Rorug03G0173100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007223
Physical Location & Seq
Reverse (-)
14625782 .. 14641998
16217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0173100.1

Sequence Viewer

Length: 837 bp
ATGCTATATCTATTATTCATTGCAGACGTAGTGATGGTTAAATCAAAGAAATTCCATCTTCAGTTCTTTGCTTTCACTCTTTTTCTTCTGTCCTGGCACCCCATCAGACCCGTCAGAGGCCAAGGAGTGGAAGTAATGGAGTATGATTATAGGCAAGGGAGCCCCAAGGGACCAGAGCACTGGGGAGAGCTCAACGAAGAATTGGAGCAATGCAAAGATGGAAAATTACAATCGCCAATAGATTTGTTCTATTCGAATGCTAGAAAAGCAATCTCAAGAGTAGATGATCTTAACATGAGCTATAAGCCTTCAAAAGCAACTATGAAGCATGAACACCATGCTATTTCGATTCAATGGGAGGGTGATGCTGGATCAATTCAAATAAATGGCACTGATTACTTGCTCAAAAGATGTCATTGGCACAGATCCTCTGAACATTTCATTAATGGCAGAAGTTATGATTTGGAATTGCACATGGTTCACCAAAGCCAGAACAACGATGTGGCTGTAGTCGCATTCCTATACCAAAGTGGCAATCCCGATCCTTTCCTGTCAAAGATAAGCAACGATGTATCCTCCCTAAGTGGCACAAAGGAGGCACGCTTGGGAGTGATTGATCCTAGTTTAATAAAATGGCCAAGCTCACGATTTTACAGATATATGGGCTCACTGACGACCCCTCCTTGCACTGAAGGAGTTATTTGGAGCGTATACGAAGAGGTACATTCTGTTTCAATAGAACAGCTGGGGCTAATTGAACAAGTCGATCATGGGATGAATGCAAGACCATTACAGCCTCTTAAGAATCGAGATATCACGCTCTTTGGTCCGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

31.84

Weight (kDa)

