Rorug03G0173000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007223
Physical Location & Seq
Forward (+)
14623334 .. 14625089
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0173000.1

Sequence Viewer

Length: 612 bp
ATGGCGGTTCCGGGCAGGGGTTTAGTGGAGGCTGACGACAACGATGACGAAAACGCTCTCTTTGAAGAAGAGGGTTTGGAGGACCTCGACTCTCACGCGCCTCCTCACCTACGCGCCATCGCAGACGCCACTCAGCTCGGCGACCTTGACGCCCTCCGACTCGCTCTCGATAACTTAGAAGGCAGTATTGATGAACCAGTGGAAGATGGGGACACAGCTCTGCATCTGGCTTGTCTCTATGGTAAACTTTCTTGTGCCCAGTTGCTTTTGGAGAGGGGGGCAAACTTGGAGGCTAAGGATGAAGATGGAGCTATTCCTCTGCATGATGCTTGTGCTGGGGGATACACTGAGATAGTTCAACTTCTAGTTAACAGTGCTAATGACACCGAGCGTGTAAAGAGAATGCTAGAAGCTGTTGACGCTGAAGGTGATACGCCTCTGCATCATGCTGCCAGAGGTGAGCATGCTGATATTATAAGATTGTTGCTGGCTTCTGGAGCTTCTCCTACAAGGGCAAACCTGTATGGAAAGATCCCAAGTGAGTTACCAGAACCGAACACAGAAGCTAAAAGGATTTTGGAGGCTGCTGCTGGTGCTATGGCATACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

21.59

Weight (kDa)

