Rorug03G0167500

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007318
Physical Location & Seq
Reverse (-)
13980315 .. 13982030
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0167500.1

Sequence Viewer

Length: 456 bp
ATGTGTCCACAACAGTTTCCCCAGACCAAGAACATGAAGAAAGCAGAAGACAGTAGCTTAATGAAGCTCAGGCTCATAATCGGCAAGCTACAAAAGGGTCTCTCACTGCTAACTGCAAGAGGCCCTCCTGATCATCGTTGCAGAGGCTGTGATGAAAAACTGGAGGTAGCAGCAATTGTACCAGAAGATGTCAAGGAAGGACATTTTGCAGTGTTTGCAGTTGTGGGTAAGGAGACAACGAGGTTTGTGACTAAGCTAGAGTCCTTGAATGACCCTGCACTTATGGAATTGCTAGAGCAAGCCAAGGACGAGTATGGGTTCCAACAGAAAGGTGCTCTTGAAGTTCATTGCAGGCCTGAGGAATTGCATTCAATTCTTGAAAACTGCAGGATGGATCAAAGTAGATTTGATGCTGCATCTAATGATGATTTTCTTACTGCCCTGGAAGGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

151

Amino Acids

17.0

Weight (kDa)

5.42

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 60 - 126 2.7e-13 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 402
AfaI GTAC 2 cut(s) 180, 452
AfiI CCNNNNNNNGG 1 cut(s) 447
AgsI TTSAA 4 cut(s) 268, 341, 372, 380
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 4 cut(s) 57, 67, 88, 256
AluI AGCT 4 cut(s) 57, 67, 88, 256
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 104, 227
AlwI GGATC 1 cut(s) 402
AlwNI CAGNNNCTG 1 cut(s) 147
AoxI GGCC 2 cut(s) 121, 353
ApeKI GCWGC 2 cut(s) 170, 413
AspS9I GGNCC 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 357
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 2 cut(s) 182, 400
BccI CCATC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 443
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 104, 227
BfaI CTAG 3 cut(s) 257, 293, 454
BfmI CTRYAG 1 cut(s) 385
BisI GCNGC 2 cut(s) 171, 414
BlsI GCNGC 2 cut(s) 172, 415
Bme1390I CCNGG 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 400, 425
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 303, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 165
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 443
BseDI CCNNGG 2 cut(s) 303, 441
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 82, 348
BseNI ACTGG 1 cut(s) 165
BseXI GCAGC 2 cut(s) 182, 400
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 2 cut(s) 123, 355
BsiHKAI GWGCWC 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 447
BsmAI GTCTC 2 cut(s) 104, 227
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 123, 355
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 2 cut(s) 130, 394
BspANI GGCC 2 cut(s) 123, 355
BspCNI CTCAG 2 cut(s) 81, 349
BspLI GGNNCC 1 cut(s) 320
BspMAI CTGCAG 1 cut(s) 389
BspPI GGATC 1 cut(s) 402
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 1 cut(s) 165
BssECI CCNNGG 2 cut(s) 303, 441
BssMI GATC 2 cut(s) 130, 394
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 2 cut(s) 15, 53
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 86, 300, 353
BstDEI CTNAG 3 cut(s) 68, 252, 357
BstF5I GGATG 1 cut(s) 396
BstKTI GATC 2 cut(s) 133, 397
BstMAI GTCTC 2 cut(s) 104, 227
BstMBI GATC 2 cut(s) 130, 394
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 441
BstSFI CTRYAG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 182, 400
BstV2I GAAGAC 1 cut(s) 54
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 2 cut(s) 123, 355
BtsCI GGATG 1 cut(s) 396
BtsI GCAGTG 2 cut(s) 104, 216
BtsIMutI CAGTG 2 cut(s) 104, 216
Cac8I GCNNGC 3 cut(s) 86, 300, 353
CaiI CAGNNNCTG 1 cut(s) 147
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 179, 451
CviAII CATG 1 cut(s) 34
CviJI RGCY 9 cut(s) 57, 67, 73, 88, 123, 147, 256, 302, 355
CviKI_1 RGCY 9 cut(s) 57, 67, 73, 88, 123, 147, 256, 302, 355
CviQI GTAC 2 cut(s) 179, 451
DdeI CTNAG 3 cut(s) 68, 252, 357
DpnI GATC 2 cut(s) 132, 396
DpnII GATC 2 cut(s) 130, 394
Eco130I CCWWGG 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 355
Eco31I GGTCTC 1 cut(s) 104
Eco81I CCTNAGG 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 37
FaiI YATR 4 cut(s) 35, 77, 284, 315
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FatI CATG 1 cut(s) 33
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 171, 414
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 171, 414
FspBI CTAG 3 cut(s) 257, 293, 454
GluI GCNGC 2 cut(s) 171, 414
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 2 cut(s) 123, 355
Hin1II CATG 1 cut(s) 37
HinfI GANTC 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 8
Hpy188III TCNNGA 3 cut(s) 128, 338, 377
Hpy8I GTNNAC 1 cut(s) 8
HpyAV CCTTC 2 cut(s) 191, 440
HpyCH4III ACNGT 2 cut(s) 15, 53
HpyCH4V TGCA 9 cut(s) 116, 141, 209, 218, 278, 351, 367, 387, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 3 cut(s) 68, 252, 357
Hsp92II CATG 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 2 cut(s) 130, 394
Lsp1109I GCAGC 2 cut(s) 182, 400
LweI GCATC 2 cut(s) 400, 425
MaeI CTAG 3 cut(s) 257, 293, 454
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 132, 396
MboI GATC 2 cut(s) 130, 394
MboII GAAGA 3 cut(s) 49, 59, 197
MfeI CAATTG 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 4 cut(s) 174, 287, 362, 372
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 6 cut(s) 113, 135, 137, 157, 234, 352
MseI TTAA 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 443
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 215
NdeII GATC 2 cut(s) 130, 394
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 247
PceI AGGCCT 1 cut(s) 355
PctI GAATGC 1 cut(s) 367
PkrI GCNGC 2 cut(s) 172, 415
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 320
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 147
RsaI GTAC 2 cut(s) 180, 452
RsaNI GTAC 2 cut(s) 179, 451
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 2 cut(s) 171, 414
Sau3AI GATC 2 cut(s) 130, 394
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 1 cut(s) 337
SetI ASST 7 cut(s) 59, 69, 90, 168, 245, 258, 334
SfaNI GCATC 2 cut(s) 400, 425
SfcI CTRYAG 1 cut(s) 385
Sse9I AATT 4 cut(s) 174, 287, 362, 372
SseBI AGGCCT 1 cut(s) 355
SspMI CTAG 3 cut(s) 257, 293, 454
StuI AGGCCT 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 441
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 15, 53
TasI AATT 4 cut(s) 174, 287, 362, 372
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 2 cut(s) 111, 216
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 2 cut(s) 170, 413
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 4 cut(s) 50, 77, 168, 335
TspRI CASTG 2 cut(s) 111, 216
XspI CTAG 3 cut(s) 257, 293, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.