Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0006835

Physical Location & Seq

Genomic Coordinates Forward (+)

26288620 .. 26305221

Gene Structure 16602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023635

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5790 bp

ATGGCACCGTCGTCCAAGAAACCTCAGGGAGGGAATAACAAGAATCCCAGAGACGCCCCAAAGTTCAACAAATCCTCCAAAAAACCTTTCAAACCAAACAAGGATCACAACAACGCCGCCAGGTCCGAAGCCATGGCTTTGCAGCTCGAAGACGACGTCCCTGACTTCCCCAGAGGTGGAGGGAGCTCATTGAATCGAAAAGAACGCGACGAAATTCGGGCGGAAGTTGATGCCGAATTCGAGGCAGAGGAGAGGGAGATGAAGAAGAAAGGGAAGAAGAAAAGGGTGCCGAAGGAGAGCCTTGTAAACGAGGACGAGATGGGCTCGCTTTTCGGCGATGGCATCACTGGAAAACTACCTAGATATGCAAACAAGATCACTTTGAAGAATATATCTCCTGGCATGAAGGTTTGGGGAGTTGTTGCCGAAGTCAATGAAAAGGACCTTGTAATTAGTCTTCCAGGGGGTTTGCGTGGACTAGTTCGATCGTCCGATGCTTTTGATCCTATTCTGGATGATGAAACTGAGGCCTTAGCAGATAGTGCCCTCCCTAGCATATTCCGGATAGGGCAGCTGGTATCTTGCATTGTGTTGCAATTGGATGACGATAAGAAAGAGAAAGGGAAACGGAAGATCTGGCTTTCTTTGCGCCTCTCTTTGTTGCATAAAGGCTTCACTTTGGATTCTGTTCAAGAAGGCATGGTCCTTACAGCCTATATTAAAAGCATCGAAGACCATGGTTACATTCTTCATTTTGGTTTATCTTCATTCACAGGGTTTTTGCCAAAAAATAGCCAAGCTGACAGCATGGTTCAAGTGAGCAGTGGTCAACTGCTTCAAGCTGCTGTTAGAAGAGTTGATAAAACCCGCAAGGTTATTCATATGAGTTCTGACCCAGAGATGGTGTCGAAGTGTGTGACCAAAGATCTCAAGGGAATTTCGATTGATCTACTTGTTCCGGGGATGATGGTCAATGCTCGTGTGTTATCCACCCTTGAAAATGGGGTTATGTTATCATTTCTTACTTATTTTACTGGAACAGTTGATATATTTCATCTGCAAAATATTTACCCTACTACAAACTGGAAGGAAGATTACAATCACAACAAGAAGCTCAATGCTCGAGTATTATTTGTCGATCCTTCAACTAGAGCTGTTGGTTTGACACTAAATCCACATCTTGTTCGCAACAAGGCTCCTCCTTCACATGTCAAAATTGGAGATATATGTGACGATGCAAAGGTAGTGAGGGTTGATAGAGGATTGGGCCTTCTGCTTGAAATACCATCTACTCAAGTTTCAACTCCGGCATACGTTAGTATATCTGATGTGGCTGAAGAAGTTCGGAAGCTTGAAAAGAAGTTCAAGGAAGGTACTCGTGTTCGTGTCAGAATTCTTGGGTTTAGGCACTTGGAAGGGCTTGCCACAGGGATTTTGAAGGCAAGTGCATTTGAAGGGTCAGTTTTCACTCACTCTGATGTCAAACCTGGGATGGTTGTGAGAGGTAAAATAATAGCTGTTGACAGCTTTGGGGCTATCGTGCAATTCCCTGGTGGTGTGAAGGCTCTTTGTCCTCTTACTCATATGTCTGAATTTGAAATTGCAAAGCCCAGAAAAAAATTTAAGGTTGGGGCTGAACTAGTGTTCCGTGTGCTTGGTTGCAAGTCCAAGAGAATAACTGTTACACACAAGAAAACACTTGTAAAGTCAAAACTGGGGATTCTTAGTTCATATGCTGATGCTGCTGATGGATTAATAACACATGGGTGGATAAGAAAGATTGAAGAACGTGGCTGCTTTGTCCATTTTTACAATGGAGTCCAAGGATTTGCCCCAAGATCTGAACTCGGATTGGAGCCAGGATCGGACCCAAGTTCAATGTATCATGTTGGACAAGTTATCAAATGTAGAGTTATTGGCTCTAATGCTACATCGAGACGAATCAAACTTAGCTTGATAATACGGCCTCCAAGGGTTTCTGAGGATGATATGGCGAAGTTGGGCAGCCTGGTTTCAGGAGTTGTTGACCGGGTAACCCCGAATGCTGTCATGGTTTATGTTAATGCAAAAGGTTACTCAATGGGCACAATTTTTACAGACCACTTGGCAGATCACCATGGGCTGGCTGCTTTGATGAAGTCAGTATTGAAGCCTGGTTATGAATTTGATCAATTATTGGTTTTAGACACTGAAGGCAACAATTTGATCCTATCTGCAAAACATTCTCTGATTAATTCAGCTCCCATGCTTCCTTCAGAAGTCAGTCAAGTTCATCCTAATACGGTAGTACATGGCTACATTTGTAACTTAATTGACACTGGGTGCTTTGTTCGCTTTCTTGGGCGTGTGACTGGTTTCTCTCCAAGGCACAAGGCAATGGATGACCATAAAGGGGATCTTTCAGAAGCCTATTACATTGGACAGTCTGTTCGGAGTACTATACTCGATGTCAATAGTGAAACAGGTAGGATAACATTATCATTGAAGCAGTCATCTTGCTCTTCAACAGATGCCTCCTTTATCCGGGAATACTTTATTTCAGAGGAGAAGATTGCAAAGCTGCAAGCGTTGGATTCAAAAGAATCCAAATCAGATTGGTCAGAAGGATTTTCCATTGGCAGTGTTGTTGAAGGGAAGGTCCAGGAGGCAAAGGACATTGGAGTTGTTGTCAGCTTTGAGAAATATAATGATGTTTTCGGTTTCATCACGCACTATCAATTAGCTGGTACCACTGTAGAAACAGGCTCCATTGTTCGAGCAGTTGTACTTGATGTTGCCAAGGCAGAGCACCTTGTTGACTTATCACTCAAACCAGAATTCATTACTAACTTAAAACAAGAAAGCTCTAAGAGCCAAACTCATATGAAGAAACGAAGAAGAGAAGTCTCGGAGGATCCAGAGATGCATGAGACCGTAAATGCTGTGGTTGAGATTGTGAAGGAGAATTACCTGGTTCTTTCTATCCCAAAGTATAACCATGCTGTAGGATATGCATCAATTTCTGACTACAACACTCAAAAGTTTCCTCAGAAACAGTTTCTGAATGGACAAAGTGTCAATGCTACTGTCATGGCCCTTCCAAGCCCTACAACCGTTGGGAGATTGCTTTTGCTACTTAATTCTCTAAGTGAGTCCTCTGATACTTCCAGTTCAAAGCGGGCAAAGAAGAAATCCAGCTACAAAGTGGGATCTGTGGTCCAGGCAGAGATAACTGAAATAAAGGCTCTTGAACTAAGATTGAAGTTTGGAATCGGCTTTCATGGACGGGTCCGTATAACAGAGGTAAATGATGATCTGTTGGAAGATCCATTTAACAACTTCAGAATTGGGCAAACAGTCACTGCAAGGATTGTTGCAAAGACTAATTATTCAGATAACAACAAAAAGAGTTTCCAGTGGGATCTGTCTTTGAAACCTAGTTTGTTAACAGGTTCCTGTGAAATAGAGGAAAATATTTTGAAAGGAGATCTCGATTTCTCAATTGGAAAACATGTCACTGGCTATGTGTACAAGGTGGATGCTGAATGGGTCTGGTTAACAATATCTCGAAATGTTAGGGCTCAGCTTTTCATTCTTGACAGCGCTTGTAAGCCTAGTGAACTTCAAGAGTTTCAGAAACGCTTTCATGTGGGAAATGCTGTATCTGGTCATGTTTTAAGTGTTAGCAAGGAGAAAAAGCTATTACGGCTGGTTTCATATCCTTTTACTCCTGTCGCTGATAAAGCTGTTGATAATGAGGTGTCAAAGACGGATGCAAATGTTAGCATGTTGAATGCAACTGCTCATATTCGCGAGGGATGTGTTATAGGTGGGAGAATTATTAAAAAACTTCCAGGTGTTGGTGGATTGACTGTGCAAATTGGTCCCCATATGTATGGCAGGGTTCATTATAGTGAGCTTTCTGACTCATGGGTGTCCAACCCATTGTCTGGATATGAGGAAGGTCAGTTTGTCAAATGCAAGGTCGTTGAATGTAGTCGCTCTGGACAGGGAACTTTTCACATTGACTTGTCATTACGGTCTTCTTTAGTGGGCACACTTTGTCAAGATTCCAATGAACCTGACAATGACAAGCATACTCATACCGAACGGCCGGAAAAGATTGACGATCTGAAACCTAATATGGTCATACAGGGTTATGTGAAAAATGTTTCATCAAAAGGATGTTTTATTTTGCTTTCGCGGAAGCTTGATGCCAAAGTTCTCGTCTCCAATTTATCCGATGGATATGTTGATGATCCTGAAAAGGAATTCCCAGTTGGAAAACTTGTTACTGGCAGGGTGTCATCTGTGGAGCCTTTATCAAAGAGAGTTGAAGTTACCTTGAAGTCACTGAGTGCAAGCAGTTTGCCACAATCTGCAAGGAATAATTTGGATAGCTTGCATGTCGGAGATATTATCTCTGGCAGGGTAAAACGAGTTGAGTCATATGGATTATTCATCACAATTGACAATACAAATGTGGTTGGATTATGCCACGTGTCAGAGCTTGCAGAAGATAAGAAGGACAATTTTGAAACTAAATACAAAACAGGAGAGAGGGTGTCAGCAAAAGTATTGAAGGTGGACAAGGAGAGGCACCGAGTTTCTCTCGGAATGAAGGATTTGTATATTATGGATAGCGACCACACACCTCCAAAACAAGATCTTGATGAACCTATCAGACAGACTGATTTGGTGGATGATTCTAAGTCAGTTATGTGCCCTGTTGGCAGTTTCCTTGGGGACCAGAACATGGATATTGAACACGAAAATGCAGAGTTCCAGTTTCTTGCACAAGCAGAATCAAGAGCTTTAGTTCCTCCACTTGAAGTCACTCTTGACGATGTAGACCAAGGCAATGATATTGTTAGCCAGGATCAAGAGCTTCCTGATGCGGAGGATGCTGTAGATGACAAGAAAAAGAAGCTCACGAAGAAAAATGCCAGGGATGAGAGGGAGCGTGAGATAAGGGCTGCTGAGGAAAGATTATTGGAGAAAGATATACCTAGGACTGATGAGGAATTTGAGAAACTAGTTAGAAGTTCTCCAAATAACAGTTATGTTTGGATAAAATATATGGAGTTTGTGCTCTCTATGGCTGATGTTGAGAAGGCACGCTCTATTGCTAAAAGGGCTCTGGAGACGATAAATTTTCGAGAAGAGAATGAGAAGCTCAATGTTTGGGTGGCTTATTTTAATTTGGAAAGCAAATATGGAAGTCCTCCAGAGGAGGCTGTAATGAAAGTCTTCCAAAAAGCGTTGCAGTATAATGATCCCAAAAGGGTTCATCTAGCACTCTTGGGAGTCCTTGAGAGGTCTGAGCAACACAAGTTGGCTGATGAACTTGCTGATAACATGATTAAGAGGTTTAAGAAGTCTTGCAAGGTTTGGCTAAGGCGGGTACAGAGGCTTTTGGTGCAACAGCAGGATGGGGTTCAGGATTTTGTCTCTCGTGCTGAAAAGATCCTTCCTAAGCATAAGCATATCAAATTCTTGTCACAGACAGCCATCTTGGAGTTCAAATGTGGGAATCCAGAGAGAGGACGCTCCATGTTTGAAAATATTTTGAGGCAAAACCCGAAGAGAACAGACTTGTGGAGTGTTTATCTTGATCAAGAAATTCGACTTGGAGATACTGATCTGATTCATGCATTGTTTGAGAGGGCAACTAGTTTGAGCCTACCAGCTAAAAAGATGAAATTCTTGTTCAAAAAATATCTGGAATATGAGGAGCGTCATGGTGATGAAGAGAGGGCCAATTACGTGAAACAGAAGGCAATGAGTTATGTTGAGAATACAGTAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0000462 GO:0000466 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009553 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

