MD12G1054800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007223
Physical Location & Seq
Reverse (-)
6190180 .. 6192964
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1054800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGTACTGCTCTAGATGCAATGGGTTACTGCTTGAAGGTTTTTTGCAGATTGTCGTGTATGGAAAGTCTTTAAAGCAGGAGGGAACAGGCGGGCAACTATCCTGCAACAGGTCAAGGGCCTCAAAAAATCAAAAAGATGGTGGATCAAGTATTACCAATGGGTGCCATGATGAAATCCCAGATCCATCTGTCCATCCTTGGGGTGGTCTAACCATAACGCGTGAGGGGTCTTTGACACTTATTGATTGCTATCTGTATTGTAAGTCTCTCAAGGGACTTCAAAATGTGTTTGACAGTGCACGCGCAAGGGAACGAGAACGTGAGTTGCTTTATCCTGATGCATGCGGTGGGGGAGGTCGGGGTTGGATTAGCCAAGGAATGGCAAGTTATGGCAGAGGACATGGAACAAGAGAAACATGTGCATTGCACACTGCCAGGCTTTCTTGCGATACATTGGTAGATTTCTGGTCAGCACTTGGAGAGGAAACTCGACAGTCTCTTCTTAGGATGAAGGAAGAGGATTTTATTGAGAGGCTCATGTACAGGTTTGATAGCAAGAGATTTTGCAGAGATTGCAGAAGGAATGTTATTCGTGAGTTCAAGGAGCTAAAGGAGCTGAAGCGCTTGCGGAGGGAACCTCGCTGCACTAATTGGTTTTGTGTTGCAGATACCGCATTTCAGTATGAGGTATCTGATGGCACAGTACAAGCTGATTGGCGCCATACTTTTGCCGACACTGTTGGGACATACCATCATTTTGAGTGGGCTGTTGGAACAGGTGAGGGAAAATCTGACATTCTGGAATTTGAAAATGTTGGCATGAATGGAAGTGTGAAGGCTAATGATCTAGAGCTCGGTGGTTTAAGTGCATGCTATATTACCCTGAGGGCTTGGAAACTAGATGGCCGTTGCACTGAGCTTTCTGTAAAAGCTCATGCATTGAAGGGTCAAGAATGTGTTCACTGTAGGCTTATAGTCGGAGACGGCTATGTAACAATCACAAGAGGAGAAAGTATTAGAAGATTTTTTGAACATGCTGAAGAGGCAGAGGAAGAAGAGGATGATGATTCCATGGACAAAGATGGAAATGAACTGGATGGAGAATGCTCCCGTCCGCAAAAACATGCGAAGAGTCCTGAACTTGCTCGAGAATTTCTTCTAGATGCTGCAACTGTTATTTTCAAGGAACAGGCATGTGGAAAAAGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.4

Weight (kDa)

5.77

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 162, 717
AccB7I CCANNNNNTGG 1 cut(s) 379
AccII CGCG 2 cut(s) 220, 303
AciI CCGC 5 cut(s) 90, 345, 628, 672, 1115
AclWI GGATC 2 cut(s) 151, 176
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 2 cut(s) 803, 1151
AcuI CTGAAG 2 cut(s) 638, 1059
AcyI GRCGYC 1 cut(s) 718
AfaI GTAC 3 cut(s) 5, 542, 705
AfeI AGCGCT 1 cut(s) 623
AfiI CCNNNNNNNGG 4 cut(s) 108, 199, 203, 379
AflIII ACRYGT 2 cut(s) 218, 416
AgsI TTSAA 7 cut(s) 35, 281, 601, 809, 943, 1031, 1183
AjnI CCWGG 1 cut(s) 434
AluBI AGCT 6 cut(s) 607, 616, 710, 853, 919, 932
AluI AGCT 6 cut(s) 607, 616, 710, 853, 919, 932
Alw21I GWGCWC 2 cut(s) 301, 855
Alw26I GTCTC 3 cut(s) 270, 501, 975
Alw44I GTGCAC 1 cut(s) 297
AlwI GGATC 2 cut(s) 151, 176
Ama87I CYCGRG 1 cut(s) 1146
Aor51HI AGCGCT 1 cut(s) 623
AoxI GGCC 2 cut(s) 117, 904
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 642, 1166
ApoI RAATTY 2 cut(s) 803, 1151
Asp700I GAANNNNTTC 2 cut(s) 957, 1155
AspLEI GCGC 3 cut(s) 305, 624, 720
AspS9I GGNCC 1 cut(s) 117
AsuHPI GGTGA 1 cut(s) 791
AvaI CYCGRG 1 cut(s) 1146
AxyI CCTNAGG 1 cut(s) 884
BaeGI GKGCMC 1 cut(s) 301
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BanI GGYRCC 2 cut(s) 162, 717
BanII GRGCYC 1 cut(s) 855
Bbv12I GWGCWC 2 cut(s) 301, 855
BbvI GCAGC 2 cut(s) 629, 1153
BccI CCATC 8 cut(s) 131, 193, 201, 689, 759, 896, 1076, 1091