6.35

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 56 - 273 5.8e-35 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 127
AccI GTMKAC 1 cut(s) 711
AciI CCGC 1 cut(s) 830
AclWI GGATC 4 cut(s) 379, 420, 536, 611
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 2 cut(s) 44, 711
AfaI GTAC 1 cut(s) 723
AfiI CCNNNNNNNGG 2 cut(s) 116, 127
AflII CTTAAG 1 cut(s) 800
AgsI TTSAA 5 cut(s) 312, 353, 380, 735, 758
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 4 cut(s) 190, 300, 642, 745
AluI AGCT 4 cut(s) 190, 300, 642, 745
Alw21I GWGCWC 2 cut(s) 180, 192
AlwI GGATC 4 cut(s) 379, 420, 536, 611
AoxI GGCC 2 cut(s) 118, 635
ApoI RAATTY 1 cut(s) 50
AseI ATTAAT 1 cut(s) 444
AspS9I GGNCC 2 cut(s) 170, 827
AsuHPI GGTGA 2 cut(s) 374, 473
AsuII TTCGAA 1 cut(s) 254
AvaII GGWCC 2 cut(s) 170, 827
BalI TGGCCA 1 cut(s) 637
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 3 cut(s) 164, 192, 668
Bbv12I GWGCWC 2 cut(s) 180, 192
BccI CCATC 4 cut(s) 28, 63, 110, 212
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 583
BfaI CTAG 2 cut(s) 261, 621
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 800
BfuI GTATCC 1 cut(s) 583
Bme1390I CCNGG 1 cut(s) 94
Bme18I GGWCC 2 cut(s) 170, 827
BmgT120I GGNCC 2 cut(s) 170, 827
BmiI GGNNCC 3 cut(s) 98, 161, 171
BmrFI CCNGG 1 cut(s) 94
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 190
Bpu14I TTCGAA 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 259
BsaJI CCNNGG 2 cut(s) 121, 165
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 127
Bse1I ACTGG 1 cut(s) 185
Bse3DI GCAATG 2 cut(s) 18, 215
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 2 cut(s) 121, 165
BseGI GGATG 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 116, 127
BseMI GCAATG 2 cut(s) 18, 215
BseNI ACTGG 1 cut(s) 185
BseYI CCCAGC 1 cut(s) 745
BshFI GGCC 2 cut(s) 120, 637
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 2 cut(s) 180, 192
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 2 cut(s) 116, 127
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 3 cut(s) 262, 515, 784
BsnI GGCC 2 cut(s) 120, 637
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 4 cut(s) 164, 180, 192, 668
Bsp143I GATC 6 cut(s) 286, 371, 425, 541, 616, 766
BspACI CCGC 1 cut(s) 830
BspANI GGCC 2 cut(s) 120, 637
BspLI GGNNCC 3 cut(s) 98, 161, 171
BspPI GGATC 4 cut(s) 379, 420, 536, 611
BspT104I TTCGAA 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 1 cut(s) 800
BsrDI GCAATG 2 cut(s) 18, 215
BsrI ACTGG 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 121, 165
BssMI GATC 6 cut(s) 286, 371, 425, 541, 616, 766
BssNAI GTATAC 1 cut(s) 712
BssT1I CCWWGG 2 cut(s) 121, 165
Bst1107I GTATAC 1 cut(s) 712
Bst2UI CCWGG 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 711
BstAFI CTTAAG 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 581
BstF5I GGATG 1 cut(s) 780
BstKTI GATC 6 cut(s) 289, 374, 428, 544, 619, 769
BstMBI GATC 6 cut(s) 286, 371, 425, 541, 616, 766
BstMWI GCNNNNNNNGC 2 cut(s) 266, 512
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 425
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 425
BstZ17I GTATAC 1 cut(s) 712
BsuI GTATCC 1 cut(s) 583
BsuRI GGCC 2 cut(s) 120, 637
BtsCI GGATG 1 cut(s) 780
BtsIMutI CAGTG 4 cut(s) 178, 390, 668, 687
Cac8I GCNNGC 1 cut(s) 601
Cfr13I GGNCC 2 cut(s) 170, 827
Csp6I GTAC 1 cut(s) 722
CviAII CATG 5 cut(s) 295, 329, 338, 475, 770
CviQI GTAC 1 cut(s) 722
DdeI CTNAG 1 cut(s) 581
DpnI GATC 6 cut(s) 288, 373, 427, 543, 618, 768
DpnII GATC 6 cut(s) 286, 371, 425, 541, 616, 766
EaeI YGGCCR 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 121, 165
Eco24I GRGCYC 3 cut(s) 164, 192, 668
Eco32I GATATC 1 cut(s) 814
Eco47I GGWCC 2 cut(s) 170, 827
