Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0006835

Physical Location & Seq

Genomic Coordinates Reverse (-)

22763072 .. 22779650

Gene Structure 16579 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G019690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5793 bp

ATGGCACCGTCGTCCAAAAAACCTCAGGGAGGGAATAACAAGAATCCCAGAGACGCTCCAAAGTTCAACAAATCCTCCAACAAACCTTTCAAACCAAACAAGGATCACAACAACGCCGCCAGGTCCGAAGCCATGGCTTTGCAGCTCGAAGACGACGTCCCCGACTTCCCCAGAGGTGGAGGGAGCTCATTGAATCGAAAAGAACGCGACGAAATTCGGGCGGAAGTTGATGCCGAATTCGAGGCCGAGGAGAGGGAGATGAAGAAGAAAGGGAAGAAGAAAAGGGTGCCGAAGGACAGCCTTGTAAACGAGGACGAGATGGGCTCGCTTTTCGGGGATGGCATCACTGGAAAACTACCTAGATATGCAAACAAGATCACTTTGAAGAATATATCCCCTGGGATGAGGGTTTGGGGAGTTGTTGCCGAAGTGAATGAAAAGGACCTTGTAATCGGTCTTCCAGGGGGCTTGCGTGGACTAGTTCGAGCGTCTGATGCTTTTGATCCTATTCTGGATGATGAAACTGAGGCCTTAGCAGATAGTGTCCTCCCTAGCATATTCCGGATAGGGCAGCTGGTATCTTGCATTGTGTTGCAATTGGATGAGGATAAGAAAGAGAAAGGGAAACGGAAGATCTGGCTTTCTTTGCGCCTCTCTTTGTTGCATAAAGGCTTCACTTTGGATTCTGTTCAAGAAGGCATGGTCCTTACAGCCTATATTAAAAGCATCGAAGACCATGGTTACATTCTTCATTTTGGTTTATCTTCATTCACAGGGTTTTTGCCAAAAAATAGCCAAGTTGACAGCAAGGTTCAAGTGAGCAGTGGTCAACTGCTTCAAGCTGCTGTTAGAAGAGTTGATAAAACCCGCAAGGTTGTTCATATGAGTTCTGACCCAGAGATGGTGTCAAAGTGTGTGACCAAAGATCTCAAGGGAATTTCAATTGATCTCCTTGTTCCGGGGATGATGGTCAATGCTCGTGTGTTATCCACCCTTGAAAATGGGGTTATGTTATCATTTCTTACTTATTTTACTGGAACTGTTGATATATTTCATCTGCAAAATATTTACCCTACTACAAACTGGAAGGAAGATTACAATCAAAACAAGAAGCTCAATGCTCGAGTATTATTTGTCGATCCTTCAACTAGAGCTGTTGGTTTGACACTAAATCCACATCTTGTTCGCAACAAGGCTCCTCCTTCACATGTCAAAATTGGGGATATATGTGACGATGCAAAGGTAGTGAGGGTTGATAGAGGATTGGGCCTTCTGCTTGAAATACCATCTACTCAAGTTTCAACTCCGGCATATGTTAGTATATCTGATGTGGCTGAAGAAGTTCGGAAGCTTGAAAAGAAGTTCAAGGAAGGTACTCGTGTTCGTGTCAGAATTCTTGGGTTTAGGCACTTGGAAGGGCTTGCCACAGGGATTTTGAAGGCAAGTGCATTTGAAGGGTCAGTTTTCACTCACTCTGATGTCAAACCTGGGATGGTTGTGAGAGGTAAAATAATAGCTGTTGACAGCTTTGGGGCTATCGTGCAATTCCCTGGTGGTGTGAAGGCTCTTTGTCCTCTTACTCATATGTCTGAATTTGAAATTGCAAAGCCCAGAAAAAAATTTAAGGTTGGGGCTGAACTTGTGTTCCGTGTGCTTGGTTGCAAGTCCAAGAGAATAACTGTTACACACAAGAAAACACTCGTAAAGTCAAAGCTAGGGATTCTTAGTTCATATGCTGATGCTGCTGATGGATTAATAACACATGGGTGGATAAGAAAGATTGAAGAACGTGGCTGCTTTGTCCATTTTTACAATGGAGTCCAAGGATTTGCCCCAAGATCTGAACTCGGATTGGAGCCAGGATCTGACCCAAGTTCAATGTATCATGTTGGACAAGTTATCAAATGTAGAGTTCTCGGCTCTAATGCTACATCGAGACGAATCAAACTTAGCTTGATAATACGGCCTCCAAGGGTTTCTGAGGATGATATGGTGAAGCTGGGCAGCCTGGTTTCAGGAGTTGTTGACCGGGTAACCCCCAATGCTGTCATGGTTTATGTTAATGCAAAAGGTTACTCAATGGGCACAATTTTTACAGACCACTTGGCAGATCACCATGGGCTGGCTGCTTTGATGAAGTCAGTGTTGAAGCCTGGTTATGAATTTGATCAATTATTGGTTTTAGACACTGAAGGCAACAATTTGATCCTATCTGCAAAACATTCTTTGATTAATTCAGCTCCCATGCTTCCTTCAGAAGTCAGTCAAGTTCATCCAAATACGGTTGTACATGGCTACATTTGTAACTTAATTGACACTGGGTGCTTTGTTCGCTTTCTTGGGCGTGTGACTGGTTTCTCCCCAAGGCACAAGGCAATGGATGACCATAAAGGGGATCTTTCAGAAGCCTATTACATTGGACAGTCTGTTCGGAGTACTATACTCGATGTCAATAGTGAAACAGGTAGGATAACACTATCATTGAAGCAGTCATCTTGCTCTTCAACAGATGCCTCCTTTATCCGGGAATACTTTATTACAGAGGAGAAGATTGCAAAGCTGCAAGTTTTGGATTCAAAAGAATCCAAATCAGATTGGTCAGAAGGATTTTCCATTGGCAGTGTTGTTGAAGGGAAGGTCCAGGAGGCAAAGGACATTGGAGTTGTTGTCAGCTTTGAGAAATATAATGATGTTTTCGGTTTCATCACGCACTATCAATTAGCTGGTACCACTGTAGAAACAGGCTCCATTGTTCGAGCAGTTGTACTTGATGTTGCCAAGGCAGAGCACCTTGTTGACTTATCGCTCAAACCAGAATTTATTACCAACTTAAAACAAGAAAGCTCTAAGAGCCAAACTCATATGAAGAAACGAAGAAGAGAAGTCTCGGATGATCCAGAGATGCATGAGACCGTAAATGCTGTGGTTGAGATTGTGAAGGAGAATTACCTGGTTCTTTCTATCCCAAAGTATAACTATGCTGTAGGATATGCATCAATTTCTGACTACAACACTCAAAAGTTTCCTCAGAAACAGTTTCTGAATGGACAAAGTGTCAATACTACTGTCATGGCCCTTCCAAGCCCTACAACCGTTGGGAGATTGCTTTTGGTACTTAATTCTTTGAGTGAGTCCGCTGATACTTCCAGTTCAAAGCGGGCAAAGAAGAAATCCAGCTACAAAGTGGGATCTGTGGTCCAGGCAGAGATAACTGAAATAAAGCCTCTTGAACTAAGATTGAAGTTTGGAATCGGCTTTCATGGACGGGTCCGTATAACAGAGGTAAATGATGATCTTTTGGAAGATCCATTTAATAACTTCAGAATTGGGCAAACGGTCACTGCAAGGATTGTTGCAAAGACTAATTATTCAGATAACAACAAAAAGAGTTTCCAGTGGGATCTGTCATTGAAACCTAGTTTGTTAACAGGTTCCTGTGAAATAGAGGAAAATATTTTGAAAGGAGATCTCGATTTCTCAATTGGAAAACATGTCACTGGCTATGTGTACAAGGTGGATGCTGAATGGATCTGGTTAACAATATCTCGAAATGTTAGGGCCCAGCTTTTCATTCTTGACAGCGCTTGTGAGCCTAGTGAACTTCAAGAGTTTCAGAAACGCTTTCATGTTGGAAATGCTGTATCTGGTCATGTTTTAAGTGTTAGCAAGGAGAAAAAGCTATTACGGCTGGTTTCATATCCTTTTACTCCCGTCGCTGATAAAGCTGTTGATAATGAGGTGTCAAAGACAGATGCAAATGTTAGCATGTTGAATGCAACTGCTCATATTCGCGAGGGATGTGTTGTAGGTGGGAGAATTATTAAAAAACTTCCAGGTGTTGGTGGATTGACTGTGCAAATTGGTCCCCATATGTATGGCAGGGTTCATTATAGTGAGCTTTCTGACTCATGGTTGTCCAACCCATTGTCTGGATATGAGGAAGGTCAATTTGTCAAATGCAAGGTCGTTGAATGTAGTCGCTCTGGACAGGGAACTTTTCACATTGACTTGTCATTACGGTCTTCTTTAGTTGGCACACTTTGTCAAGATTCCAATGAACCTGACGATGACAAGCATACTCATACCGAACGGACGGAAAAGATTGACGATCTGAAACCTAATATGGTCATACAGGGTTATGTGAAAAATGTTTCATCAAAAGGATGTTTTATTTTGCTTTCGCGGAAGCTTGATGCCAAAATTTCGTCTCCAATTTATCCGATGGATATGTTGATGATCCTGAAAAGGAATTCCCAGTTGGAAAACTTGTTACCGGCAGGTAAGGTGTCATCTGTGGAGCCTTTATCAAAGAGAGTTGAAGTTACCTTGAAGTCACTGAGTGCAAGCAGTTTGCCACAATCTGCAAGGAATAATTTGGATACCTTGCATGTCGGAGATATTATCTCTGGCAGGGTAAAACGAGTTGAGTCATATGGATTATTCATCACAATTGACAATACAAATGTGGTTGGATTATGCCACGTGTCAGAGCTTGCAGAAGATAAGAAGGACAATTTTGAAACTAAATACAAAACAGGAGAGAGGGTGTCAGCAAAAGTATTGAAGGTGGACAAGGAGAGGCACCGAGTTTCTCTCGGAATGAAGGATTTGTATATTATGGATAGCGACCAAACACCTCCAAAACAAGATCTTGATGAACCTATCAGACAGACTGATTTGGTGGATGATTCTAAGTCAGTTATGTGTCCTGTTGGCAGTTTCCTTGGGGACCTGAACATGGATATTGAACATGAAAATGCAGAGTTCCAGTTTCTTTCACAAGCAGAATCTAGAGCTTTAGTTCCTCCACTTCAAGTCACTCTTGATGATGTAGACCAAGGCGATGATATTGTTAGCCAGGATCAAGAGCTTCCTGATGCGGAGGATGCTGTAGATGACAAGAAAAAGAAGCTCACGAAGAAAAAAGCCAGGGATGAGAGGGAGCGTGAGATAAGGGCTGCTGAGGAAAGATTATTGGAGAAAGATATACCTAGGACTGATGAGGAATTTGAGAAACTAGTGAGAAGTTCTCCAAATAACAGTTATGTTTGGATAAAATATATGGAATTTGTGCTCTCTATGGCTGATGTTGAGAAGGCACGCTCTATTGCTAAAAGGGCTTTGGAGACGATAAACTTTCGAGAAGAGAACGAGAAGCTCAATGTTTGGGTGGCTTATTTTAATTTGGAAAGCAAATATGGAAGTCCTCCAGAGGAGGCTGTAATGAAAGTCTTCCAAAAAGCGTTGCAGTATAATGATCCCAAAAAGGTTCATCTAGCACTCTTGGGAGTCTTTGAGAGGTCTGAGCAACACAAGTTGGCTGATGAACTTGCTGATAACATGATTAAGAGGTTTAAGAAGTCTTGCAAGGTTTGGCTAAGGCGGGTACAAAGGCTCTTGGTGCAACACCAGGATGGGGTTCAGGATTTTGTCTCTCGTGCTGAAAAGATCCTTCCTAAGCATAAGTATATCAAATTCTTGTCACAGACAGCCATCCTGGAGTTCAAATGTGGGAATCCAGAGAGAGGGCGATCCACGTTTGAAAATATTTTGAGGCAAAACCCGAAGAGAACAGACTTGTGGAGTGTTTATCTTGATCAAGAAATTCGACTTGGAGATACTGATCTGATTCGTGCATTGTTTGAGAGGGCAACTAGTTTGAGCCTACCAGCTAAAAAGATGAAATTCTTGTTCAAAAAATATCTGGAATATGAGGAGCGTCACGGTAACGAAGAGAGGGCCAATTACGTGAAACAGAAGGCAATGAGTTATGTTGAGAATACAGTAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0000462 GO:0000466 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009553 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