BceAI ACGGC 2 cut(s) 891, 1000
BciT130I CCWGG 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 270, 501, 975
BfaI CTAG 4 cut(s) 12, 848, 899, 1160
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 2 cut(s) 625, 721
BisI GCNGC 2 cut(s) 643, 1167
BlsI GCNGC 2 cut(s) 644, 1168
Bme1390I CCNGG 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 1146
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 3 cut(s) 164, 636, 719
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 3 cut(s) 5, 328, 1153
BplI GAGNNNNNCTC 2 cut(s) 622, 654
BpuEI CTTGAG 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 718
BsaJI CCNNGG 3 cut(s) 197, 373, 1071
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 199, 203, 379
Bse1I ACTGG 1 cut(s) 1098
Bse21I CCTNAGG 1 cut(s) 884
Bse3DI GCAATG 2 cut(s) 25, 422
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 3 cut(s) 197, 373, 1071
BseGI GGATG 4 cut(s) 193, 513, 1066, 1102
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 4 cut(s) 108, 199, 203, 379
BseMI GCAATG 2 cut(s) 25, 422
BseMII CTCAG 2 cut(s) 875, 906
BseNI ACTGG 1 cut(s) 1098
BseRI GAGGAG 1 cut(s) 1020
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 2 cut(s) 629, 1153
BsgI GTGCAG 1 cut(s) 628
Bsh1236I CGCG 2 cut(s) 220, 303
BshFI GGCC 2 cut(s) 119, 906
BshNI GGYRCC 2 cut(s) 162, 717
BsiHKAI GWGCWC 2 cut(s) 301, 855
BsiHKCI CYCGRG 1 cut(s) 1146
BslFI GGGAC 2 cut(s) 288, 757
BslI CCNNNNNNNGG 4 cut(s) 108, 199, 203, 379
BsmAI GTCTC 3 cut(s) 270, 501, 975
BsmBI CGTCTC 1 cut(s) 975
BsmFI GGGAC 2 cut(s) 288, 757
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 2 cut(s) 119, 906
BsoBI CYCGRG 1 cut(s) 1146
Bsp1286I GDGCHC 2 cut(s) 301, 855
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 3 cut(s) 143, 181, 844
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 5 cut(s) 90, 345, 628, 672, 1115
BspANI GGCC 2 cut(s) 119, 906
BspCNI CTCAG 2 cut(s) 876, 907
BspFNI CGCG 2 cut(s) 220, 303
BspLI GGNNCC 3 cut(s) 164, 636, 719
BspPI GGATC 2 cut(s) 151, 176
BspT107I GGYRCC 2 cut(s) 162, 717
BsrDI GCAATG 2 cut(s) 25, 422
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 1098
BssECI CCNNGG 3 cut(s) 197, 373, 1071
BssMI GATC 3 cut(s) 143, 181, 844
BssNI GRCGYC 1 cut(s) 718
BssT1I CCWWGG 3 cut(s) 197, 373, 1071
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 6 cut(s) 296, 495, 703, 739, 965, 1174
Bst6I CTCTTC 5 cut(s) 504, 510, 1035, 1050, 1124
BstACI GRCGYC 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 573
BstAUI TGTACA 1 cut(s) 540
BstC8I GCNNGC 5 cut(s) 92, 301, 343, 626, 871
BstDEI CTNAG 3 cut(s) 503, 884, 915
BstDSI CCRYGG 1 cut(s) 1071
BstENI CCTNNNNNAGG 1 cut(s) 106
BstF5I GGATG 4 cut(s) 193, 513, 1066, 1102
BstFNI CGCG 2 cut(s) 220, 303
BstH2I RGCGCY 2 cut(s) 625, 721
BstHHI GCGC 3 cut(s) 305, 624, 720
BstKTI GATC 3 cut(s) 146, 184, 847
BstMAI GTCTC 3 cut(s) 270, 501, 975
BstMBI GATC 3 cut(s) 143, 181, 844
