Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005317

Physical Location & Seq

Genomic Coordinates Reverse (-)

3873166 .. 3878129

Gene Structure 4964 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1041300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGGCTATGCAAACTGTGTATTTCAAAGAACATGAAGGAATTGCCCCTAATCCCATAGGGCAGTTTTCATCAGCATTGTCAGGGCCTTGGTGGAGTGCCTTTGGATCTCAACCGGTGTATGGGGAATCCTGTGGCCAGTCGAAACCTTTTACCAGGGAACAGCCCAATACCGGGCAGGGTACTGAACAAGGACTGGAAAAAGGGCACACAAATCTGTTCACTATCTTTCCTGGGGACGGTCAAAAGTCCCAGGCAGCAATTGCTCCGCCGGGAAAATACCGTGGTCATTTTGAGCTTGGATTCAGCCAGCCTATGATCTGTGCAAAATATCCATATATGGATCAATGCTATGGACTCTTCTCAGCTTATGGACCTCAAATCTCGGGACGCATTATGCTGCCGCTGAACTTGACTACGGATGAAGGACCAATCTATGTAAATGCCAAGCAGTACCATGGAATCATCCGTCGCCGACAATCCCGTGCCAAGGCTGTCATTGAGAACAGGGCCGCTAGACTCCGTAAGCCGTACATGCATGAATCTCGCCATCGCCATGCAATGCGCCGACCAAGGGGCTGTGGTGGTCGTTTTCTGAACACCAAGACTATGAACAATGGGAACAAGGGAACTGAAGGGAAGCAAGGTGGTGATGGGCAGCTGTTTAGGTTTTCTGGTTCTCAGAGTCCTGAAGTCCTGCAATCTGACAGTGGAACTTTAAACTCGTCAAAGGAAACAAATGGCAGCAGTTCAAACATATCCGGGTCAGAGGTGTCAAGTATGTACTCTAGGGGAGATCTTGATCGCTTTTCAATCAATCATCTCGGTCCCTCTCTGCACTCTCTGTCAAACATGATGGACAGTGCACGTGGTATGGTCATTCCGACCAAATGGGTTGCAGCAGGAGATAACCGCTGCAACCTCAAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0008150 GO:0009566 GO:0009567 GO:0009889 GO:0010468 GO:0010556 GO:0016602 GO:0019219 GO:0019222 GO:0019953 GO:0022414 GO:0031323 GO:0031326 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0044798 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051704 GO:0060255 GO:0065007 GO:0080090 GO:0090575 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFB_NFYA

Protein Analysis

310

Amino Acids

33.94

Weight (kDa)

9.64

Isoelectric Point (pI)