S1 Suf

Protein Analysis

1930

Amino Acids

215.9

Weight (kDa)

8.72

Isoelectric Point (pI)

37.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1_RRP5	PF23459	319 - 390	1e-06	RRP5 S1 domain
OB_RRP5_4th	PF24685	399 - 489	4.2e-40	RRP5 OB-fold domain
S1	PF00575	494 - 558	3.8e-08	S1 RNA binding domain
S1	PF00575	588 - 652	4.3e-06	S1 RNA binding domain
S1	PF00575	759 - 829	1.9e-07	S1 RNA binding domain
OB_RRP5	PF24682	954 - 1041	1.4e-37	RRP5 OB-fold domain
S1	PF00575	1463 - 1535	2.5e-16	S1 RNA binding domain
HAT_Syf1_CNRKL1_C	PF23231	1678 - 1927	3.4e-11	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
Suf	PF05843	1828 - 1926	1.1e-06	Suppressor of forked protein (Suf)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11964 AT3G11964
fragaria_vesca	FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470
malus_domestica	MD12G1051100.v1.1
prunus_persica	Prupe.7G085700_v2.0.a1 Prupe.7G085700_v2.0.a1
pyrus_communis	pycom12g04550
rosa_chinensis	RchiOBHm_Chr3g0478661
rosa_laevigata	RLG00000023635
rosa_multiflora	Rmu_sc0002454.1_g000002 Rmu_sc0023704.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403220
rosa_rugosa	Rorug03G0166800 Rorug03G0166800 Rorug03G0166900
rosa_samantha	Rh3AG219000 Rh3BG250700 Rh3CG247500 Rh3DG243800
rosa_wichuraiana	Rw3G019690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	159
Acc36I	ACCTGC	1 cut(s)	4268
Acc65I	GGTACC	1 cut(s)	2726
AccB1I	GGYRCC	4 cut(s)	4, 286, 2726, 4581
AccB7I	CCANNNNNTGG	3 cut(s)	2122, 3839, 3929
AccI	GTMKAC	1 cut(s)	4833
AccII	CGCG	3 cut(s)	207, 3792, 4183
AccIII	TCCGGA	1 cut(s)	561
AciI	CCGC	8 cut(s)	117, 221, 868, 3135, 3157, 4183, 4880, 5383
AcuI	CTGAAG	4 cut(s)	1356, 2211, 2238, 3304
AcvI	CACGTG	1 cut(s)	4483
AcyI	GRCGYC	1 cut(s)	156
AdeI	CACNNNGTG	2 cut(s)	2322, 4340
AfaI	GTAC	8 cut(s)	1375, 2289, 2437, 2728, 2766, 3114, 3509, 5388
AfeI	AGCGCT	1 cut(s)	3583
AflIII	ACRYGT	3 cut(s)	1207, 3490, 4482
AhdI	GACNNNNNGTC	1 cut(s)	3053
AhlI	ACTAGT	3 cut(s)	478, 5017, 5654
AjuI	GAANNNNNNNTTGG	6 cut(s)	71, 103, 4241, 4273, 5595, 5627
AleI	CACNNNNGTG	1 cut(s)	1765
AloI	GAACNNNNNNTCC	6 cut(s)	1629, 1661, 1711, 1743, 2412, 2444
Alw21I	GWGCWC	3 cut(s)	188, 2790, 5076
Alw26I	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4212, 5120, 5437
AlwNI	CAGNNNCTG	2 cut(s)	1865, 3040
Ama87I	CYCGRG	1 cut(s)	1122
Aor13HI	TCCGGA	1 cut(s)	561
Aor51HI	AGCGCT	1 cut(s)	3583
AoxI	GGCC	7 cut(s)	243, 528, 1267, 1964, 3072, 3558, 5739
ApaI	GGGCCC	1 cut(s)	3562
ApeKI	GCWGC	9 cut(s)	142, 571, 842, 1742, 1794, 2004, 2126, 2560, 4958
ArsI	GACNNNNNNTTYG	6 cut(s)	141, 173, 2412, 2444, 3948, 3980
AseI	ATTAAT	2 cut(s)	1754, 2232
Asp700I	GAANNNNTTC	2 cut(s)	1341, 5231
Asp718I	GGTACC	1 cut(s)	2726
AspA2I	CCTAGG	1 cut(s)	4991
AspLEI	GCGC	2 cut(s)	651, 3584
AsuC2I	CCSGG	3 cut(s)	960, 2030, 2525
AsuHPI	GGTGA	2 cut(s)	2005, 2105
AvaI	CYCGRG	1 cut(s)	1122
