Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005835

Physical Location & Seq

Genomic Coordinates Reverse (-)

5372221 .. 5375491

Gene Structure 3271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1046800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGAAATTTGGAGACACTTACACCGAGTTTTTGCACAAGGAAAAGATACGATTGTCGGAGAATTGCTCGCATGTCGAGTACAAGCGGCTGAAGAATGTCCTCAAGACTTGTCAGAGTTGTAAGGCATTGCAGGAATCTTCAGCTACGGAAGGACAAGAAGGCGCTTCTAACCAAAATCAGCAATTGTGCCAATGCCAATCTTGCACTTTGTGCGACCAGGCATTTTTCACGGAGTTAATGAAGGAGGTTTCAGAAATAGATATATGCTTTAGTTTGAGAGTCAGACATCTCCTTCATCTCCATGTTGCTACCGGGTTGCAGAGATACTTTCTGCTGTTCCGTCAGTGCTTCATGAATGATCAACAAGCCATGGTAGAAAAAGGGCGGATGTTGATTGAGTATGTTACGATGAACGCAATTGCTATCAGAAAAATCCTTAAGAAATATGATAAAATACATAGCTCCGAAAATGGCAAGCTTTTCAAATCCAAGATGCGGGCTGAACATATTGAGATTTTGCAATCACCTTGGCTTATAGAATTGGGTGCATTTTATTTGAATCTTGATGTATTGGATGGTGACGAGCTTAATGAGGTTTCTGGTCGCTTTTCCTGTGATCTCTCTGGGACACCACCTACAATGACATTGATGCTTCCTGACTCCTTAAAGATGGAATATGATCTAACTTGTGCTGTATGCTTGGAAACTGTTTTCAATCCCTATGCTTTGAGCTGCGGCCACCTGTTCTGCAAGTCCTGTGCGTGCGCAGCTGCTTCTGTGTTTATCTTCCAAGGCCCTAAATCTGCAGCCCCGGATGCAAAGTGCCCAATATGCAGAGAGGCTGGAGTGTATGCTAAAGCAGTGCACATGTTGGAACTAGACCTGCTCCTGAAAACAAGGTGCAACAACCATTGGAAAAAGAGGTATAATGCAGAACGCGCAGAGATACTGAAGCAAACTAAGGAGTTCTGGGATACGCAAGCAAAATATGCAGGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

334

Amino Acids

38.05

Weight (kDa)

7.78

Isoelectric Point (pI)