S1 Suf

Protein Analysis

1930

Amino Acids

215.49

Weight (kDa)

8.63

Isoelectric Point (pI)

37.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1_RRP5	PF23459	132 - 205	8.3e-06	RRP5 S1 domain
S1_RRP5	PF23459	319 - 390	1.4e-06	RRP5 S1 domain
OB_RRP5_4th	PF24685	399 - 489	4.2e-40	RRP5 OB-fold domain
S1	PF00575	494 - 558	3.8e-08	S1 RNA binding domain
S1	PF00575	588 - 652	4.1e-06	S1 RNA binding domain
S1	PF00575	759 - 829	1.9e-07	S1 RNA binding domain
OB_RRP5	PF24682	954 - 1041	1.2e-38	RRP5 OB-fold domain
S1_RRP5	PF23459	1371 - 1441	2.3e-07	RRP5 S1 domain
S1	PF00575	1463 - 1534	2.6e-16	S1 RNA binding domain
HAT_Syf1_CNRKL1_C	PF23231	1677 - 1926	3.7e-10	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
Suf	PF05843	1827 - 1925	1.9e-06	Suppressor of forked protein (Suf)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11964 AT3G11964
fragaria_vesca	FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470
malus_domestica	MD12G1051100.v1.1
prunus_persica	Prupe.7G085700_v2.0.a1 Prupe.7G085700_v2.0.a1
pyrus_communis	pycom12g04550
rosa_chinensis	RchiOBHm_Chr3g0478661
rosa_laevigata	RLG00000023635
rosa_multiflora	Rmu_sc0002454.1_g000002 Rmu_sc0023704.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403220
rosa_rugosa	Rorug03G0166800 Rorug03G0166800 Rorug03G0166900
rosa_samantha	Rh3AG219000 Rh3BG250700 Rh3CG247500 Rh3DG243800
rosa_wichuraiana	Rw3G019690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	159
Acc65I	GGTACC	1 cut(s)	2726
AccB1I	GGYRCC	4 cut(s)	4, 286, 2726, 4578
AccB7I	CCANNNNNTGG	4 cut(s)	2122, 3839, 3929, 4735
AccI	GTMKAC	1 cut(s)	4830
AccII	CGCG	3 cut(s)	207, 3792, 4183
AccIII	TCCGGA	1 cut(s)	561
AciI	CCGC	7 cut(s)	117, 221, 868, 3157, 4183, 4877, 5380
AcoI	YGGCCR	1 cut(s)	4091
AcuI	CTGAAG	4 cut(s)	1356, 2211, 2238, 3304
AcvI	CACGTG	1 cut(s)	4480
AcyI	GRCGYC	2 cut(s)	54, 156
AdeI	CACNNNGTG	2 cut(s)	2322, 4337
AfaI	GTAC	7 cut(s)	1375, 2289, 2437, 2728, 2766, 3509, 5385
AfeI	AGCGCT	1 cut(s)	3583
AflIII	ACRYGT	3 cut(s)	1207, 3490, 4479
AhdI	GACNNNNNGTC	1 cut(s)	3053
AhlI	ACTAGT	4 cut(s)	478, 1639, 5014, 5651
AjuI	GAANNNNNNNTTGG	6 cut(s)	71, 103, 4242, 4274, 5592, 5624
AleI	CACNNNNGTG	1 cut(s)	1765
AloI	GAACNNNNNNTCC	6 cut(s)	1629, 1661, 1711, 1743, 2412, 2444
Alw21I	GWGCWC	3 cut(s)	188, 2790, 5073
Alw26I	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4213, 5117, 5434
AlwNI	CAGNNNCTG	2 cut(s)	3040, 3341
Ama87I	CYCGRG	1 cut(s)	1122
Aor13HI	TCCGGA	1 cut(s)	561
Aor51HI	AGCGCT	1 cut(s)	3583
AoxI	GGCC	6 cut(s)	528, 1267, 1964, 3072, 4091, 5736
ApeKI	GCWGC	9 cut(s)	142, 571, 842, 1742, 1794, 2004, 2126, 2560, 4955
ArsI	GACNNNNNNTTYG	8 cut(s)	141, 173, 2412, 2444, 3948, 3980, 4191, 4223
AseI	ATTAAT	2 cut(s)	1754, 2232
Asp700I	GAANNNNTTC	2 cut(s)	1341, 5228
Asp718I	GGTACC	1 cut(s)	2726
AspA2I	CCTAGG	1 cut(s)	4988
AspLEI	GCGC	2 cut(s)	651, 3584
