Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006835

Physical Location & Seq

Genomic Coordinates Reverse (-)

5783020 .. 5807714

Gene Structure 24695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1051100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5787 bp

ATGGCACCGCCTTTCAAGAAACCTGAGGCCAAGAAGAAGGCCGGAGATGCTCCAAAGTTCAACAAGTCCTCCAAAAAACCGTTCAAACCAAACAAGGACCAAAACGACGACGTCAGGTCTGAAGCCGTCGCTTTGCAGCTCGAAGACGACGTCCCCGACTTCCCCAGAGGGGGAGGAAGTGCTTTGAATCGACAAGAGCGGGATGAAATTCGGGCCGAAGTCGATGCTGAATTTGAGGCAGAGGAGCGGGAGCTGAAGAAGAGGAAGAAGAAAGGGATGCAGAAGAATAGTGTCGCAACGAAGGAGGACGATTTGGGGTCGCTTTTCGGTGACGGTATTTCTGGAAAGCTACCTAAATATGCAAACAAGATCACTATGAAGAATATTTCTGCTGGAATGAAGGTCTGGGGAGTTGTCTCTGAAGTAAATGAAAAGGACCTCGTAATTAGTCTTCCAGGGGGATTGCGTGGATTAGTTCGAGCTTCTGAAGCTTTTGATCCTATTTTGGATGATGAAAATAAGGCTGTAGCAGATAGTCTCCTTCCTAGCGTATTCCATACTGGGCAGCTGGTATCTTGCATTGTATTGAAGTTAGATGAGGATAAGAAAGAGAAAGGGAAAAGGAAGATCTGGCTTTCTTTGCGCCTATCTTCGTTGTACAAGGGCTTCACTTTGGAGTCTGTTCAAGAAGGCATGGTCCTTACTGCATATGTTAAAAGCATTGAAGACCATGGTTATATTCTCCATTTTGGTTTATCTTCATTTACGGGATTTTTGCCAAAAAAGAGTGAATCTGACACCAAGGAGATTCAACTGAAAGCTGGGCAACTACTTCAAGGTGCTGTTAAAAGTGTTGATAAAATCCGCAAGGTTGTGTATTTGAGTTCTGACCCAGAAACTGTGTCAAAATGTGTGACCAAGGATCTCAAGGGAATTTCAATTGATCTACTCGTTCCAGGCATGATGGTTAATGCTCGTGTGCTATCGACCCTTGAAAATGGGGTTATGTTATCATTTCTAACTTACTTTACTGGAACTGTTGATTTATTCCATCTGCAAAATAGTTGCCCTACCATAAACTGGAAGGAGGAGTACAATCAACACAAGAAGGTTAATGCTCGAATATTGTTTATTGATCCTTCAACTAGAGCTGTTGGTTTGACACTGAATCCACATCTTGTCCGTAACAAGGCTCCTCCTTCACTTGTTAAAATCGGAGACATATGTGATGACTCAAAAGTGGTTAGGGTCGACAAAGGATTGGGCCTTCTGCTTGAAATACCATCTACCCCGGTTTCAACTCCGGCATACGTTAGTATATCTGATGTGGCTGAAGAGGATGCTCGAAAGCTTGAAAAAAAGTTCAAGCTAGGTAGTCGTGTTCGTGTCAGAATTCTTGGTTTTAGACACCTGGAAGGGCTTGCGACAGGGACTTTGAAGGCCAGCGCTATGGAAGGGACAGTCTTTACTCATTCTGATGTCAAGCCTGGGATGGTGGTGAGAGGTAAAATCATAGCTGTGGACAGCTTTGGTGCTATCGTGCAATTTTCTGGTGGTGTGAAGGCACTTTGCCCTCTTACTCATATGTCTGAATTTGAAATTGCAAAGCCTGGAAAAAAATTTAAGATTGGAGCTGAACTTCTGTTCCGTGTGCTTGGTTGCAAGTCCAAGAGAATAACTGTTACACACAAGAAAACACTTGTGAAATCGAAACATGGGATTGTTAGTTCATATGCTGAGGCTGCTGACGGATTAATCACCCATGGGTGGATAAGAAAGATTGAAGAACATGGATGCTTTGTCCATTTTTACAATGGAGTCCAAGGATTTGCTCCAAGATCTGAACTCGGATTGGAACCAGGATCTGACCCAAGTTCAATGTATCATGTTGGGCAAGTTGTCAAATGTAGAGTTATCAATTCCAATCCTACATCAAGGCGGATCAAACTTAGCTTCGTAATAAGGCCTGCAAGGGTTTCTGAGGATGATGTAGCCAAGTTGGGTTGCCTTGTTTCTGGAGTTGTTGATCGAGTAACCCCCAGTGCTGTTTATGTTAATGGAAAAGGTTACTCGATGGGAACAATTTTTACTGATCACTTGTCAGATCACCACGGGCTTGCTGCTTTGATGAGGTCAGTTTTGAAGCCTGGATATGAATTTGATCAATTGCTGGTGCTAGACATTGAAGGGAACAATTTGATCCTTTCTGCAAAATATTCTTTGATTAATTCACTTCAGCAGCTTCCCTCAGAACTCAGTCAAGTTCATCCTAACTCGCTAGTACATGGCTACATTTGTAACTTAATTGAAACTGGGTGTTTTGTTCGCTTTCTTGGGCGTTTGACTGGTTTCTCCCCCAGGCATAAGGCAATGGATGACCCTAAAGTTGATCTTTCAGAAACCTATTTCATTGGACAATCTGTTCGCAGTAATATACTAAATGTCAGTAGCGAATCAGGTCGAATAACACTGTCGTTGAAGCAGTCATCTTGCTCTTCAACAGATGTGTCCTTTATTCAGGAGTACTTTATGTTGGAGGAGAAGATTGCTACGCTGCAACTGTTAAACTCAAAAGAATCCAAGTCAAATTGGTCAGAAGGATTTACAATTGGCAGTGTTGTTGAGGGTAAAGTACAGGAGGTAAAGGATGTTGGAGTTGTTGTCAACTTTGAGAAATATAATGACGTCTTTGGTTTCATCACACACTACCAATTAGGTGGGACCGTTGTAGAAACAGGCTCCAGCGTTCATGCAGTTGTACTTGACGTTGCCAAGGCGGAGCACCTTGTTGATTTATCGATGAAACAAGAGTTCACTAATAAATTCAAAGAAAGTTCTGACAAGAAAAGTCATAAGAAGAAACGAAAAAGTGAAGCATTGGATGACCTGGAGGTACATCAGACAGTAAATGCAATTGTTGAGATTGTGAAAGAGAATTACCTGGTTCTTTCAATCCCCAAGTATAATTATGCTGTAGGATATGCATCAATCTCCGACTACAATACTCAAAAAGTTCCTCAGAAACAGTTTTTGAATGGACAAAGTGTTGATGCCACTGTTATGGCTCTTCCAAGCCCTACAACAACAGGTAGGTTGCTTTTACTACTTAAATCTATAAGTGAGTCCGAGACATCCAGTTCAAAACGGGCAAAGAAGAAGTCCAGCTATAAAGTGGGATCTATGGTTGAGGCAGAGATTACAGAAATAAAGCCTCTTGAACTAAGATTGAAATTTGGAATTGGCTTGCATGGGCGGGTTCACATTACAGAGCTAAATGATGAGTTTCTGGAAGACCCATTTAATAACTTCAGAATTGGGCAAACAGTGGCTGCAAAGATCGTTGCCAAGACTAATTATTCAGATAACAACAAAAAGAGCTACCAGTGGGATCTTTCCTTGAAATCTACAATGCTAACGGGTTCCTGTGAAATAGGGGAAAAGATTATAACTGAAGATCTTGATTTCTCAACCGGGCAATGTGTCTCTGGTTATGTTTATAAAGTGGATGGTGAATGGGTCTGGTTGACAATATCTCGAAGTGTTAGGGCTCAGCTTTTTATTCTCGACAGTGCTTGTGAGCCTAGTGAACTTAAAGAATTTCAGAAACGCTTTCACCTGGGAAATGCTGTATCTGGTTATGTCTTAAGTGTTAACAGGGAGAAAAAGCTATTGCGCCTGGTTCTGCATCCTTTTTCTCCCATCTCTGATAAAACTGTTGATCATGGGGTTCCAAAGATGGATGATTCACACATTAACATTATGAATGAGAATGTTACTGCTCATATTCGCGAGGGACGTGTTATAGGTGGGAGAATAATTAAAAAACTTCCAGGTGTTAGTGGAATGACTGTGCAAATTGGACCCCATCTTTATGGCAGGGTCCATTATACTGAACTTTACGACTCATGGGAGTCTGACCCATTGTCTGGATATCATGAAGGGCAATTTGTCAAATGCAAGGTCCTTGAACTTATCCGCTCTGTGAAGGGAACTTTCCACATTGATTTGTCTTTGCGCGCTTCTTTAGTTGGCGGGCTCCGTCCAGATTTTGTGGAACCCCAGGATGACACGCATTCTCAAACCAAACGTGTGGAAACGATCGAAGATCTTAATCCTAACATGGTGGTTCAGGGTTATGTTAAAAATGTAATACCAAAGGGGTGTTTTATTTTACTTTCGCGGAAGCTTGATGCGAAAATTCTCGTCTCCAATTTATCTGATGGATATGTTCAAGATCTCGAAAAGGAATTCCCAGTTGGAAAACTTGTAACTGGCAGGGTTTCATCTGTGGAGCCATTATCAAAGCGAGTTGAAGTCACCTTGAAGACATTGAACACAAGCAGTGTGCCACAATTTGGAAGTGATAATTTGGATAGCTTGCGTGTTGGAGATATAATCTCTGGCAGGGTAAAAAGGATGGAGTCATATGGTTTATTCATCACGATTGACAACACAAACTTGGTTGGACTATGCCATGTGTCGGAGCTTTCAGAAGATAAACTTGACAATATTGAAACTAAATACAAAACAGGAGAGAGGGTGACAGCAAAAGTATTGAAGGTGGACAAGGAAAGGCACCGAGTTTCTCTCGGGATGAAGGATGTTTATATTATGGACAATGATGACCTTGAGACGCCTTCAGAACAAGATCCTGATGAAACCATTGTGGAGAATGTTCTTACGGATGTTACTATGTCAGACATATGTCCCGAAAGCAATTCATTTTTTACCCAGGACATGGACGTCGATAGTAAAAATGCAGAATCCCAGTTTCTTGCACAAGCAGAATCTAGAGCTTTTGTTCCTCCGCTTGACGTCACTCTTGATGACATAGACCAATTCGACGGTGAAGATAAAGAGCATCTTGATGTAGACACTGTCAACGAAAAGAAAATGCAGCCGACAAAGAAAAAAGCTAAGGAAGAGAGGGAGCGCGAAATAAGGTCTGCCGAGGAAAGACTATTGGCAAATGATATACCCAGAACCGATGAGGAATATGAGAAACTAGTTAGAACTTCTCCAAATAGCAGTTTTGTCTGGATAAAATATATGGAATTTGTGCTCTCTGCATCTAATGTTGAGAAGGCACGCTCAATTGCTGAAAGGGCTCTGGCAACAATAAAGTTGGACAAAGAGACTGAGAAGGAGAAGCTCAATGTTTGGGTGGCTTATTTCAATTTGGAAAACAAATACGCAAGTCCTCCAGAGGAAGCTGTAAAGAAAGTCTTTCAAAGAGCAGTACAGTGTAATGATTCCAAAACGGTTCATTTAGCACTTTTAGGACTGTATGAAAGGACTGAGCAACACAGGTTGGCTGATGAGCTACTTGATAAAATGACAAAGAAGTTCAAGACTTCCTGCAAGGTTTGGCTAAGGCGGGTAAAGCTGCTCTTAACGCGACAGCAGGATGGGATTCAGGATGTTCTCAGTCGTGCTGAGAAAGTCCTTCCTAAGCATAAGCTTATCAAATTCAATTCCCAGACAGCTATCCTTGAGTTTAAATGTGGAGAACCCGAGAGAGGACGATCCATGTTTGAGAATATTTTGCGGAATAACCCTAAGAGAACAGACTTGTGGAGCGTCTACCTTGATCAAGAAATTCGACTTGGAAATAGCGATCTGATTCATGCACTATTTGAGAGGGCAACTAGTTTGAGTCTCCCAGCAAAAAAGATGAAGTTCTTATTTAAAAAGTATCTGGCGTACGAGAAGTCACGCGGCGATGAAGAAAAGATCGAGTATGTGAAACGGAAGGCAATGGAGTATGTGGAGAATACAGTAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0000462 GO:0000466 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009553 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

S1 Suf

Protein Analysis

1929

Amino Acids

215.75

Weight (kDa)

8.41

Isoelectric Point (pI)

36.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1_RRP5	PF23459	130 - 203	1.4e-06	RRP5 S1 domain
S1_RRP5	PF23459	318 - 389	2.8e-06	RRP5 S1 domain
OB_RRP5_4th	PF24685	398 - 489	1e-40	RRP5 OB-fold domain
S1	PF00575	494 - 558	1.7e-08	S1 RNA binding domain
S1_RRP5	PF23459	583 - 652	2.7e-07	RRP5 S1 domain
S1	PF00575	587 - 651	7e-07	S1 RNA binding domain
OB_RRP5	PF24682	951 - 1038	2.4e-40	RRP5 OB-fold domain
S1_RRP5	PF23459	1371 - 1440	5.6e-07	RRP5 S1 domain
S1	PF00575	1461 - 1533	6e-16	S1 RNA binding domain
HAT_Syf1_CNRKL1_N	PF23233	1642 - 1750	7.1e-06	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	1656 - 1749	2.3e-06	PRP39 N-terminal HAT repeat
HAT_PRP39_C	PF23241	1679 - 1796	5.2e-08	PRP39 C-terminal HAT repeat
Suf	PF05843	1827 - 1925	5.1e-06	Suppressor of forked protein (Suf)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11964 AT3G11964
fragaria_vesca	FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470
malus_domestica	MD12G1051100.v1.1
prunus_persica	Prupe.7G085700_v2.0.a1 Prupe.7G085700_v2.0.a1
pyrus_communis	pycom12g04550
rosa_chinensis	RchiOBHm_Chr3g0478661
rosa_laevigata	RLG00000023635
rosa_multiflora	Rmu_sc0002454.1_g000002 Rmu_sc0023704.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403220
rosa_rugosa	Rorug03G0166800 Rorug03G0166800 Rorug03G0166900
rosa_samantha	Rh3AG219000 Rh3BG250700 Rh3CG247500 Rh3DG243800
rosa_wichuraiana	Rw3G019690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3446, 3498
AasI	GACNNNNNNGTC	1 cut(s)	4742
AatII	GACGTC	5 cut(s)	114, 153, 2688, 4746, 4818
AccB1I	GGYRCC	2 cut(s)	4, 4575
