MD12G1062300.v1.1

protein C4orf29 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013448
Physical Location & Seq
Reverse (-)
7301210 .. 7303778
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1062300.v1.1.491

Sequence Viewer

Length: 942 bp
ATGGTGACTGTGAATCTGGGAATGCTCCATTATGTTCTGGACCATGTGTATGGTGCATTCATGCACAGAACGAAGATAAGCCCTCAATTTTTTTCGAGAGGGTGGGGCGGCTCAAAGCTTGAGTTGCTTGAGAGGATGATAAAGCAGCTTTTCCCAGAAGCAGCTGGCCAGAATTGGCCTCCCGCTGTGATCCAACCCACTTGGAAAACTGTTTGGGAGAGCAAGACGGCTTCTCTCAGAGAAGGGATCTTCAGGACGCCTTGTGATGAGCAGCTTCTCGGTGCGTTGCCTCCTGAGAGTCACCGTGCAAGAGTTGCATTTCTTGTTCCGAAGTCGGTTCCTCCTCAGAGAATGGCCTGTGTTGTTCATCTTGCAGGAACTGGGGACCATACATTTGAACGAAGGTTACGTCTTGGTGGACCGTTGCTGAAGGAAAACATTGCTACTATGGTGCTTGAGAGCCCTTTCTATGGGCAAAGACGTCCTGTAATGCAGCGTGGGGCAAGGCTCTTGTGTGTTAGTGACTTGCTTTTATTAGGAAAGGCAACCATTGAAGAAGCTCGCAGTCTCTTGCATTGGTTGGACTGTGAGGCGGGATTTGGCAAGATGGGTGTTTGTGGACTTAGTATGGTACATGCTGCGATGGTTGGATCACTGCACCCTACACCTGTTGCAACCCTTCCTTTTCTTTCTCCACACTCAGCTGTTGTGGCATTCTGTGAGGGAATATTACAGCATGCAACTGCATGGGAGGCACTGAGAGATGATCTTGCAGTGCAGAAGGCTGCAATGACTCTCGAGGAGGTGAGAGAACGGATGCGTAATGTCCTGTCTCTCACAGATGTCACACGCTTTCCGATTCCAAAAAATCCTAATGCTGTGATATTTGTTTCTGCCACTGTAAGTACTTATGAGGTTACCTTTCTACCCATTTGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2048

Protein Analysis

314

Amino Acids

34.78

Weight (kDa)