AsuC2I	CCSGG	3 cut(s)	960, 2030, 2525
AsuHPI	GGTGA	2 cut(s)	2105, 5735
AvaI	CYCGRG	1 cut(s)	1122
AvaII	GGWCC	9 cut(s)	123, 442, 703, 1867, 2638, 3196, 3268, 3863, 4726
AvrII	CCTAGG	1 cut(s)	4988
AxyI	CCTNAGG	1 cut(s)	24
BaeGI	GKGCMC	4 cut(s)	547, 2087, 4037, 4706
BaeI	ACNNNNGTAYC	4 cut(s)	1365, 1398, 2718, 2751
BamHI	GGATCC	1 cut(s)	2893
BanI	GGYRCC	4 cut(s)	4, 286, 2726, 4578
BanII	GRGCYC	4 cut(s)	188, 326, 3562, 5119
BarI	GAAGNNNNNNTAC	6 cut(s)	441, 473, 1079, 1111, 2930, 2962
BauI	CACGAG	3 cut(s)	978, 1377, 5433
BbrPI	CACGTG	1 cut(s)	4480
BbsI	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5221
Bbv12I	GWGCWC	3 cut(s)	188, 2790, 5073
BbvCI	CCTCAGC	1 cut(s)	4959
BbvI	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4942
BceAI	ACGGC	3 cut(s)	1979, 3701, 4106
BclI	TGATCA	2 cut(s)	2167, 5593
BcnI	CCSGG	3 cut(s)	960, 2030, 2525
BcoDI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4213, 5117, 5434
BcuI	ACTAGT	4 cut(s)	478, 1639, 5014, 5651
BfmI	CTRYAG	3 cut(s)	2733, 2982, 4887
BfoI	RGCGCY	1 cut(s)	3585
BglI	GCCNNNNNGGC	1 cut(s)	4710
BglII	AGATCT	5 cut(s)	633, 925, 1838, 3466, 4645
BlnI	CCTAGG	1 cut(s)	4988
BlpI	GCTNAGC	1 cut(s)	3561
BmcAI	AGTACT	1 cut(s)	2437
Bme18I	GGWCC	9 cut(s)	123, 442, 703, 1867, 2638, 3196, 3268, 3863, 4726
BmeRI	GACNNNNNGTC	1 cut(s)	3053
BmeT110I	CYCGRG	1 cut(s)	1122
BmrI	ACTGGG	3 cut(s)	1724, 2328, 4250
BmuI	ACTGGG	3 cut(s)	1724, 2328, 4250
BpiI	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5221
BplI	GAGNNNNNCTC	6 cut(s)	308, 340, 2842, 2874, 4575, 4607
BpmI	CTGGAG	2 cut(s)	5141, 5190
Bpu10I	CCTNAGC	4 cut(s)	532, 4959, 5375, 5454
Bpu1102I	GCTNAGC	1 cut(s)	3561
BpuEI	CTTGAG	3 cut(s)	914, 1278, 5312
BpuMI	CCSGG	3 cut(s)	960, 2030, 2525
BsaAI	YACGTR	2 cut(s)	4480, 5746
BsaBI	GATNNNNATC	3 cut(s)	1098, 2992, 5619
BsaHI	GRCGYC	2 cut(s)	54, 156
BsaI	GGTCTC	1 cut(s)	2903
BsaWI	WCCGGW	1 cut(s)	561
BsaXI	ACNNNNNCTCC	6 cut(s)	59, 89, 2652, 2682, 4527, 4557
Bse21I	CCTNAGG	1 cut(s)	24
Bse3DI	GCAATG	3 cut(s)	2382, 4846, 5766
Bse8I	GATNNNNATC	3 cut(s)	1098, 2992, 5619
BseAI	TCCGGA	1 cut(s)	561
BseJI	GATNNNNATC	3 cut(s)	1098, 2992, 5619
BseMI	GCAATG	3 cut(s)	2382, 4846, 5766
BseMII	CTCAG	8 cut(s)	38, 516, 1971, 3041, 3575, 4325, 4950, 5292
BseRI	GAGGAG	5 cut(s)	263, 1188, 2558, 5225, 5726
BseSI	GKGCMC	4 cut(s)	547, 2087, 4037, 4706
BseX3I	CGGCCG	1 cut(s)	4091
BseXI	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4942
Bsh1236I	CGCG	3 cut(s)	207, 3792, 4183
Bsh1285I	CGRYCG	2 cut(s)	488, 4094
BshFI	GGCC	6 cut(s)	530, 1269, 1966, 3074, 4093, 5738
BshNI	GGYRCC	4 cut(s)	4, 286, 2726, 4578
BsiEI	CGRYCG	2 cut(s)	488, 4094
BsiHKAI	GWGCWC	3 cut(s)	188, 2790, 5073
BsiHKCI	CYCGRG	1 cut(s)	1122
BsiSI	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4094
BslFI	GGGAC	3 cut(s)	143, 3849, 4739
BsmAI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4213, 5117, 5434