AccB7I	CCANNNNNTGG	4 cut(s)	1080, 3926, 4355, 4738
AccBSI	CCGCTC	3 cut(s)	199, 247, 3978
AccI	GTMKAC	3 cut(s)	1251, 4872, 5583
AccII	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
AcuI	CTGAAG	9 cut(s)	141, 275, 441, 507, 1353, 2219, 3292, 3471, 4623
AcyI	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
AfaI	GTAC	9 cut(s)	659, 1094, 2283, 2525, 2634, 2760, 2895, 5241, 5705
AfeI	AGCGCT	1 cut(s)	1447
AflII	CTTAAG	1 cut(s)	3643
AflIII	ACRYGT	2 cut(s)	3796, 4087
AhdI	GACNNNNNGTC	2 cut(s)	115, 3922
AhlI	ACTAGT	2 cut(s)	5005, 5648
AjiI	CACGTC	1 cut(s)	3797
AjuI	GAANNNNNNNTTGG	6 cut(s)	65, 97, 4239, 4271, 5589, 5621
AloI	GAACNNNNNNTCC	6 cut(s)	1629, 1661, 1711, 1743, 2406, 2438
Alw21I	GWGCWC	2 cut(s)	2784, 5064
Alw26I	GTCTC	9 cut(s)	421, 542, 1212, 3122, 3487, 4210, 4625, 5129, 5663
AlwNI	CAGNNNCTG	3 cut(s)	899, 1865, 3329
Ama87I	CYCGRG	2 cut(s)	4589, 5513
Aor51HI	AGCGCT	1 cut(s)	1447
AoxI	GGCC	6 cut(s)	27, 39, 213, 1264, 1440, 1964
ApeKI	GCWGC	9 cut(s)	136, 565, 1742, 2120, 2239, 2554, 3329, 4897, 5386
AseI	ATTAAT	2 cut(s)	1754, 2226
AspLEI	GCGC	6 cut(s)	645, 1448, 3675, 4017, 4019, 4935
AspS9I	GGNCC	9 cut(s)	97, 213, 436, 697, 1264, 2721, 3860, 3880, 3961
AsuC2I	CCSGG	2 cut(s)	1292, 3472
AsuHPI	GGTGA	9 cut(s)	341, 1510, 1750, 2099, 3521, 3605, 4309, 4552, 4859
AvaI	CYCGRG	2 cut(s)	4589, 5513
AvaII	GGWCC	7 cut(s)	97, 436, 697, 2721, 3860, 3880, 3961
AxyI	CCTNAGG	1 cut(s)	24
BanI	GGYRCC	2 cut(s)	4, 4575
BanII	GRGCYC	3 cut(s)	3550, 4038, 5110
BarI	GAAGNNNNNNTAC	6 cut(s)	435, 467, 650, 682, 1076, 1108
BauI	CACGAG	1 cut(s)	975
BbsI	GAAGAC	5 cut(s)	150, 443, 732, 3297, 4331
Bbv12I	GWGCWC	2 cut(s)	2784, 5064
BbvCI	CCTCAGC	1 cut(s)	1737
BbvI	GCAGC	9 cut(s)	148, 577, 1729, 2107, 2251, 2541, 3316, 4909, 5373
BceAI	ACGGC	1 cut(s)	110
BcgI	CGANNNNNNTGC	2 cut(s)	206, 240
BclI	TGATCA	4 cut(s)	2092, 2161, 3718, 5590
BcnI	CCSGG	2 cut(s)	1292, 3472
BcoDI	GTCTC	9 cut(s)	421, 542, 1212, 3122, 3487, 4210, 4625, 5129, 5663
BcuI	ACTAGT	2 cut(s)	5005, 5648
BfaI	CTAG	9 cut(s)	546, 1146, 1370, 2177, 2279, 3582, 4791, 5006, 5649
BfmI	CTRYAG	2 cut(s)	525, 2973
BfoI	RGCGCY	1 cut(s)	1449
BfrI	CTTAAG	1 cut(s)	3643
BglII	AGATCT	5 cut(s)	627, 1838, 3454, 4105, 4234
BlpI	GCTNAGC	1 cut(s)	3549
BmcAI	AGTACT	1 cut(s)	2525
Bme18I	GGWCC	7 cut(s)	97, 436, 697, 2721, 3860, 3880, 3961
BmeRI	GACNNNNNGTC	2 cut(s)	115, 3922
BmeT110I	CYCGRG	2 cut(s)	4589, 5513
BmgBI	CACGTC	1 cut(s)	3797
BmgT120I	GGNCC	9 cut(s)	97, 213, 436, 697, 1264, 2721, 3860, 3880, 3961
BmrI	ACTGGG	5 cut(s)	570, 2034, 2322, 4247, 4762
BmuI	ACTGGG	5 cut(s)	570, 2034, 2322, 4247, 4762
BoxI	GACNNNNGTC	1 cut(s)	4704
BpiI	GAAGAC	5 cut(s)	150, 443, 732, 3297, 4331
BplI	GAGNNNNNCTC	2 cut(s)	4572, 4604
