Rh3CG231300

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005317
Physical Location & Seq
Reverse (-)
21813261 .. 21816835
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG231300.1

Sequence Viewer

Length: 876 bp
ATGGCTATGCAAACTGTTTATTTCAAAGAACATGAAGGACTTGGGCAGTTGTCATCAGTGTTACCTTGGTGGGGTAGTGCCTTTGGACCTCAACAAGTTCATGGCGAATCCTGTGGCCAGTCAAAACCTTTTACCATGGAGCTTCCCAATAATGGAGACCAACCCACTGCCACTAAGCAACCCGGGCGGGGCACTGAACAAGGACTGGATAAAGGGAACACAAATCAGTTCACTATCTTCCCTGTGGATGGGAAAAAATCTCAGCCAGCGATCTCTCTGCAATCATCCCCACCAAAATATCGTGGTCATTTTGAGCTGGGATTCAGCCAACCTATGGGACGCATTATGCTGCCACTGAATTTGACTACAGATGAAGGACCAATTTATGTCAATGCTAAGCAATACCACGGAATCATCCGGCGCCGACAATCCCGTGCGAAGGCAGTCATGAAGAACAGAGCAGCTAGGCACCGTAAGCCCTATATGCATGAATCACGTCATCTTCATGCCATGCGCCGACCAAGGGGATGTGGTGGTCGTTTTCTGAATACCAAGACTTCGAACAATGGGAAAGGTAGAAATGAAATGAAGCTAGTTGGTGATGTGCAGCTGTTTAGGCACTCCGGTTCTCAGAGCTCTGAAGTCCTGCAATCTGAGAGTGGAACTTTGAACTCATCAAAAACAAATGGCAGCAGTTCCAACATATCAGGCTCAGAGGTGACCAGTACGTACTCGAGGGGAGATCCTTTTTCAATGAATCATCATCATCTCGGGCCCTCTGTGCATTATCTGTCAGACATGATGGATGGTCGTGGCATGGTTAAGCCCACAAAATGGATTGCAGCAGTGGATAACTGCTGCAACCTCAAAGTCTGA

Protein Analysis

291

Amino Acids

32.2

Weight (kDa)