4.32

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 43 - 126 1.7e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 46 - 90 2.2e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 68 - 107 2.6e-07 Ankyrin repeats (many copies)
Ank PF00023 69 - 100 5.7e-09 Ankyrin repeat
Ank_4 PF13637 75 - 123 5.3e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 103 - 171 8.2e-12 Ankyrin repeats (3 copies)
Ank PF00023 143 - 173 1.5e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AccII CGCG 2 cut(s) 98, 114
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 126, 150
AfiI CCNNNNNNNGG 1 cut(s) 17
AgsI TTSAA 2 cut(s) 65, 359
AluBI AGCT 6 cut(s) 136, 218, 311, 413, 500, 566
AluI AGCT 6 cut(s) 136, 218, 311, 413, 500, 566
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 526
ApeKI GCWGC 3 cut(s) 449, 584, 587
AspLEI GCGC 2 cut(s) 100, 116
AspS9I GGNCC 1 cut(s) 82
AsuC2I CCSGG 1 cut(s) 12
AsuHPI GGTGA 3 cut(s) 98, 440, 470
AvaII GGWCC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 259
BbvI GCAGC 3 cut(s) 436, 571, 574
BccI CCATC 3 cut(s) 125, 200, 299
BciVI GTATCC 1 cut(s) 335
BcnI CCSGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 365, 407
BfuI GTATCC 1 cut(s) 335
BisI GCNGC 3 cut(s) 450, 585, 588
BlsI GCNGC 3 cut(s) 451, 586, 589
Bme1390I CCNGG 1 cut(s) 12
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 3 cut(s) 232, 316, 451
BmuI ACTGGG 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 126, 150
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 3 cut(s) 197, 259, 607
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 146, 339
BseNI ACTGG 3 cut(s) 197, 259, 607
BseRI GAGGAG 1 cut(s) 93
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 3 cut(s) 436, 571, 574
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 2 cut(s) 98, 114
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 1 cut(s) 531
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 145, 340
BspFNI CGCG 2 cut(s) 98, 114
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 1 cut(s) 526
BsrI ACTGG 3 cut(s) 197, 259, 607
BssMI GATC 1 cut(s) 531
BssNI GRCGYC 2 cut(s) 126, 150
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 2 cut(s) 126, 150
BstC8I GCNNGC 2 cut(s) 465, 489
BstDEI CTNAG 4 cut(s) 132, 175, 294, 348
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 2 cut(s) 98, 114
BstHHI GCGC 2 cut(s) 100, 116
BstKTI GATC 1 cut(s) 534
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 1 cut(s) 531
BstMWI GCNNNNNNNGC 3 cut(s) 419, 497, 593
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 259
BstUI CGCG 2 cut(s) 98, 114
BstV1I GCAGC 3 cut(s) 436, 571, 574
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BsuI GTATCC 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 204, 345, 379
Cac8I GCNNGC 2 cut(s) 465, 489
CfoI GCGC 2 cut(s) 100, 116
Cfr13I GGNCC 1 cut(s) 82
CseI GACGC 3 cut(s) 134, 158, 428
CviAII CATG 3 cut(s) 323, 446, 464
DdeI CTNAG 4 cut(s) 132, 175, 294, 348
DpnI GATC 1 cut(s) 533
DpnII GATC 1 cut(s) 531
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 82
FaeI CATG 3 cut(s) 326, 449, 467
FaiI YATR 8 cut(s) 240, 324, 447, 465, 476, 525, 599, 604
FaqI GGGAC 1 cut(s) 224
FatI CATG 3 cut(s) 322, 445, 463
Fnu4HI GCNGC 3 cut(s) 450, 585, 588
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 3 cut(s) 450, 585, 588
FspBI CTAG 2 cut(s) 365, 407
GlaI GCGC 2 cut(s) 99, 115
GluI GCNGC 3 cut(s) 450, 585, 588
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 516
HapII CCGG 1 cut(s) 11
HgaI GACGC 3 cut(s) 134, 158, 428
HhaI GCGC 2 cut(s) 100, 116
Hin1I GRCGYC 2 cut(s) 126, 150
Hin1II CATG 3 cut(s) 326, 449, 467
Hin6I GCGC 2 cut(s) 98, 114
HinP1I GCGC 2 cut(s) 98, 114
HincII GTYRAC 2 cut(s) 370, 418
HindII GTYRAC 2 cut(s) 370, 418
HinfI GANTC 2 cut(s) 89, 159
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 1 cut(s) 11
HphI GGTGA 3 cut(s) 98, 440, 470
Hpy166II GTNNAC 3 cut(s) 245, 370, 418
Hpy188I TCNGA 1 cut(s) 158
Hpy188III TCNNGA 2 cut(s) 167, 495
Hpy8I GTNNAC 3 cut(s) 245, 370, 418
HpyAV CCTTC 2 cut(s) 173, 419
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 223, 322, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 497, 593
HpyF3I CTNAG 4 cut(s) 132, 175, 294, 348
Hsp92I GRCGYC 2 cut(s) 126, 150
Hsp92II CATG 3 cut(s) 326, 449, 467
HspAI GCGC 2 cut(s) 98, 114
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 531
LmnI GCTCC 2 cut(s) 308, 497
Lsp1109I GCAGC 3 cut(s) 436, 571, 574
LweI GCATC 3 cut(s) 232, 316, 451
MaeI CTAG 2 cut(s) 365, 407
MaeIII GTNAC 1 cut(s) 543
MalI GATC 1 cut(s) 533
MboI GATC 1 cut(s) 531
MboII GAAGA 4 cut(s) 77, 80, 215, 314
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 1 cut(s) 259
MlyI GAGTC 2 cut(s) 83, 153
MmeI TCCRAC 1 cut(s) 181
MseI TTAA 1 cut(s) 369
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 408
MvnI CGCG 2 cut(s) 98, 114
MwoI GCNNNNNNNGC 3 cut(s) 419, 497, 593
NciI CCSGG 1 cut(s) 12
NdeII GATC 1 cut(s) 531
NlaIII CATG 3 cut(s) 326, 449, 467
NlaIV GGNNCC 1 cut(s) 9
NmeAIII GCCGAG 1 cut(s) 117
NspI RCATGY 1 cut(s) 467
PaeI GCATGC 1 cut(s) 467
PctI GAATGC 1 cut(s) 408
PkrI GCNGC 3 cut(s) 451, 586, 589
PleI GAGTC 2 cut(s) 83, 153
PpsI GAGTC 2 cut(s) 83, 153
PpuMI RGGWCCY 1 cut(s) 82
PsiI TTATAA 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 82
PspPPI RGGWCCY 1 cut(s) 82
PsuI RGATCY 1 cut(s) 531
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 450, 585, 588
Sau3AI GATC 1 cut(s) 531
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 83, 153
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 1 cut(s) 259
SfaNI GCATC 3 cut(s) 232, 316, 451
SinI GGWCC 1 cut(s) 82
SphI GCATGC 1 cut(s) 467
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 365, 407
StyD4I CCNGG 1 cut(s) 10
TaaI ACNGT 1 cut(s) 374
TaqI TCGA 2 cut(s) 87, 168
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 204, 352, 379
TseI GCWGC 3 cut(s) 449, 584, 587
TspDTI ATGAA 2 cut(s) 207, 315
TspRI CASTG 3 cut(s) 204, 352, 379
VpaK11BI GGWCC 1 cut(s) 82
XceI RCATGY 1 cut(s) 467
XspI CTAG 2 cut(s) 365, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.