8.86

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABHD18 PF09752 29 - 304 5.6e-94 Alpha/beta hydrolase domain containing 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013448)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12150
fragaria_vesca FvH4_6g22800
malus_domestica MD12G1062300.v1.1
prunus_persica Prupe.7G072500_v2.0.a1 Prupe.7G072500_v2.0.a1
pyrus_communis pycom12g05500 pycom14g05050
rosa_chinensis RchiOBHm_Chr3g0480421
rosa_laevigata RLG00000023483
rosa_multiflora Rmu_sc0002205.1_g000024
rosa_roxburghii Rroxscaffold_6G00401370
rosa_rugosa Rorug03G0183800 Rorug03G0183900
rosa_samantha Rh3AG235300 Rh3BG268200 Rh3CG265300 Rh3DG261200
rosa_wichuraiana Rw3G021120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 484
AciI CCGC 3 cut(s) 108, 183, 593
AclWI GGATC 3 cut(s) 184, 254, 658
AcoI YGGCCR 1 cut(s) 166
AcuI CTGAAG 2 cut(s) 235, 449
AcyI GRCGYC 2 cut(s) 257, 481
AfaI GTAC 2 cut(s) 633, 907
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 2 cut(s) 398, 554
AluBI AGCT 6 cut(s) 118, 148, 164, 274, 560, 704
AluI AGCT 6 cut(s) 118, 148, 164, 274, 560, 704
Alw26I GTCTC 2 cut(s) 572, 837
AlwI GGATC 3 cut(s) 184, 254, 658
AlwNI CAGNNNCTG 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 3 cut(s) 166, 176, 354
ApeKI GCWGC 6 cut(s) 145, 161, 271, 493, 638, 785
ArsI GACNNNNNNTTYG 4 cut(s) 377, 394, 409, 426
AspS9I GGNCC 3 cut(s) 40, 385, 419
AsuHPI GGTGA 3 cut(s) 16, 293, 817
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 3 cut(s) 40, 385, 419
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 464
BbvI GCAGC 6 cut(s) 157, 173, 283, 505, 625, 772
BccI CCATC 2 cut(s) 601, 637
BceAI ACGGC 1 cut(s) 243
BcoDI GTCTC 2 cut(s) 572, 837
BisI GCNGC 7 cut(s) 109, 146, 162, 272, 494, 639, 786
BlsI GCNGC 7 cut(s) 110, 147, 163, 273, 495, 640, 787
BmcAI AGTACT 1 cut(s) 907
Bme18I GGWCC 3 cut(s) 40, 385, 419
BmeT110I CYCGRG 1 cut(s) 797
BmgT120I GGNCC 3 cut(s) 40, 385, 419
BmiI GGNNCC 2 cut(s) 339, 386
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 807
BmuI ACTGGG 1 cut(s) 390
BpuEI CTTGAG 3 cut(s) 140, 149, 476
BsaHI GRCGYC 2 cut(s) 257, 481
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 385
Bse3DI GCAATG 2 cut(s) 438, 795
BseGI GGATG 2 cut(s) 141, 822
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 2 cut(s) 438, 795
BseMII CTCAG 5 cut(s) 250, 285, 359, 714, 749
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 2 cut(s) 333, 815
BseXI GCAGC 6 cut(s) 157, 173, 283, 505, 625, 772
BsgI GTGCAG 2 cut(s) 641, 797
BshFI GGCC 3 cut(s) 168, 178, 356
BsiHKCI CYCGRG 1 cut(s) 797
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 572, 837
BsmFI GGGAC 1 cut(s) 398
BsmI GAATGC 3 cut(s) 27, 56, 713
BsnI GGCC 3 cut(s) 168, 178, 356
BsoBI CYCGRG 1 cut(s) 797
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 4 cut(s) 189, 246, 650, 766
BspACI CCGC 3 cut(s) 108, 183, 593
BspANI GGCC 3 cut(s) 168, 178, 356
BspCNI CTCAG 5 cut(s) 249, 286, 358, 713, 750
BspLI GGNNCC 2 cut(s) 339, 386
BspPI GGATC 3 cut(s) 184, 254, 658
BsrDI GCAATG 2 cut(s) 438, 795
BsrI ACTGG 1 cut(s) 385
BssMI GATC 4 cut(s) 189, 246, 650, 766
BssNI GRCGYC 2 cut(s) 257, 481
Bst4CI ACNGT 6 cut(s) 10, 211, 305, 423, 587, 901
BstACI GRCGYC 2 cut(s) 257, 481
BstAPI GCANNNNNTGC 1 cut(s) 314
BstC8I GCNNGC 3 cut(s) 166, 562, 738
BstDEI CTNAG 6 cut(s) 236, 294, 345, 623, 700, 758
BstEII GGTNACC 1 cut(s) 916
BstF5I GGATG 2 cut(s) 141, 822
BstKTI GATC 4 cut(s) 192, 249, 653, 769
BstMAI GTCTC 2 cut(s) 572, 837
BstMBI GATC 4 cut(s) 189, 246, 650, 766
BstMWI GCNNNNNNNGC 4 cut(s) 124, 314, 710, 752
BstNSI RCATGY 2 cut(s) 638, 740
BstPI GGTNACC 1 cut(s) 916
BstV1I GCAGC 6 cut(s) 157, 173, 283, 505, 625, 772
BstX2I RGATCY 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 50
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 3 cut(s) 168, 178, 356
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 2 cut(s) 141, 822
BtsI GCAGTG 2 cut(s) 653, 780
BtsIMutI CAGTG 4 cut(s) 653, 755, 780, 897
Cac8I GCNNGC 3 cut(s) 166, 562, 738
CaiI CAGNNNCTG 1 cut(s) 380
Cfr13I GGNCC 3 cut(s) 40, 385, 419
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 632, 906
CviAII CATG 5 cut(s) 44, 61, 635, 737, 747
CviQI GTAC 2 cut(s) 632, 906
DdeI CTNAG 6 cut(s) 236, 294, 345, 623, 700, 758
DpnI GATC 4 cut(s) 191, 248, 652, 768
DpnII GATC 4 cut(s) 189, 246, 650, 766