9.92

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 127 - 181 1.9e-25 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 420, 468
AccB7I CCANNNNNTGG 2 cut(s) 334, 834
AciI CCGC 1 cut(s) 187
AclWI GGATC 1 cut(s) 737
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 1 cut(s) 660
AcyI GRCGYC 1 cut(s) 421
AfaI GTAC 2 cut(s) 727, 731
AfiI CCNNNNNNNGG 8 cut(s) 71, 152, 188, 248, 334, 439, 523, 834
AgsI TTSAA 3 cut(s) 25, 670, 753
AjiI CACGTC 1 cut(s) 497
AluBI AGCT 6 cut(s) 142, 316, 464, 592, 610, 636
AluI AGCT 6 cut(s) 142, 316, 464, 592, 610, 636
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 1 cut(s) 150
AlwI GGATC 1 cut(s) 737
Ama87I CYCGRG 3 cut(s) 182, 733, 770
AoxI GGCC 2 cut(s) 115, 773
ApaI GGGCCC 1 cut(s) 777
ApeKI GCWGC 6 cut(s) 349, 461, 607, 690, 842, 858
ApoI RAATTY 1 cut(s) 358
AspLEI GCGC 2 cut(s) 423, 516
AspS9I GGNCC 4 cut(s) 86, 377, 773, 774
AsuC2I CCSGG 2 cut(s) 183, 184
AsuHPI GGTGA 2 cut(s) 611, 730
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 3 cut(s) 182, 733, 770
AvaII GGWCC 2 cut(s) 86, 377
BaeGI GKGCMC 2 cut(s) 194, 777
BalI TGGCCA 1 cut(s) 117
BanI GGYRCC 2 cut(s) 420, 468
BanII GRGCYC 2 cut(s) 638, 777
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 6 cut(s) 336, 473, 619, 702, 845, 854
BccI CCATC 3 cut(s) 242, 796, 800
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BcnI CCSGG 2 cut(s) 183, 184
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 465, 593
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 424
BisI GCNGC 6 cut(s) 350, 462, 608, 691, 843, 859
BlpI GCTNAGC 1 cut(s) 396
BlsI GCNGC 6 cut(s) 351, 463, 609, 692, 844, 860
Bme1390I CCNGG 2 cut(s) 183, 184
Bme18I GGWCC 2 cut(s) 86, 377
BmeT110I CYCGRG 3 cut(s) 182, 733, 770
BmgBI CACGTC 1 cut(s) 497
BmgT120I GGNCC 4 cut(s) 86, 377, 773, 774
BmiI GGNNCC 3 cut(s) 422, 470, 775
BmrFI CCNGG 2 cut(s) 183, 184
Bpu1102I GCTNAGC 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 560
BpuMI CCSGG 2 cut(s) 183, 184
BsaAI YACGTR 1 cut(s) 729
BsaHI GRCGYC 1 cut(s) 421
BsaI GGTCTC 1 cut(s) 150
BsaJI CCNNGG 5 cut(s) 65, 135, 182, 406, 521
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 152, 188, 248, 334, 439, 523, 834
Bse1I ACTGG 3 cut(s) 118, 210, 723
BseDI CCNNGG 5 cut(s) 65, 135, 182, 406, 521
BseGI GGATG 5 cut(s) 253, 284, 414, 533, 811
BseLI CCNNNNNNNGG 8 cut(s) 71, 152, 188, 248, 334, 439, 523, 834
BseMII CTCAG 4 cut(s) 275, 644, 645, 726
BseNI ACTGG 3 cut(s) 118, 210, 723
BseSI GKGCMC 2 cut(s) 194, 777
BseXI GCAGC 6 cut(s) 336, 473, 619, 702, 845, 854
BseYI CCCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 626
BshFI GGCC 2 cut(s) 117, 775
BshNI GGYRCC 2 cut(s) 420, 468
BsiHKAI GWGCWC 1 cut(s) 638
BsiHKCI CYCGRG 3 cut(s) 182, 733, 770
BsiSI CCGG 3 cut(s) 183, 418, 624
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 8 cut(s) 71, 152, 188, 248, 334, 439, 523, 834
BsmAI GTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 2 cut(s) 117, 775
Bso31I GGTCTC 1 cut(s) 150
BsoBI CYCGRG 3 cut(s) 182, 733, 770
Bsp119I TTCGAA 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 773
Bsp1286I GDGCHC 3 cut(s) 194, 638, 777
Bsp143I GATC 2 cut(s) 270, 742
Bsp1720I GCTNAGC 1 cut(s) 396
Bsp19I CCATGG 1 cut(s) 135
BspACI CCGC 1 cut(s) 187
BspANI GGCC 2 cut(s) 117, 775
BspCNI CTCAG 4 cut(s) 274, 643, 646, 725
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 422, 470, 775
BspPI GGATC 1 cut(s) 737
BspT104I TTCGAA 1 cut(s) 560
BspT107I GGYRCC 2 cut(s) 420, 468
BspTNI GGTCTC 1 cut(s) 150