48.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RING_XB3-XBAT31	PF24921	229 - 280	2e-07	E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX	PF13445	230 - 254	3.3e-06	RING-type zinc-finger
zf-RING_5	PF14634	230 - 279	7.3e-06	zinc-RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38920 AT2G38920 AT2G38920 AT2G38920 AT2G38920 AT2G38920
fragaria_vesca	FvH4_6g21121 FvH4_6g21121
malus_domestica	MD12G1046800.v1.1 MD14G1045700.v1.1
prunus_persica	Prupe.7G088600_v2.0.a1 Prupe.7G088600_v2.0.a1 Prupe.7G088600_v2.0.a1 Prupe.7G088600_v2.0.a1
pyrus_communis	pycom14g03840
rosa_chinensis	RchiOBHm_Chr3g0477541
rosa_laevigata	RLG00000023710
rosa_multiflora	Rmu_sc0000775.1_g000014 Rmu_sc0040889.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403850 Rroxscaffold_6G00404550
rosa_rugosa	Rorug03G0161900
rosa_samantha	Rh3AG211900 Rh3BG245700 Rh3CG239300 Rh3DG238800 Rh5AG304100
rosa_wichuraiana	Rw3G019200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	766
Acc36I	ACCTGC	1 cut(s)	891
AccII	CGCG	1 cut(s)	939
AciI	CCGC	4 cut(s)	85, 385, 496, 735
AcoI	YGGCCR	1 cut(s)	736
AcsI	RAATTY	1 cut(s)	5
AcuI	CTGAAG	3 cut(s)	110, 123, 971
AdeI	CACNNNGTG	1 cut(s)	210
AfaI	GTAC	1 cut(s)	80
AfiI	CCNNNNNNNGG	1 cut(s)	495
AflII	CTTAAG	1 cut(s)	437
AflIII	ACRYGT	1 cut(s)	867
AgsI	TTSAA	3 cut(s)	484, 559, 715
AjnI	CCWGG	1 cut(s)	216
AluBI	AGCT	6 cut(s)	143, 462, 478, 586, 732, 770
AluI	AGCT	6 cut(s)	143, 462, 478, 586, 732, 770
Alw21I	GWGCWC	1 cut(s)	867
Alw26I	GTCTC	1 cut(s)	6
Alw44I	GTGCAC	1 cut(s)	863
AoxI	GGCC	2 cut(s)	736, 793
ApaLI	GTGCAC	1 cut(s)	863
ApeKI	GCWGC	4 cut(s)	732, 767, 770, 806
ApoI	RAATTY	1 cut(s)	5
AspLEI	GCGC	3 cut(s)	164, 767, 941
AspS9I	GGNCC	1 cut(s)	794
AsuC2I	CCSGG	2 cut(s)	313, 812
AsuHPI	GGTGA	2 cut(s)	516, 590
BaeGI	GKGCMC	2 cut(s)	827, 867
BaeI	ACNNNNGTAYC	2 cut(s)	38, 71
Bbv12I	GWGCWC	1 cut(s)	867
BbvI	GCAGC	4 cut(s)	719, 757, 779, 818
BccI	CCATC	2 cut(s)	569, 664
BciT130I	CCWGG	1 cut(s)	218
BciVI	GTATCC	1 cut(s)	967
BclI	TGATCA	1 cut(s)	358
BcnI	CCSGG	2 cut(s)	313, 812
BcoDI	GTCTC	1 cut(s)	6
BfaI	CTAG	1 cut(s)	878
BfmI	CTRYAG	1 cut(s)	804
BfoI	RGCGCY	1 cut(s)	165
BfrI	CTTAAG	1 cut(s)	437
BfuAI	ACCTGC	1 cut(s)	891
BfuI	GTATCC	1 cut(s)	967
BisI	GCNGC	6 cut(s)	86, 733, 736, 768, 771, 807