EaeI YGGCCR 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 464
Eco47I GGWCC 3 cut(s) 40, 385, 419
Eco57I CTGAAG 2 cut(s) 235, 449
Eco88I CYCGRG 1 cut(s) 797
Eco91I GGTNACC 1 cut(s) 916
EcoO65I GGTNACC 1 cut(s) 916
EcoT38I GRGCYC 1 cut(s) 464
FaeI CATG 5 cut(s) 47, 64, 638, 740, 750
FaqI GGGAC 1 cut(s) 398
FatI CATG 5 cut(s) 43, 60, 634, 736, 746
FauI CCCGC 2 cut(s) 190, 586
Fnu4HI GCNGC 7 cut(s) 109, 146, 162, 272, 494, 639, 786
FokI GGATG 2 cut(s) 148, 829
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 7 cut(s) 109, 146, 162, 272, 494, 639, 786
GluI GCNGC 7 cut(s) 109, 146, 162, 272, 494, 639, 786
HaeIII GGCC 3 cut(s) 168, 178, 356
HgaI GACGC 1 cut(s) 265
Hin1I GRCGYC 2 cut(s) 257, 481
Hin1II CATG 5 cut(s) 47, 64, 638, 740, 750
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 4 cut(s) 13, 298, 793, 859
HphI GGTGA 3 cut(s) 16, 293, 817
Hpy166II GTNNAC 2 cut(s) 419, 620
Hpy188I TCNGA 4 cut(s) 239, 330, 348, 858
Hpy188III TCNNGA 5 cut(s) 38, 96, 253, 293, 797
Hpy8I GTNNAC 2 cut(s) 419, 620
HpyAV CCTTC 5 cut(s) 236, 396, 424, 689, 775
HpyCH4III ACNGT 6 cut(s) 10, 211, 305, 423, 587, 901
HpyCH4IV ACGT 2 cut(s) 409, 481
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 314, 710, 752
HpyF3I CTNAG 6 cut(s) 236, 294, 345, 623, 700, 758
HpySE526I ACGT 2 cut(s) 409, 481
Hsp92I GRCGYC 2 cut(s) 257, 481
Hsp92II CATG 5 cut(s) 47, 64, 638, 740, 750
Kzo9I GATC 4 cut(s) 189, 246, 650, 766
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 6 cut(s) 157, 173, 283, 505, 625, 772
LweI GCATC 1 cut(s) 807
MaeII ACGT 2 cut(s) 409, 481
MaeIII GTNAC 6 cut(s) 4, 299, 405, 521, 844, 916
MalI GATC 4 cut(s) 191, 248, 652, 768
MboI GATC 4 cut(s) 189, 246, 650, 766
MboII GAAGA 3 cut(s) 85, 241, 566
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 464
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 2 cut(s) 86, 172
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 2 cut(s) 307, 787
MmeI TCCRAC 3 cut(s) 217, 561, 628
Mox20I TGGCCA 1 cut(s) 168
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 940
MslI CAYNNNNRTG 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 168
MspA1I CMGCKG 3 cut(s) 164, 185, 704
Mva1269I GAATGC 3 cut(s) 27, 56, 713
MwoI GCNNNNNNNGC 4 cut(s) 124, 314, 710, 752
NdeII GATC 4 cut(s) 189, 246, 650, 766
NlaIII CATG 5 cut(s) 47, 64, 638, 740, 750
NlaIV GGNNCC 2 cut(s) 339, 386
NmuCI GTSAC 4 cut(s) 4, 299, 521, 844
NspI RCATGY 2 cut(s) 638, 740
PaeI GCATGC 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 797
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PctI GAATGC 3 cut(s) 27, 56, 713
PfeI GAWTC 2 cut(s) 13, 859
PkrI GCNGC 7 cut(s) 110, 147, 163, 273, 495, 640, 787
PleI GAGTC 2 cut(s) 306, 787
PpsI GAGTC 2 cut(s) 306, 787
PspEI GGTNACC 1 cut(s) 916
PspN4I GGNNCC 2 cut(s) 339, 386
PspPI GGNCC 3 cut(s) 40, 385, 419
PsrI GAACNNNNNNTAC 2 cut(s) 390, 422
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 1 cut(s) 246
PvuII CAGCTG 2 cut(s) 164, 704
RsaI GTAC 2 cut(s) 633, 907
RsaNI GTAC 2 cut(s) 632, 906
RseI CAYNNNNRTG 1 cut(s) 48
SaqAI TTAA 1 cut(s) 940
SatI GCNGC 7 cut(s) 109, 146, 162, 272, 494, 639, 786
Sau3AI GATC 4 cut(s) 189, 246, 650, 766
Sau96I GGNCC 3 cut(s) 40, 385, 419
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 2 cut(s) 307, 787
SduI GDGCHC 1 cut(s) 464
SfaNI GCATC 1 cut(s) 807
Sfr274I CTCGAG 1 cut(s) 797
SinI GGWCC 3 cut(s) 40, 385, 419
SlaI CTCGAG 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 4 cut(s) 119, 128, 455, 797
SmoI CTYRAG 4 cut(s) 119, 128, 455, 797
SphI GCATGC 1 cut(s) 740
Sse9I AATT 2 cut(s) 86, 172
SsiI CCGC 3 cut(s) 108, 183, 593
SspI AATATT 1 cut(s) 729
TaaI ACNGT 6 cut(s) 10, 211, 305, 423, 587, 901
TaiI ACGT 2 cut(s) 412, 484
TaqI TCGA 2 cut(s) 95, 798
TasI AATT 2 cut(s) 86, 172
TatI WGTACW 1 cut(s) 905
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 2 cut(s) 13, 859
Tru1I TTAA 1 cut(s) 940
Tru9I TTAA 1 cut(s) 940
TscAI CASTG 4 cut(s) 660, 762, 780, 904
TseFI GTSAC 4 cut(s) 4, 299, 521, 844
TseI GCWGC 6 cut(s) 145, 161, 271, 493, 638, 785
Tsp45I GTSAC 4 cut(s) 4, 299, 521, 844
TspDTI ATGAA 2 cut(s) 49, 356
TspGWI ACGGA 1 cut(s) 829
TspRI CASTG 4 cut(s) 660, 762, 780, 904
VpaK11BI GGWCC 3 cut(s) 40, 385, 419
XceI RCATGY 2 cut(s) 638, 740
XhoI CTCGAG 1 cut(s) 797
ZraI GACGTC 1 cut(s) 482
ZrmI AGTACT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.