Rh3BG251400

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0007318
Physical Location & Seq
Forward (+)
24260024 .. 24263746
3723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG251400.1

Sequence Viewer

Length: 1098 bp
ATGGGTCGTCTTGCACCATTATCAGAAGAACCCAATATTAATGAAGAAGAAGATGCCACAAATCGCTCAAGCAAAGGCATAATGAGGAGTCAGACATGGAGGAAATGGATCAAGACCCACCTCACCTCCCTTGCCATCTTCAACAAGAGGTCTGACTTCAAGGTCCTCCTCAGCGTTCTGGGTTGCCCTCTCTTCCCTGTTTCTGCCCTCCCCGAACTGCCCCTCAATGAGGTCTCCTCCACTGCACAATACATAATACAACACTTCACAGCAGCCACAGGCTGCCGCAAGCTAGAAGGTAGAGTCAAGAACATTTTTGCAACCGGAAAAGTGAACATGGCGATGGTGGATGATCCCGGTCCTGGCAGCTCCATGAGTGGAGCCACCACCATATCAGAAAAAGGGTGCTTTGTAATGTGGCAGATGATTCCCAATAAGTGGCTGATTGAGCTAGTTCTGGGTGGTCACAAGGTAGCAGCTGGTAGTGATGGCAATGTGGCATGGCGCCACACGCCGTGGCTTGCTGCGCATGCTGCCAAGGGCGGTGTTCGTCCTCTCCGGCGAGCACTCCAGGGACTAGACCCCATGGCGATATCAGCTGTATTTGCCCCAGCACAGTACATGGGCGAAAAGCAAATCTCGGACGTCGACTGCTTTGTCTTAAAATTGTCAGCCGATCAGACAGATCTGTCTGAAAGAAGCGATAGTACTGCAGAGATGATCAAACATGTGATATTCGGTTACTTCAGCCAAAGAAATGGACTACTCGTGCACTTAGAAGACTCTTACTTAACTAGGATTCAATCACCTGGTGCCTATCCTACATACTGGGAGACCACAATGTCGACTAAGATCGAGGATTACCGGACGGTGGAAGAGGTGATGATCGCCCATGCCGGTCAGACGAGTGTGGTCATCACACGGTTCGGGGACAATTTAAAGACTGGCTCCGCAATCACACGGTTAGAAGAAACATGGACCATCGATGATCTTGCTTTCAATGTTCAGGGCCTCTCAATGGATTGCTTCATTCCTCCTAAAGAAGTGCAGAAAGATCACCCGAAGAAAACTCTAGATTGGAGATCACCATTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

365

Amino Acids

40.54

Weight (kDa)