BpmI	CTGGAG	4 cut(s)	2037, 2725, 2909, 5187
Bpu10I	CCTNAGC	4 cut(s)	1737, 4917, 5372, 5451
Bpu1102I	GCTNAGC	1 cut(s)	3549
BpuEI	CTTGAG	3 cut(s)	911, 4649, 5513
BpuMI	CCSGG	2 cut(s)	1292, 3472
Bsa29I	ATCGAT	1 cut(s)	2798
BsaBI	GATNNNNATC	2 cut(s)	2983, 4110
BsaHI	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
BsaXI	ACNNNNNCTCC	6 cut(s)	53, 83, 2646, 2676, 4524, 4554
Bse21I	CCTNAGG	1 cut(s)	24
Bse3DI	GCAATG	3 cut(s)	2376, 3482, 5763
Bse8I	GATNNNNATC	2 cut(s)	2983, 4110
BseCI	ATCGAT	1 cut(s)	2798
BseJI	GATNNNNATC	2 cut(s)	2983, 4110
BseMI	GCAATG	3 cut(s)	2376, 3482, 5763
BsePI	GCGCGC	1 cut(s)	4015
BseRI	GAGGAG	4 cut(s)	257, 1103, 1185, 2552
BseXI	GCAGC	9 cut(s)	148, 577, 1729, 2107, 2251, 2541, 3316, 4909, 5373
BseYI	CCCAGC	2 cut(s)	821, 5662
Bsh1236I	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
Bsh1285I	CGRYCG	1 cut(s)	4101
BshFI	GGCC	6 cut(s)	29, 41, 215, 1266, 1442, 1966
BshNI	GGYRCC	2 cut(s)	4, 4575
BshVI	ATCGAT	1 cut(s)	2798
BsiEI	CGRYCG	1 cut(s)	4101
BsiHKAI	GWGCWC	2 cut(s)	2784, 5064
BsiHKCI	CYCGRG	2 cut(s)	4589, 5513
BsiSI	CCGG	4 cut(s)	42, 1292, 1304, 3471
BsiWI	CGTACG	1 cut(s)	5703
BslFI	GGGAC	6 cut(s)	137, 1444, 1471, 2734, 3807, 4692
BsmAI	GTCTC	9 cut(s)	421, 542, 1212, 3122, 3487, 4210, 4625, 5129, 5663
BsmBI	CGTCTC	2 cut(s)	4210, 4625
BsmFI	GGGAC	6 cut(s)	137, 1444, 1471, 2734, 3807, 4692
BsmI	GAATGC	1 cut(s)	4072
BsnI	GGCC	6 cut(s)	29, 41, 215, 1266, 1442, 1966
BsoBI	CYCGRG	2 cut(s)	4589, 5513
Bsp1286I	GDGCHC	5 cut(s)	2784, 3550, 4038, 5064, 5110
Bsp1407I	TGTACA	1 cut(s)	657
Bsp1720I	GCTNAGC	1 cut(s)	3549
Bsp19I	CCATGG	2 cut(s)	730, 1762
Bsp68I	TCGCGA	1 cut(s)	3789
BspANI	GGCC	6 cut(s)	29, 41, 215, 1266, 1442, 1966
BspDI	ATCGAT	1 cut(s)	2798
BspFNI	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
BspHI	TCATGA	1 cut(s)	3934
BspQI	GCTCTTC	2 cut(s)	2500, 3072
BspT107I	GGYRCC	2 cut(s)	4, 4575
BspTI	CTTAAG	1 cut(s)	3643
BsrBI	CCGCTC	3 cut(s)	199, 247, 3978
BsrDI	GCAATG	3 cut(s)	2376, 3482, 5763
BsrGI	TGTACA	1 cut(s)	657
BssHII	GCGCGC	1 cut(s)	4015
BssNI	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
BssSI	CACGAG	1 cut(s)	975
BssT1I	CCWWGG	6 cut(s)	730, 801, 918, 1762, 1822, 2772
Bst2BI	CACGAG	1 cut(s)	975
Bst6I	CTCTTC	5 cut(s)	254, 1329, 2500, 3072, 4917
BstACI	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
BstAFI	CTTAAG	1 cut(s)	3643
BstAUI	TGTACA	1 cut(s)	657
BstC8I	GCNNGC	9 cut(s)	1422, 1444, 1968, 2118, 3245, 4017, 4034, 4379, 5089
BstDSI	CCRYGG	3 cut(s)	730, 1762, 2110
BstFNI	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
BstH2I	RGCGCY	1 cut(s)	1449
BstHHI	GCGC	6 cut(s)	645, 1448, 3675, 4017, 4019, 4935