47.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFB_NFYA	PF02045	143 - 197	8.5e-25	CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005317)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05690 AT3G05690 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510 AT5G06510
fragaria_vesca	FvH4_6g20510
malus_domestica	MD12G1042100.v1.1 MD14G1041300.v1.1
prunus_persica	Prupe.7G093100_v2.0.a1 Prupe.7G093100_v2.0.a1
pyrus_communis	pycom12g03680 pycom14g03510
rosa_chinensis	RchiOBHm_Chr3g0476401
rosa_laevigata	RLG00000023772
rosa_multiflora	Rmu_sc0000114.1_g000002
rosa_roxburghii	Rroxscaffold_6G00405310
rosa_rugosa	Rorug03G0154100 Rorug03G0154100 Rorug03G0154100 Rorug03G0154200
rosa_samantha	Rh3AG205300 Rh3BG236800 Rh3CG231300 Rh3DG231200
rosa_wichuraiana	Rw3G018650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	266, 401, 510, 910
AclWI	GGATC	2 cut(s)	112, 348
AcoI	YGGCCR	1 cut(s)	133
AcuI	CTGAAG	2 cut(s)	651, 708
AcvI	CACGTG	1 cut(s)	866
AfaI	GTAC	4 cut(s)	181, 452, 530, 782
AfiI	CCNNNNNNNGG	6 cut(s)	119, 170, 171, 236, 487, 571
AgeI	ACCGGT	1 cut(s)	112
AgsI	TTSAA	3 cut(s)	25, 750, 810
AjnI	CCWGG	3 cut(s)	152, 229, 249
AluBI	AGCT	3 cut(s)	295, 365, 658
AluI	AGCT	3 cut(s)	295, 365, 658
Alw21I	GWGCWC	1 cut(s)	865
Alw44I	GTGCAC	1 cut(s)	861
AlwI	GGATC	2 cut(s)	112, 348
Ama87I	CYCGRG	1 cut(s)	382
AoxI	GGCC	3 cut(s)	83, 133, 507
ApaLI	GTGCAC	1 cut(s)	861
ApeKI	GCWGC	6 cut(s)	254, 397, 655, 741, 896, 912
AsiGI	ACCGGT	1 cut(s)	112
AspLEI	GCGC	1 cut(s)	564
AspS9I	GGNCC	5 cut(s)	83, 371, 425, 507, 824
AsuC2I	CCSGG	3 cut(s)	172, 270, 760
AsuHPI	GGTGA	1 cut(s)	659
AvaI	CYCGRG	1 cut(s)	382
AvaII	GGWCC	3 cut(s)	371, 425, 824
BaeGI	GKGCMC	2 cut(s)	207, 865
BalI	TGGCCA	1 cut(s)	135
BbrPI	CACGTG	1 cut(s)	866
Bbv12I	GWGCWC	1 cut(s)	865
BbvI	GCAGC	6 cut(s)	266, 384, 667, 753, 899, 908
BccI	CCATC	3 cut(s)	555, 644, 847
BceAI	ACGGC	1 cut(s)	511
BcgI	CGANNNNNNTGC	2 cut(s)	524, 558
BciT130I	CCWGG	3 cut(s)	154, 231, 251
BcnI	CCSGG	3 cut(s)	172, 270, 760
BfaI	CTAG	2 cut(s)	513, 786
BglII	AGATCT	1 cut(s)	793
BisI	GCNGC	8 cut(s)	255, 398, 401, 510, 656, 742, 897, 913
BlsI	GCNGC	8 cut(s)	256, 399, 402, 511, 657, 743, 898, 914
Bme1390I	CCNGG	6 cut(s)	154, 172, 231, 251, 270, 760
Bme18I	GGWCC	3 cut(s)	371, 425, 824
BmeT110I	CYCGRG	1 cut(s)	382
BmgT120I	GGNCC	5 cut(s)	83, 371, 425, 507, 824
BmiI	GGNNCC	1 cut(s)	826
BmrFI	CCNGG	6 cut(s)	154, 172, 231, 251, 270, 760
BpuMI	CCSGG	3 cut(s)	172, 270, 760
BsaAI	YACGTR	1 cut(s)	866
BsaBI	GATNNNNATC	1 cut(s)	798