BlsI	GCNGC	6 cut(s)	87, 734, 737, 769, 772, 808
Bme1390I	CCNGG	3 cut(s)	218, 313, 812
BmgT120I	GGNCC	1 cut(s)	794
BmrFI	CCNGG	3 cut(s)	218, 313, 812
BmsI	GCATC	3 cut(s)	483, 639, 805
BplI	GAGNNNNNCTC	2 cut(s)	50, 82
BpmI	CTGGAG	1 cut(s)	864
BpuEI	CTTGAG	1 cut(s)	86
BpuMI	CCSGG	2 cut(s)	313, 812
BsaJI	CCNNGG	4 cut(s)	369, 527, 790, 810
Bsc4I	CCNNNNNNNGG	1 cut(s)	495
Bse3DI	GCAATG	1 cut(s)	125
BseBI	CCWGG	1 cut(s)	218
BseDI	CCNNGG	4 cut(s)	369, 527, 790, 810
BseGI	GGATG	3 cut(s)	393, 580, 820
BseLI	CCNNNNNNNGG	1 cut(s)	495
BseMI	GCAATG	1 cut(s)	125
BseSI	GKGCMC	2 cut(s)	827, 867
BseXI	GCAGC	4 cut(s)	719, 757, 779, 818
Bsh1236I	CGCG	1 cut(s)	939
BshFI	GGCC	2 cut(s)	738, 795
BsiHKAI	GWGCWC	1 cut(s)	867
BsiSI	CCGG	2 cut(s)	312, 812
BslFI	GGGAC	1 cut(s)	640
BslI	CCNNNNNNNGG	1 cut(s)	495
BsmAI	GTCTC	1 cut(s)	6
BsmFI	GGGAC	1 cut(s)	640
BsnI	GGCC	2 cut(s)	738, 795
Bsp1286I	GDGCHC	2 cut(s)	827, 867
Bsp143I	GATC	3 cut(s)	358, 616, 679
Bsp19I	CCATGG	1 cut(s)	369
BspACI	CCGC	4 cut(s)	85, 385, 496, 735
BspANI	GGCC	2 cut(s)	738, 795
BspFNI	CGCG	1 cut(s)	939
BspHI	TCATGA	1 cut(s)	351
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	1 cut(s)	891
BspTI	CTTAAG	1 cut(s)	437
BsrDI	GCAATG	1 cut(s)	125
BssECI	CCNNGG	4 cut(s)	369, 527, 790, 810
BssMI	GATC	3 cut(s)	358, 616, 679
BssT1I	CCWWGG	3 cut(s)	369, 527, 790
Bst2UI	CCWGG	1 cut(s)	218
Bst4CI	ACNGT	1 cut(s)	709
BstAFI	CTTAAG	1 cut(s)	437
BstAPI	GCANNNNNTGC	2 cut(s)	210, 989
BstC8I	GCNNGC	5 cut(s)	68, 476, 498, 763, 981
BstDEI	CTNAG	1 cut(s)	960
BstDSI	CCRYGG	1 cut(s)	369
BstF5I	GGATG	3 cut(s)	393, 580, 820
BstFNI	CGCG	1 cut(s)	939
BstH2I	RGCGCY	1 cut(s)	165
BstHHI	GCGC	3 cut(s)	164, 767, 941
BstKTI	GATC	3 cut(s)	361, 619, 682
BstMAI	GTCTC	1 cut(s)	6
BstMBI	GATC	3 cut(s)	358, 616, 679
BstMWI	GCNNNNNNNGC	7 cut(s)	201, 210, 767, 815, 831, 938, 989
BstNI	CCWGG	1 cut(s)	218
BstNSI	RCATGY	2 cut(s)	74, 871
BstSCI	CCNGG	3 cut(s)	216, 311, 810
BstSFI	CTRYAG	1 cut(s)	804
BstSLI	GKGCMC	2 cut(s)	827, 867
BstUI	CGCG	1 cut(s)	939
BstV1I	GCAGC	4 cut(s)	719, 757, 779, 818
BsuI	GTATCC	1 cut(s)	967
BsuRI	GGCC	2 cut(s)	738, 795
BtgI	CCRYGG	1 cut(s)	369
BtsCI	GGATG	3 cut(s)	393, 580, 820
BtsI	GCAGTG	1 cut(s)	867
BtsIMutI	CAGTG	2 cut(s)	350, 867
BveI	ACCTGC	1 cut(s)	891