AvaII	GGWCC	8 cut(s)	123, 442, 703, 2638, 3196, 3268, 3863, 4729
AvrII	CCTAGG	1 cut(s)	4991
AxyI	CCTNAGG	1 cut(s)	24
BaeGI	GKGCMC	2 cut(s)	2087, 3562
BaeI	ACNNNNGTAYC	4 cut(s)	1365, 1398, 2718, 2751
BanI	GGYRCC	4 cut(s)	4, 286, 2726, 4581
BanII	GRGCYC	3 cut(s)	188, 326, 3562
BarI	GAAGNNNNNNTAC	6 cut(s)	441, 473, 1079, 1111, 2930, 2962
BauI	CACGAG	3 cut(s)	978, 1377, 5436
BbrPI	CACGTG	1 cut(s)	4483
BbsI	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5224
Bbv12I	GWGCWC	3 cut(s)	188, 2790, 5076
BbvCI	CCTCAGC	1 cut(s)	4962
BbvI	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4945
BceAI	ACGGC	2 cut(s)	1979, 3701
BciVI	GTATCC	1 cut(s)	4372
BclI	TGATCA	2 cut(s)	2167, 5596
BcnI	CCSGG	3 cut(s)	960, 2030, 2525
BcoDI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4212, 5120, 5437
BcuI	ACTAGT	3 cut(s)	478, 5017, 5654
BfmI	CTRYAG	3 cut(s)	2733, 2982, 4890
BfoI	RGCGCY	1 cut(s)	3585
BfuAI	ACCTGC	1 cut(s)	4268
BfuI	GTATCC	1 cut(s)	4372
BglII	AGATCT	5 cut(s)	633, 925, 1838, 3466, 4648
BlnI	CCTAGG	1 cut(s)	4991
BmcAI	AGTACT	1 cut(s)	2437
Bme18I	GGWCC	8 cut(s)	123, 442, 703, 2638, 3196, 3268, 3863, 4729
BmeRI	GACNNNNNGTC	1 cut(s)	3053
BmeT110I	CYCGRG	1 cut(s)	1122
BmrI	ACTGGG	2 cut(s)	2328, 4249
BmuI	ACTGGG	2 cut(s)	2328, 4249
BpiI	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5224
BplI	GAGNNNNNCTC	8 cut(s)	308, 340, 2842, 2874, 4578, 4610, 5014, 5046
BpmI	CTGGAG	2 cut(s)	5193, 5519
Bpu10I	CCTNAGC	4 cut(s)	532, 4962, 5378, 5457
BpuEI	CTTGAG	2 cut(s)	914, 1278
BpuMI	CCSGG	3 cut(s)	960, 2030, 2525
BsaAI	YACGTR	2 cut(s)	4483, 5749
BsaBI	GATNNNNATC	3 cut(s)	1098, 2992, 5622
BsaHI	GRCGYC	1 cut(s)	156
BsaI	GGTCTC	1 cut(s)	2903
BsaWI	WCCGGW	1 cut(s)	561
BsaXI	ACNNNNNCTCC	6 cut(s)	59, 89, 2652, 2682, 4530, 4560
Bse118I	RCCGGY	1 cut(s)	4273
Bse21I	CCTNAGG	1 cut(s)	24
Bse3DI	GCAATG	2 cut(s)	2382, 5769
Bse8I	GATNNNNATC	3 cut(s)	1098, 2992, 5622
BseAI	TCCGGA	1 cut(s)	561
BseJI	GATNNNNATC	3 cut(s)	1098, 2992, 5622
BseMI	GCAATG	2 cut(s)	2382, 5769
BseMII	CTCAG	7 cut(s)	38, 516, 1971, 3041, 4328, 4953, 5295
BseRI	GAGGAG	5 cut(s)	263, 1188, 2558, 5228, 5729
BseSI	GKGCMC	2 cut(s)	2087, 3562
BseXI	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4945
BseYI	CCCAGC	2 cut(s)	1999, 3561
Bsh1236I	CGCG	3 cut(s)	207, 3792, 4183
BshFI	GGCC	7 cut(s)	245, 530, 1269, 1966, 3074, 3560, 5741
BshNI	GGYRCC	4 cut(s)	4, 286, 2726, 4581
BsiHKAI	GWGCWC	3 cut(s)	188, 2790, 5076
BsiHKCI	CYCGRG	1 cut(s)	1122
BsiSI	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4274
BslFI	GGGAC	3 cut(s)	143, 3849, 4742
BsmAI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4212, 5120, 5437
BsmBI	CGTCTC	4 cut(s)	45, 1930, 4212, 5120
BsmFI	GGGAC	3 cut(s)	143, 3849, 4742
BsmI	GAATGC	1 cut(s)	3778
BsnI	GGCC	7 cut(s)	245, 530, 1269, 1966, 3074, 3560, 5741
Bso31I	GGTCTC	1 cut(s)	2903
BsoBI	CYCGRG	1 cut(s)	1122
Bsp120I	GGGCCC	1 cut(s)	3558
