	5 cut(s)	83, 175, 410, 464, 863
SchI	GAGTC	1 cut(s)	387
ScrFI	CCNGG	5 cut(s)	202, 261, 281, 309, 799
SduI	GDGCHC	1 cut(s)	336
SinI	GGWCC	5 cut(s)	83, 175, 410, 464, 863
SmiMI	CAYNNNNRTG	2 cut(s)	183, 591
Sse9I	AATT	2 cut(s)	39, 417
SsiI	CCGC	2 cut(s)	205, 618
SspMI	CTAG	2 cut(s)	552, 825
SstI	GAGCTC	1 cut(s)	336
StyD4I	CCNGG	5 cut(s)	200, 259, 279, 307, 797
StyI	CCWWGG	4 cut(s)	86, 99, 493, 608
TaaI	ACNGT	2 cut(s)	16, 746
TaqI	TCGA	1 cut(s)	140
TaqII	GACCGA	1 cut(s)	851
TasI	AATT	2 cut(s)	39, 417
TatI	WGTACW	1 cut(s)	819
TfiI	GAWTC	4 cut(s)	125, 339, 498, 578
Tru1I	TTAA	1 cut(s)	755
Tru9I	TTAA	1 cut(s)	755
TscAI	CASTG	3 cut(s)	357, 447, 751
TseFI	GTSAC	1 cut(s)	808
TseI	GCWGC	9 cut(s)	160, 284, 436, 548, 615, 694, 780, 935, 951
Tsp45I	GTSAC	1 cut(s)	808
TspDTI	ATGAA	5 cut(s)	48, 57, 474, 591, 662
TspRI	CASTG	3 cut(s)	357, 447, 751
Van91I	CCANNNNNTGG	1 cut(s)	927
VpaK11BI	GGWCC	5 cut(s)	83, 175, 410, 464, 863
XapI	RAATTY	1 cut(s)	417
XspI	CTAG	2 cut(s)	552, 825
Zsp2I	ATGCAT	1 cut(s)	576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.