Cac8I	GCNNGC	5 cut(s)	68, 476, 498, 763, 981
CciI	TCATGA	1 cut(s)	351
CfoI	GCGC	3 cut(s)	164, 767, 941
Cfr13I	GGNCC	1 cut(s)	794
Csp6I	GTAC	1 cut(s)	79
CviAII	CATG	5 cut(s)	71, 302, 352, 370, 868
CviQI	GTAC	1 cut(s)	79
DdeI	CTNAG	1 cut(s)	960
DpnI	GATC	3 cut(s)	360, 618, 681
DpnII	GATC	3 cut(s)	358, 616, 679
DraIII	CACNNNGTG	1 cut(s)	210
EaeI	YGGCCR	1 cut(s)	736
EciI	GGCGGA	1 cut(s)	400
Eco130I	CCWWGG	3 cut(s)	369, 527, 790
Eco57I	CTGAAG	3 cut(s)	110, 123, 971
EcoO109I	RGGNCCY	1 cut(s)	794
EcoRII	CCWGG	1 cut(s)	216
EcoT14I	CCWWGG	3 cut(s)	369, 527, 790
ErhI	CCWWGG	3 cut(s)	369, 527, 790
FaeI	CATG	5 cut(s)	74, 305, 355, 373, 871
FaqI	GGGAC	1 cut(s)	640
FatI	CATG	5 cut(s)	70, 301, 351, 369, 867
FauI	CCCGC	1 cut(s)	489
FbaI	TGATCA	1 cut(s)	358
Fnu4HI	GCNGC	6 cut(s)	86, 733, 736, 768, 771, 807
FokI	GGATG	3 cut(s)	400, 587, 827
Fsp4HI	GCNGC	6 cut(s)	86, 733, 736, 768, 771, 807
FspBI	CTAG	1 cut(s)	878
FspI	TGCGCA	1 cut(s)	766
GlaI	GCGC	3 cut(s)	163, 766, 940
GluI	GCNGC	6 cut(s)	86, 733, 736, 768, 771, 807
GsuI	CTGGAG	1 cut(s)	864
HaeII	RGCGCY	1 cut(s)	165
HaeIII	GGCC	2 cut(s)	738, 795
HapII	CCGG	2 cut(s)	312, 812
HhaI	GCGC	3 cut(s)	164, 767, 941
Hin1II	CATG	5 cut(s)	74, 305, 355, 373, 871
Hin6I	GCGC	3 cut(s)	162, 765, 939
HinP1I	GCGC	3 cut(s)	162, 765, 939
HindIII	AAGCTT	1 cut(s)	476
HinfI	GANTC	4 cut(s)	134, 279, 559, 659
HpaII	CCGG	2 cut(s)	312, 812
HphI	GGTGA	2 cut(s)	516, 590
Hpy166II	GTNNAC	1 cut(s)	865
Hpy188I	TCNGA	6 cut(s)	58, 114, 253, 284, 428, 466
Hpy188III	TCNNGA	5 cut(s)	103, 352, 563, 656, 889
Hpy8I	GTNNAC	1 cut(s)	865
HpyAV	CCTTC	4 cut(s)	143, 152, 235, 302
HpyCH4III	ACNGT	1 cut(s)	709
HpyF10VI	GCNNNNNNNGC	7 cut(s)	201, 210, 767, 815, 831, 938, 989
HpyF3I	CTNAG	1 cut(s)	960
Hsp92II	CATG	5 cut(s)	74, 305, 355, 373, 871
HspAI	GCGC	3 cut(s)	162, 765, 939
Ksp22I	TGATCA	1 cut(s)	358
Kzo9I	GATC	3 cut(s)	358, 616, 679
LmnI	GCTCC	2 cut(s)	467, 891
Lsp1109I	GCAGC	4 cut(s)	719, 757, 779, 818
LweI	GCATC	3 cut(s)	483, 639, 805
MaeI	CTAG	1 cut(s)	878
MaeIII	GTNAC	2 cut(s)	403, 578
MalI	GATC	3 cut(s)	360, 618, 681
MboI	GATC	3 cut(s)	358, 616, 679
MboII	GAAGA	3 cut(s)	103, 129, 778
MfeI	CAATTG	2 cut(s)	182, 417
MhlI	GDGCHC	2 cut(s)	827, 867
MluCI	AATT	5 cut(s)	5, 61, 182, 417, 539
MlyI	GAGTC	2 cut(s)	288, 653
MmeI	TCCRAC	2 cut(s)	36, 852