BstMAI	GTCTC	9 cut(s)	421, 542, 1212, 3122, 3487, 4210, 4625, 5129, 5663
BstMCI	CGRYCG	1 cut(s)	4101
BstMWI	GCNNNNNNNGC	7 cut(s)	47, 488, 640, 1742, 5105, 5383, 5395
BstPAI	GACNNNNGTC	1 cut(s)	4704
BstSFI	CTRYAG	2 cut(s)	525, 2973
BstUI	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
BstV1I	GCAGC	9 cut(s)	148, 577, 1729, 2107, 2251, 2541, 3316, 4909, 5373
BstV2I	GAAGAC	5 cut(s)	150, 443, 732, 3297, 4331
BstXI	CCANNNNNNTGG	5 cut(s)	1450, 2717, 3061, 3872, 4090
Bsu15I	ATCGAT	1 cut(s)	2798
Bsu36I	CCTNAGG	1 cut(s)	24
BsuRI	GGCC	6 cut(s)	29, 41, 215, 1266, 1442, 1966
BsuTUI	ATCGAT	1 cut(s)	2798
BtgI	CCRYGG	3 cut(s)	730, 1762, 2110
BtgZI	GCGATG	1 cut(s)	5736
BtrI	CACGTC	1 cut(s)	3797
BtsI	GCAGTG	2 cut(s)	2620, 4348
BtuMI	TCGCGA	1 cut(s)	3789
Cac8I	GCNNGC	9 cut(s)	1422, 1444, 1968, 2118, 3245, 4017, 4034, 4379, 5089
CaiI	CAGNNNCTG	3 cut(s)	899, 1865, 3329
CciI	TCATGA	1 cut(s)	3934
CfoI	GCGC	6 cut(s)	645, 1448, 3675, 4017, 4019, 4935
Cfr13I	GGNCC	9 cut(s)	97, 213, 436, 697, 1264, 2721, 3860, 3880, 3961
ClaI	ATCGAT	1 cut(s)	2798
CseI	GACGC	2 cut(s)	4642, 5569
CsiI	ACCWGGT	1 cut(s)	2940
Csp6I	GTAC	9 cut(s)	658, 1093, 2282, 2524, 2633, 2759, 2894, 5240, 5704
CviQI	GTAC	9 cut(s)	658, 1093, 2282, 2524, 2633, 2759, 2894, 5240, 5704
DraI	TTTAAA	2 cut(s)	5500, 5689
DrdI	GACNNNNNNGTC	1 cut(s)	4742
DriI	GACNNNNNGTC	2 cut(s)	115, 3922
DseDI	GACNNNNNNGTC	1 cut(s)	4742
Eam1104I	CTCTTC	5 cut(s)	254, 1329, 2500, 3072, 4917
Eam1105I	GACNNNNNGTC	2 cut(s)	115, 3922
EarI	CTCTTC	5 cut(s)	254, 1329, 2500, 3072, 4917
EciI	GGCGGA	2 cut(s)	1954, 2792
Eco130I	CCWWGG	6 cut(s)	730, 801, 918, 1762, 1822, 2772
Eco147I	AGGCCT	1 cut(s)	1966
Eco24I	GRGCYC	3 cut(s)	3550, 4038, 5110
Eco32I	GATATC	1 cut(s)	3932
Eco47I	GGWCC	7 cut(s)	97, 436, 697, 2721, 3860, 3880, 3961
Eco47III	AGCGCT	1 cut(s)	1447
Eco57I	CTGAAG	9 cut(s)	141, 275, 441, 507, 1353, 2219, 3292, 3471, 4623
Eco81I	CCTNAGG	1 cut(s)	24
Eco88I	CYCGRG	2 cut(s)	4589, 5513
EcoO109I	RGGNCCY	2 cut(s)	436, 3961
EcoRI	GAATTC	2 cut(s)	1392, 4247
EcoRV	GATATC	1 cut(s)	3932
EcoT14I	CCWWGG	6 cut(s)	730, 801, 918, 1762, 1822, 2772
EcoT22I	ATGCAT	1 cut(s)	2986
EcoT38I	GRGCYC	3 cut(s)	3550, 4038, 5110
ErhI	CCWWGG	6 cut(s)	730, 801, 918, 1762, 1822, 2772
Esp3I	CGTCTC	2 cut(s)	4210, 4625
FaqI	GGGAC	6 cut(s)	137, 1444, 1471, 2734, 3807, 4692
FauI	CCCGC	5 cut(s)	192, 240, 3246, 4025, 5370
FauNDI	CATATG	6 cut(s)	709, 1223, 1584, 1732, 4426, 4703
FbaI	TGATCA	4 cut(s)	2092, 2161, 3718, 5590
FblI	GTMKAC	3 cut(s)	1251, 4872, 5583
FriOI	GRGCYC	3 cut(s)	3550, 4038, 5110
FspBI	CTAG	9 cut(s)	546, 1146, 1370, 2177, 2279, 3582, 4791, 5006, 5649
GlaI	GCGC	6 cut(s)	644, 1447, 3674, 4016, 4018, 4934
GsaI	CCCAGC	2 cut(s)	825, 5666