Bsp1286I	GDGCHC	6 cut(s)	188, 326, 2087, 2790, 3562, 5076
Bsp13I	TCCGGA	1 cut(s)	561
Bsp1407I	TGTACA	2 cut(s)	2287, 3507
Bsp19I	CCATGG	3 cut(s)	132, 736, 2116
Bsp68I	TCGCGA	1 cut(s)	3792
BspACI	CCGC	8 cut(s)	117, 221, 868, 3135, 3157, 4183, 4880, 5383
BspANI	GGCC	7 cut(s)	245, 530, 1269, 1966, 3074, 3560, 5741
BspCNI	CTCAG	7 cut(s)	37, 517, 1972, 3040, 4329, 4954, 5296
BspEI	TCCGGA	1 cut(s)	561
BspFNI	CGCG	3 cut(s)	207, 3792, 4183
BspMI	ACCTGC	1 cut(s)	4268
BspQI	GCTCTTC	1 cut(s)	2506
BspT107I	GGYRCC	4 cut(s)	4, 286, 2726, 4581
BspTNI	GGTCTC	1 cut(s)	2903
BsrDI	GCAATG	2 cut(s)	2382, 5769
BsrFI	RCCGGY	1 cut(s)	4273
BsrGI	TGTACA	2 cut(s)	2287, 3507
BssAI	RCCGGY	1 cut(s)	4273
BssNI	GRCGYC	1 cut(s)	156
BssSI	CACGAG	3 cut(s)	978, 1377, 5436
Bst2BI	CACGAG	3 cut(s)	978, 1377, 5436
Bst6I	CTCTTC	6 cut(s)	847, 2506, 2872, 5139, 5561, 5727
BstACI	GRCGYC	1 cut(s)	156
BstAUI	TGTACA	2 cut(s)	2287, 3507
BstBAI	YACGTR	2 cut(s)	4483, 5749
BstC8I	GCNNGC	8 cut(s)	326, 470, 1422, 2124, 3159, 4345, 4494, 5101
BstDSI	CCRYGG	3 cut(s)	132, 736, 2116
BstEII	GGTNACC	1 cut(s)	2032
BstENI	CCTNNNNNAGG	1 cut(s)	27
BstFNI	CGCG	3 cut(s)	207, 3792, 4183
BstH2I	RGCGCY	1 cut(s)	3585
BstHHI	GCGC	2 cut(s)	651, 3584
BstMAI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4212, 5120, 5437
BstNSI	RCATGY	4 cut(s)	1211, 3494, 3769, 4391
BstPI	GGTNACC	1 cut(s)	2032
BstSFI	CTRYAG	3 cut(s)	2733, 2982, 4890
BstSLI	GKGCMC	2 cut(s)	2087, 3562
BstUI	CGCG	3 cut(s)	207, 3792, 4183
BstV1I	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4945
BstV2I	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5224
BstXI	CCANNNNNNTGG	1 cut(s)	3875
Bsu36I	CCTNAGG	1 cut(s)	24
BsuI	GTATCC	1 cut(s)	4372
BsuRI	GGCC	7 cut(s)	245, 530, 1269, 1966, 3074, 3560, 5741
BtgI	CCRYGG	3 cut(s)	132, 736, 2116
BtgZI	GCGATG	1 cut(s)	4857
BtsI	GCAGTG	3 cut(s)	829, 2626, 3339
BtuMI	TCGCGA	1 cut(s)	3792
BveI	ACCTGC	1 cut(s)	4268
Cac8I	GCNNGC	8 cut(s)	326, 470, 1422, 2124, 3159, 4345, 4494, 5101
CaiI	CAGNNNCTG	2 cut(s)	1865, 3040
CfoI	GCGC	2 cut(s)	651, 3584
Cfr10I	RCCGGY	1 cut(s)	4273
CseI	GACGC	3 cut(s)	62, 477, 5708
CsiI	ACCWGGT	1 cut(s)	2949
Csp6I	GTAC	8 cut(s)	1374, 2288, 2436, 2727, 2765, 3113, 3508, 5387
CviQI	GTAC	8 cut(s)	1374, 2288, 2436, 2727, 2765, 3113, 3508, 5387
DraIII	CACNNNGTG	2 cut(s)	2322, 4340
DriI	GACNNNNNGTC	1 cut(s)	3053
Eam1104I	CTCTTC	6 cut(s)	847, 2506, 2872, 5139, 5561, 5727
Eam1105I	GACNNNNNGTC	1 cut(s)	3053
EarI	CTCTTC	6 cut(s)	847, 2506, 2872, 5139, 5561, 5727
EciI	GGCGGA	1 cut(s)	236
Ecl136II	GAGCTC	1 cut(s)	186
Eco147I	AGGCCT	1 cut(s)	530
Eco24I	GRGCYC	3 cut(s)	188, 326, 3562
Eco31I	GGTCTC	1 cut(s)	2903
Eco47I	GGWCC	8 cut(s)	123, 442, 703, 2638, 3196, 3268, 3863, 4729
Eco47III	AGCGCT	1 cut(s)	3583
Eco53kI	GAGCTC	1 cut(s)	186
Eco57I	CTGAAG	4 cut(s)	1356, 2211, 2238, 3304
Eco72I	CACGTG	1 cut(s)	4483
Eco81I	CCTNAGG	1 cut(s)	24
Eco88I	CYCGRG	1 cut(s)	1122