MnlI	CCTC	5 cut(s)	110, 238, 586, 832, 915
MseI	TTAA	4 cut(s)	236, 438, 588, 665
MslI	CAYNNNNRTG	1 cut(s)	300
MspA1I	CMGCKG	1 cut(s)	770
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	2 cut(s)	312, 812
MspR9I	CCNGG	3 cut(s)	218, 313, 812
MunI	CAATTG	2 cut(s)	182, 417
MvaI	CCWGG	1 cut(s)	218
MvnI	CGCG	1 cut(s)	939
MwoI	GCNNNNNNNGC	7 cut(s)	201, 210, 767, 815, 831, 938, 989
NciI	CCSGG	2 cut(s)	313, 812
NcoI	CCATGG	1 cut(s)	369
NdeII	GATC	3 cut(s)	358, 616, 679
NlaIII	CATG	5 cut(s)	74, 305, 355, 373, 871
NmuCI	GTSAC	1 cut(s)	578
NsbI	TGCGCA	1 cut(s)	766
NspI	RCATGY	2 cut(s)	74, 871
PagI	TCATGA	1 cut(s)	351
PciI	ACATGT	1 cut(s)	867
PfeI	GAWTC	2 cut(s)	134, 559
PkrI	GCNGC	6 cut(s)	87, 734, 737, 769, 772, 808
PleI	GAGTC	2 cut(s)	287, 653
PpsI	GAGTC	2 cut(s)	287, 653
PscI	ACATGT	1 cut(s)	867
Psp6I	CCWGG	1 cut(s)	216
PspGI	CCWGG	1 cut(s)	216
PspPI	GGNCC	1 cut(s)	794
PstI	CTGCAG	1 cut(s)	808
PvuII	CAGCTG	1 cut(s)	770
RsaI	GTAC	1 cut(s)	80
RsaNI	GTAC	1 cut(s)	79
RseI	CAYNNNNRTG	1 cut(s)	300
SaqAI	TTAA	4 cut(s)	236, 438, 588, 665
SatI	GCNGC	6 cut(s)	86, 733, 736, 768, 771, 807
Sau3AI	GATC	3 cut(s)	358, 616, 679
Sau96I	GGNCC	1 cut(s)	794
SchI	GAGTC	2 cut(s)	288, 653
ScrFI	CCNGG	3 cut(s)	218, 313, 812
SduI	GDGCHC	2 cut(s)	827, 867
SfaNI	GCATC	3 cut(s)	483, 639, 805
SfcI	CTRYAG	1 cut(s)	804
SmiMI	CAYNNNNRTG	1 cut(s)	300
SmlI	CTYRAG	2 cut(s)	101, 437
SmoI	CTYRAG	2 cut(s)	101, 437
Sse9I	AATT	5 cut(s)	5, 61, 182, 417, 539
SsiI	CCGC	4 cut(s)	85, 385, 496, 735
SspMI	CTAG	1 cut(s)	878
StyD4I	CCNGG	3 cut(s)	216, 311, 810
StyI	CCWWGG	3 cut(s)	369, 527, 790
TaaI	ACNGT	1 cut(s)	709
TaqI	TCGA	1 cut(s)	75
TasI	AATT	5 cut(s)	5, 61, 182, 417, 539
TatI	WGTACW	1 cut(s)	78
TauI	GCSGC	2 cut(s)	88, 738
TfiI	GAWTC	2 cut(s)	134, 559
Tru1I	TTAA	4 cut(s)	236, 438, 588, 665
Tru9I	TTAA	4 cut(s)	236, 438, 588, 665
TscAI	CASTG	2 cut(s)	350, 867
TseFI	GTSAC	1 cut(s)	578
TseI	GCWGC	4 cut(s)	732, 767, 770, 806
Tsp45I	GTSAC	1 cut(s)	578
TspDTI	ATGAA	6 cut(s)	17, 254, 284, 340, 368, 425
TspGWI	ACGGA	3 cut(s)	161, 245, 329
TspRI	CASTG	2 cut(s)	350, 867
Vha464I	CTTAAG	1 cut(s)	437
VneI	GTGCAC	1 cut(s)	863
XapI	RAATTY	1 cut(s)	5
XceI	RCATGY	2 cut(s)	74, 871
XspI	CTAG	1 cut(s)	878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.