6.8

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 105 - 346 2.8e-105 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 161, 656, 688, 841
AatII GACGTC 1 cut(s) 648
Acc16I TGCGCA 1 cut(s) 528
AccB1I GGYRCC 2 cut(s) 504, 812
AccB7I CCANNNNNTGG 1 cut(s) 438
AccI GTMKAC 2 cut(s) 648, 845
AciI CCGC 3 cut(s) 286, 543, 951
AclWI GGATC 2 cut(s) 116, 347
AcuI CTGAAG 1 cut(s) 730
AcyI GRCGYC 2 cut(s) 505, 645
AdeI CACNNNGTG 2 cut(s) 516, 812
AfaI GTAC 2 cut(s) 620, 709
AfiI CCNNNNNNNGG 5 cut(s) 229, 362, 438, 871, 1018
AflIII ACRYGT 1 cut(s) 727
AgsI TTSAA 4 cut(s) 142, 160, 803, 1000
AjnI CCWGG 3 cut(s) 361, 570, 808
AluBI AGCT 5 cut(s) 292, 369, 451, 479, 599
AluI AGCT 5 cut(s) 292, 369, 451, 479, 599
Alw21I GWGCWC 2 cut(s) 568, 774
Alw26I GTCTC 2 cut(s) 238, 827
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 2 cut(s) 116, 347
AoxI GGCC 1 cut(s) 1009
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 6 cut(s) 272, 282, 366, 476, 524, 533
AseI ATTAAT 1 cut(s) 39
AspLEI GCGC 2 cut(s) 507, 529
AspS9I GGNCC 4 cut(s) 163, 359, 978, 1009
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 5 cut(s) 115, 798, 892, 1049, 1077
AvaII GGWCC 3 cut(s) 163, 359, 978
BaeGI GKGCMC 1 cut(s) 774
BanI GGYRCC 2 cut(s) 504, 812
BarI GAAGNNNNNNTAC 2 cut(s) 691, 723
BauI CACGAG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 2 cut(s) 568, 774
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 6 cut(s) 269, 284, 378, 488, 511, 520
BccI CCATC 4 cut(s) 143, 337, 482, 989
BceAI ACGGC 1 cut(s) 499
BcgI CGANNNNNNTGC 4 cut(s) 692, 726, 974, 1008
BciT130I CCWGG 3 cut(s) 363, 572, 810
BclI TGATCA 1 cut(s) 720
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 238, 827
BfaI CTAG 5 cut(s) 293, 452, 578, 795, 1073
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 1 cut(s) 508
BglII AGATCT 1 cut(s) 685
BisI GCNGC 7 cut(s) 273, 283, 286, 367, 477, 525, 534
BlsI GCNGC 7 cut(s) 274, 284, 287, 368, 478, 526, 535
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 4 cut(s) 357, 363, 572, 810
Bme18I GGWCC 3 cut(s) 163, 359, 978
BmgT120I GGNCC 4 cut(s) 163, 359, 978, 1009
BmiI GGNNCC 4 cut(s) 382, 506, 814, 949
BmrFI CCNGG 4 cut(s) 357, 363, 572, 810
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 786
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 554
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 52
BpuMI CCSGG 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 984
BsaHI GRCGYC 2 cut(s) 505, 645
BsaI GGTCTC 2 cut(s) 238, 827
BsaJI CCNNGG 4 cut(s) 515, 537, 571, 585
BsaWI WCCGGW 2 cut(s) 323, 864
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 362, 438, 871, 1018
Bse118I RCCGGY 1 cut(s) 896
Bse1I ACTGG 2 cut(s) 833, 949
Bse3DI GCAATG 2 cut(s) 499, 1088
BseBI CCWGG 3 cut(s) 363, 572, 810
BseCI ATCGAT 1 cut(s) 984
BseDI CCNNGG 4 cut(s) 515, 537, 571, 585
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 5 cut(s) 229, 362, 438, 871, 1018
BseMI GCAATG 2 cut(s) 499, 1088
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 2 cut(s) 833, 949
BseRI GAGGAG 3 cut(s) 100, 158, 226
BseSI GKGCMC 1 cut(s) 774
BseXI GCAGC 6 cut(s) 269, 284, 378, 488, 511, 520
BseYI CCCAGC 1 cut(s) 610
BsgI GTGCAG 2 cut(s) 228, 1067
BshFI GGCC 1 cut(s) 1011
BshNI GGYRCC 2 cut(s) 504, 812
BshVI ATCGAT 1 cut(s) 984
BsiHKAI GWGCWC 2 cut(s) 568, 774
BsiSI CCGG 5 cut(s) 324, 357, 559, 865, 897
BslFI GGGAC 2 cut(s) 588, 944
BslI CCNNNNNNNGG 5 cut(s) 229, 362, 438, 871, 1018
BsmAI GTCTC 2 cut(s) 238, 827
BsmFI GGGAC 2 cut(s) 588, 944
BsnI GGCC 1 cut(s) 1011
Bso31I GGTCTC 2 cut(s) 238, 827
Bsp1286I GDGCHC 2 cut(s) 568, 774
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 3 cut(s) 286, 543, 951
