MD12G1052000.v1.1

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007318
Physical Location & Seq
Forward (+)
5893445 .. 5899167
5723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1052000.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGGGCCGGCTCGCACCGCTGTCGGAAGAGCCGATCATCAACGGAGAAGGCGCCGCGAACCGGTCGAGCAAAGGCATTCTGCGGAGCACTCAGCCGTGGCGGAACTGGATCAAGACCCACCTTTCTCCCCTCGTGCTCTTCCACAAGAGGTCCGACTTCAAAGTGCTTCTCGGTGTTCTGGGTTGCCCTCTCTTCCCTGTCTCTGCTCTCCCCAAACTACCCCTCAATGAGGTGTCTTCTACTGCACAATACATAATACAACACTTCACAGCTGCCACTGGTTGTCGCAAGCTAGAAGGCAAAGTGAAGAACATATTCGCAACCGGGAAAGTGATCATGGCAATGGTGAATGATCCCGGCCCCAGTGGCAGCTCAGCTGCCGGAGCCACCACAGTTTCGGAAAAAGGATGCTTTGTGATGTGGCAGATGGTTCCTAATAAGTGGCTCATTGAGCTAGTTCTAGGTGGTCACAAGGTGGCAGCTGGTAGCGACGGCATTGTCGCTTGGCGCCACACGCCCTGGCTAGGCGCCCACGCTGCCAAAGGTGGTGTTCGTCCCCTCCGGCGAGCTCTTCAGGGATTAGATCCAATGGCAATATCAGCTGTGTTTGCCCCAGCACAGTACATGGGAGAAAAGCAAATCACAGGCGTCGATTGCTTCGTGTTGAAGTTGTCAGCCGATCAAACGGATCTGGCTGAACGAAGCGATAGCACAGCAGAGATGATCAAGCATGTAATATTTGGCTACTTCAGCCAAAGAAATGGGCTGCTGGTGTACTTAGAGGACTCATACTTAACCAGGATCCACTCACCTGGCGCCTACCCTACCTACTGGGAGACCACAATGTCGACGAAAATCGAGGACTATCGGACGGTGGAGGGTGTGATGATCGCGCACGCCGGTCAGACGAGCGTGATCATCACACGGTTTGGGGACAACCTAAAGACGGGATCCGCGATCACGCGGCTGGAAGAAACATGGACCATTGATGATGTTGCATTCAACGTCCCCGGCTTGTCCATGGATTGCTTCATTCCTCCTAAAGAAGTACAGAAAGATTACCCCAAGAAAGATTTTGATTGGAGATCACCGTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

367

Amino Acids

40.19

Weight (kDa)

8.68

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 105 - 347 2.1e-105 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 497, 844
AccB1I GGYRCC 4 cut(s) 50, 507, 527, 815
AccI GTMKAC 1 cut(s) 848
AccII CGCG 4 cut(s) 56, 893, 956, 964
AciI CCGC 6 cut(s) 17, 54, 82, 100, 954, 964
AclWI GGATC 8 cut(s) 116, 347, 578, 696, 796, 809, 945, 958
AcuI CTGAAG 2 cut(s) 557, 733
AcyI GRCGYC 5 cut(s) 51, 508, 528, 648, 816
AdeI CACNNNGTG 1 cut(s) 475
AfaI GTAC 3 cut(s) 623, 776, 1050
AfiI CCNNNNNNNGG 4 cut(s) 60, 229, 524, 946
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 160, 667, 1003
AjnI CCWGG 3 cut(s) 518, 797, 811
AluBI AGCT 8 cut(s) 272, 292, 372, 377, 454, 482, 569, 602
AluI AGCT 8 cut(s) 272, 292, 372, 377, 454, 482, 569, 602
Alw21I GWGCWC 3 cut(s) 89, 138, 571
Alw26I GTCTC 2 cut(s) 205, 830
AlwI GGATC 8 cut(s) 116, 347, 578, 696, 796, 809, 945, 958
AoxI GGCC 2 cut(s) 4, 358
ApeKI GCWGC 6 cut(s) 272, 369, 377, 479, 536, 766
AsiGI ACCGGT 1 cut(s) 60
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 5 cut(s) 53, 510, 530, 818, 895
AspS9I GGNCC 4 cut(s) 4, 150, 359, 981
AsuC2I CCSGG 3 cut(s) 325, 357, 1011
AsuHPI GGTGA 3 cut(s) 358, 801, 1080