Eco91I	GGTNACC	1 cut(s)	2032
EcoICRI	GAGCTC	1 cut(s)	186
EcoNI	CCTNNNNNAGG	1 cut(s)	27
EcoO109I	RGGNCCY	3 cut(s)	442, 3558, 4729
EcoO65I	GGTNACC	1 cut(s)	2032
EcoRI	GAATTC	3 cut(s)	236, 1392, 4249
EcoT22I	ATGCAT	2 cut(s)	2907, 2995
EcoT38I	GRGCYC	3 cut(s)	188, 326, 3562
Esp3I	CGTCTC	4 cut(s)	45, 1930, 4212, 5120
FalI	AAGNNNNNCTT	4 cut(s)	2382, 2414, 4806, 4838
FaqI	GGGAC	3 cut(s)	143, 3849, 4742
FauI	CCCGC	3 cut(s)	875, 3150, 5376
FauNDI	CATATG	7 cut(s)	882, 1312, 1584, 1732, 2862, 3870, 4432
FbaI	TGATCA	2 cut(s)	2167, 5596
FblI	GTMKAC	1 cut(s)	4833
FriOI	GRGCYC	3 cut(s)	188, 326, 3562
GlaI	GCGC	2 cut(s)	650, 3583
GsaI	CCCAGC	2 cut(s)	2003, 3565
GsuI	CTGGAG	2 cut(s)	5193, 5519
HaeII	RGCGCY	1 cut(s)	3585
HaeIII	GGCC	7 cut(s)	245, 530, 1269, 1966, 3074, 3560, 5741
HapII	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4274
HgaI	GACGC	3 cut(s)	62, 477, 5708
HhaI	GCGC	2 cut(s)	651, 3584
Hin1I	GRCGYC	1 cut(s)	156
Hin6I	GCGC	2 cut(s)	649, 3582
HinP1I	GCGC	2 cut(s)	649, 3582
HincII	GTYRAC	7 cut(s)	802, 830, 1522, 2026, 2797, 3426, 3537
HindII	GTYRAC	7 cut(s)	802, 830, 1522, 2026, 2797, 3426, 3537
HindIII	AAGCTT	2 cut(s)	1349, 4187
HpaI	GTTAAC	2 cut(s)	3426, 3537
HpaII	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4274
HphI	GGTGA	2 cut(s)	2005, 2105
Hpy99I	CGWCG	4 cut(s)	13, 158, 212, 3716
HpyCH4IV	ACGT	5 cut(s)	156, 1789, 4482, 5537, 5748
HpySE526I	ACGT	5 cut(s)	156, 1789, 4482, 5537, 5748
Hsp92I	GRCGYC	1 cut(s)	156
HspAI	GCGC	2 cut(s)	649, 3582
Kpn2I	TCCGGA	1 cut(s)	561
KpnI	GGTACC	1 cut(s)	2730
Ksp22I	TGATCA	2 cut(s)	2167, 5596
KspAI	GTTAAC	2 cut(s)	3426, 3537
LguI	GCTCTTC	1 cut(s)	2506
LmnI	GCTCC	9 cut(s)	61, 183, 1201, 1855, 2245, 2750, 4297, 4942, 5716
Lsp1109I	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4945
MabI	ACCWGGT	1 cut(s)	2949
MaeII	ACGT	5 cut(s)	156, 1789, 4482, 5537, 5748
MfeI	CAATTG	4 cut(s)	596, 942, 3480, 4449
MhlI	GDGCHC	6 cut(s)	188, 326, 2087, 2790, 3562, 5076
MlyI	GAGTC	5 cut(s)	1827, 3140, 3899, 4436, 5298
MmeI	TCCRAC	7 cut(s)	102, 1870, 3610, 3942, 4239, 4372, 4450
Mph1103I	ATGCAT	2 cut(s)	2907, 2995
MroI	TCCGGA	1 cut(s)	561
MroXI	GAANNNNTTC	2 cut(s)	1341, 5231
MslI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4755, 5412
MspA1I	CMGCKG	2 cut(s)	574, 3137
MspI	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4274
MunI	CAATTG	4 cut(s)	596, 942, 3480, 4449
Mva1269I	GAATGC	1 cut(s)	3778
MvnI	CGCG	3 cut(s)	207, 3792, 4183
NciI	CCSGG	3 cut(s)	960, 2030, 2525
NcoI	CCATGG	3 cut(s)	132, 736, 2116
NdeI	CATATG	7 cut(s)	882, 1312, 1584, 1732, 2862, 3870, 4432
NmeAIII	GCCGAG	2 cut(s)	271, 1896
NmuCI	GTSAC	9 cut(s)	916, 1229, 2347, 3337, 3493, 4332, 4816, 5481, 5720
NruI	TCGCGA	1 cut(s)	3792
NsiI	ATGCAT	2 cut(s)	2907, 2995
NspI	RCATGY	4 cut(s)	1211, 3494, 3769, 4391
OliI	CACNNNNGTG	1 cut(s)	1765
PaeR7I	CTCGAG	1 cut(s)	1122
PasI	CCCWGGG	1 cut(s)	398
PceI	AGGCCT	1 cut(s)	530