GsuI	CTGGAG	4 cut(s)	2037, 2725, 2909, 5187
HaeII	RGCGCY	1 cut(s)	1449
HaeIII	GGCC	6 cut(s)	29, 41, 215, 1266, 1442, 1966
HapII	CCGG	4 cut(s)	42, 1292, 1304, 3471
HgaI	GACGC	2 cut(s)	4642, 5569
HhaI	GCGC	6 cut(s)	645, 1448, 3675, 4017, 4019, 4935
Hin1I	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
Hin6I	GCGC	6 cut(s)	643, 1446, 3673, 4015, 4017, 4933
HinP1I	GCGC	6 cut(s)	643, 1446, 3673, 4015, 4017, 4933
HincII	GTYRAC	5 cut(s)	1252, 2665, 3525, 3652, 4882
HindII	GTYRAC	5 cut(s)	1252, 2665, 3525, 3652, 4882
HindIII	AAGCTT	4 cut(s)	489, 1349, 4184, 5459
HpaI	GTTAAC	1 cut(s)	3652
HpaII	CCGG	4 cut(s)	42, 1292, 1304, 3471
HphI	GGTGA	9 cut(s)	341, 1510, 1750, 2099, 3521, 3605, 4309, 4552, 4859
Hpy99I	CGWCG	6 cut(s)	110, 113, 131, 152, 4748, 4847
HpyCH4IV	ACGT	9 cut(s)	111, 150, 1311, 2685, 2766, 3796, 4087, 4743, 4815
HpyF10VI	GCNNNNNNNGC	7 cut(s)	47, 488, 640, 1742, 5105, 5383, 5395
HpySE526I	ACGT	9 cut(s)	111, 150, 1311, 2685, 2766, 3796, 4087, 4743, 4815
Hsp92I	GRCGYC	6 cut(s)	111, 150, 2685, 4634, 4743, 4815
HspAI	GCGC	6 cut(s)	643, 1446, 3673, 4015, 4017, 4933
Ksp22I	TGATCA	4 cut(s)	2092, 2161, 3718, 5590
KspAI	GTTAAC	1 cut(s)	3652
LguI	GCTCTTC	2 cut(s)	2500, 3072
Lsp1109I	GCAGC	9 cut(s)	148, 577, 1729, 2107, 2251, 2541, 3316, 4909, 5373
MabI	ACCWGGT	1 cut(s)	2940
MaeI	CTAG	9 cut(s)	546, 1146, 1370, 2177, 2279, 3582, 4791, 5006, 5649
MaeII	ACGT	9 cut(s)	111, 150, 1311, 2685, 2766, 3796, 4087, 4743, 4815
MbiI	CCGCTC	3 cut(s)	199, 247, 3978
MfeI	CAATTG	5 cut(s)	939, 2165, 2607, 2913, 5094
MhlI	GDGCHC	5 cut(s)	2784, 3550, 4038, 5064, 5110
MlyI	GAGTC	8 cut(s)	686, 1226, 1827, 3131, 3896, 3920, 4430, 5665
MmeI	TCCRAC	8 cut(s)	2514, 2632, 3018, 4237, 4366, 4444, 4461, 5106
Mph1103I	ATGCAT	1 cut(s)	2986
MslI	CAYNNNNRTG	5 cut(s)	1476, 1517, 3059, 3870, 4866
MspA1I	CMGCKG	1 cut(s)	568
MspCI	CTTAAG	1 cut(s)	3643
MspI	CCGG	4 cut(s)	42, 1292, 1304, 3471
MunI	CAATTG	5 cut(s)	939, 2165, 2607, 2913, 5094
Mva1269I	GAATGC	1 cut(s)	4072
MvnI	CGCG	6 cut(s)	3789, 4017, 4180, 4935, 5398, 5718
MwoI	GCNNNNNNNGC	7 cut(s)	47, 488, 640, 1742, 5105, 5383, 5395
NciI	CCSGG	2 cut(s)	1292, 3472
NcoI	CCATGG	2 cut(s)	730, 1762
NdeI	CATATG	6 cut(s)	709, 1223, 1584, 1732, 4426, 4703
NmeAIII	GCCGAG	1 cut(s)	4975
NmuCI	GTSAC	6 cut(s)	329, 913, 4315, 4540, 4816, 5712
NruI	TCGCGA	1 cut(s)	3789
NsiI	ATGCAT	1 cut(s)	2986
PagI	TCATGA	1 cut(s)	3934
PauI	GCGCGC	1 cut(s)	4015
PceI	AGGCCT	1 cut(s)	1966
PciSI	GCTCTTC	2 cut(s)	2500, 3072
PcsI	WCGNNNNNNNCGW	3 cut(s)	147, 153, 3793
PctI	GAATGC	1 cut(s)	4072
Pfl23II	CGTACG	1 cut(s)	5703
PflFI	GACNNNGTC	3 cut(s)	110, 149, 4877
PflMI	CCANNNNNTGG	4 cut(s)	1080, 3926, 4355, 4738