AvaII GGWCC 2 cut(s) 150, 981
BamHI GGATCC 2 cut(s) 801, 950
BanI GGYRCC 4 cut(s) 50, 507, 527, 815
BanII GRGCYC 1 cut(s) 571
BauI CACGAG 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 89, 138, 571
BbvI GCAGC 6 cut(s) 259, 364, 381, 491, 523, 753
BccI CCATC 1 cut(s) 421
BceAI ACGGC 2 cut(s) 79, 508
BcgI CGANNNNNNTGC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 520, 799, 813
BclI TGATCA 3 cut(s) 333, 723, 915
BcnI CCSGG 3 cut(s) 325, 357, 1011
BcoDI GTCTC 2 cut(s) 205, 830
BfaI CTAG 4 cut(s) 293, 455, 461, 524
BfoI RGCGCY 4 cut(s) 54, 511, 531, 819
BglI GCCNNNNNGGC 1 cut(s) 366
BisI GCNGC 8 cut(s) 54, 273, 370, 378, 480, 537, 767, 965
BlpI GCTNAGC 1 cut(s) 373
BlsI GCNGC 8 cut(s) 55, 274, 371, 379, 481, 538, 768, 966
Bme1390I CCNGG 6 cut(s) 325, 357, 520, 799, 813, 1011
Bme18I GGWCC 2 cut(s) 150, 981
BmgT120I GGNCC 4 cut(s) 4, 150, 359, 981
BmiI GGNNCC 9 cut(s) 52, 361, 385, 432, 509, 529, 803, 817, 952
BmrFI CCNGG 6 cut(s) 325, 357, 520, 799, 813, 1011
BmrI ACTGGG 2 cut(s) 357, 841
BmsI GCATC 1 cut(s) 398
BmuI ACTGGG 2 cut(s) 357, 841
BpiI GAAGAC 1 cut(s) 228
Bpu1102I GCTNAGC 1 cut(s) 373
BpuMI CCSGG 3 cut(s) 325, 357, 1011
BsaHI GRCGYC 5 cut(s) 51, 508, 528, 648, 816
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 4 cut(s) 95, 518, 1009, 1020
BsaWI WCCGGW 1 cut(s) 60
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 229, 524, 946
Bse118I RCCGGY 3 cut(s) 6, 60, 899
Bse1I ACTGG 4 cut(s) 110, 283, 363, 836
Bse3DI GCAATG 1 cut(s) 348
BseBI CCWGG 3 cut(s) 520, 799, 813
BseDI CCNNGG 4 cut(s) 95, 518, 1009, 1020
BseGI GGATG 1 cut(s) 413
BseLI CCNNNNNNNGG 4 cut(s) 60, 229, 524, 946
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 104, 387
BseNI ACTGG 4 cut(s) 110, 283, 363, 836
BseXI GCAGC 6 cut(s) 259, 364, 381, 491, 523, 753
BseYI CCCAGC 1 cut(s) 613
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 4 cut(s) 56, 893, 956, 964
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 2 cut(s) 6, 360
BshNI GGYRCC 4 cut(s) 50, 507, 527, 815
BshTI ACCGGT 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 3 cut(s) 89, 138, 571
BsiSI CCGG 8 cut(s) 7, 61, 324, 357, 381, 562, 900, 1011
BslFI GGGAC 3 cut(s) 540, 947, 992
BslI CCNNNNNNNGG 4 cut(s) 60, 229, 524, 946
BsmAI GTCTC 2 cut(s) 205, 830
BsmFI GGGAC 3 cut(s) 540, 947, 992
BsmI GAATGC 2 cut(s) 75, 998
BsnI GGCC 2 cut(s) 6, 360
Bso31I GGTCTC 1 cut(s) 830
Bsp1286I GDGCHC 3 cut(s) 89, 138, 571
Bsp1720I GCTNAGC 1 cut(s) 373
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 6 cut(s) 17, 54, 82, 100, 954, 964
BspANI GGCC 2 cut(s) 6, 360
BspCNI CTCAG 2 cut(s) 103, 386