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 44, 711
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 92
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 121, 165
EcoT38I GRGCYC 3 cut(s) 164, 192, 668
ErhI CCWWGG 2 cut(s) 121, 165
FaeI CATG 5 cut(s) 298, 332, 341, 478, 773
FaqI GGGAC 1 cut(s) 183
FatI CATG 5 cut(s) 294, 328, 337, 474, 769
FblI GTMKAC 1 cut(s) 711
FokI GGATG 1 cut(s) 787
FriOI GRGCYC 3 cut(s) 164, 192, 668
FspBI CTAG 2 cut(s) 261, 621
GsaI CCCAGC 1 cut(s) 749
HaeIII GGCC 2 cut(s) 120, 637
Hin1II CATG 5 cut(s) 298, 332, 341, 478, 773
HinfI GANTC 2 cut(s) 349, 805
HphI GGTGA 2 cut(s) 374, 473
Hpy166II GTNNAC 2 cut(s) 481, 712
Hpy188I TCNGA 3 cut(s) 107, 116, 433
Hpy188III TCNNGA 4 cut(s) 276, 539, 645, 809
Hpy8I GTNNAC 2 cut(s) 481, 712
HpyAV CCTTC 2 cut(s) 318, 686
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 5 cut(s) 23, 213, 472, 687, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 512
HpyF3I CTNAG 1 cut(s) 581
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 5 cut(s) 298, 332, 341, 478, 773
Kzo9I GATC 6 cut(s) 286, 371, 425, 541, 616, 766
LmnI GCTCC 3 cut(s) 159, 205, 705
LpnPI CCDG 8 cut(s) 79, 106, 166, 186, 354, 503, 563, 731
LweI GCATC 1 cut(s) 355
MaeI CTAG 2 cut(s) 261, 621
MaeII ACGT 1 cut(s) 27
MalI GATC 6 cut(s) 288, 373, 427, 543, 618, 768
MboI GATC 6 cut(s) 286, 371, 425, 541, 616, 766
MboII GAAGA 4 cut(s) 50, 77, 209, 728
MflI RGATCY 1 cut(s) 425
MhlI GDGCHC 4 cut(s) 164, 180, 192, 668
MlsI TGGCCA 1 cut(s) 637
MluCI AATT 6 cut(s) 50, 200, 224, 375, 467, 753
MluNI TGGCCA 1 cut(s) 637
MnlI CCTC 8 cut(s) 110, 352, 439, 586, 589, 690, 712, 807
Mox20I TGGCCA 1 cut(s) 637
MscI TGGCCA 1 cut(s) 637
MseI TTAA 5 cut(s) 39, 291, 444, 626, 801
Msp20I TGGCCA 1 cut(s) 637
MspA1I CMGCKG 1 cut(s) 745
MspCI CTTAAG 1 cut(s) 800
MspR9I CCNGG 1 cut(s) 94
Mva1269I GAATGC 3 cut(s) 262, 515, 784
MvaI CCWGG 1 cut(s) 94
MwoI GCNNNNNNNGC 2 cut(s) 266, 512
NdeII GATC 6 cut(s) 286, 371, 425, 541, 616, 766
NlaIII CATG 5 cut(s) 298, 332, 341, 478, 773
NlaIV GGNNCC 3 cut(s) 98, 161, 171
NspV TTCGAA 1 cut(s) 254
PctI GAATGC 3 cut(s) 262, 515, 784
PfeI GAWTC 2 cut(s) 349, 805
PflMI CCANNNNNTGG 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 745
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 3 cut(s) 98, 161, 171
PspPI GGNCC 2 cut(s) 170, 827
PsuI RGATCY 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 745
RsaI GTAC 1 cut(s) 723
RsaNI GTAC 1 cut(s) 722
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 5 cut(s) 39, 291, 444, 626, 801
Sau3AI GATC 6 cut(s) 286, 371, 425, 541, 616, 766
Sau96I GGNCC 2 cut(s) 170, 827
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 4 cut(s) 164, 180, 192, 668
SetI ASST 6 cut(s) 30, 192, 302, 644, 723, 747
SfaNI GCATC 1 cut(s) 355
SfcI CTRYAG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 2 cut(s) 170, 827
SmlI CTYRAG 2 cut(s) 274, 800
SmoI CTYRAG 2 cut(s) 274, 800
Sse9I AATT 6 cut(s) 50, 200, 224, 375, 467, 753
SsiI CCGC 1 cut(s) 830
SspMI CTAG 2 cut(s) 261, 621
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 2 cut(s) 121, 165
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 254, 347, 765, 808
TasI AATT 6 cut(s) 50, 200, 224, 375, 467, 753
TfiI GAWTC 2 cut(s) 349, 805
Tru1I TTAA 5 cut(s) 39, 291, 444, 626, 801
Tru9I TTAA 5 cut(s) 39, 291, 444, 626, 801
TscAI CASTG 4 cut(s) 185, 397, 675, 694
TspDTI ATGAA 5 cut(s) 7, 338, 345, 430, 791
TspRI CASTG 4 cut(s) 185, 397, 675, 694
Van91I CCANNNNNTGG 1 cut(s) 127
Vha464I CTTAAG 1 cut(s) 800
VpaK11BI GGWCC 2 cut(s) 170, 827
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 2 cut(s) 261, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.