BsmBI	CGTCTC	4 cut(s)	45, 1930, 4213, 5117
BsmFI	GGGAC	3 cut(s)	143, 3849, 4739
BsmI	GAATGC	2 cut(s)	2047, 3778
BsnI	GGCC	6 cut(s)	530, 1269, 1966, 3074, 4093, 5738
Bso31I	GGTCTC	1 cut(s)	2903
BsoBI	CYCGRG	1 cut(s)	1122
Bsp13I	TCCGGA	1 cut(s)	561
Bsp1407I	TGTACA	1 cut(s)	3507
Bsp1720I	GCTNAGC	1 cut(s)	3561
Bsp19I	CCATGG	3 cut(s)	132, 736, 2116
Bsp68I	TCGCGA	1 cut(s)	3792
BspACI	CCGC	7 cut(s)	117, 221, 868, 3157, 4183, 4877, 5380
BspANI	GGCC	6 cut(s)	530, 1269, 1966, 3074, 4093, 5738
BspCNI	CTCAG	8 cut(s)	37, 517, 1972, 3040, 3574, 4326, 4951, 5293
BspEI	TCCGGA	1 cut(s)	561
BspFNI	CGCG	3 cut(s)	207, 3792, 4183
BspQI	GCTCTTC	1 cut(s)	2506
BspT107I	GGYRCC	4 cut(s)	4, 286, 2726, 4578
BspTNI	GGTCTC	1 cut(s)	2903
BsrDI	GCAATG	3 cut(s)	2382, 4846, 5766
BsrGI	TGTACA	1 cut(s)	3507
BssNI	GRCGYC	2 cut(s)	54, 156
BssSI	CACGAG	3 cut(s)	978, 1377, 5433
Bst2BI	CACGAG	3 cut(s)	978, 1377, 5433
Bst6I	CTCTTC	6 cut(s)	847, 2506, 2872, 5136, 5558, 5724
BstACI	GRCGYC	2 cut(s)	54, 156
BstAPI	GCANNNNNTGC	1 cut(s)	542
BstAUI	TGTACA	1 cut(s)	3507
BstBAI	YACGTR	2 cut(s)	4480, 5746
BstC8I	GCNNGC	9 cut(s)	326, 1422, 2124, 2565, 3159, 4342, 4382, 4491, 5098
BstDSI	CCRYGG	3 cut(s)	132, 736, 2116
BstEII	GGTNACC	1 cut(s)	2032
BstENI	CCTNNNNNAGG	1 cut(s)	27
BstFNI	CGCG	3 cut(s)	207, 3792, 4183
BstH2I	RGCGCY	1 cut(s)	3585
BstHHI	GCGC	2 cut(s)	651, 3584
BstMAI	GTCTC	7 cut(s)	45, 1930, 2890, 2903, 4213, 5117, 5434
BstMCI	CGRYCG	2 cut(s)	488, 4094
BstNSI	RCATGY	4 cut(s)	1211, 3494, 3769, 4388
BstPI	GGTNACC	1 cut(s)	2032
BstSFI	CTRYAG	3 cut(s)	2733, 2982, 4887
BstSLI	GKGCMC	4 cut(s)	547, 2087, 4037, 4706
BstUI	CGCG	3 cut(s)	207, 3792, 4183
BstV1I	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4942
BstV2I	GAAGAC	5 cut(s)	156, 449, 738, 4014, 5221
BstXI	CCANNNNNNTGG	1 cut(s)	3875
BstZI	CGGCCG	1 cut(s)	4091
Bsu36I	CCTNAGG	1 cut(s)	24
BsuRI	GGCC	6 cut(s)	530, 1269, 1966, 3074, 4093, 5738
BtgI	CCRYGG	3 cut(s)	132, 736, 2116
BtgZI	GCGATG	1 cut(s)	351
BtsI	GCAGTG	3 cut(s)	829, 2626, 3339
BtuMI	TCGCGA	1 cut(s)	3792
Cac8I	GCNNGC	9 cut(s)	326, 1422, 2124, 2565, 3159, 4342, 4382, 4491, 5098
CaiI	CAGNNNCTG	2 cut(s)	3040, 3341
CfoI	GCGC	2 cut(s)	651, 3584
CseI	GACGC	3 cut(s)	62, 5535, 5705
CsiI	ACCWGGT	1 cut(s)	2949
Csp6I	GTAC	7 cut(s)	1374, 2288, 2436, 2727, 2765, 3508, 5384
CviQI	GTAC	7 cut(s)	1374, 2288, 2436, 2727, 2765, 3508, 5384
DraIII	CACNNNGTG	2 cut(s)	2322, 4337
DriI	GACNNNNNGTC	1 cut(s)	3053
EaeI	YGGCCR	1 cut(s)	4091
EagI	CGGCCG	1 cut(s)	4091
Eam1104I	CTCTTC	6 cut(s)	847, 2506, 2872, 5136, 5558, 5724
Eam1105I	GACNNNNNGTC	1 cut(s)	3053
EarI	CTCTTC	6 cut(s)	847, 2506, 2872, 5136, 5558, 5724
EciI	GGCGGA	1 cut(s)	236
Ecl136II	GAGCTC	1 cut(s)	186
EclXI	CGGCCG	1 cut(s)	4091
Eco147I	AGGCCT	1 cut(s)	530
Eco24I	GRGCYC	4 cut(s)	188, 326, 3562, 5119
Eco31I	GGTCTC	1 cut(s)	2903
Eco47I	GGWCC	9 cut(s)	123, 442, 703, 1867, 2638, 3196, 3268, 3863, 4726