BsaJI	CCNNGG	8 cut(s)	86, 153, 230, 249, 280, 454, 486, 569
BsaWI	WCCGGW	1 cut(s)	112
Bsc4I	CCNNNNNNNGG	6 cut(s)	119, 170, 171, 236, 487, 571
Bse118I	RCCGGY	1 cut(s)	112
Bse1I	ACTGG	2 cut(s)	136, 198
Bse3DI	GCAATG	1 cut(s)	564
Bse8I	GATNNNNATC	1 cut(s)	798
BseBI	CCWGG	3 cut(s)	154, 231, 251
BseDI	CCNNGG	8 cut(s)	86, 153, 230, 249, 280, 454, 486, 569
BseGI	GGATG	2 cut(s)	424, 462
BseJI	GATNNNNATC	1 cut(s)	798
BseLI	CCNNNNNNNGG	6 cut(s)	119, 170, 171, 236, 487, 571
BseMI	GCAATG	1 cut(s)	564
BseMII	CTCAG	2 cut(s)	375, 692
BseNI	ACTGG	2 cut(s)	136, 198
BseSI	GKGCMC	2 cut(s)	207, 865
BseXI	GCAGC	6 cut(s)	266, 384, 667, 753, 899, 908
BsgI	GTGCAG	1 cut(s)	818
BshFI	GGCC	3 cut(s)	85, 135, 509
BshTI	ACCGGT	1 cut(s)	112
BsiHKAI	GWGCWC	1 cut(s)	865
BsiHKCI	CYCGRG	1 cut(s)	382
BsiSI	CCGG	4 cut(s)	113, 171, 269, 759
BslFI	GGGAC	4 cut(s)	232, 248, 399, 810
BslI	CCNNNNNNNGG	6 cut(s)	119, 170, 171, 236, 487, 571
BsmFI	GGGAC	4 cut(s)	232, 248, 399, 810
BsnI	GGCC	3 cut(s)	85, 135, 509
BsoBI	CYCGRG	1 cut(s)	382
Bsp1286I	GDGCHC	2 cut(s)	207, 865
Bsp143I	GATC	5 cut(s)	104, 315, 340, 793, 799
Bsp19I	CCATGG	1 cut(s)	454
BspACI	CCGC	4 cut(s)	266, 401, 510, 910
BspANI	GGCC	3 cut(s)	85, 135, 509
BspCNI	CTCAG	2 cut(s)	374, 691
BspLI	GGNNCC	1 cut(s)	826
BspPI	GGATC	2 cut(s)	112, 348
BsrDI	GCAATG	1 cut(s)	564
BsrFI	RCCGGY	1 cut(s)	112
BsrI	ACTGG	2 cut(s)	136, 198
BssAI	RCCGGY	1 cut(s)	112
BssECI	CCNNGG	8 cut(s)	86, 153, 230, 249, 280, 454, 486, 569
BssMI	GATC	5 cut(s)	104, 315, 340, 793, 799
BssT1I	CCWWGG	4 cut(s)	86, 454, 486, 569
Bst2UI	CCWGG	3 cut(s)	154, 231, 251
Bst4CI	ACNGT	5 cut(s)	16, 239, 281, 707, 860
Bst6I	CTCTTC	1 cut(s)	362
BstAPI	GCANNNNNTGC	1 cut(s)	260
BstBAI	YACGTR	1 cut(s)	866
BstC8I	GCNNGC	1 cut(s)	308
BstDEI	CTNAG	2 cut(s)	361, 678
BstDSI	CCRYGG	2 cut(s)	280, 454
BstF5I	GGATG	2 cut(s)	424, 462
BstHHI	GCGC	1 cut(s)	564
BstKTI	GATC	5 cut(s)	107, 318, 343, 796, 802
BstMBI	GATC	5 cut(s)	104, 315, 340, 793, 799
BstMWI	GCNNNNNNNGC	2 cut(s)	260, 532
BstNI	CCWGG	3 cut(s)	154, 231, 251
BstNSI	RCATGY	1 cut(s)	535
BstSCI	CCNGG	6 cut(s)	152, 170, 229, 249, 268, 758
BstSLI	GKGCMC	2 cut(s)	207, 865
BstV1I	GCAGC	6 cut(s)	266, 384, 667, 753, 899, 908
BstX2I	RGATCY	2 cut(s)	104, 793
BstYI	RGATCY	2 cut(s)	104, 793
BsuRI	GGCC	3 cut(s)	85, 135, 509
BtgI	CCRYGG	2 cut(s)	280, 454
BtgZI	GCGATG	1 cut(s)	533
BtsCI	GGATG	2 cut(s)	424, 462
BtsIMutI	CAGTG	2 cut(s)	712, 865
Cac8I	GCNNGC	1 cut(s)	308
CfoI	GCGC	1 cut(s)	564
Cfr10I	RCCGGY	1 cut(s)	112
Cfr13I	GGNCC	5 cut(s)	83, 371, 425, 507, 824