BstMWI GCNNNNNNNGC 5 cut(s) 15, 573, 613, 671, 1043
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 6 cut(s) 345, 420, 873, 1037, 1127, 1197
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 964
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 2 cut(s) 220, 303
BstV1I GCAGC 2 cut(s) 629, 1153
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
Bsu36I CCTNAGG 1 cut(s) 884
BsuRI GGCC 2 cut(s) 119, 906
BtgI CCRYGG 1 cut(s) 1071
BtsCI GGATG 4 cut(s) 193, 513, 1066, 1102
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 5 cut(s) 301, 429, 735, 912, 961
Cac8I GCNNGC 5 cut(s) 92, 301, 343, 626, 871
CfoI GCGC 3 cut(s) 305, 624, 720
Cfr13I GGNCC 1 cut(s) 117
Csp6I GTAC 3 cut(s) 4, 541, 704
CviQI GTAC 3 cut(s) 4, 541, 704
DdeI CTNAG 3 cut(s) 503, 884, 915
DinI GGCGCC 1 cut(s) 719
DpnI GATC 3 cut(s) 145, 183, 846
DpnII GATC 3 cut(s) 143, 181, 844
DraI TTTAAA 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 904
Eam1104I CTCTTC 5 cut(s) 504, 510, 1035, 1050, 1124
EarI CTCTTC 5 cut(s) 504, 510, 1035, 1050, 1124
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 3 cut(s) 197, 373, 1071
Eco24I GRGCYC 1 cut(s) 855
Eco47III AGCGCT 1 cut(s) 623
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 2 cut(s) 638, 1059
Eco81I CCTNAGG 1 cut(s) 884
Eco88I CYCGRG 1 cut(s) 1146
EcoICRI GAGCTC 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 3 cut(s) 197, 373, 1071
EcoT22I ATGCAT 2 cut(s) 343, 940
EcoT38I GRGCYC 1 cut(s) 855
EgeI GGCGCC 1 cut(s) 719
EheI GGCGCC 1 cut(s) 719
ErhI CCWWGG 3 cut(s) 197, 373, 1071
Esp3I CGTCTC 1 cut(s) 975
FaqI GGGAC 2 cut(s) 288, 757
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 2 cut(s) 643, 1167
FokI GGATG 4 cut(s) 180, 520, 1073, 1109
FriOI GRGCYC 1 cut(s) 855
Fsp4HI GCNGC 2 cut(s) 643, 1167
FspBI CTAG 4 cut(s) 12, 848, 899, 1160
GlaI GCGC 3 cut(s) 304, 623, 719
GluI GCNGC 2 cut(s) 643, 1167
HaeII RGCGCY 2 cut(s) 625, 721
HaeIII GGCC 2 cut(s) 119, 906
HhaI GCGC 3 cut(s) 305, 624, 720
Hin1I GRCGYC 1 cut(s) 718
Hin6I GCGC 3 cut(s) 303, 622, 718
HinP1I GCGC 3 cut(s) 303, 622, 718
HinfI GANTC 2 cut(s) 1067, 1132
HphI GGTGA 1 cut(s) 791
Hpy166II GTNNAC 2 cut(s) 299, 961
Hpy188I TCNGA 3 cut(s) 694, 793, 980
Hpy188III TCNNGA 9 cut(s) 12, 335, 593, 800, 848, 950, 1136, 1148, 1160
Hpy8I GTNNAC 2 cut(s) 299, 961
HpyAV CCTTC 5 cut(s) 29, 505, 573, 829, 937
HpyCH4III ACNGT 6 cut(s) 296, 495, 703, 739, 965, 1174
HpyCH4IV ACGT 1 cut(s) 319
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 573, 613, 671, 1043
HpyF3I CTNAG 3 cut(s) 503, 884, 915
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 718
HspAI GCGC 3 cut(s) 303, 622, 718
KasI GGCGCC 1 cut(s) 717
Kzo9I GATC 3 cut(s) 143, 181, 844
LmnI GCTCC 3 cut(s) 604, 613, 1112
Lsp1109I GCAGC 2 cut(s) 629, 1153
LweI GCATC 3 cut(s) 5, 328, 1153
MaeI CTAG 4 cut(s) 12, 848, 899, 1160
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 2 cut(s) 24, 991
MalI GATC 3 cut(s) 145, 183, 846