BspFNI CGCG 4 cut(s) 56, 893, 956, 964
BspLI GGNNCC 9 cut(s) 52, 361, 385, 432, 509, 529, 803, 817, 952
BspPI GGATC 8 cut(s) 116, 347, 578, 696, 796, 809, 945, 958
BspQI GCTCTTC 3 cut(s) 21, 143, 576
BspT107I GGYRCC 4 cut(s) 50, 507, 527, 815
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 348
BsrFI RCCGGY 3 cut(s) 6, 60, 899
BsrI ACTGG 4 cut(s) 110, 283, 363, 836
BssAI RCCGGY 3 cut(s) 6, 60, 899
BssECI CCNNGG 4 cut(s) 95, 518, 1009, 1020
BssNI GRCGYC 5 cut(s) 51, 508, 528, 648, 816
BssSI CACGAG 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 1020
Bst2BI CACGAG 1 cut(s) 131
Bst2UI CCWGG 3 cut(s) 520, 799, 813
Bst4CI ACNGT 5 cut(s) 394, 621, 874, 927, 1092
Bst6I CTCTTC 4 cut(s) 21, 143, 197, 576
BstACI GRCGYC 5 cut(s) 51, 508, 528, 648, 816
BstC8I GCNNGC 5 cut(s) 8, 12, 290, 567, 897
BstDEI CTNAG 3 cut(s) 90, 373, 778
BstDSI CCRYGG 2 cut(s) 95, 1020
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 413
BstFNI CGCG 4 cut(s) 56, 893, 956, 964
BstH2I RGCGCY 4 cut(s) 54, 511, 531, 819
BstHHI GCGC 5 cut(s) 53, 510, 530, 818, 895
BstMAI GTCTC 2 cut(s) 205, 830
BstMCI CGRYCG 1 cut(s) 65
BstNI CCWGG 3 cut(s) 520, 799, 813
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 6 cut(s) 323, 355, 518, 797, 811, 1009
BstUI CGCG 4 cut(s) 56, 893, 956, 964
BstV1I GCAGC 6 cut(s) 259, 364, 381, 491, 523, 753
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 4 cut(s) 583, 688, 801, 950
BstXI CCANNNNNNTGG 2 cut(s) 761, 812
BstYI RGATCY 4 cut(s) 583, 688, 801, 950
BsuRI GGCC 2 cut(s) 6, 360
BtgI CCRYGG 2 cut(s) 95, 1020
BtsCI GGATG 1 cut(s) 413
BtsIMutI CAGTG 2 cut(s) 276, 370
Cac8I GCNNGC 5 cut(s) 8, 12, 290, 567, 897
CfoI GCGC 5 cut(s) 53, 510, 530, 818, 895
Cfr10I RCCGGY 3 cut(s) 6, 60, 899
Cfr13I GGNCC 4 cut(s) 4, 150, 359, 981
CseI GACGC 1 cut(s) 637
Csp6I GTAC 3 cut(s) 622, 775, 1049
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 5 cut(s) 337, 625, 731, 978, 1021
CviQI GTAC 3 cut(s) 622, 775, 1049
DdeI CTNAG 3 cut(s) 90, 373, 778
DinI GGCGCC 4 cut(s) 52, 509, 529, 817
DraIII CACNNNGTG 1 cut(s) 475
DrdI GACNNNNNNGTC 2 cut(s) 497, 844
DseDI GACNNNNNNGTC 2 cut(s) 497, 844
Eam1104I CTCTTC 4 cut(s) 21, 143, 197, 576
EarI CTCTTC 4 cut(s) 21, 143, 197, 576
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 1020
Eco24I GRGCYC 1 cut(s) 571
Eco31I GGTCTC 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 150, 981
Eco53kI GAGCTC 1 cut(s) 569
Eco57I CTGAAG 2 cut(s) 557, 733
EcoICRI GAGCTC 1 cut(s) 569
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRII CCWGG 3 cut(s) 518, 797, 811
EcoT14I CCWWGG 1 cut(s) 1020
EcoT38I GRGCYC 1 cut(s) 571