PciI	ACATGT	2 cut(s)	1207, 3490
PciSI	GCTCTTC	1 cut(s)	2506
PcsI	WCGNNNNNNNCGW	2 cut(s)	153, 159
PctI	GAATGC	1 cut(s)	3778
PdmI	GAANNNNTTC	2 cut(s)	1341, 5231
PflFI	GACNNNGTC	1 cut(s)	155
PflMI	CCANNNNNTGG	3 cut(s)	2122, 3839, 3929
PfoI	TCCNGGA	3 cut(s)	2523, 2640, 5496
PleI	GAGTC	5 cut(s)	1826, 3139, 3899, 4435, 5297
PmaCI	CACGTG	1 cut(s)	4483
PmlI	CACGTG	1 cut(s)	4483
PpsI	GAGTC	5 cut(s)	1826, 3139, 3899, 4435, 5297
Ppu21I	YACGTR	2 cut(s)	4483, 5749
PpuMI	RGGWCCY	2 cut(s)	442, 4729
PscI	ACATGT	2 cut(s)	1207, 3490
PshBI	ATTAAT	2 cut(s)	1754, 2232
Psp124BI	GAGCTC	1 cut(s)	188
Psp5II	RGGWCCY	2 cut(s)	442, 4729
PspCI	CACGTG	1 cut(s)	4483
PspEI	GGTNACC	1 cut(s)	2032
PspFI	CCCAGC	2 cut(s)	1999, 3561
PspOMI	GGGCCC	1 cut(s)	3558
PspPPI	RGGWCCY	2 cut(s)	442, 4729
PspXI	VCTCGAGB	1 cut(s)	1122
PstNI	CAGNNNCTG	2 cut(s)	1865, 3040
PsyI	GACNNNGTC	1 cut(s)	155
PvuII	CAGCTG	1 cut(s)	574
RruI	TCGCGA	1 cut(s)	3792
RsaI	GTAC	8 cut(s)	1375, 2289, 2437, 2728, 2766, 3114, 3509, 5388
RsaNI	GTAC	8 cut(s)	1374, 2288, 2436, 2727, 2765, 3113, 3508, 5387
RseI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4755, 5412
SacI	GAGCTC	1 cut(s)	188
SapI	GCTCTTC	1 cut(s)	2506
ScaI	AGTACT	1 cut(s)	2437
SchI	GAGTC	5 cut(s)	1827, 3140, 3899, 4436, 5298
SduI	GDGCHC	6 cut(s)	188, 326, 2087, 2790, 3562, 5076
SexAI	ACCWGGT	1 cut(s)	2949
SfcI	CTRYAG	3 cut(s)	2733, 2982, 4890
Sfr274I	CTCGAG	1 cut(s)	1122
SinI	GGWCC	8 cut(s)	123, 442, 703, 2638, 3196, 3268, 3863, 4729
SlaI	CTCGAG	1 cut(s)	1122
SmiMI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4755, 5412
SmlI	CTYRAG	3 cut(s)	929, 1122, 1293
SmoI	CTYRAG	3 cut(s)	929, 1122, 1293
SpeI	ACTAGT	3 cut(s)	478, 5017, 5654
SseBI	AGGCCT	1 cut(s)	530
SsiI	CCGC	8 cut(s)	117, 221, 868, 3135, 3157, 4183, 4880, 5383
SspI	AATATT	3 cut(s)	1066, 3454, 5548
SstI	GAGCTC	1 cut(s)	188
StuI	AGGCCT	1 cut(s)	530
TaiI	ACGT	5 cut(s)	159, 1792, 4485, 5540, 5751
TaqII	GACCGA	1 cut(s)	443
TatI	WGTACW	4 cut(s)	2287, 2435, 2764, 3507
TauI	GCSGC	1 cut(s)	119
TseFI	GTSAC	9 cut(s)	916, 1229, 2347, 3337, 3493, 4332, 4816, 5481, 5720
TseI	GCWGC	9 cut(s)	142, 571, 842, 1742, 1794, 2004, 2126, 2560, 4958
Tsp45I	GTSAC	9 cut(s)	916, 1229, 2347, 3337, 3493, 4332, 4816, 5481, 5720
TspGWI	ACGGA	5 cut(s)	643, 1637, 3260, 4105, 4109
Tth111I	GACNNNGTC	1 cut(s)	155
Van91I	CCANNNNNTGG	3 cut(s)	2122, 3839, 3929
VpaK11BI	GGWCC	8 cut(s)	123, 442, 703, 2638, 3196, 3268, 3863, 4729
VspI	ATTAAT	2 cut(s)	1754, 2232
XagI	CCTNNNNNAGG	1 cut(s)	27
XbaI	TCTAGA	1 cut(s)	4790
XceI	RCATGY	4 cut(s)	1211, 3494, 3769, 4391
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1811, 2047, 3181
XhoI	CTCGAG	1 cut(s)	1122
XmaJI	CCTAGG	1 cut(s)	4991
XmiI	GTMKAC	1 cut(s)	4833
XmnI	GAANNNNTTC	2 cut(s)	1341, 5231
ZraI	GACGTC	1 cut(s)	157
ZrmI	AGTACT	1 cut(s)	2437
Zsp2I	ATGCAT	2 cut(s)	2907, 2995

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.