CseI	GACGC	1 cut(s)	396
Csp6I	GTAC	4 cut(s)	180, 451, 529, 781
CspAI	ACCGGT	1 cut(s)	112
CviAII	CATG	6 cut(s)	32, 455, 532, 536, 554, 850
CviQI	GTAC	4 cut(s)	180, 451, 529, 781
DdeI	CTNAG	2 cut(s)	361, 678
DpnI	GATC	5 cut(s)	106, 317, 342, 795, 801
DpnII	GATC	5 cut(s)	104, 315, 340, 793, 799
DraI	TTTAAA	1 cut(s)	717
EaeI	YGGCCR	1 cut(s)	133
Eam1104I	CTCTTC	1 cut(s)	362
EarI	CTCTTC	1 cut(s)	362
EciI	GGCGGA	1 cut(s)	255
Eco130I	CCWWGG	4 cut(s)	86, 454, 486, 569
Eco47I	GGWCC	3 cut(s)	371, 425, 824
Eco57I	CTGAAG	2 cut(s)	651, 708
Eco72I	CACGTG	1 cut(s)	866
Eco88I	CYCGRG	1 cut(s)	382
EcoO109I	RGGNCCY	1 cut(s)	83
EcoRII	CCWGG	3 cut(s)	152, 229, 249
EcoT14I	CCWWGG	4 cut(s)	86, 454, 486, 569
EcoT22I	ATGCAT	1 cut(s)	537
ErhI	CCWWGG	4 cut(s)	86, 454, 486, 569
FaeI	CATG	6 cut(s)	35, 458, 535, 539, 557, 853
FaqI	GGGAC	4 cut(s)	232, 248, 399, 810
FatI	CATG	6 cut(s)	31, 454, 531, 535, 553, 849
Fnu4HI	GCNGC	8 cut(s)	255, 398, 401, 510, 656, 742, 897, 913
FokI	GGATG	2 cut(s)	431, 449
Fsp4HI	GCNGC	8 cut(s)	255, 398, 401, 510, 656, 742, 897, 913
FspBI	CTAG	2 cut(s)	513, 786
GlaI	GCGC	1 cut(s)	563
GluI	GCNGC	8 cut(s)	255, 398, 401, 510, 656, 742, 897, 913
HaeIII	GGCC	3 cut(s)	85, 135, 509
HapII	CCGG	4 cut(s)	113, 171, 269, 759
HgaI	GACGC	1 cut(s)	396
HhaI	GCGC	1 cut(s)	564
Hin1II	CATG	6 cut(s)	35, 458, 535, 539, 557, 853
Hin6I	GCGC	1 cut(s)	562
HinP1I	GCGC	1 cut(s)	562
HinfI	GANTC	7 cut(s)	125, 300, 354, 459, 516, 539, 682
HpaII	CCGG	4 cut(s)	113, 171, 269, 759
HphI	GGTGA	1 cut(s)	659
Hpy166II	GTNNAC	2 cut(s)	219, 863
Hpy188I	TCNGA	5 cut(s)	594, 681, 703, 766, 882
Hpy188III	TCNNGA	3 cut(s)	384, 686, 797
Hpy8I	GTNNAC	2 cut(s)	219, 863
Hpy99I	CGWCG	1 cut(s)	471
HpyAV	CCTTC	3 cut(s)	29, 416, 626
HpyCH4III	ACNGT	5 cut(s)	16, 239, 281, 707, 860
HpyCH4IV	ACGT	1 cut(s)	865
HpyCH4V	TGCA	9 cut(s)	10, 323, 535, 557, 697, 835, 863, 896, 915
HpyF10VI	GCNNNNNNNGC	2 cut(s)	260, 532
HpyF3I	CTNAG	2 cut(s)	361, 678
HpySE526I	ACGT	1 cut(s)	865
Hsp92II	CATG	6 cut(s)	35, 458, 535, 539, 557, 853
HspAI	GCGC	1 cut(s)	562
Kzo9I	GATC	5 cut(s)	104, 315, 340, 793, 799
LmnI	GCTCC	1 cut(s)	268
Lsp1109I	GCAGC	6 cut(s)	266, 384, 667, 753, 899, 908
MaeI	CTAG	2 cut(s)	513, 786
MaeII	ACGT	1 cut(s)	865
MalI	GATC	5 cut(s)	106, 317, 342, 795, 801
MboI	GATC	5 cut(s)	104, 315, 340, 793, 799
MboII	GAAGA	1 cut(s)	349
MfeI	CAATTG	1 cut(s)	258
MflI	RGATCY	2 cut(s)	104, 793
MhlI	GDGCHC	2 cut(s)	207, 865
MlsI	TGGCCA	1 cut(s)	135
MluCI	AATT	2 cut(s)	39, 258
MluNI	TGGCCA	1 cut(s)	135
MlyI	GAGTC	3 cut(s)	348, 510, 691