EgeI GGCGCC 4 cut(s) 52, 509, 529, 817
EheI GGCGCC 4 cut(s) 52, 509, 529, 817
ErhI CCWWGG 1 cut(s) 1020
FaeI CATG 5 cut(s) 340, 628, 734, 981, 1024
FaiI YATR 8 cut(s) 254, 314, 338, 626, 732, 790, 979, 1022
FaqI GGGAC 3 cut(s) 540, 947, 992
FatI CATG 5 cut(s) 336, 624, 730, 977, 1020
FbaI TGATCA 3 cut(s) 333, 723, 915
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 8 cut(s) 54, 273, 370, 378, 480, 537, 767, 965
FokI GGATG 1 cut(s) 420
FriOI GRGCYC 1 cut(s) 571
Fsp4HI GCNGC 8 cut(s) 54, 273, 370, 378, 480, 537, 767, 965
FspBI CTAG 4 cut(s) 293, 455, 461, 524
GlaI GCGC 5 cut(s) 52, 509, 529, 817, 894
GluI GCNGC 8 cut(s) 54, 273, 370, 378, 480, 537, 767, 965
GsaI CCCAGC 1 cut(s) 617
HaeII RGCGCY 4 cut(s) 54, 511, 531, 819
HaeIII GGCC 2 cut(s) 6, 360
HapII CCGG 8 cut(s) 7, 61, 324, 357, 381, 562, 900, 1011
HgaI GACGC 1 cut(s) 637
HhaI GCGC 5 cut(s) 53, 510, 530, 818, 895
Hin1I GRCGYC 5 cut(s) 51, 508, 528, 648, 816
Hin1II CATG 5 cut(s) 340, 628, 734, 981, 1024
Hin6I GCGC 5 cut(s) 51, 508, 528, 816, 893
HinP1I GCGC 5 cut(s) 51, 508, 528, 816, 893
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HinfI GANTC 1 cut(s) 785
HpaII CCGG 8 cut(s) 7, 61, 324, 357, 381, 562, 900, 1011
HphI GGTGA 3 cut(s) 358, 801, 1080
Hpy166II GTNNAC 2 cut(s) 775, 849
Hpy188I TCNGA 5 cut(s) 25, 154, 400, 870, 906
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 2 cut(s) 775, 849
Hpy99I CGWCG 3 cut(s) 494, 653, 853
HpyAV CCTTC 2 cut(s) 41, 290
HpyCH4III ACNGT 5 cut(s) 394, 621, 874, 927, 1092
HpyCH4IV ACGT 1 cut(s) 1005
HpyCH4V TGCA 2 cut(s) 245, 998
HpyF3I CTNAG 3 cut(s) 90, 373, 778
HpySE526I ACGT 1 cut(s) 1005
Hsp92I GRCGYC 5 cut(s) 51, 508, 528, 648, 816
Hsp92II CATG 5 cut(s) 340, 628, 734, 981, 1024
HspAI GCGC 5 cut(s) 51, 508, 528, 816, 893
KasI GGCGCC 4 cut(s) 50, 507, 527, 815
KroI GCCGGC 1 cut(s) 6
KroNI GCCGGC 1 cut(s) 8
Ksp22I TGATCA 3 cut(s) 333, 723, 915
LguI GCTCTTC 3 cut(s) 21, 143, 576
LmnI GCTCC 2 cut(s) 84, 383
Lsp1109I GCAGC 6 cut(s) 259, 364, 381, 491, 523, 753
LweI GCATC 1 cut(s) 398
MaeI CTAG 4 cut(s) 293, 455, 461, 524
MaeII ACGT 1 cut(s) 1005
MaeIII GTNAC 1 cut(s) 467
MboII GAAGA 7 cut(s) 38, 130, 184, 228, 319, 563, 983
MflI RGATCY 4 cut(s) 583, 688, 801, 950
MhlI GDGCHC 3 cut(s) 89, 138, 571
Mly113I GGCGCC 4 cut(s) 51, 508, 528, 816
MlyI GAGTC 1 cut(s) 779
MmeI TCCRAC 2 cut(s) 177, 1074
MroNI GCCGGC 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 1 cut(s) 794
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 5 cut(s) 19, 272, 377, 482, 602
MspI CCGG 8 cut(s) 7, 61, 324, 357, 381, 562, 900, 1011
MspR9I CCNGG 6 cut(s) 325, 357, 520, 799, 813, 1011