BsrI ACTGG 3 cut(s) 118, 210, 723
BssECI CCNNGG 5 cut(s) 65, 135, 182, 406, 521
BssMI GATC 2 cut(s) 270, 742
BssNI GRCGYC 1 cut(s) 421
BssT1I CCWWGG 3 cut(s) 65, 135, 521
Bst4CI ACNGT 2 cut(s) 16, 473
BstACI GRCGYC 1 cut(s) 421
BstBAI YACGTR 1 cut(s) 729
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 6 cut(s) 174, 261, 396, 630, 654, 712
BstDSI CCRYGG 2 cut(s) 135, 406
BstEII GGTNACC 1 cut(s) 718
BstF5I GGATG 5 cut(s) 253, 284, 414, 533, 811
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 2 cut(s) 423, 516
BstKTI GATC 2 cut(s) 273, 745
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 2 cut(s) 270, 742
BstMWI GCNNNNNNNGC 5 cut(s) 184, 475, 484, 616, 781
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 181, 182
BstSFI CTRYAG 1 cut(s) 366
BstSLI GKGCMC 2 cut(s) 194, 777
BstSNI TACGTA 1 cut(s) 729
BstV1I GCAGC 6 cut(s) 336, 473, 619, 702, 845, 854
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 2 cut(s) 117, 775
BtgI CCRYGG 2 cut(s) 135, 406
BtrI CACGTC 1 cut(s) 497
BtsCI GGATG 5 cut(s) 253, 284, 414, 533, 811
BtsI GCAGTG 2 cut(s) 165, 852
BtsIMutI CAGTG 5 cut(s) 63, 165, 192, 353, 852
Cac8I GCNNGC 1 cut(s) 267
CciI TCATGA 1 cut(s) 447
CfoI GCGC 2 cut(s) 423, 516
Cfr13I GGNCC 4 cut(s) 86, 377, 773, 774
Cfr9I CCCGGG 1 cut(s) 182
CseI GACGC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 726, 730
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 9 cut(s) 32, 101, 136, 448, 488, 506, 511, 799, 817
CviQI GTAC 2 cut(s) 726, 730
DdeI CTNAG 6 cut(s) 174, 261, 396, 630, 654, 712
DinI GGCGCC 1 cut(s) 422
DpnI GATC 2 cut(s) 272, 744
DpnII GATC 2 cut(s) 270, 742
EaeI YGGCCR 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 636
Eco105I TACGTA 1 cut(s) 729
Eco130I CCWWGG 3 cut(s) 65, 135, 521
Eco24I GRGCYC 2 cut(s) 638, 777
Eco31I GGTCTC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 86, 377
Eco53kI GAGCTC 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 660
Eco88I CYCGRG 3 cut(s) 182, 733, 770
Eco91I GGTNACC 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 718
EcoT14I CCWWGG 3 cut(s) 65, 135, 521
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 2 cut(s) 638, 777
EgeI GGCGCC 1 cut(s) 422
EheI GGCGCC 1 cut(s) 422
ErhI CCWWGG 3 cut(s) 65, 135, 521
FaeI CATG 9 cut(s) 35, 104, 139, 451, 491, 509, 514, 802, 820
FaqI GGGAC 1 cut(s) 351
FatI CATG 9 cut(s) 31, 100, 135, 447, 487, 505, 510, 798, 816
FauI CCCGC 1 cut(s) 180
Fnu4HI GCNGC 6 cut(s) 350, 462, 608, 691, 843, 859
FokI GGATG 5 cut(s) 260, 271, 401, 540, 818
FriOI GRGCYC 2 cut(s) 638, 777
Fsp4HI GCNGC 6 cut(s) 350, 462, 608, 691, 843, 859
FspBI CTAG 2 cut(s) 465, 593
GlaI GCGC 2 cut(s) 422, 515
GluI GCNGC 6 cut(s) 350, 462, 608, 691, 843, 859
GsaI CCCAGC 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 2 cut(s) 117, 775
HapII CCGG 3 cut(s) 183, 418, 624
HgaI GACGC 1 cut(s) 348
HhaI GCGC 2 cut(s) 423, 516
Hin1I GRCGYC 1 cut(s) 421
Hin1II CATG 9 cut(s) 35, 104, 139, 451, 491, 509, 514, 802, 820
Hin6I GCGC 2 cut(s) 421, 514
HinP1I GCGC 2 cut(s) 421, 514
HinfI GANTC 5 cut(s) 107, 321, 411, 491, 757
HpaII CCGG 3 cut(s) 183, 418, 624
HphI GGTGA 2 cut(s) 611, 730
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 7 cut(s) 546, 633, 640, 655, 715, 796, 875
Hpy188III TCNNGA 1 cut(s) 448
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 3 cut(s) 29, 368, 433
HpyCH4III ACNGT 2 cut(s) 16, 473
HpyCH4IV ACGT 2 cut(s) 496, 728
HpyCH4V TGCA 8 cut(s) 10, 280, 487, 607, 649, 784, 842, 861
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 475, 484, 616, 781
HpyF3I CTNAG 6 cut(s) 174, 261, 396, 630, 654, 712
HpySE526I ACGT 2 cut(s) 496, 728
Hsp92I GRCGYC 1 cut(s) 421
Hsp92II CATG 9 cut(s) 35, 104, 139, 451, 491, 509, 514, 802, 820
HspAI GCGC 2 cut(s) 421, 514
KasI GGCGCC 1 cut(s) 420
Kzo9I GATC 2 cut(s) 270, 742
LmnI GCTCC 1 cut(s) 139
Lsp1109I GCAGC 6 cut(s) 336, 473, 619, 702, 845, 854
MaeI CTAG 2 cut(s) 465, 593
MaeII ACGT 2 cut(s) 496, 728
MaeIII GTNAC 2 cut(s) 60, 718
MalI GATC 2 cut(s) 272, 744
MboI GATC 2 cut(s) 270, 742
MboII GAAGA 3 cut(s) 229, 463, 494
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 3 cut(s) 194, 638, 777
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 2 cut(s) 358, 381
MluNI TGGCCA 1 cut(s) 117
Mly113I GGCGCC 1 cut(s) 421
MmeI TCCRAC 1 cut(s) 723
MnlI CCTC 5 cut(s) 99, 709, 729, 787, 875
Mox20I TGGCCA 1 cut(s) 117
Mph1103I ATGCAT 1 cut(s) 489
MscI TGGCCA 1 cut(s) 117
MseI TTAA 1 cut(s) 822
MslI CAYNNNNRTG 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 610
MspI CCGG 3 cut(s) 183, 418, 624
MspR9I CCNGG 2 cut(s) 183, 184
MwoI GCNNNNNNNGC 5 cut(s) 184, 475, 484, 616, 781
NarI GGCGCC 1 cut(s) 421
NciI CCSGG 2 cut(s) 183, 184
NcoI CCATGG 1 cut(s) 135
NdeII GATC 2 cut(s) 270, 742
NlaIII CATG 9 cut(s) 35, 104, 139, 451, 491, 509, 514, 802, 820
NlaIV GGNNCC 3 cut(s) 422, 470, 775
NmuCI GTSAC 1 cut(s) 718
NsiI ATGCAT 1 cut(s) 489
NspV TTCGAA 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 733
PagI TCATGA 1 cut(s) 447
PfeI GAWTC 5 cut(s) 107, 321, 411, 491, 757
PflMI CCANNNNNTGG 2 cut(s) 334, 834
PkrI GCNGC 6 cut(s) 351, 463, 609, 692, 844, 860
PluTI GGCGCC 1 cut(s) 424
Ppu21I YACGTR 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 638
PspEI GGTNACC 1 cut(s) 718
PspFI CCCAGC 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 422, 470, 775
PspOMI GGGCCC 1 cut(s) 773
PspPI GGNCC 4 cut(s) 86, 377, 773, 774
PspXI VCTCGAGB 1 cut(s) 733
PsuI RGATCY 1 cut(s) 742
PvuII CAGCTG 1 cut(s) 610
RsaI GTAC 2 cut(s) 727, 731
RsaNI GTAC 2 cut(s) 726, 730
RseI CAYNNNNRTG 1 cut(s) 504
SacI GAGCTC 1 cut(s) 638
SaqAI TTAA 1 cut(s) 822
SatI GCNGC 6 cut(s) 350, 462, 608, 691, 843, 859
Sau3AI GATC 2 cut(s) 270, 742
Sau96I GGNCC 4 cut(s) 86, 377, 773, 774
ScrFI CCNGG 2 cut(s) 183, 184
SduI GDGCHC 3 cut(s) 194, 638, 777
SfcI CTRYAG 1 cut(s) 366
SfoI GGCGCC 1 cut(s) 422
Sfr274I CTCGAG 1 cut(s) 733
SfuI TTCGAA 1 cut(s) 560
SinI GGWCC 2 cut(s) 86, 377
SlaI CTCGAG 1 cut(s) 733
SmaI CCCGGG 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 504
SmlI CTYRAG 1 cut(s) 733
SmoI CTYRAG 1 cut(s) 733
SnaBI TACGTA 1 cut(s) 729
Sse9I AATT 2 cut(s) 358, 381
SsiI CCGC 1 cut(s) 187
SspDI GGCGCC 1 cut(s) 420
SspMI CTAG 2 cut(s) 465, 593
SstI GAGCTC 1 cut(s) 638
StyD4I CCNGG 2 cut(s) 181, 182
StyI CCWWGG 3 cut(s) 65, 135, 521
TaaI ACNGT 2 cut(s) 16, 473
TaiI ACGT 2 cut(s) 499, 731
TaqI TCGA 2 cut(s) 560, 734
TasI AATT 2 cut(s) 358, 381
TfiI GAWTC 5 cut(s) 107, 321, 411, 491, 757
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 5 cut(s) 63, 172, 199, 360, 852
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 6 cut(s) 349, 461, 607, 690, 842, 858
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 9 cut(s) 48, 89, 387, 464, 494, 504, 597, 602, 770
TspGWI ACGGA 1 cut(s) 423
TspMI CCCGGG 1 cut(s) 182
TspRI CASTG 5 cut(s) 63, 172, 199, 360, 852
Van91I CCANNNNNTGG 2 cut(s) 334, 834
VpaK11BI GGWCC 2 cut(s) 86, 377
XapI RAATTY 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 182
XspI CTAG 2 cut(s) 465, 593
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.