MboI GATC 3 cut(s) 143, 181, 844
MboII GAAGA 8 cut(s) 491, 527, 1032, 1052, 1064, 1067, 1141, 1148
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 2 cut(s) 301, 855
MluCI AATT 3 cut(s) 649, 803, 1151
MluI ACGCGT 1 cut(s) 218
Mly113I GGCGCC 1 cut(s) 718
MlyI GAGTC 1 cut(s) 1141
MmeI TCCRAC 3 cut(s) 344, 751, 958
Mph1103I ATGCAT 2 cut(s) 343, 940
MroXI GAANNNNTTC 2 cut(s) 957, 1155
MseI TTAA 2 cut(s) 71, 863
MspR9I CCNGG 1 cut(s) 436
Mva1269I GAATGC 1 cut(s) 1109
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 220, 303
MwoI GCNNNNNNNGC 5 cut(s) 15, 573, 613, 671, 1043
NarI GGCGCC 1 cut(s) 718
NcoI CCATGG 1 cut(s) 1071
NdeII GATC 3 cut(s) 143, 181, 844
NlaIV GGNNCC 3 cut(s) 164, 636, 719
NsiI ATGCAT 2 cut(s) 343, 940
NspI RCATGY 6 cut(s) 345, 420, 873, 1037, 1127, 1197
PaeI GCATGC 2 cut(s) 345, 873
PaeR7I CTCGAG 1 cut(s) 1146
PciI ACATGT 1 cut(s) 416
PctI GAATGC 1 cut(s) 1109
PdmI GAANNNNTTC 2 cut(s) 957, 1155
PfeI GAWTC 1 cut(s) 1067
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 2 cut(s) 644, 1168
PleI GAGTC 1 cut(s) 1140
PluTI GGCGCC 1 cut(s) 721
PpsI GAGTC 1 cut(s) 1140
PscI ACATGT 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 3 cut(s) 164, 636, 719
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 1 cut(s) 181
RsaI GTAC 3 cut(s) 5, 542, 705
RsaNI GTAC 3 cut(s) 4, 541, 704
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 2 cut(s) 71, 863
SatI GCNGC 2 cut(s) 643, 1167
Sau3AI GATC 3 cut(s) 143, 181, 844
Sau96I GGNCC 1 cut(s) 117
SchI GAGTC 1 cut(s) 1141
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 2 cut(s) 301, 855
SfaNI GCATC 3 cut(s) 5, 328, 1153
SfcI CTRYAG 1 cut(s) 964
SfoI GGCGCC 1 cut(s) 719
Sfr274I CTCGAG 1 cut(s) 1146
SlaI CTCGAG 1 cut(s) 1146
SmlI CTYRAG 2 cut(s) 269, 1146
SmoI CTYRAG 2 cut(s) 269, 1146
SphI GCATGC 2 cut(s) 345, 873
Sse9I AATT 3 cut(s) 649, 803, 1151
SsiI CCGC 5 cut(s) 90, 345, 628, 672, 1115
SspDI GGCGCC 1 cut(s) 717
SspMI CTAG 4 cut(s) 12, 848, 899, 1160
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 1 cut(s) 434
StyI CCWWGG 3 cut(s) 197, 373, 1071
TaaI ACNGT 6 cut(s) 296, 495, 703, 739, 965, 1174
TaiI ACGT 1 cut(s) 322
TaqI TCGA 2 cut(s) 490, 1147
TasI AATT 3 cut(s) 649, 803, 1151
TatI WGTACW 3 cut(s) 3, 540, 703
TfiI GAWTC 1 cut(s) 1067
Tru1I TTAA 2 cut(s) 71, 863
Tru9I TTAA 2 cut(s) 71, 863
TscAI CASTG 5 cut(s) 301, 436, 742, 919, 968
TseI GCWGC 2 cut(s) 642, 1166
TspDTI ATGAA 4 cut(s) 186, 524, 836, 1104
TspRI CASTG 5 cut(s) 301, 436, 742, 919, 968
Van91I CCANNNNNTGG 1 cut(s) 379
VneI GTGCAC 1 cut(s) 297
XagI CCTNNNNNAGG 1 cut(s) 106
XapI RAATTY 2 cut(s) 803, 1151
XbaI TCTAGA 3 cut(s) 11, 847, 1159
XceI RCATGY 6 cut(s) 345, 420, 873, 1037, 1127, 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 1146
XmnI GAANNNNTTC 2 cut(s) 957, 1155
XspI CTAG 4 cut(s) 12, 848, 899, 1160
Zsp2I ATGCAT 2 cut(s) 343, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.