Ple19I	CGATCG	1 cut(s)	4101
PleI	GAGTC	8 cut(s)	685, 1226, 1826, 3130, 3896, 3919, 4429, 5664
PpsI	GAGTC	8 cut(s)	685, 1226, 1826, 3130, 3896, 3919, 4429, 5664
PpuMI	RGGWCCY	2 cut(s)	436, 3961
PshAI	GACNNNNGTC	1 cut(s)	4704
PshBI	ATTAAT	2 cut(s)	1754, 2226
PsiI	TTATAA	2 cut(s)	3446, 3498
Psp5II	RGGWCCY	2 cut(s)	436, 3961
PspFI	CCCAGC	2 cut(s)	821, 5662
PspLI	CGTACG	1 cut(s)	5703
PspPI	GGNCC	9 cut(s)	97, 213, 436, 697, 1264, 2721, 3860, 3880, 3961
PspPPI	RGGWCCY	2 cut(s)	436, 3961
PsrI	GAACNNNNNNTAC	2 cut(s)	2071, 2103
PstNI	CAGNNNCTG	3 cut(s)	899, 1865, 3329
PsyI	GACNNNGTC	3 cut(s)	110, 149, 4877
PteI	GCGCGC	1 cut(s)	4015
PvuI	CGATCG	1 cut(s)	4101
PvuII	CAGCTG	1 cut(s)	568
RruI	TCGCGA	1 cut(s)	3789
RsaI	GTAC	9 cut(s)	659, 1094, 2283, 2525, 2634, 2760, 2895, 5241, 5705
RsaNI	GTAC	9 cut(s)	658, 1093, 2282, 2524, 2633, 2759, 2894, 5240, 5704
RseI	CAYNNNNRTG	5 cut(s)	1476, 1517, 3059, 3870, 4866
SalI	GTCGAC	1 cut(s)	1250
SapI	GCTCTTC	2 cut(s)	2500, 3072
Sau96I	GGNCC	9 cut(s)	97, 213, 436, 697, 1264, 2721, 3860, 3880, 3961
ScaI	AGTACT	1 cut(s)	2525
SchI	GAGTC	8 cut(s)	686, 1226, 1827, 3131, 3896, 3920, 4430, 5665
SduI	GDGCHC	5 cut(s)	2784, 3550, 4038, 5064, 5110
SexAI	ACCWGGT	1 cut(s)	2940
SfcI	CTRYAG	2 cut(s)	525, 2973
SinI	GGWCC	7 cut(s)	97, 436, 697, 2721, 3860, 3880, 3961
SmiMI	CAYNNNNRTG	5 cut(s)	1476, 1517, 3059, 3870, 4866
SmlI	CTYRAG	4 cut(s)	926, 3643, 4628, 5492
SmoI	CTYRAG	4 cut(s)	926, 3643, 4628, 5492
SpeI	ACTAGT	2 cut(s)	5005, 5648
SseBI	AGGCCT	1 cut(s)	1966
SspI	AATATT	5 cut(s)	385, 1125, 2216, 4510, 5542
SspMI	CTAG	9 cut(s)	546, 1146, 1370, 2177, 2279, 3582, 4791, 5006, 5649
StuI	AGGCCT	1 cut(s)	1966
StyI	CCWWGG	6 cut(s)	730, 801, 918, 1762, 1822, 2772
TaiI	ACGT	9 cut(s)	114, 153, 1314, 2688, 2769, 3799, 4090, 4746, 4818
TatI	WGTACW	7 cut(s)	657, 1092, 2281, 2523, 2632, 2758, 5239
TauI	GCSGC	1 cut(s)	5721
TseFI	GTSAC	6 cut(s)	329, 913, 4315, 4540, 4816, 5712
TseI	GCWGC	9 cut(s)	136, 565, 1742, 2120, 2239, 2554, 3329, 4897, 5386
Tsp45I	GTSAC	6 cut(s)	329, 913, 4315, 4540, 4816, 5712
TspGWI	ACGGA	6 cut(s)	1172, 1637, 1764, 4028, 4697, 5764
Tth111I	GACNNNGTC	3 cut(s)	110, 149, 4877
Van91I	CCANNNNNTGG	4 cut(s)	1080, 3926, 4355, 4738
Vha464I	CTTAAG	1 cut(s)	3643
VpaK11BI	GGWCC	7 cut(s)	97, 436, 697, 2721, 3860, 3880, 3961
VspI	ATTAAT	2 cut(s)	1754, 2226
XbaI	TCTAGA	1 cut(s)	4790
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1811, 3169
XmiI	GTMKAC	3 cut(s)	1251, 4872, 5583
XspI	CTAG	9 cut(s)	546, 1146, 1370, 2177, 2279, 3582, 4791, 5006, 5649
ZraI	GACGTC	5 cut(s)	112, 151, 2686, 4744, 4816
ZrmI	AGTACT	1 cut(s)	2525
Zsp2I	ATGCAT	1 cut(s)	2986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.