Eco47III	AGCGCT	1 cut(s)	3583
Eco52I	CGGCCG	1 cut(s)	4091
Eco53kI	GAGCTC	1 cut(s)	186
Eco57I	CTGAAG	4 cut(s)	1356, 2211, 2238, 3304
Eco72I	CACGTG	1 cut(s)	4480
Eco81I	CCTNAGG	1 cut(s)	24
Eco88I	CYCGRG	1 cut(s)	1122
Eco91I	GGTNACC	1 cut(s)	2032
EcoICRI	GAGCTC	1 cut(s)	186
EcoNI	CCTNNNNNAGG	1 cut(s)	27
EcoO109I	RGGNCCY	1 cut(s)	442
EcoO65I	GGTNACC	1 cut(s)	2032
EcoRI	GAATTC	4 cut(s)	236, 1392, 2816, 4250
EcoT22I	ATGCAT	3 cut(s)	2907, 2995, 5635
EcoT38I	GRGCYC	4 cut(s)	188, 326, 3562, 5119
Esp3I	CGTCTC	4 cut(s)	45, 1930, 4213, 5117
FalI	AAGNNNNNCTT	4 cut(s)	2382, 2414, 4803, 4835
FaqI	GGGAC	3 cut(s)	143, 3849, 4739
FauI	CCCGC	3 cut(s)	875, 3150, 5373
FauNDI	CATATG	6 cut(s)	882, 1584, 1732, 2862, 3870, 4429
FbaI	TGATCA	2 cut(s)	2167, 5593
FblI	GTMKAC	1 cut(s)	4830
FriOI	GRGCYC	4 cut(s)	188, 326, 3562, 5119
GlaI	GCGC	2 cut(s)	650, 3583
GsuI	CTGGAG	2 cut(s)	5141, 5190
HaeII	RGCGCY	1 cut(s)	3585
HaeIII	GGCC	6 cut(s)	530, 1269, 1966, 3074, 4093, 5738
HapII	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4094
HgaI	GACGC	3 cut(s)	62, 5535, 5705
HhaI	GCGC	2 cut(s)	651, 3584
Hin1I	GRCGYC	2 cut(s)	54, 156
Hin6I	GCGC	2 cut(s)	649, 3582
HinP1I	GCGC	2 cut(s)	649, 3582
HincII	GTYRAC	6 cut(s)	830, 1522, 2026, 2797, 3426, 3537
HindII	GTYRAC	6 cut(s)	830, 1522, 2026, 2797, 3426, 3537
HindIII	AAGCTT	2 cut(s)	1349, 4187
HpaI	GTTAAC	2 cut(s)	3426, 3537
HpaII	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4094
HphI	GGTGA	2 cut(s)	2105, 5735
Hpy99I	CGWCG	3 cut(s)	13, 158, 212
HpyCH4IV	ACGT	5 cut(s)	156, 1314, 1789, 4479, 5745
HpySE526I	ACGT	5 cut(s)	156, 1314, 1789, 4479, 5745
Hsp92I	GRCGYC	2 cut(s)	54, 156
HspAI	GCGC	2 cut(s)	649, 3582
Kpn2I	TCCGGA	1 cut(s)	561
KpnI	GGTACC	1 cut(s)	2730
Ksp22I	TGATCA	2 cut(s)	2167, 5593
KspAI	GTTAAC	2 cut(s)	3426, 3537
LguI	GCTCTTC	1 cut(s)	2506
LmnI	GCTCC	9 cut(s)	183, 1201, 1855, 2245, 2750, 4294, 4939, 5534, 5713
Lsp1109I	GCAGC	9 cut(s)	154, 583, 829, 1729, 1781, 2016, 2113, 2547, 4942
MabI	ACCWGGT	1 cut(s)	2949
MaeII	ACGT	5 cut(s)	156, 1314, 1789, 4479, 5745
MfeI	CAATTG	3 cut(s)	596, 3480, 4446
MlyI	GAGTC	5 cut(s)	1827, 3140, 3899, 4433, 5295
MmeI	TCCRAC	7 cut(s)	1870, 2550, 3279, 3942, 4240, 4369, 4447
Mph1103I	ATGCAT	3 cut(s)	2907, 2995, 5635
MroI	TCCGGA	1 cut(s)	561
MroXI	GAANNNNTTC	2 cut(s)	1341, 5228
MslI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4752, 5724
MspA1I	CMGCKG	1 cut(s)	574
MspI	CCGG	6 cut(s)	562, 959, 1307, 2029, 2524, 4094
MunI	CAATTG	3 cut(s)	596, 3480, 4446
Mva1269I	GAATGC	2 cut(s)	2047, 3778
MvnI	CGCG	3 cut(s)	207, 3792, 4183
NciI	CCSGG	3 cut(s)	960, 2030, 2525
NcoI	CCATGG	3 cut(s)	132, 736, 2116
NdeI	CATATG	6 cut(s)	882, 1584, 1732, 2862, 3870, 4429
NmuCI	GTSAC	8 cut(s)	916, 1229, 2347, 3337, 3493, 4329, 4813, 5478
NruI	TCGCGA	1 cut(s)	3792
NsiI	ATGCAT	3 cut(s)	2907, 2995, 5635