MmeI	TCCRAC	1 cut(s)	905
MnlI	CCTC	4 cut(s)	384, 760, 838, 929
Mox20I	TGGCCA	1 cut(s)	135
Mph1103I	ATGCAT	1 cut(s)	537
MscI	TGGCCA	1 cut(s)	135
MseI	TTAA	1 cut(s)	716
MslI	CAYNNNNRTG	1 cut(s)	552
Msp20I	TGGCCA	1 cut(s)	135
MspA1I	CMGCKG	3 cut(s)	403, 658, 912
MspI	CCGG	4 cut(s)	113, 171, 269, 759
MspR9I	CCNGG	6 cut(s)	154, 172, 231, 251, 270, 760
MunI	CAATTG	1 cut(s)	258
MvaI	CCWGG	3 cut(s)	154, 231, 251
MwoI	GCNNNNNNNGC	2 cut(s)	260, 532
NciI	CCSGG	3 cut(s)	172, 270, 760
NcoI	CCATGG	1 cut(s)	454
NdeII	GATC	5 cut(s)	104, 315, 340, 793, 799
NlaIII	CATG	6 cut(s)	35, 458, 535, 539, 557, 853
NlaIV	GGNNCC	1 cut(s)	826
NsiI	ATGCAT	1 cut(s)	537
NspI	RCATGY	1 cut(s)	535
PfeI	GAWTC	4 cut(s)	125, 300, 459, 539
PinAI	ACCGGT	1 cut(s)	112
PkrI	GCNGC	8 cut(s)	256, 399, 402, 511, 657, 743, 898, 914
PleI	GAGTC	3 cut(s)	348, 510, 690
PmaCI	CACGTG	1 cut(s)	866
PmlI	CACGTG	1 cut(s)	866
PpsI	GAGTC	3 cut(s)	348, 510, 690
Ppu21I	YACGTR	1 cut(s)	866
Psp6I	CCWGG	3 cut(s)	152, 229, 249
PspCI	CACGTG	1 cut(s)	866
PspGI	CCWGG	3 cut(s)	152, 229, 249
PspN4I	GGNNCC	1 cut(s)	826
PspPI	GGNCC	5 cut(s)	83, 371, 425, 507, 824
PsuI	RGATCY	2 cut(s)	104, 793
PvuII	CAGCTG	1 cut(s)	658
RsaI	GTAC	4 cut(s)	181, 452, 530, 782
RsaNI	GTAC	4 cut(s)	180, 451, 529, 781
RseI	CAYNNNNRTG	1 cut(s)	552
SaqAI	TTAA	1 cut(s)	716
SatI	GCNGC	8 cut(s)	255, 398, 401, 510, 656, 742, 897, 913
Sau3AI	GATC	5 cut(s)	104, 315, 340, 793, 799
Sau96I	GGNCC	5 cut(s)	83, 371, 425, 507, 824
SchI	GAGTC	3 cut(s)	348, 510, 691
ScrFI	CCNGG	6 cut(s)	154, 172, 231, 251, 270, 760
SduI	GDGCHC	2 cut(s)	207, 865
SinI	GGWCC	3 cut(s)	371, 425, 824
SmiMI	CAYNNNNRTG	1 cut(s)	552
Sse9I	AATT	2 cut(s)	39, 258
SsiI	CCGC	4 cut(s)	266, 401, 510, 910
SspMI	CTAG	2 cut(s)	513, 786
StyD4I	CCNGG	6 cut(s)	152, 170, 229, 249, 268, 758
StyI	CCWWGG	4 cut(s)	86, 454, 486, 569
TaaI	ACNGT	5 cut(s)	16, 239, 281, 707, 860
TaiI	ACGT	1 cut(s)	868
TaqI	TCGA	1 cut(s)	140
TaqII	GACCGA	1 cut(s)	812
TasI	AATT	2 cut(s)	39, 258
TatI	WGTACW	1 cut(s)	780
TauI	GCSGC	2 cut(s)	403, 512
TfiI	GAWTC	4 cut(s)	125, 300, 459, 539
Tru1I	TTAA	1 cut(s)	716
Tru9I	TTAA	1 cut(s)	716
TscAI	CASTG	2 cut(s)	712, 865
TseI	GCWGC	6 cut(s)	254, 397, 655, 741, 896, 912
TspDTI	ATGAA	5 cut(s)	48, 57, 435, 552, 623
TspGWI	ACGGA	3 cut(s)	431, 455, 509
TspRI	CASTG	2 cut(s)	712, 865
VneI	GTGCAC	1 cut(s)	861
VpaK11BI	GGWCC	3 cut(s)	371, 425, 824
XceI	RCATGY	1 cut(s)	535
XspI	CTAG	2 cut(s)	513, 786
Zsp2I	ATGCAT	1 cut(s)	537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.