BspANI GGCC 1 cut(s) 1011
BspCNI CTCAG 1 cut(s) 183
BspDI ATCGAT 1 cut(s) 984
BspLI GGNNCC 4 cut(s) 382, 506, 814, 949
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 2 cut(s) 116, 347
BspT107I GGYRCC 2 cut(s) 504, 812
BspTNI GGTCTC 2 cut(s) 238, 827
BsrDI GCAATG 2 cut(s) 499, 1088
BsrFI RCCGGY 1 cut(s) 896
BsrI ACTGG 2 cut(s) 833, 949
BssAI RCCGGY 1 cut(s) 896
BssECI CCNNGG 4 cut(s) 515, 537, 571, 585
BssNI GRCGYC 2 cut(s) 505, 645
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 537, 585
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 3 cut(s) 363, 572, 810
Bst4CI ACNGT 4 cut(s) 618, 871, 924, 963
Bst6I CTCTTC 2 cut(s) 197, 870
BstACI GRCGYC 2 cut(s) 505, 645
BstC8I GCNNGC 4 cut(s) 290, 522, 531, 564
BstDEI CTNAG 3 cut(s) 170, 775, 849
BstDSI CCRYGG 2 cut(s) 515, 585
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 355
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 2 cut(s) 507, 529
BstMAI GTCTC 2 cut(s) 238, 827
BstMWI GCNNNNNNNGC 7 cut(s) 448, 511, 526, 530, 533, 596, 605
BstNI CCWGG 3 cut(s) 363, 572, 810
BstNSI RCATGY 2 cut(s) 533, 731
BstSCI CCNGG 4 cut(s) 355, 361, 570, 808
BstSFI CTRYAG 1 cut(s) 711
BstSLI GKGCMC 1 cut(s) 774
BstV1I GCAGC 6 cut(s) 269, 284, 378, 488, 511, 520
BstV2I GAAGAC 1 cut(s) 786
BstX2I RGATCY 1 cut(s) 685
BstXI CCANNNNNNTGG 1 cut(s) 758
BstYI RGATCY 1 cut(s) 685
Bsu15I ATCGAT 1 cut(s) 984
BsuRI GGCC 1 cut(s) 1011
BsuTUI ATCGAT 1 cut(s) 984
BtgI CCRYGG 2 cut(s) 515, 585
BtgZI GCGATG 1 cut(s) 356
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 4 cut(s) 290, 522, 531, 564
CfoI GCGC 2 cut(s) 507, 529
Cfr10I RCCGGY 1 cut(s) 896
Cfr13I GGNCC 4 cut(s) 163, 359, 978, 1009
ClaI ATCGAT 1 cut(s) 984
CsiI ACCWGGT 1 cut(s) 808
Csp6I GTAC 2 cut(s) 619, 708
CviQI GTAC 2 cut(s) 619, 708
DdeI CTNAG 3 cut(s) 170, 775, 849
DinI GGCGCC 1 cut(s) 506
DraI TTTAAA 1 cut(s) 939
DraIII CACNNNGTG 2 cut(s) 516, 812
DrdI GACNNNNNNGTC 4 cut(s) 161, 656, 688, 841
DseDI GACNNNNNNGTC 4 cut(s) 161, 656, 688, 841
Eam1104I CTCTTC 2 cut(s) 197, 870
EarI CTCTTC 2 cut(s) 197, 870
Eco130I CCWWGG 2 cut(s) 537, 585
Eco31I GGTCTC 2 cut(s) 238, 827
Eco32I GATATC 1 cut(s) 594
Eco47I GGWCC 3 cut(s) 163, 359, 978
Eco57I CTGAAG 1 cut(s) 730
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoO109I RGGNCCY 2 cut(s) 163, 1009
EcoRII CCWGG 3 cut(s) 361, 570, 808
EcoRV GATATC 1 cut(s) 594
EcoT14I CCWWGG 2 cut(s) 537, 585
EgeI GGCGCC 1 cut(s) 506
EheI GGCGCC 1 cut(s) 506
ErhI CCWWGG 2 cut(s) 537, 585
FaqI GGGAC 2 cut(s) 588, 944
FbaI TGATCA 1 cut(s) 720
FblI GTMKAC 2 cut(s) 648, 845
Fnu4HI GCNGC 7 cut(s) 273, 283, 286, 367, 477, 525, 534
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 7 cut(s) 273, 283, 286, 367, 477, 525, 534
FspBI CTAG 5 cut(s) 293, 452, 578, 795, 1073
FspI TGCGCA 1 cut(s) 528
GlaI GCGC 2 cut(s) 506, 528
GluI GCNGC 7 cut(s) 273, 283, 286, 367, 477, 525, 534
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 554
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 1 cut(s) 1011
HapII CCGG 5 cut(s) 324, 357, 559, 865, 897
HhaI GCGC 2 cut(s) 507, 529
Hin1I GRCGYC 2 cut(s) 505, 645
Hin6I GCGC 2 cut(s) 505, 527
HinP1I GCGC 2 cut(s) 505, 527
HincII GTYRAC 2 cut(s) 649, 846
HindII GTYRAC 2 cut(s) 649, 846
HinfI GANTC 5 cut(s) 88, 303, 427, 782, 799
HpaII CCGG 5 cut(s) 324, 357, 559, 865, 897
HphI GGTGA 5 cut(s) 115, 798, 892, 1049, 1077
Hpy166II GTNNAC 4 cut(s) 334, 649, 772, 846
Hpy188I TCNGA 8 cut(s) 25, 93, 154, 397, 643, 681, 694, 903
Hpy188III TCNNGA 3 cut(s) 112, 307, 1073
Hpy8I GTNNAC 4 cut(s) 334, 649, 772, 846
Hpy99I CGWCG 1 cut(s) 650
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 4 cut(s) 618, 871, 924, 963