NspI	RCATGY	4 cut(s)	1211, 3494, 3769, 4388
OliI	CACNNNNGTG	1 cut(s)	1765
PaeR7I	CTCGAG	1 cut(s)	1122
PceI	AGGCCT	1 cut(s)	530
PciI	ACATGT	2 cut(s)	1207, 3490
PciSI	GCTCTTC	1 cut(s)	2506
PcsI	WCGNNNNNNNCGW	1 cut(s)	153
PctI	GAATGC	2 cut(s)	2047, 3778
PdmI	GAANNNNTTC	2 cut(s)	1341, 5228
PflFI	GACNNNGTC	1 cut(s)	155
PflMI	CCANNNNNTGG	4 cut(s)	2122, 3839, 3929, 4735
PfoI	TCCNGGA	2 cut(s)	2523, 2640
Ple19I	CGATCG	1 cut(s)	488
PleI	GAGTC	5 cut(s)	1826, 3139, 3899, 4432, 5294
PmaCI	CACGTG	1 cut(s)	4480
PmlI	CACGTG	1 cut(s)	4480
PpsI	GAGTC	5 cut(s)	1826, 3139, 3899, 4432, 5294
Ppu21I	YACGTR	2 cut(s)	4480, 5746
PpuMI	RGGWCCY	1 cut(s)	442
PscI	ACATGT	2 cut(s)	1207, 3490
PshBI	ATTAAT	2 cut(s)	1754, 2232
Psp124BI	GAGCTC	1 cut(s)	188
Psp5II	RGGWCCY	1 cut(s)	442
PspCI	CACGTG	1 cut(s)	4480
PspEI	GGTNACC	1 cut(s)	2032
PspPPI	RGGWCCY	1 cut(s)	442
PspXI	VCTCGAGB	1 cut(s)	1122
PstNI	CAGNNNCTG	2 cut(s)	3040, 3341
PsyI	GACNNNGTC	1 cut(s)	155
PvuI	CGATCG	1 cut(s)	488
PvuII	CAGCTG	1 cut(s)	574
RruI	TCGCGA	1 cut(s)	3792
RsaI	GTAC	7 cut(s)	1375, 2289, 2437, 2728, 2766, 3509, 5385
RsaNI	GTAC	7 cut(s)	1374, 2288, 2436, 2727, 2765, 3508, 5384
RseI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4752, 5724
SacI	GAGCTC	1 cut(s)	188
SapI	GCTCTTC	1 cut(s)	2506
ScaI	AGTACT	1 cut(s)	2437
SchI	GAGTC	5 cut(s)	1827, 3140, 3899, 4433, 5295
SexAI	ACCWGGT	1 cut(s)	2949
SfcI	CTRYAG	3 cut(s)	2733, 2982, 4887
Sfr274I	CTCGAG	1 cut(s)	1122
SinI	GGWCC	9 cut(s)	123, 442, 703, 1867, 2638, 3196, 3268, 3863, 4726
SlaI	CTCGAG	1 cut(s)	1122
SmiMI	CAYNNNNRTG	6 cut(s)	1476, 1765, 3873, 3891, 4752, 5724
SmlI	CTYRAG	4 cut(s)	929, 1122, 1293, 5291
SmoI	CTYRAG	4 cut(s)	929, 1122, 1293, 5291
SpeI	ACTAGT	4 cut(s)	478, 1639, 5014, 5651
SseBI	AGGCCT	1 cut(s)	530
SsiI	CCGC	7 cut(s)	117, 221, 868, 3157, 4183, 4877, 5380
SspI	AATATT	3 cut(s)	1066, 3454, 5545
SstI	GAGCTC	1 cut(s)	188
StuI	AGGCCT	1 cut(s)	530
TaiI	ACGT	5 cut(s)	159, 1317, 1792, 4482, 5748
TatI	WGTACW	4 cut(s)	2287, 2435, 2764, 3507
TauI	GCSGC	1 cut(s)	119
TseFI	GTSAC	8 cut(s)	916, 1229, 2347, 3337, 3493, 4329, 4813, 5478
TseI	GCWGC	9 cut(s)	142, 571, 842, 1742, 1794, 2004, 2126, 2560, 4955
Tsp45I	GTSAC	8 cut(s)	916, 1229, 2347, 3337, 3493, 4329, 4813, 5478
TspGWI	ACGGA	4 cut(s)	643, 1637, 3260, 3764
Tth111I	GACNNNGTC	1 cut(s)	155
Van91I	CCANNNNNTGG	4 cut(s)	2122, 3839, 3929, 4735
VpaK11BI	GGWCC	9 cut(s)	123, 442, 703, 1867, 2638, 3196, 3268, 3863, 4726
VspI	ATTAAT	2 cut(s)	1754, 2232
XagI	CCTNNNNNAGG	1 cut(s)	27
XceI	RCATGY	4 cut(s)	1211, 3494, 3769, 4388
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1811, 3181
XhoI	CTCGAG	1 cut(s)	1122
XmaJI	CCTAGG	1 cut(s)	4988
XmiI	GTMKAC	1 cut(s)	4830
XmnI	GAANNNNTTC	2 cut(s)	1341, 5228
ZraI	GACGTC	1 cut(s)	157
ZrmI	AGTACT	1 cut(s)	2437
Zsp2I	ATGCAT	3 cut(s)	2907, 2995, 5635

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.