Mva1269I GAATGC 2 cut(s) 75, 998
MvaI CCWGG 3 cut(s) 520, 799, 813
MvnI CGCG 4 cut(s) 56, 893, 956, 964
NaeI GCCGGC 1 cut(s) 8
NarI GGCGCC 4 cut(s) 51, 508, 528, 816
NciI CCSGG 3 cut(s) 325, 357, 1011
NcoI CCATGG 1 cut(s) 1020
NgoMIV GCCGGC 1 cut(s) 6
NlaIII CATG 5 cut(s) 340, 628, 734, 981, 1024
NlaIV GGNNCC 9 cut(s) 52, 361, 385, 432, 509, 529, 803, 817, 952
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 734
PciSI GCTCTTC 3 cut(s) 21, 143, 576
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 657, 953
PctI GAATGC 2 cut(s) 75, 998
PdiI GCCGGC 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 8 cut(s) 55, 274, 371, 379, 481, 538, 768, 966
PleI GAGTC 1 cut(s) 779
PluTI GGCGCC 4 cut(s) 54, 511, 531, 819
PpsI GAGTC 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 571
Psp6I CCWGG 3 cut(s) 518, 797, 811
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 3 cut(s) 518, 797, 811
PspN4I GGNNCC 9 cut(s) 52, 361, 385, 432, 509, 529, 803, 817, 952
PspPI GGNCC 4 cut(s) 4, 150, 359, 981
PsuI RGATCY 4 cut(s) 583, 688, 801, 950
PvuII CAGCTG 4 cut(s) 272, 377, 482, 602
RsaI GTAC 3 cut(s) 623, 776, 1050
RsaNI GTAC 3 cut(s) 622, 775, 1049
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 571
SalI GTCGAC 1 cut(s) 847
SapI GCTCTTC 3 cut(s) 21, 143, 576
SaqAI TTAA 1 cut(s) 794
SatI GCNGC 8 cut(s) 54, 273, 370, 378, 480, 537, 767, 965
Sau96I GGNCC 4 cut(s) 4, 150, 359, 981
SchI GAGTC 1 cut(s) 779
ScrFI CCNGG 6 cut(s) 325, 357, 520, 799, 813, 1011
SduI GDGCHC 3 cut(s) 89, 138, 571
SfaNI GCATC 1 cut(s) 398
SfoI GGCGCC 4 cut(s) 52, 509, 529, 817
SinI GGWCC 2 cut(s) 150, 981
SmiMI CAYNNNNRTG 1 cut(s) 341
SsiI CCGC 6 cut(s) 17, 54, 82, 100, 954, 964
SspDI GGCGCC 4 cut(s) 50, 507, 527, 815
SspI AATATT 1 cut(s) 738
SspMI CTAG 4 cut(s) 293, 455, 461, 524
SstI GAGCTC 1 cut(s) 571
StyD4I CCNGG 6 cut(s) 323, 355, 518, 797, 811, 1009
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 5 cut(s) 394, 621, 874, 927, 1092
TaiI ACGT 1 cut(s) 1008
TaqI TCGA 4 cut(s) 65, 651, 848, 858
TatI WGTACW 3 cut(s) 621, 774, 1048
TauI GCSGC 2 cut(s) 56, 967
Tru1I TTAA 1 cut(s) 794
Tru9I TTAA 1 cut(s) 794
TscAI CASTG 2 cut(s) 283, 370
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 6 cut(s) 272, 369, 377, 479, 536, 766
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 1 cut(s) 1021
TspGWI ACGGA 2 cut(s) 57, 701
TspRI CASTG 2 cut(s) 283, 370
VpaK11BI GGWCC 2 cut(s) 150, 981
XagI CCTNNNNNAGG 1 cut(s) 227
XceI RCATGY 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 4 cut(s) 293, 455, 461, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.