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 7 cut(s) 14, 245, 320, 713, 772, 1048, 1093
HpyF10VI GCNNNNNNNGC 7 cut(s) 448, 511, 526, 530, 533, 596, 605
HpyF3I CTNAG 3 cut(s) 170, 775, 849
HpySE526I ACGT 1 cut(s) 645
Hsp92I GRCGYC 2 cut(s) 505, 645
HspAI GCGC 2 cut(s) 505, 527
KasI GGCGCC 1 cut(s) 504
Ksp22I TGATCA 1 cut(s) 720
LmnI GCTCC 3 cut(s) 374, 380, 953
Lsp1109I GCAGC 6 cut(s) 269, 284, 378, 488, 511, 520
LweI GCATC 1 cut(s) 43
MabI ACCWGGT 1 cut(s) 808
MaeI CTAG 5 cut(s) 293, 452, 578, 795, 1073
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 2 cut(s) 464, 740
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 568, 774
MluCI AATT 2 cut(s) 665, 934
Mly113I GGCGCC 1 cut(s) 505
MlyI GAGTC 3 cut(s) 97, 312, 776
MseI TTAA 4 cut(s) 39, 662, 791, 938
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 479, 599
MspI CCGG 5 cut(s) 324, 357, 559, 865, 897
MspR9I CCNGG 4 cut(s) 357, 363, 572, 810
MvaI CCWGG 3 cut(s) 363, 572, 810
MwoI GCNNNNNNNGC 7 cut(s) 448, 511, 526, 530, 533, 596, 605
NarI GGCGCC 1 cut(s) 505
NciI CCSGG 1 cut(s) 357
NcoI CCATGG 1 cut(s) 585
NlaIV GGNNCC 4 cut(s) 382, 506, 814, 949
NmuCI GTSAC 1 cut(s) 464
NsbI TGCGCA 1 cut(s) 528
NspI RCATGY 2 cut(s) 533, 731
PaeI GCATGC 1 cut(s) 533
PciI ACATGT 1 cut(s) 727
PfeI GAWTC 2 cut(s) 427, 799
PflMI CCANNNNNTGG 1 cut(s) 438
PkrI GCNGC 7 cut(s) 274, 284, 287, 368, 478, 526, 535
PleI GAGTC 3 cut(s) 96, 311, 776
PluTI GGCGCC 1 cut(s) 508
PpsI GAGTC 3 cut(s) 96, 311, 776
PpuMI RGGWCCY 1 cut(s) 163
PscI ACATGT 1 cut(s) 727
PshBI ATTAAT 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 3 cut(s) 361, 570, 808
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 3 cut(s) 361, 570, 808
PspN4I GGNNCC 4 cut(s) 382, 506, 814, 949
PspPI GGNCC 4 cut(s) 163, 359, 978, 1009
PspPPI RGGWCCY 1 cut(s) 163
PstI CTGCAG 1 cut(s) 715
PsuI RGATCY 1 cut(s) 685
PvuII CAGCTG 2 cut(s) 479, 599
RsaI GTAC 2 cut(s) 620, 709
RsaNI GTAC 2 cut(s) 619, 708
RseI CAYNNNNRTG 1 cut(s) 341
SalI GTCGAC 2 cut(s) 647, 844
SaqAI TTAA 4 cut(s) 39, 662, 791, 938
SatI GCNGC 7 cut(s) 273, 283, 286, 367, 477, 525, 534
Sau96I GGNCC 4 cut(s) 163, 359, 978, 1009
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 3 cut(s) 97, 312, 776
ScrFI CCNGG 4 cut(s) 357, 363, 572, 810
SduI GDGCHC 2 cut(s) 568, 774
SexAI ACCWGGT 1 cut(s) 808
SfaNI GCATC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 711
SfoI GGCGCC 1 cut(s) 506
SinI GGWCC 3 cut(s) 163, 359, 978
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SphI GCATGC 1 cut(s) 533
Sse9I AATT 2 cut(s) 665, 934
SsiI CCGC 3 cut(s) 286, 543, 951
SspDI GGCGCC 1 cut(s) 504
SspI AATATT 1 cut(s) 37
SspMI CTAG 5 cut(s) 293, 452, 578, 795, 1073
StyD4I CCNGG 4 cut(s) 355, 361, 570, 808
StyI CCWWGG 2 cut(s) 537, 585
TaaI ACNGT 4 cut(s) 618, 871, 924, 963
TaiI ACGT 1 cut(s) 648
TaqI TCGA 4 cut(s) 648, 845, 855, 984
TasI AATT 2 cut(s) 665, 934
TatI WGTACW 2 cut(s) 618, 707
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 2 cut(s) 427, 799
Tru1I TTAA 4 cut(s) 39, 662, 791, 938
Tru9I TTAA 4 cut(s) 39, 662, 791, 938
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 6 cut(s) 272, 282, 366, 476, 524, 533
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 2 cut(s) 57, 1018
TspRI CASTG 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 438
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 3 cut(s) 163, 359, 978
VspI ATTAAT 1 cut(s) 39
XagI CCTNNNNNAGG 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 1072
XceI RCATGY 2 cut(s) 533, 731
XmiI GTMKAC 2 cut(s) 648, 845
XspI CTAG 5 cut(s) 293, 452, 578, 795, 1073
ZraI GACGTC 1 cut(s) 646
ZrmI AGTACT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.